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P00549

- KPYK1_YEAST

UniProt

P00549 - KPYK1_YEAST

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Protein
Pyruvate kinase 1
Gene
CDC19, PYK1, YAL038W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Enzyme regulationi

The activity is regulated by glucose levels. Activated by fructose-1,6-bisphosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491Substrate By similarity
Metal bindingi51 – 511Potassium By similarity
Metal bindingi53 – 531Potassium By similarity
Metal bindingi84 – 841Potassium By similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygen By similarity
Sitei240 – 2401Transition state stabilizer By similarity
Metal bindingi242 – 2421Magnesium Reviewed prediction
Binding sitei265 – 2651Substrate; via amide nitrogen By similarity
Metal bindingi266 – 2661Magnesium By similarity
Binding sitei266 – 2661Substrate; via amide nitrogen By similarity
Binding sitei298 – 2981Substrate By similarity
Binding sitei337 – 3371ADP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: SGD
  2. pyruvate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciYEAST:YAL038W-MONOMER.
SABIO-RKP00549.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 1 (EC:2.7.1.40)
Short name:
PK 1
Alternative name(s):
cell division cycle protein 19
Gene namesi
Name:CDC19
Synonyms:PYK1
Ordered Locus Names:YAL038W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL038w.
SGDiS000000036. CDC19.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 500499Pyruvate kinase 1
PRO_0000112121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei9 – 91Phosphoserine2 Publications
Modified residuei16 – 161Phosphoserine2 Publications
Modified residuei31 – 311Phosphothreonine1 Publication
Modified residuei70 – 701Phosphoserine1 Publication
Modified residuei184 – 1841Phosphothreonine1 Publication
Modified residuei213 – 2131Phosphoserine1 Publication
Modified residuei316 – 3161Phosphoserine1 Publication
Modified residuei450 – 4501Phosphoserine1 Publication
Modified residuei478 – 4781Phosphothreonine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00549.
PaxDbiP00549.
PeptideAtlasiP00549.

2D gel databases

COMPLUYEAST-2DPAGEP00549.

Expressioni

Gene expression databases

GenevestigatoriP00549.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi31727. 144 interactions.
DIPiDIP-4124N.
IntActiP00549. 182 interactions.
MINTiMINT-565419.
STRINGi4932.YAL038W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 86
Beta strandi21 – 266
Helixi29 – 313
Helixi34 – 4310
Beta strandi47 – 493
Helixi57 – 7317
Beta strandi82 – 843
Beta strandi96 – 994
Beta strandi108 – 1125
Turni116 – 1205
Beta strandi126 – 1294
Helixi133 – 1364
Beta strandi142 – 1454
Turni146 – 1494
Beta strandi150 – 1534
Turni159 – 1613
Beta strandi163 – 1675
Beta strandi178 – 1803
Helixi193 – 20513
Beta strandi208 – 2125
Helixi218 – 23215
Beta strandi235 – 2417
Helixi245 – 2484
Helixi250 – 2567
Beta strandi260 – 2623
Helixi264 – 2707
Helixi273 – 2753
Helixi276 – 29015
Beta strandi294 – 2963
Helixi302 – 3054
Helixi312 – 32413
Beta strandi327 – 3293
Turni333 – 3375
Helixi341 – 35515
Beta strandi357 – 3593
Helixi361 – 3688
Helixi378 – 39316
Beta strandi398 – 4014
Beta strandi403 – 4053
Helixi406 – 4138
Beta strandi420 – 4256
Helixi429 – 4324
Helixi433 – 4353
Beta strandi439 – 4435
Turni452 – 4543
Helixi455 – 46915
Beta strandi478 – 4836
Turni487 – 4893
Beta strandi494 – 4996

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3WX-ray3.00A/B1-500[»]
1A3XX-ray3.00A/B1-500[»]
ProteinModelPortaliP00549.
SMRiP00549. Positions 2-500.

