P00551 (KKA1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminoglycoside 3'-phosphotransferase
EC=2.7.1.95

Alternative name(s):
APH(3')-I
Short name=APH(3')I
Kanamycin kinase, type I
Neomycin-kanamycin phosphotransferase type I

Gene names

Name:	aphA1
Synonyms:	nptI

Encoded on

Plasmid Rts1 Ref.3
Plasmid pIP1518 Ref.5

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.
Catalytic activity	ATP + kanamycin = ADP + kanamycin 3'-phosphate.
Miscellaneous	This enzyme is encoded by the kanamycin resistance transposon Tn903.
Sequence similarities	Belongs to the aminoglycoside phosphotransferase family.
Caution	Ref.2 strain is not known and has been termed 'X' in this entry. Was originally (Ref.4) termed APH(3')IIa whereas it is APH(3')Ia.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Plasmid Transposable element
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kanamycin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Aminoglycoside 3'-phosphotransferase		PRO_0000204802
Sites
	Active site	198	1	Proton acceptor By similarity
Natural variations
	Natural variant	27	1	K → R in plasmid Rts1 and strain X.
	Natural variant	77	1	E → A in plasmid Rts1 and strain X.
	Natural variant	126	1	V → A in plasmid Rts1.
	Natural variant	150	1	A → T in plasmid Rts1.
	Natural variant	247	1	L → F in plasmid Rts1.
	Natural variant	249	1	Q → Y in plasmid Rts1.
Experimental info
	Sequence conflict	19	1	L → M Ref.1
	Sequence conflict	19	1	L → M Ref.4
	Sequence conflict	48	1	D → N in CAA68507. Ref.2
	Sequence conflict	120	1	I → N in CAA68507. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00551 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 3B715D9BBE8031B8	FASTA	271	30,961
	10 20 30 40 50 60 MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS 70 80 90 100 110 120 VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI 130 140 150 160 170 180 VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK 190 200 210 220 230 240 EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS 250 260 270 PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F

References

[1]	"Nucleotide sequence of the kanamycin resistance transposon Tn903." Oka A., Sugisaki H., Takanami M. J. Mol. Biol. 147:217-226(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Transposon: Tn903.
[2]	"The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene from E. coli." Vakulenko S., Kalman M., Horvath B., Simoncsits A. Nucleic Acids Res. 15:8111-8111(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: X.
[3]	"Three short fragments of Rts1 DNA are responsible for the temperature-sensitive growth phenotype (Tsg) of host bacteria." Mochida S., Tsuchiya H., Mori K., Kaji A. J. Bacteriol. 173:2600-2607(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Press C.M., Mahaffee W.F., Roche M.M. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Overproduction of 3'-aminoglycoside phosphotransferase type I confers resistance to tobramycin in Escherichia coli." Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R. Antimicrob. Agents Chemother. 37:78-83(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42. Strain: HM69.
[6]	"Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903." Grindley N.D.F., Joyce C.M. Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Transposon: Tn903.
[7]	"Analysis of the structure and function of the kanamycin-resistance transposon Tn903." Grindley N.D.F., Joyce C.M. Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271. Transposon: Tn903.
[8]	"Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication." Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L., Jansz H.S. Nucleic Acids Res. 9:3335-3354(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00359 Genomic DNA. Translation: CAA23656.1. Y00452 Genomic DNA. Translation: CAA68507.1. M65202 Unassigned DNA. Translation: AAA72095.1. AF498082 Genomic DNA. Translation: AAN59785.1. S54065 Genomic DNA. Translation: AAD13871.1. V00621 Genomic DNA. Translation: CAA23898.1. M10596 Genomic DNA. Translation: AAA27445.1. M10597 Genomic DNA. Translation: AAA27446.1. V00293 Genomic DNA. Translation: CAA23568.1.
PIR	PKECT9. A00662.
RefSeq	YP_001687822.1. NC_004429.1. YP_002891173.1. NC_012690.1. YP_002995708.1. NC_012886.1.
3D structure databases
ProteinModelPortal	P00551.
ModBase	Search...
Protein-protein interaction databases
IntAct	P00551. 1 interaction.
Proteomic databases
PRIDE	P00551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5951155. 7872406. 8094921.
Phylogenomic databases
ProtClustDB	CLSK2517079.
Enzyme and pathway databases
SABIO-RK	P00551.
Family and domain databases
InterPro	IPR002575. Aminoglycoside_PTrfase. IPR024165. Kan/Strep_kinase. IPR011009. Kinase-like_dom. [Graphical view]
PANTHER	PTHR21310. PTHR21310. 1 hit.
Pfam	PF01636. APH. 1 hit. [Graphical view]
PIRSF	PIRSF000706. Kanamycin_kin. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB01172. Kanamycin.

Entry information

Entry name

KKA1_ECOLX

Accession

Primary (citable) accession number: P00551
Secondary accession number(s): Q53299, Q8GNQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 7, 2005
Last modified:	April 3, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program