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P00548

- KPYM_CHICK

UniProt

P00548 - KPYM_CHICK

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Protein
Pyruvate kinase PKM
Gene
PKM
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a key role in glycolysis By similarity.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721Substrate By similarity
Metal bindingi74 – 741Potassium By similarity
Metal bindingi76 – 761Potassium By similarity
Metal bindingi112 – 1121Potassium By similarity
Metal bindingi113 – 1131Potassium; via carbonyl oxygen By similarity
Sitei269 – 2691Transition state stabilizer By similarity
Metal bindingi271 – 2711Magnesium By similarity
Binding sitei294 – 2941Substrate; via amide nitrogen By similarity
Metal bindingi295 – 2951Magnesium By similarity
Binding sitei295 – 2951Substrate; via amide nitrogen By similarity
Binding sitei327 – 3271Substrate By similarity
Binding sitei481 – 4811Allosteric activator By similarity
Binding sitei488 – 4881Allosteric activator By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. glycolytic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_115767. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKM (EC:2.7.1.40)
Gene namesi
Name:PKM
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 10

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 530529Pyruvate kinase PKM
PRO_0000112097Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00548.
PRIDEiP00548.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi676705. 2 interactions.
IntActiP00548. 1 interaction.
STRINGi9031.ENSGALP00000034108.

Structurei

3D structure databases

ProteinModelPortaliP00548.
SMRiP00548. Positions 12-530.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni431 – 4366Allosteric activator binding By similarity
Regioni515 – 5206Allosteric activator binding By similarity

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
HOVERGENiHBG000941.
KOiK00873.
PhylomeDBiP00548.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00548-1 [UniParc]FASTAAdd to Basket

« Hide

MSKHHDAGTA FIQTQQLHAA MADTFLEHMC RLDIDSEPTI ARNTGIICTI    50
GPASRSVDKL KEMIKSGMNV ARLNFSHGTH EYHEGTIKNV REATESFASD 100
PITYRPVAIA LDTKGPEIRT GLIKGSGTAE VELKKGAALK VTLDNAFMEN 150
CDENVLWVDY KNLIKVIDVG SKIYVDDGLI SLLVKEKGKD FVMTEVENGG 200
MLGSKKGVNL PGAAVDLPAV SEKDIQDLKF GVEQNVDMVF ASFIRKAADV 250
HAVRKVLGEK GKHIKIISKI ENHEGVRRFD EIMEASDGIM VARGDLGIEI 300
PAEKVFLAQK MMIGRCNRAG KPIICATQML ESMIKKPRPT RAEGSDVANA 350
VLDGADCIML SGETAKGDYP LEAVRMQHAI AREAEAAMFH RQQFEEILRH 400
SVHHREPADA MAAGAVEASF KCLAAALIVM TESGRSAHLV SRYRPRAPII 450
AVTRNDQTAR QAHLYRGVFP VLCKQPAHDA WAEDVDLRVN LGMNVGKARG 500
FFKTGDLVIV LTGWRPGSGY TNTMRVVPVP 530
Length:530
Mass (Da):58,015
Last modified:January 23, 2007 - v2
Checksum:i8B0DF09FD82EB72F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00903 mRNA. Translation: AAA49021.1.
M18793
, M10619, M18788, M18789, M18790, M18791, M18792 Genomic DNA. Translation: AAA49020.1.
PIRiI50408. KICHPM.
RefSeqiNP_990800.1. NM_205469.1.
UniGeneiGga.4299.

Genome annotation databases

EnsembliENSGALT00000003100; ENSGALP00000003096; ENSGALG00000001992.
GeneIDi396456.
KEGGigga:396456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J00903 mRNA. Translation: AAA49021.1 .
M18793
, M10619 , M18788 , M18789 , M18790 , M18791 , M18792 Genomic DNA. Translation: AAA49020.1 .
PIRi I50408. KICHPM.
RefSeqi NP_990800.1. NM_205469.1.
UniGenei Gga.4299.

3D structure databases

ProteinModelPortali P00548.
SMRi P00548. Positions 12-530.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 676705. 2 interactions.
IntActi P00548. 1 interaction.
STRINGi 9031.ENSGALP00000034108.

Proteomic databases

PaxDbi P00548.
PRIDEi P00548.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000003100 ; ENSGALP00000003096 ; ENSGALG00000001992 .
GeneIDi 396456.
KEGGi gga:396456.

Organism-specific databases

CTDi 396456.

Phylogenomic databases

eggNOGi COG0469.
GeneTreei ENSGT00390000008859.
HOGENOMi HOG000021559.
HOVERGENi HBG000941.
KOi K00873.
PhylomeDBi P00548.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_115767. Glycolysis.

Miscellaneous databases

NextBioi 20816497.
PROi P00548.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of chicken muscle pyruvate kinase mRNA."
    Lonberg N., Gilbert W.
    Proc. Natl. Acad. Sci. U.S.A. 80:3661-3665(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Intron/exon structure of the chicken pyruvate kinase gene."
    Lonberg N., Gilbert W.
    Cell 40:81-90(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYM_CHICK
AccessioniPrimary (citable) accession number: P00548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This activity is regulated by glucose levels.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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