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Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA1

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')-I
Short name:
APH(3')I
Kanamycin kinase, type I
Neomycin-kanamycin phosphotransferase type I
Gene namesi
Name:aphA1
Synonyms:nptI
Encoded oniPlasmid Rts11 Publication
Plasmid pIP15181 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Chemistry

DrugBankiDB01172. Kanamycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Aminoglycoside 3'-phosphotransferasePRO_0000204802Add
BLAST

Proteomic databases

PRIDEiP00551.

Interactioni

Protein-protein interaction databases

IntActiP00551. 2 interactions.
MINTiMINT-6478052.

Structurei

3D structure databases

ProteinModelPortaliP00551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00897.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21310. PTHR21310. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P00551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP
60 70 80 90 100
ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
110 120 130 140 150
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA
160 170 180 190 200
QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
210 220 230 240 250
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK
260 270
YGIDNPDMNK LQFHLMLDEF F
Length:271
Mass (Da):30,961
Last modified:June 7, 2005 - v2
Checksum:i3B715D9BBE8031B8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191L → M (PubMed:6270337).Curated
Sequence conflicti19 – 191L → M (Ref. 4) Curated
Sequence conflicti48 – 481D → N in CAA68507 (PubMed:2823223).Curated
Sequence conflicti120 – 1201I → N in CAA68507 (PubMed:2823223).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti27 – 271K → R in plasmid Rts1 and strain X.
Natural varianti77 – 771E → A in plasmid Rts1 and strain X.
Natural varianti126 – 1261V → A in plasmid Rts1.
Natural varianti150 – 1501A → T in plasmid Rts1.
Natural varianti247 – 2471L → F in plasmid Rts1.
Natural varianti249 – 2491Q → Y in plasmid Rts1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00359 Genomic DNA. Translation: CAA23656.1.
Y00452 Genomic DNA. Translation: CAA68507.1.
M65202 Unassigned DNA. Translation: AAA72095.1.
AF498082 Genomic DNA. Translation: AAN59785.1.
S54065 Genomic DNA. Translation: AAD13871.1.
V00621 Genomic DNA. Translation: CAA23898.1.
M10596 Genomic DNA. Translation: AAA27445.1.
M10597 Genomic DNA. Translation: AAA27446.1.
V00293 Genomic DNA. Translation: CAA23568.1.
PIRiA00662. PKECT9.
RefSeqiWP_000018322.1. NZ_JMIZ01000008.1.
YP_001687822.1. NC_004429.1.
YP_002891173.1. NC_012690.1.
YP_002995708.1. NC_012886.1.
YP_008864028.1. NC_022992.1.
YP_008864695.1. NC_022996.1.
YP_008995202.1. NC_023277.2.
YP_008997405.1. NC_023289.2.
YP_009074178.1. NG_034499.1.
YP_009084339.1. NG_037755.1.
YP_009088384.1. NG_041041.1.

Genome annotation databases

GeneIDi17824309.
17824444.
18157677.
18157927.
5951155.
7872406.
8094921.
KEGGipg:17824309.
pg:17824444.
pg:18157677.
pg:18157927.
pg:5951155.
pg:7872406.
pg:8094921.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00359 Genomic DNA. Translation: CAA23656.1.
Y00452 Genomic DNA. Translation: CAA68507.1.
M65202 Unassigned DNA. Translation: AAA72095.1.
AF498082 Genomic DNA. Translation: AAN59785.1.
S54065 Genomic DNA. Translation: AAD13871.1.
V00621 Genomic DNA. Translation: CAA23898.1.
M10596 Genomic DNA. Translation: AAA27445.1.
M10597 Genomic DNA. Translation: AAA27446.1.
V00293 Genomic DNA. Translation: CAA23568.1.
PIRiA00662. PKECT9.
RefSeqiWP_000018322.1. NZ_JMIZ01000008.1.
YP_001687822.1. NC_004429.1.
YP_002891173.1. NC_012690.1.
YP_002995708.1. NC_012886.1.
YP_008864028.1. NC_022992.1.
YP_008864695.1. NC_022996.1.
YP_008995202.1. NC_023277.2.
YP_008997405.1. NC_023289.2.
YP_009074178.1. NG_034499.1.
YP_009084339.1. NG_037755.1.
YP_009088384.1. NG_041041.1.

3D structure databases

ProteinModelPortaliP00551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00551. 2 interactions.
MINTiMINT-6478052.

Chemistry

DrugBankiDB01172. Kanamycin.

Proteomic databases

PRIDEiP00551.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17824309.
17824444.
18157677.
18157927.
5951155.
7872406.
8094921.
KEGGipg:17824309.
pg:17824444.
pg:18157677.
pg:18157927.
pg:5951155.
pg:7872406.
pg:8094921.

Phylogenomic databases

KOiK00897.

Family and domain databases

InterProiIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21310. PTHR21310. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the kanamycin resistance transposon Tn903."
    Oka A., Sugisaki H., Takanami M.
    J. Mol. Biol. 147:217-226(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn903.
  2. "The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene from E. coli."
    Vakulenko S., Kalman M., Horvath B., Simoncsits A.
    Nucleic Acids Res. 15:8111-8111(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: X.
  3. "Three short fragments of Rts1 DNA are responsible for the temperature-sensitive growth phenotype (Tsg) of host bacteria."
    Mochida S., Tsuchiya H., Mori K., Kaji A.
    J. Bacteriol. 173:2600-2607(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: Rts1
  4. Press C.M., Mahaffee W.F., Roche M.M.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Overproduction of 3'-aminoglycoside phosphotransferase type I confers resistance to tobramycin in Escherichia coli."
    Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R.
    Antimicrob. Agents Chemother. 37:78-83(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
    Strain: HM69.
    Plasmid: pIP1518
  6. "Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903."
    Grindley N.D.F., Joyce C.M.
    Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Transposon: Tn903.
  7. "Analysis of the structure and function of the kanamycin-resistance transposon Tn903."
    Grindley N.D.F., Joyce C.M.
    Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271.
    Transposon: Tn903.
  8. "Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication."
    Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L., Jansz H.S.
    Nucleic Acids Res. 9:3335-3354(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.

Entry informationi

Entry nameiKKA1_ECOLX
AccessioniPrimary (citable) accession number: P00551
Secondary accession number(s): Q53299, Q8GNQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: May 27, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is encoded by the kanamycin resistance transposon Tn903.

Caution

PubMed:2823223 strain is not known and has been termed 'X' in this entry.Curated
Was originally (Ref. 4) termed APH(3')IIa whereas it is APH(3')Ia.Curated

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.