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Protein

PTS system mannitol-specific EIICBA component

Gene

mtlA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in D-mannitol transport (PubMed:368051, PubMed:6427236, PubMed:2123863). Also able to use D-mannonic acid (PubMed:6427236).3 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L-histidine + D-mannitol 1-phosphate(Side 2).1 Publication1 Publication

Kineticsi

  1. KM=66 µM for mannitol phosphorylation1 Publication
  1. Vmax=21.7 nmol/min/mg enzyme toward mannitol1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei384Phosphocysteine intermediate; for EIIB activity2 Publications1
Sitei538Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA1 Publication1
Active sitei554Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation3 Publications1

GO - Molecular functioni

GO - Biological processi

  • mannitol transport Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MTLA-MONOMER.
ECOL316407:JW3573-MONOMER.
MetaCyc:MTLA-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.2. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system mannitol-specific EIICBA component1 Publication
Alternative name(s):
EIICBA-Mtl1 Publication
Short name:
EII-Mtl1 Publication
Including the following 3 domains:
Mannitol permease IIC component1 Publication
Alternative name(s):
PTS system mannitol-specific EIIC component1 Publication
Mannitol-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.1971 Publication1 Publication)
Alternative name(s):
PTS system mannitol-specific EIIB component1 Publication
Mannitol-specific phosphotransferase enzyme IIA component1 Publication (EC:2.7.1.1971 Publication1 Publication)
Alternative name(s):
PTS system mannitol-specific EIIA component1 Publication
Gene namesi
Name:mtlA1 Publication
Ordered Locus Names:b3599, JW3573
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10615. mtlA.

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation1 Publication1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23Cytoplasmic1 PublicationAdd BLAST23
Transmembranei24 – 45Helical1 PublicationAdd BLAST22
Topological domaini46 – 49Periplasmic1 Publication4
Transmembranei50 – 70Helical1 PublicationAdd BLAST21
Topological domaini71 – 133Cytoplasmic1 PublicationAdd BLAST63
Transmembranei134 – 155Helical1 PublicationAdd BLAST22
Topological domaini156 – 164Periplasmic1 Publication9
Transmembranei165 – 185Helical1 PublicationAdd BLAST21
Topological domaini186 – 272Cytoplasmic1 PublicationAdd BLAST87
Transmembranei273 – 292Helical1 PublicationAdd BLAST20
Topological domaini293 – 312Periplasmic1 PublicationAdd BLAST20
Transmembranei313 – 334Helical1 PublicationAdd BLAST22
Topological domaini335 – 637Cytoplasmic1 PublicationAdd BLAST303

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001866141 – 637PTS system mannitol-specific EIICBA componentAdd BLAST637

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384Phosphocysteine; by EIIAPROSITE-ProRule annotation3 Publications1 Publication1
Modified residuei554Phosphohistidine; by HPr4 Publications1 Publication1

Post-translational modificationi

An intramolecular phosphotransfer takes places between His-554 and Cys-384.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP00550.
PaxDbiP00550.
PRIDEiP00550.

PTM databases

iPTMnetiP00550.

Expressioni

Inductioni

Induced by mannitol. Repressed by MltR.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260741. 9 interactors.
DIPiDIP-10267N.
IntActiP00550. 2 interactors.
MINTiMINT-245984.
STRINGi511145.b3599.

Structurei

Secondary structure

1637
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi379 – 382Combined sources4
Beta strandi385 – 388Combined sources4
Helixi389 – 404Combined sources16
Beta strandi410 – 414Combined sources5
Beta strandi425 – 430Combined sources6
Helixi431 – 440Combined sources10
Beta strandi444 – 450Combined sources7
Helixi455 – 470Combined sources16
Helixi498 – 500Combined sources3
Helixi510 – 523Combined sources14
Helixi530 – 541Combined sources12
Beta strandi545 – 547Combined sources3
Helixi557 – 562Combined sources6
Beta strandi563 – 565Combined sources3
Beta strandi567 – 578Combined sources12
Beta strandi580 – 582Combined sources3
Beta strandi586 – 594Combined sources9
Turni597 – 599Combined sources3
Helixi600 – 610Combined sources11
Helixi614 – 622Combined sources9
Helixi626 – 632Combined sources7
Turni633 – 635Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A3AX-ray1.80A/B/C/D491-637[»]
1J6TNMR-A491-637[»]
1VKRNMR-A367-489[»]
1VRVNMR-A375-475[»]
2FEWNMR-A491-637[»]
B375-475[»]
ProteinModelPortaliP00550.
SMRiP00550.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00550.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 341PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST330
Domaini378 – 473PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST96
Domaini494 – 636PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST143

Domaini

The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
COG4668. LUCA.
HOGENOMiHOG000252814.
InParanoidiP00550.
KOiK02798.
K02799.
K02800.
OMAiGWAIKRF.
PhylomeDBiP00550.