Miscellaneous databases

EvolutionaryTraceiP00549.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
KOiK00873.
OMAiHADHQRS.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00549-1 [UniParc]FASTAAdd to Basket

« Hide

MSRLERLTSL NVVAGSDLRR TSIIGTIGPK TNNPETLVAL RKAGLNIVRM    50
NFSHGSYEYH KSVIDNARKS EELYPGRPLA IALDTKGPEI RTGTTTNDVD 100
YPIPPNHEMI FTTDDKYAKA CDDKIMYVDY KNITKVISAG RIIYVDDGVL 150
SFQVLEVVDD KTLKVKALNA GKICSHKGVN LPGTDVDLPA LSEKDKEDLR 200
FGVKNGVHMV FASFIRTAND VLTIREVLGE QGKDVKIIVK IENQQGVNNF 250
DEILKVTDGV MVARGDLGIE IPAPEVLAVQ KKLIAKSNLA GKPVICATQM 300
LESMTYNPRP TRAEVSDVGN AILDGADCVM LSGETAKGNY PINAVTTMAE 350
TAVIAEQAIA YLPNYDDMRN CTPKPTSTTE TVAASAVAAV FEQKAKAIIV 400
LSTSGTTPRL VSKYRPNCPI ILVTRCPRAA RFSHLYRGVF PFVFEKEPVS 450
DWTDDVEARI NFGIEKAKEF GILKKGDTYV SIQGFKAGAG HSNTLQVSTV 500
Length:500
Mass (Da):54,545
Last modified:July 1, 1989 - v2
Checksum:i78D753FC410C5820
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti382 – 3865VAASA → SLPR in CAA24631. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01321 Genomic DNA. Translation: CAA24631.1.
X14400 Genomic DNA. Translation: CAA32573.1.
AY949862 Genomic DNA. Translation: AAY27264.1.
AY949863 Genomic DNA. Translation: AAY27265.1.
AY949864 Genomic DNA. Translation: AAY27266.1.
AY949865 Genomic DNA. Translation: AAY27267.1.
AY949866 Genomic DNA. Translation: AAY27268.1.
AY949867 Genomic DNA. Translation: AAY27269.1.
AY949868 Genomic DNA. Translation: AAY27270.1.
AY949869 Genomic DNA. Translation: AAY27271.1.
AY949870 Genomic DNA. Translation: AAY27272.1.
AY949871 Genomic DNA. Translation: AAY27273.1.
AY949872 Genomic DNA. Translation: AAY27274.1.
AY949873 Genomic DNA. Translation: AAY27275.1.
AY949874 Genomic DNA. Translation: AAY27276.1.
AY949875 Genomic DNA. Translation: AAY27277.1.
AY949876 Genomic DNA. Translation: AAY27278.1.
AY949877 Genomic DNA. Translation: AAY27279.1.
AY949878 Genomic DNA. Translation: AAY27280.1.
AY949879 Genomic DNA. Translation: AAY27281.1.
AY949880 Genomic DNA. Translation: AAY27282.1.
AY949881 Genomic DNA. Translation: AAY27283.1.
AY949882 Genomic DNA. Translation: AAY27284.1.
AY949883 Genomic DNA. Translation: AAY27285.1.
AY949884 Genomic DNA. Translation: AAY27286.1.
AY949885 Genomic DNA. Translation: AAY27287.1.
AY949886 Genomic DNA. Translation: AAY27288.1.
AY949887 Genomic DNA. Translation: AAY27289.1.
AY949888 Genomic DNA. Translation: AAY27290.1.
AY949889 Genomic DNA. Translation: AAY27291.1.
AY949890 Genomic DNA. Translation: AAY27292.1.
U12980 Genomic DNA. Translation: AAC04993.1.
AY693107 Genomic DNA. Translation: AAT93126.1.
BK006935 Genomic DNA. Translation: DAA06948.1.
PIRiS05764. KIBYP.
RefSeqiNP_009362.1. NM_001178183.1.

