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Protein

Pyruvate kinase PKM

Gene

PKM

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in glycolysis.By similarity

Miscellaneous

This activity is regulated by glucose levels.

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  2. Phosphoglycerate kinase (PGK)
  3. no protein annotated in this organism
  4. Gamma-enolase (ENO2), Alpha-enolase (ENO1), Beta-enolase (ENO3)
  5. Pyruvate kinase (PKM), Pyruvate kinase PKM (PKM), Pyruvate kinase (PKM)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Metal bindingi74PotassiumBy similarity1
Metal bindingi76PotassiumBy similarity1
Metal bindingi112PotassiumBy similarity1
Metal bindingi113Potassium; via carbonyl oxygenBy similarity1
Sitei269Transition state stabilizerBy similarity1
Metal bindingi271MagnesiumBy similarity1
Binding sitei294Substrate; via amide nitrogenBy similarity1
Metal bindingi295MagnesiumBy similarity1
Binding sitei295Substrate; via amide nitrogenBy similarity1
Binding sitei327SubstrateBy similarity1
Binding sitei481Allosteric activatorBy similarity1
Binding sitei488Allosteric activatorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiR-GGA-352882. Glycolysis.
R-GGA-6798695. Neutrophil degranulation.
R-GGA-70171. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKM (EC:2.7.1.40)
Gene namesi
Name:PKM
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001120972 – 530Pyruvate kinase PKMAdd BLAST529

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00548.
PRIDEiP00548.

Expressioni

Gene expression databases

BgeeiENSGALG00000001992.
ExpressionAtlasiP00548. baseline.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi676705. 2 interactors.
IntActiP00548. 1 interactor.
STRINGi9031.ENSGALP00000034108.

Structurei

3D structure databases

ProteinModelPortaliP00548.
SMRiP00548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni431 – 436Allosteric activator bindingBy similarity6
Regioni515 – 520Allosteric activator bindingBy similarity6

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
HOVERGENiHBG000941.
InParanoidiP00548.
KOiK00873.
OMAiKHEAIEQ.
OrthoDBiEOG091G0597.
PhylomeDBiP00548.

Family and domain databases

CDDicd00288. Pyruvate_Kinase. 1 hit.
Gene3Di3.40.1380.20. 1 hit.
InterProiView protein in InterPro
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiView protein in Pfam
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiView protein in PROSITE
PS00110. PYRUVATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKHHDAGTA FIQTQQLHAA MADTFLEHMC RLDIDSEPTI ARNTGIICTI
60 70 80 90 100
GPASRSVDKL KEMIKSGMNV ARLNFSHGTH EYHEGTIKNV REATESFASD
110 120 130 140 150
PITYRPVAIA LDTKGPEIRT GLIKGSGTAE VELKKGAALK VTLDNAFMEN
160 170 180 190 200
CDENVLWVDY KNLIKVIDVG SKIYVDDGLI SLLVKEKGKD FVMTEVENGG
210 220 230 240 250
MLGSKKGVNL PGAAVDLPAV SEKDIQDLKF GVEQNVDMVF ASFIRKAADV
260 270 280 290 300
HAVRKVLGEK GKHIKIISKI ENHEGVRRFD EIMEASDGIM VARGDLGIEI
310 320 330 340 350
PAEKVFLAQK MMIGRCNRAG KPIICATQML ESMIKKPRPT RAEGSDVANA
360 370 380 390 400
VLDGADCIML SGETAKGDYP LEAVRMQHAI AREAEAAMFH RQQFEEILRH
410 420 430 440 450
SVHHREPADA MAAGAVEASF KCLAAALIVM TESGRSAHLV SRYRPRAPII
460 470 480 490 500
AVTRNDQTAR QAHLYRGVFP VLCKQPAHDA WAEDVDLRVN LGMNVGKARG
510 520 530
FFKTGDLVIV LTGWRPGSGY TNTMRVVPVP
Length:530
Mass (Da):58,015
Last modified:January 23, 2007 - v2
Checksum:i8B0DF09FD82EB72F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00903 mRNA. Translation: AAA49021.1.
M18793
, M10619, M18788, M18789, M18790, M18791, M18792 Genomic DNA. Translation: AAA49020.1.
PIRiI50408. KICHPM.
RefSeqiNP_990800.1. NM_205469.1.
UniGeneiGga.4299.

Genome annotation databases

EnsembliENSGALT00000003100; ENSGALP00000003096; ENSGALG00000001992.
GeneIDi396456.
KEGGigga:396456.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKPYM_CHICK
AccessioniPrimary (citable) accession number: P00548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families