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P00548

- KPYM_CHICK

UniProt

P00548 - KPYM_CHICK

Protein

Pyruvate kinase PKM

Gene

PKM

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays a key role in glycolysis.By similarity

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Allosterically activated by fructose 1,6-bisphosphate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei72 – 721SubstrateBy similarity
    Metal bindingi74 – 741PotassiumBy similarity
    Metal bindingi76 – 761PotassiumBy similarity
    Metal bindingi112 – 1121PotassiumBy similarity
    Metal bindingi113 – 1131Potassium; via carbonyl oxygenBy similarity
    Sitei269 – 2691Transition state stabilizerBy similarity
    Metal bindingi271 – 2711MagnesiumBy similarity
    Binding sitei294 – 2941Substrate; via amide nitrogenBy similarity
    Metal bindingi295 – 2951MagnesiumBy similarity
    Binding sitei295 – 2951Substrate; via amide nitrogenBy similarity
    Binding sitei327 – 3271SubstrateBy similarity
    Binding sitei481 – 4811Allosteric activatorBy similarity
    Binding sitei488 – 4881Allosteric activatorBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. glycolytic process Source: Reactome
    3. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    ReactomeiREACT_115767. Glycolysis.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase PKM (EC:2.7.1.40)
    Gene namesi
    Name:PKM
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 10

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 530529Pyruvate kinase PKMPRO_0000112097Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP00548.
    PRIDEiP00548.

    Interactioni

    Subunit structurei

    Homotetramer.

    Protein-protein interaction databases

    BioGridi676705. 2 interactions.
    IntActiP00548. 1 interaction.
    STRINGi9031.ENSGALP00000034108.

    Structurei

    3D structure databases

    ProteinModelPortaliP00548.
    SMRiP00548. Positions 12-530.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni431 – 4366Allosteric activator bindingBy similarity
    Regioni515 – 5206Allosteric activator bindingBy similarity

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    GeneTreeiENSGT00390000008859.
    HOGENOMiHOG000021559.
    HOVERGENiHBG000941.
    KOiK00873.
    PhylomeDBiP00548.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00548-1 [UniParc]FASTAAdd to Basket

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    MSKHHDAGTA FIQTQQLHAA MADTFLEHMC RLDIDSEPTI ARNTGIICTI    50
    GPASRSVDKL KEMIKSGMNV ARLNFSHGTH EYHEGTIKNV REATESFASD 100
    PITYRPVAIA LDTKGPEIRT GLIKGSGTAE VELKKGAALK VTLDNAFMEN 150
    CDENVLWVDY KNLIKVIDVG SKIYVDDGLI SLLVKEKGKD FVMTEVENGG 200
    MLGSKKGVNL PGAAVDLPAV SEKDIQDLKF GVEQNVDMVF ASFIRKAADV 250
    HAVRKVLGEK GKHIKIISKI ENHEGVRRFD EIMEASDGIM VARGDLGIEI 300
    PAEKVFLAQK MMIGRCNRAG KPIICATQML ESMIKKPRPT RAEGSDVANA 350
    VLDGADCIML SGETAKGDYP LEAVRMQHAI AREAEAAMFH RQQFEEILRH 400
    SVHHREPADA MAAGAVEASF KCLAAALIVM TESGRSAHLV SRYRPRAPII 450
    AVTRNDQTAR QAHLYRGVFP VLCKQPAHDA WAEDVDLRVN LGMNVGKARG 500
    FFKTGDLVIV LTGWRPGSGY TNTMRVVPVP 530
    Length:530
    Mass (Da):58,015
    Last modified:January 23, 2007 - v2
    Checksum:i8B0DF09FD82EB72F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00903 mRNA. Translation: AAA49021.1.
    M18793
    , M10619, M18788, M18789, M18790, M18791, M18792 Genomic DNA. Translation: AAA49020.1.
    PIRiI50408. KICHPM.
    RefSeqiNP_990800.1. NM_205469.1.
    UniGeneiGga.4299.

    Genome annotation databases

    EnsembliENSGALT00000003100; ENSGALP00000003096; ENSGALG00000001992.
    GeneIDi396456.
    KEGGigga:396456.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00903 mRNA. Translation: AAA49021.1 .
    M18793
    , M10619 , M18788 , M18789 , M18790 , M18791 , M18792 Genomic DNA. Translation: AAA49020.1 .
    PIRi I50408. KICHPM.
    RefSeqi NP_990800.1. NM_205469.1.
    UniGenei Gga.4299.

    3D structure databases

    ProteinModelPortali P00548.
    SMRi P00548. Positions 12-530.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 676705. 2 interactions.
    IntActi P00548. 1 interaction.
    STRINGi 9031.ENSGALP00000034108.

    Proteomic databases

    PaxDbi P00548.
    PRIDEi P00548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000003100 ; ENSGALP00000003096 ; ENSGALG00000001992 .
    GeneIDi 396456.
    KEGGi gga:396456.

    Organism-specific databases

    CTDi 396456.

    Phylogenomic databases

    eggNOGi COG0469.
    GeneTreei ENSGT00390000008859.
    HOGENOMi HOG000021559.
    HOVERGENi HBG000941.
    KOi K00873.
    PhylomeDBi P00548.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    Reactomei REACT_115767. Glycolysis.

    Miscellaneous databases

    NextBioi 20816497.
    PROi P00548.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of chicken muscle pyruvate kinase mRNA."
      Lonberg N., Gilbert W.
      Proc. Natl. Acad. Sci. U.S.A. 80:3661-3665(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Intron/exon structure of the chicken pyruvate kinase gene."
      Lonberg N., Gilbert W.
      Cell 40:81-90(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiKPYM_CHICK
    AccessioniPrimary (citable) accession number: P00548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This activity is regulated by glucose levels.

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3