Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P00551 (KKA1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminoglycoside 3'-phosphotransferase

EC=2.7.1.95
Alternative name(s):
APH(3')-I
Short name=APH(3')I
Kanamycin kinase, type I
Neomycin-kanamycin phosphotransferase type I
Gene names
Name:aphA1
Synonyms:nptI
Encoded onPlasmid Rts1 Ref.3
Plasmid pIP1518 Ref.5
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Catalytic activity

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Miscellaneous

This enzyme is encoded by the kanamycin resistance transposon Tn903.

Sequence similarities

Belongs to the aminoglycoside phosphotransferase family.

Caution

Ref.2 strain is not known and has been termed 'X' in this entry.

Was originally (Ref.4) termed APH(3')IIa whereas it is APH(3')Ia.

Ontologies

Keywords
   Biological processAntibiotic resistance
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termPlasmid
Transposable element
Gene Ontology (GO)
   Biological_processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

kanamycin kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Aminoglycoside 3'-phosphotransferase
PRO_0000204802

Sites

Active site1981Proton acceptor By similarity

Natural variations

Natural variant271K → R in plasmid Rts1 and strain X.
Natural variant771E → A in plasmid Rts1 and strain X.
Natural variant1261V → A in plasmid Rts1.
Natural variant1501A → T in plasmid Rts1.
Natural variant2471L → F in plasmid Rts1.
Natural variant2491Q → Y in plasmid Rts1.

Experimental info

Sequence conflict191L → M Ref.1
Sequence conflict191L → M Ref.4
Sequence conflict481D → N in CAA68507. Ref.2
Sequence conflict1201I → N in CAA68507. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P00551 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 3B715D9BBE8031B8

FASTA27130,961
        10         20         30         40         50         60 
MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS 

        70         80         90        100        110        120 
VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI 

       130        140        150        160        170        180 
VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK 

       190        200        210        220        230        240 
EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS 

       250        260        270 
PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F 

« Hide

References

[1]"Nucleotide sequence of the kanamycin resistance transposon Tn903."
Oka A., Sugisaki H., Takanami M.
J. Mol. Biol. 147:217-226(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn903.
[2]"The nucleotide sequence of an aminoglycoside 3'-phosphotransferase gene from E. coli."
Vakulenko S., Kalman M., Horvath B., Simoncsits A.
Nucleic Acids Res. 15:8111-8111(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: X.
[3]"Three short fragments of Rts1 DNA are responsible for the temperature-sensitive growth phenotype (Tsg) of host bacteria."
Mochida S., Tsuchiya H., Mori K., Kaji A.
J. Bacteriol. 173:2600-2607(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Press C.M., Mahaffee W.F., Roche M.M.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Overproduction of 3'-aminoglycoside phosphotransferase type I confers resistance to tobramycin in Escherichia coli."
Menard R., Molinas C., Arthur M., Duval J., Courvalin P., Leclercq R.
Antimicrob. Agents Chemother. 37:78-83(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
Strain: HM69.
[6]"Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903."
Grindley N.D.F., Joyce C.M.
Proc. Natl. Acad. Sci. U.S.A. 77:7176-7180(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Transposon: Tn903.
[7]"Analysis of the structure and function of the kanamycin-resistance transposon Tn903."
Grindley N.D.F., Joyce C.M.
Cold Spring Harb. Symp. Quant. Biol. 45:125-133(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11 AND 184-271.
Transposon: Tn903.
[8]"Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication."
Heidekamp F., Baas P.D., van Boom J.H., Veeneman G.H., Zipursky S.L., Jansz H.S.
Nucleic Acids Res. 9:3335-3354(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-145.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00359 Genomic DNA. Translation: CAA23656.1.
Y00452 Genomic DNA. Translation: CAA68507.1.
M65202 Unassigned DNA. Translation: AAA72095.1.
AF498082 Genomic DNA. Translation: AAN59785.1.
S54065 Genomic DNA. Translation: AAD13871.1.
V00621 Genomic DNA. Translation: CAA23898.1.
M10596 Genomic DNA. Translation: AAA27445.1.
M10597 Genomic DNA. Translation: AAA27446.1.
V00293 Genomic DNA. Translation: CAA23568.1.
PIRPKECT9. A00662.
RefSeqYP_001687822.1. NC_004429.1.
YP_002891173.1. NC_012690.1.
YP_002995708.1. NC_012886.1.
YP_008864028.1. NC_022992.1.
YP_008864695.1. NC_022996.1.
YP_008995202.1. NC_023277.2.
YP_008997405.1. NC_023289.2.

3D structure databases

ProteinModelPortalP00551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP00551. 2 interactions.
MINTMINT-6478052.

Chemistry

DrugBankDB01172. Kanamycin.

Proteomic databases

PRIDEP00551.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID17824309.
17824444.
18157677.
18157927.
5951155.
7872406.
8094921.

Enzyme and pathway databases

SABIO-RKP00551.

Family and domain databases

InterProIPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR21310. PTHR21310. 1 hit.
PfamPF01636. APH. 1 hit.
[Graphical view]
PIRSFPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKKA1_ECOLX
AccessionPrimary (citable) accession number: P00551
Secondary accession number(s): Q53299, Q8GNQ0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: July 9, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families