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Protein

Aminoglycoside 3'-phosphotransferase

Gene

aphA1

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.

Miscellaneous

This enzyme is encoded by the kanamycin resistance transposon Tn903.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei198Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAntibiotic resistance
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')-I
Short name:
APH(3')I
Kanamycin kinase, type I
Neomycin-kanamycin phosphotransferase type I
Gene namesi
Name:aphA1
Synonyms:nptI
Encoded oniPlasmid Rts11 Publication
Plasmid pIP15181 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3751655.
DrugBankiDB01172. Kanamycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048021 – 271Aminoglycoside 3'-phosphotransferaseAdd BLAST271

Proteomic databases

PRIDEiP00551.

Interactioni

Protein-protein interaction databases

IntActiP00551. 2 interactors.
MINTiMINT-6478052.

Structurei

3D structure databases

ProteinModelPortaliP00551.
SMRiP00551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19272.

Family and domain databases

CDDicd05150. APH. 1 hit.
InterProiView protein in InterPro
IPR002575. Aminoglycoside_PTrfase.
IPR024165. Kan/Strep_kinase.
IPR011009. Kinase-like_dom_sf.
PfamiView protein in Pfam
PF01636. APH. 1 hit.
PIRSFiPIRSF000706. Kanamycin_kin. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P00551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP
60 70 80 90 100
ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
110 120 130 140 150
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA
160 170 180 190 200
QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
210 220 230 240 250
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK
260 270
YGIDNPDMNK LQFHLMLDEF F
Length:271
Mass (Da):30,961
Last modified:June 7, 2005 - v2
Checksum:i3B715D9BBE8031B8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19L → M (PubMed:6270337).Curated1
Sequence conflicti19L → M (Ref. 4) Curated1
Sequence conflicti48D → N in CAA68507 (PubMed:2823223).Curated1
Sequence conflicti120I → N in CAA68507 (PubMed:2823223).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti27K → R in plasmid Rts1 and strain X. 1
Natural varianti77E → A in plasmid Rts1 and strain X. 1
Natural varianti126V → A in plasmid Rts1. 1
Natural varianti150A → T in plasmid Rts1. 1
Natural varianti247L → F in plasmid Rts1. 1
Natural varianti249Q → Y in plasmid Rts1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00359 Genomic DNA. Translation: CAA23656.1.
Y00452 Genomic DNA. Translation: CAA68507.1.
M65202 Unassigned DNA. Translation: AAA72095.1.
AF498082 Genomic DNA. Translation: AAN59785.1.
S54065 Genomic DNA. Translation: AAD13871.1.
V00621 Genomic DNA. Translation: CAA23898.1.
M10596 Genomic DNA. Translation: AAA27445.1.
M10597 Genomic DNA. Translation: AAA27446.1.
V00293 Genomic DNA. Translation: CAA23568.1.
PIRiA00662. PKECT9.
RefSeqiWP_000018322.1. NZ_NLUG01000056.1.
WP_063841683.1. NG_047429.1.
WP_063842148.1. NG_047437.1.
YP_001687822.1. NC_004429.1.
YP_002891173.1. NC_012690.1.
YP_002995708.1. NC_012886.1.
YP_008864028.1. NC_022992.1.
YP_008864695.1. NC_022996.1.
YP_008995202.1. NC_023277.2.
YP_008997405.1. NC_023289.2.

Genome annotation databases

GeneIDi17824309.
17824444.
18157677.
18157927.
5951155.
7872406.
8094921.
KEGGiag:CAA23656.

Similar proteinsi

Entry informationi

Entry nameiKKA1_ECOLX
AccessioniPrimary (citable) accession number: P00551
Secondary accession number(s): Q53299, Q8GNQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: November 22, 2017
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

PubMed:2823223 strain is not known and has been termed 'X' in this entry.Curated
Was originally (Ref. 4) termed APH(3')IIa whereas it is APH(3')Ia.Curated

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families