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P00548

- KPYM_CHICK

UniProt

P00548 - KPYM_CHICK

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Protein

Pyruvate kinase PKM

Gene

PKM

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Plays a key role in glycolysis.By similarity

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721SubstrateBy similarity
Metal bindingi74 – 741PotassiumBy similarity
Metal bindingi76 – 761PotassiumBy similarity
Metal bindingi112 – 1121PotassiumBy similarity
Metal bindingi113 – 1131Potassium; via carbonyl oxygenBy similarity
Sitei269 – 2691Transition state stabilizerBy similarity
Metal bindingi271 – 2711MagnesiumBy similarity
Binding sitei294 – 2941Substrate; via amide nitrogenBy similarity
Metal bindingi295 – 2951MagnesiumBy similarity
Binding sitei295 – 2951Substrate; via amide nitrogenBy similarity
Binding sitei327 – 3271SubstrateBy similarity
Binding sitei481 – 4811Allosteric activatorBy similarity
Binding sitei488 – 4881Allosteric activatorBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. glycolytic process Source: Reactome
  3. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiREACT_115767. Glycolysis.
REACT_247441. Glycolysis.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase PKM (EC:2.7.1.40)
Gene namesi
Name:PKM
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome 10

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 530529Pyruvate kinase PKMPRO_0000112097Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00548.
PRIDEiP00548.

Expressioni

Gene expression databases

ExpressionAtlasiP00548. baseline.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi676705. 2 interactions.
IntActiP00548. 1 interaction.
STRINGi9031.ENSGALP00000034108.

Structurei

3D structure databases

ProteinModelPortaliP00548.
SMRiP00548. Positions 12-530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni431 – 4366Allosteric activator bindingBy similarity
Regioni515 – 5206Allosteric activator bindingBy similarity

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
GeneTreeiENSGT00390000008859.
HOGENOMiHOG000021559.
HOVERGENiHBG000941.
InParanoidiP00548.
KOiK00873.
PhylomeDBiP00548.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00548-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKHHDAGTA FIQTQQLHAA MADTFLEHMC RLDIDSEPTI ARNTGIICTI
60 70 80 90 100
GPASRSVDKL KEMIKSGMNV ARLNFSHGTH EYHEGTIKNV REATESFASD
110 120 130 140 150
PITYRPVAIA LDTKGPEIRT GLIKGSGTAE VELKKGAALK VTLDNAFMEN
160 170 180 190 200
CDENVLWVDY KNLIKVIDVG SKIYVDDGLI SLLVKEKGKD FVMTEVENGG
210 220 230 240 250
MLGSKKGVNL PGAAVDLPAV SEKDIQDLKF GVEQNVDMVF ASFIRKAADV
260 270 280 290 300
HAVRKVLGEK GKHIKIISKI ENHEGVRRFD EIMEASDGIM VARGDLGIEI
310 320 330 340 350
PAEKVFLAQK MMIGRCNRAG KPIICATQML ESMIKKPRPT RAEGSDVANA
360 370 380 390 400
VLDGADCIML SGETAKGDYP LEAVRMQHAI AREAEAAMFH RQQFEEILRH
410 420 430 440 450
SVHHREPADA MAAGAVEASF KCLAAALIVM TESGRSAHLV SRYRPRAPII
460 470 480 490 500
AVTRNDQTAR QAHLYRGVFP VLCKQPAHDA WAEDVDLRVN LGMNVGKARG
510 520 530
FFKTGDLVIV LTGWRPGSGY TNTMRVVPVP
Length:530
Mass (Da):58,015
Last modified:January 23, 2007 - v2
Checksum:i8B0DF09FD82EB72F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00903 mRNA. Translation: AAA49021.1.
M18793
, M10619, M18788, M18789, M18790, M18791, M18792 Genomic DNA. Translation: AAA49020.1.
PIRiI50408. KICHPM.
RefSeqiNP_990800.1. NM_205469.1.
UniGeneiGga.4299.

Genome annotation databases

EnsembliENSGALT00000003100; ENSGALP00000003096; ENSGALG00000001992.
GeneIDi396456.
KEGGigga:396456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00903 mRNA. Translation: AAA49021.1 .
M18793
, M10619 , M18788 , M18789 , M18790 , M18791 , M18792 Genomic DNA. Translation: AAA49020.1 .
PIRi I50408. KICHPM.
RefSeqi NP_990800.1. NM_205469.1.
UniGenei Gga.4299.

3D structure databases

ProteinModelPortali P00548.
SMRi P00548. Positions 12-530.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 676705. 2 interactions.
IntActi P00548. 1 interaction.
STRINGi 9031.ENSGALP00000034108.

Proteomic databases

PaxDbi P00548.
PRIDEi P00548.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000003100 ; ENSGALP00000003096 ; ENSGALG00000001992 .
GeneIDi 396456.
KEGGi gga:396456.

Organism-specific databases

CTDi 5315.

Phylogenomic databases

eggNOGi COG0469.
GeneTreei ENSGT00390000008859.
HOGENOMi HOG000021559.
HOVERGENi HBG000941.
InParanoidi P00548.
KOi K00873.
PhylomeDBi P00548.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
Reactomei REACT_115767. Glycolysis.
REACT_247441. Glycolysis.

Miscellaneous databases

NextBioi 20816497.
PROi P00548.

Gene expression databases

ExpressionAtlasi P00548. baseline.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of chicken muscle pyruvate kinase mRNA."
    Lonberg N., Gilbert W.
    Proc. Natl. Acad. Sci. U.S.A. 80:3661-3665(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Intron/exon structure of the chicken pyruvate kinase gene."
    Lonberg N., Gilbert W.
    Cell 40:81-90(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYM_CHICK
AccessioniPrimary (citable) accession number: P00548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This activity is regulated by glucose levels.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3