Reviewed, UniProtKB/Swiss-Prot P00548 (KPYK_CHICK)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase muscle isozyme
    EC=2.7.1.40

Gene names

Name:

PKM2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer.
Miscellaneous	This activity is regulated by glucose levels.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 530	529	Pyruvate kinase muscle isozyme		PRO_0000112097
Sites
	Active site	269	1	By similarity
	Metal binding	271	1	Magnesium Potential
	Metal binding	292	1	Magnesium Potential
	Metal binding	293	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P00548-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8B0DF09FD82EB72F	FASTA	530	58,015
	10 20 30 40 50 60 MSKHHDAGTA FIQTQQLHAA MADTFLEHMC RLDIDSEPTI ARNTGIICTI GPASRSVDKL 70 80 90 100 110 120 KEMIKSGMNV ARLNFSHGTH EYHEGTIKNV REATESFASD PITYRPVAIA LDTKGPEIRT 130 140 150 160 170 180 GLIKGSGTAE VELKKGAALK VTLDNAFMEN CDENVLWVDY KNLIKVIDVG SKIYVDDGLI 190 200 210 220 230 240 SLLVKEKGKD FVMTEVENGG MLGSKKGVNL PGAAVDLPAV SEKDIQDLKF GVEQNVDMVF 250 260 270 280 290 300 ASFIRKAADV HAVRKVLGEK GKHIKIISKI ENHEGVRRFD EIMEASDGIM VARGDLGIEI 310 320 330 340 350 360 PAEKVFLAQK MMIGRCNRAG KPIICATQML ESMIKKPRPT RAEGSDVANA VLDGADCIML 370 380 390 400 410 420 SGETAKGDYP LEAVRMQHAI AREAEAAMFH RQQFEEILRH SVHHREPADA MAAGAVEASF 430 440 450 460 470 480 KCLAAALIVM TESGRSAHLV SRYRPRAPII AVTRNDQTAR QAHLYRGVFP VLCKQPAHDA 490 500 510 520 530 WAEDVDLRVN LGMNVGKARG FFKTGDLVIV LTGWRPGSGY TNTMRVVPVP

References

[1]	"Primary structure of chicken muscle pyruvate kinase mRNA." Lonberg N., Gilbert W. Proc. Natl. Acad. Sci. U.S.A. 80:3661-3665(1983) [PubMed: 6574503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Intron/exon structure of the chicken pyruvate kinase gene." Lonberg N., Gilbert W. Cell 40:81-90(1985) [PubMed: 2981634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J00903 mRNA. Translation: AAA49021.1. M18793 , M10619, M18788, M18789, M18790, M18791, M18792 Genomic DNA. Translation: AAA49020.1.
IPI	IPI00574064.
PIR	KICHPM. I50408.
RefSeq	NP_990800.1.
UniGene	Gga.4299
3D structure databases
HSSP	HSSP built from PDB template 1PKN based on UniProtKB P11974.
SMR	P00548. Positions 10-530.
ModBase	Search...
Proteomic databases
PRIDE	P00548.
Genome annotation databases
Ensembl	ENSGALG00000001992. Gallus gallus. [Contig view]
GeneID	396456.
KEGG	gga:396456.
Phylogenomic databases
HOVERGEN	P00548.
Enzyme and pathway databases
BRENDA	2.7.1.40. 4.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_CHICK

Accession

Primary (citable) accession number: P00548

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)