Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00551

- KKA1_ECOLX

UniProt

P00551 - KKA1_ECOLX

Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA1

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (07 Jun 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

    Catalytic activityi

    ATP + kanamycin = ADP + kanamycin 3'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei198 – 1981Proton acceptorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kanamycin kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SABIO-RKP00551.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
    Alternative name(s):
    APH(3')-I
    Short name:
    APH(3')I
    Kanamycin kinase, type I
    Neomycin-kanamycin phosphotransferase type I
    Gene namesi
    Name:aphA1
    Synonyms:nptI
    Encoded oniPlasmid Rts11 Publication
    Plasmid pIP15181 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 271271Aminoglycoside 3'-phosphotransferasePRO_0000204802Add
    BLAST

    Proteomic databases

    PRIDEiP00551.

    Interactioni

    Protein-protein interaction databases

    IntActiP00551. 2 interactions.
    MINTiMINT-6478052.

    Structurei

    3D structure databases

    ProteinModelPortaliP00551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002575. Aminoglycoside_PTrfase.
    IPR024165. Kan/Strep_kinase.
    IPR011009. Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21310. PTHR21310. 1 hit.
    PfamiPF01636. APH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00551-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP    50
    ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA 100
    IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA 150
    QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS 200
    LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK 250
    YGIDNPDMNK LQFHLMLDEF F 271
    Length:271
    Mass (Da):30,961
    Last modified:June 7, 2005 - v2
    Checksum:i3B715D9BBE8031B8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191L → M(PubMed:6270337)Curated
    Sequence conflicti19 – 191L → M1 PublicationCurated
    Sequence conflicti48 – 481D → N in CAA68507. (PubMed:2823223)Curated
    Sequence conflicti120 – 1201I → N in CAA68507. (PubMed:2823223)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti27 – 271K → R in plasmid Rts1 and strain X.
    Natural varianti77 – 771E → A in plasmid Rts1 and strain X.
    Natural varianti126 – 1261V → A in plasmid Rts1.
    Natural varianti150 – 1501A → T in plasmid Rts1.
    Natural varianti247 – 2471L → F in plasmid Rts1.
    Natural varianti249 – 2491Q → Y in plasmid Rts1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00359 Genomic DNA. Translation: CAA23656.1.
    Y00452 Genomic DNA. Translation: CAA68507.1.
    M65202 Unassigned DNA. Translation: AAA72095.1.
    AF498082 Genomic DNA. Translation: AAN59785.1.
    S54065 Genomic DNA. Translation: AAD13871.1.
    V00621 Genomic DNA. Translation: CAA23898.1.
    M10596 Genomic DNA. Translation: AAA27445.1.
    M10597 Genomic DNA. Translation: AAA27446.1.
    V00293 Genomic DNA. Translation: CAA23568.1.
    PIRiA00662. PKECT9.
    RefSeqiYP_001687822.1. NC_004429.1.
    YP_002891173.1. NC_012690.1.
    YP_002995708.1. NC_012886.1.
    YP_008864028.1. NC_022992.1.
    YP_008864695.1. NC_022996.1.
    YP_008995202.1. NC_023277.2.
    YP_008997405.1. NC_023289.2.

    Genome annotation databases

    GeneIDi17824309.
    17824444.
    18157677.
    18157927.
    5951155.
    7872406.
    8094921.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00359 Genomic DNA. Translation: CAA23656.1 .
    Y00452 Genomic DNA. Translation: CAA68507.1 .
    M65202 Unassigned DNA. Translation: AAA72095.1 .
    AF498082 Genomic DNA. Translation: AAN59785.1 .
    S54065 Genomic DNA. Translation: AAD13871.1 .
    V00621 Genomic DNA. Translation: CAA23898.1 .
    M10596 Genomic DNA. Translation: AAA27445.1 .
    M10597 Genomic DNA. Translation: AAA27446.1 .
    V00293 Genomic DNA. Translation: CAA23568.1 .
    PIRi A00662. PKECT9.
    RefSeqi YP_001687822.1. NC_004429.1.
    YP_002891173.1. NC_012690.1.
    YP_002995708.1. NC_012886.1.
    YP_008864028.1. NC_022992.1.
    YP_008864695.1. NC_022996.1.
    YP_008995202.1. NC_023277.2.
    YP_008997405.1. NC_023289.2.

    3D structure databases

    ProteinModelPortali P00551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00551. 2 interactions.
    MINTi MINT-6478052.

    Chemistry

    DrugBanki DB01172. Kanamycin.

    Proteomic databases

    PRIDEi P00551.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17824309.
    17824444.
    18157677.
    18157927.
    5951155.
    7872406.
    8094921.

    Enzyme and pathway databases

    SABIO-RK P00551.

    Family and domain databases

    InterProi IPR002575. Aminoglycoside_PTrfase.
    IPR024165. Kan/Strep_kinase.
    IPR011009. Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21310. PTHR21310. 1 hit.
    Pfami PF01636. APH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000706. Kanamycin_kin. 1 hit.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the kanamycin resistance transposon Tn903."
      Oka A., Sugisaki H., Takanami M.
      J. Mol. Biol. 147:217-226(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn903.
    2. "The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene from E. coli."
      Vakulenko S., Kalman M., Horvath B., Simoncsits A.
      Nucleic Acids Res. 15:8111-8111(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: X.
    3. "Three short fragments of Rts1 DNA are responsible for the temperature-sensitive growth phenotype (Tsg) of host bacteria."
      Mochida S., Tsuchiya H., Mori K., Kaji A.
      J. Bacteriol. 173:2600-2607(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: Rts1
    4. Press C.M., Mahaffee W.F., Roche M.M.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Overproduction of 3'-aminoglycoside phosphotransferase type I confers resistance to tobramycin in Escherichia coli."
      Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R.
      Antimicrob. Agents Chemother. 37:78-83(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
      Strain: HM69.
      Plasmid: pIP1518
    6. "Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903."
      Grindley N.D.F., Joyce C.M.
      Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
      Transposon: Tn903.
    7. "Analysis of the structure and function of the kanamycin-resistance transposon Tn903."
      Grindley N.D.F., Joyce C.M.
      Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271.
      Transposon: Tn903.
    8. "Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication."
      Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L., Jansz H.S.
      Nucleic Acids Res. 9:3335-3354(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.

    Entry informationi

    Entry nameiKKA1_ECOLX
    AccessioniPrimary (citable) accession number: P00551
    Secondary accession number(s): Q53299, Q8GNQ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme is encoded by the kanamycin resistance transposon Tn903.

    Caution

    PubMed:2823223 strain is not known and has been termed 'X' in this entry.Curated
    Was originally (PubMed:8381641) termed APH(3')IIa whereas it is APH(3')Ia.Curated

    Keywords - Technical termi

    Plasmid, Transposable element

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3