Family and domain databases

CDDicd05567. PTS_IIB_mannitol. 1 hit.
Gene3Di3.40.50.10370. 1 hit.
3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDIKIKVQ SFGRFLSNMV MPNIGAFIAW GIITALFIPT GWLPNETLAK
60 70 80 90 100
LVGPMITYLL PLLIGYTGGK LVGGERGGVV GAITTMGVIV GADMPMFLGS
110 120 130 140 150
MIAGPLGGWC IKHFDRWVDG KIKSGFEMLV NNFSAGIIGM ILAILAFLGI
160 170 180 190 200
GPIVEALSKM LAAGVNFMVV HDMLPLASIF VEPAKILFLN NAINHGIFSP
210 220 230 240 250
LGIQQSHELG KSIFFLIEAN PGPGMGVLLA YMFFGRGSAK QSAGGAAIIH
260 270 280 290 300
FLGGIHEIYF PYVLMNPRLI LAVILGGMTG VFTLTILGGG LVSPASPGSI
310 320 330 340 350
LAVLAMTPKG AYFANIAGVC AAMAVSFVVS AILLKTSKVK EEDDIEAATR
360 370 380 390 400
RMQDMKAESK GASPLSAGDV TNDLSHVRKI IVACDAGMGS SAMGAGVLRK
410 420 430 440 450
KIQDAGLSQI SVTNSAINNL PPDVDLVITH RDLTERAMRQ VPQAQHISLT
460 470 480 490 500
NFLDSGLYTS LTERLVAAQR HTANEEKVKD SLKDSFDDSS ANLFKLGAEN
510 520 530 540 550
IFLGRKAATK EEAIRFAGEQ LVKGGYVEPE YVQAMLDREK LTPTYLGESI
560 570 580 590 600
AVPHGTVEAK DRVLKTGVVF CQYPEGVRFG EEEDDIARLV IGIAARNNEH
610 620 630
IQVITSLTNA LDDESVIERL AHTTSVDEVL ELLAGRK
Length:637
Mass (Da):67,972
Last modified:July 21, 1986 - v1
Checksum:iA992992D534BF98D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01503 Genomic DNA. Translation: CAA24748.1.
U00039 Genomic DNA. Translation: AAB18576.1.
U00096 Genomic DNA. Translation: AAC76623.1.
AP009048 Genomic DNA. Translation: BAE77694.1.
X06794 Genomic DNA. Translation: CAA29953.1.
PIRiA00661. WQEC2M.
RefSeqiNP_418056.1. NC_000913.3.
WP_000093247.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC76623; AAC76623; b3599.
BAE77694; BAE77694; BAE77694.
GeneIDi948118.
KEGGiecj:JW3573.
eco:b3599.
PATRICi32122681. VBIEscCol129921_3717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01503 Genomic DNA. Translation: CAA24748.1.
U00039 Genomic DNA. Translation: AAB18576.1.
U00096 Genomic DNA. Translation: AAC76623.1.
AP009048 Genomic DNA. Translation: BAE77694.1.
X06794 Genomic DNA. Translation: CAA29953.1.
PIRiA00661. WQEC2M.
RefSeqiNP_418056.1. NC_000913.3.
WP_000093247.1. NZ_CP014272.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A3AX-ray1.80A/B/C/D491-637[»]
1J6TNMR-A491-637[»]
1VKRNMR-A367-489[»]
1VRVNMR-A375-475[»]
2FEWNMR-A491-637[»]
B375-475[»]
ProteinModelPortaliP00550.
SMRiP00550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260741. 9 interactors.
DIPiDIP-10267N.
IntActiP00550. 2 interactors.
MINTiMINT-245984.
STRINGi511145.b3599.

Protein family/group databases

TCDBi4.A.2.1.2. the pts fructose-mannitol (fru) family.

PTM databases

iPTMnetiP00550.

Proteomic databases

EPDiP00550.
PaxDbiP00550.
PRIDEiP00550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76623; AAC76623; b3599.
BAE77694; BAE77694; BAE77694.
GeneIDi948118.
KEGGiecj:JW3573.
eco:b3599.
PATRICi32122681. VBIEscCol129921_3717.

Organism-specific databases

EchoBASEiEB0610.
EcoGeneiEG10615. mtlA.

Phylogenomic databases

eggNOGiENOG4105CTS. Bacteria.
COG2213. LUCA.
COG4668. LUCA.
HOGENOMiHOG000252814.
InParanoidiP00550.
KOiK02798.
K02799.
K02800.
OMAiGWAIKRF.
PhylomeDBiP00550.

Enzyme and pathway databases

BioCyciEcoCyc:MTLA-MONOMER.
ECOL316407:JW3573-MONOMER.
MetaCyc:MTLA-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP00550.
PROiP00550.

Family and domain databases

CDDicd05567. PTS_IIB_mannitol. 1 hit.
Gene3Di3.40.50.10370. 1 hit.
3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR029503. PTS_EIIB_mannitol.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00851. mtlA. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTM3C_ECOLI
AccessioniPrimary (citable) accession number: P00550
Secondary accession number(s): Q2M7R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 177 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.