Genome annotation databases

EnsemblFungiiYAL038W; YAL038W; YAL038W.
GeneIDi851193.
KEGGisce:YAL038W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01321 Genomic DNA. Translation: CAA24631.1 .
X14400 Genomic DNA. Translation: CAA32573.1 .
AY949862 Genomic DNA. Translation: AAY27264.1 .
AY949863 Genomic DNA. Translation: AAY27265.1 .
AY949864 Genomic DNA. Translation: AAY27266.1 .
AY949865 Genomic DNA. Translation: AAY27267.1 .
AY949866 Genomic DNA. Translation: AAY27268.1 .
AY949867 Genomic DNA. Translation: AAY27269.1 .
AY949868 Genomic DNA. Translation: AAY27270.1 .
AY949869 Genomic DNA. Translation: AAY27271.1 .
AY949870 Genomic DNA. Translation: AAY27272.1 .
AY949871 Genomic DNA. Translation: AAY27273.1 .
AY949872 Genomic DNA. Translation: AAY27274.1 .
AY949873 Genomic DNA. Translation: AAY27275.1 .
AY949874 Genomic DNA. Translation: AAY27276.1 .
AY949875 Genomic DNA. Translation: AAY27277.1 .
AY949876 Genomic DNA. Translation: AAY27278.1 .
AY949877 Genomic DNA. Translation: AAY27279.1 .
AY949878 Genomic DNA. Translation: AAY27280.1 .
AY949879 Genomic DNA. Translation: AAY27281.1 .
AY949880 Genomic DNA. Translation: AAY27282.1 .
AY949881 Genomic DNA. Translation: AAY27283.1 .
AY949882 Genomic DNA. Translation: AAY27284.1 .
AY949883 Genomic DNA. Translation: AAY27285.1 .
AY949884 Genomic DNA. Translation: AAY27286.1 .
AY949885 Genomic DNA. Translation: AAY27287.1 .
AY949886 Genomic DNA. Translation: AAY27288.1 .
AY949887 Genomic DNA. Translation: AAY27289.1 .
AY949888 Genomic DNA. Translation: AAY27290.1 .
AY949889 Genomic DNA. Translation: AAY27291.1 .
AY949890 Genomic DNA. Translation: AAY27292.1 .
U12980 Genomic DNA. Translation: AAC04993.1 .
AY693107 Genomic DNA. Translation: AAT93126.1 .
BK006935 Genomic DNA. Translation: DAA06948.1 .
PIRi S05764. KIBYP.
RefSeqi NP_009362.1. NM_001178183.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A3W X-ray 3.00 A/B 1-500 [» ]
1A3X X-ray 3.00 A/B 1-500 [» ]
ProteinModelPortali P00549.
SMRi P00549. Positions 2-500.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31727. 144 interactions.
DIPi DIP-4124N.
IntActi P00549. 182 interactions.
MINTi MINT-565419.
STRINGi 4932.YAL038W.

2D gel databases

COMPLUYEAST-2DPAGE P00549.

Proteomic databases

MaxQBi P00549.
PaxDbi P00549.
PeptideAtlasi P00549.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL038W ; YAL038W ; YAL038W .
GeneIDi 851193.
KEGGi sce:YAL038W.

Organism-specific databases

CYGDi YAL038w.
SGDi S000000036. CDC19.

Phylogenomic databases

eggNOGi COG0469.
GeneTreei ENSGT00390000008859.
HOGENOMi HOG000021559.
KOi K00873.
OMAi HADHQRS.
OrthoDBi EOG7M6DJC.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci YEAST:YAL038W-MONOMER.
SABIO-RK P00549.

Miscellaneous databases

EvolutionaryTracei P00549.
NextBioi 968037.

Gene expression databases

Genevestigatori P00549.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast pyruvate kinase gene does not contain a string of non-preferred codons: revised nucleotide sequence."
    McNally T., Purvis I.J., Fothergill-Gilmore L.A., Brown A.L.P.
    FEBS Lett. 247:312-316(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The isolation, characterization, and sequence of the pyruvate kinase gene of Saccharomyces cerevisiae."
    Burke R.L., Tekamp-Olson P., Najarian R.
    J. Biol. Chem. 258:2193-2201(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Population structure and gene evolution in Saccharomyces cerevisiae."
    Aa E., Townsend J.P., Adams R.I., Nielsen K.M., Taylor J.W.
    FEMS Yeast Res. 6:702-715(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 76625 / YPH499, Ba194, Bb32, Fy93, M1-2A, M2-8, M5-7A, M5-7B, M7-8D, MMR2-1, MMR2-3, MMR2-5, MMW1-12, MMW1-15, MMW1-15h2, MMW1-2, MMW1-2h2, ORM1-1, Sgu52E, Sgu52F, YPS396, YPS400, YPS598, YPS600, YPS602, YPS604, YPS606, YPS608 and YPS610.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND THR-478, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-16; THR-31; SER-70; THR-184; SER-316; SER-450 AND THR-478, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "The allosteric regulation of pyruvate kinase by fructose-1,6-bisphosphate."
    Jurica M.S., Mesecar A., Heath P.J., Shi W., Nowak T., Stoddard B.L.
    Structure 6:195-210(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Entry informationi

Entry nameiKPYK1_YEAST
AccessioniPrimary (citable) accession number: P00549
Secondary accession number(s): D6VPH8, Q2VQG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: June 11, 2014
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 291000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi