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Protein

Homoserine kinase

Gene

thrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is also able to phosphorylate the hydroxy group on gamma-carbon of L-homoserine analogs when the functional group at the alpha-position is a carboxyl, an ester, or even a hydroxymethyl group. Neither L-threonine nor L-serine are substrates of the enzyme.1 Publication

Catalytic activityi

ATP + L-homoserine = ADP + O-phospho-L-homoserine.1 Publication

Kineticsi

The catalytic efficiency is 500-fold higher with L-homoserine than with D-homoserine as substrate.

  1. KM=0.14 mM for L-homoserine1 Publication
  2. KM=31.8 mM for D-homoserine1 Publication
  3. KM=0.13 mM for ATP1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi91 – 10111ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. homoserine kinase activity Source: EcoCyc

GO - Biological processi

  1. threonine biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HOMOSERKIN-MONOMER.
ECOL316407:JW0002-MONOMER.
MetaCyc:HOMOSERKIN-MONOMER.
UniPathwayiUPA00050; UER00064.

Names & Taxonomyi

Protein namesi
Recommended name:
Homoserine kinase (EC:2.7.1.39)
Short name:
HK
Short name:
HSK
Gene namesi
Name:thrB
Ordered Locus Names:b0003, JW0002
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10999. thrB.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1391H → L: 35-fold decrease in kinase activity. 1 Publication
Mutagenesisi203 – 2031H → L: 2-fold decrease in kinase activity but nearly no change in substrates affinity. 1 Publication
Mutagenesisi206 – 2061H → Q: 3500-fold decrease in kinase activity. 1 Publication
Mutagenesisi235 – 2351R → H: 250-fold decrease in kinase activity but no change in substrates affinity. 1 Publication
Mutagenesisi235 – 2351R → L: 26200-fold decrease in catalytic efficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Homoserine kinasePRO_0000156567Add
BLAST

Proteomic databases

PaxDbiP00547.
PRIDEiP00547.

2D gel databases

SWISS-2DPAGEP00547.

Expressioni

Gene expression databases

GenevestigatoriP00547.

Interactioni

Protein-protein interaction databases

IntActiP00547. 2 interactions.
STRINGi511145.b0003.

Structurei

3D structure databases

ProteinModelPortaliP00547.
SMRiP00547. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0083.
HOGENOMiHOG000247198.
InParanoidiP00547.
KOiK00872.
OMAiGFDDWLW.
OrthoDBiEOG6DG2WK.
PhylomeDBiP00547.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG
60 70 80 90 100
RFADKLPSEP RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA
110 120 130 140 150
CSVVAALMAM NEHCGKPLND TRLLALMGEL EGRISGSIHY DNVAPCFLGG
160 170 180 190 200
MQLMIEENDI ISQQVPGFDE WLWVLAYPGI KVSTAEARAI LPAQYRRQDC
210 220 230 240 250
IAHGRHLAGF IHACYSRQPE LAAKLMKDVI AEPYRERLLP GFRQARQAVA
260 270 280 290 300
EIGAVASGIS GSGPTLFALC DKPETAQRVA DWLGKNYLQN QEGFVHICRL
310
DTAGARVLEN
Length:310
Mass (Da):33,624
Last modified:April 1, 1993 - v2
Checksum:i0F225F9F1B634BE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13601 Genomic DNA. Translation: AAA20618.1.
U14003 Genomic DNA. Translation: AAA97302.1.
U00096 Genomic DNA. Translation: AAC73114.1.
AP009048 Genomic DNA. Translation: BAB96580.2.
PIRiS56630. KIECM.
RefSeqiNP_414544.1. NC_000913.3.
YP_488309.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73114; AAC73114; b0003.
BAB96580; BAB96580; BAB96580.
GeneIDi12932940.
947498.
KEGGiecj:Y75_p0003.
eco:b0003.
PATRICi32115101. VBIEscCol129921_0002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13601 Genomic DNA. Translation: AAA20618.1.
U14003 Genomic DNA. Translation: AAA97302.1.
U00096 Genomic DNA. Translation: AAC73114.1.
AP009048 Genomic DNA. Translation: BAB96580.2.
PIRiS56630. KIECM.
RefSeqiNP_414544.1. NC_000913.3.
YP_488309.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP00547.
SMRiP00547. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00547. 2 interactions.
STRINGi511145.b0003.

Chemistry

BindingDBiP00547.

2D gel databases

SWISS-2DPAGEP00547.

Proteomic databases

PaxDbiP00547.
PRIDEiP00547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73114; AAC73114; b0003.
BAB96580; BAB96580; BAB96580.
GeneIDi12932940.
947498.
KEGGiecj:Y75_p0003.
eco:b0003.
PATRICi32115101. VBIEscCol129921_0002.

Organism-specific databases

EchoBASEiEB0992.
EcoGeneiEG10999. thrB.

Phylogenomic databases

eggNOGiCOG0083.
HOGENOMiHOG000247198.
InParanoidiP00547.
KOiK00872.
OMAiGFDDWLW.
OrthoDBiEOG6DG2WK.
PhylomeDBiP00547.

Enzyme and pathway databases

UniPathwayiUPA00050; UER00064.
BioCyciEcoCyc:HOMOSERKIN-MONOMER.
ECOL316407:JW0002-MONOMER.
MetaCyc:HOMOSERKIN-MONOMER.

Miscellaneous databases

PROiP00547.

Gene expression databases

GenevestigatoriP00547.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00384. Homoser_kinase.
InterProiIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR000870. Homoserine_kinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000676. Homoser_kin. 1 hit.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00191. thrB. 1 hit.
PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions."
    Cossart P., Katinka M., Yaniv M.
    Nucleic Acids Res. 9:339-347(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Update on the nucleotide sequence of the thrB gene of E. coli."
    Deborde D.C., Strange J.C., Wright B.E.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION TO 166-190.
  3. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  4. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  7. "Substrate specificity and identification of functional groups of homoserine kinase from Escherichia coli."
    Huo X., Viola R.E.
    Biochemistry 35:16180-16185(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A HOMOSERINE KINASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS, MUTAGENESIS OF HIS-139; HIS-203; HIS-206 AND ARG-235.

Entry informationi

Entry nameiKHSE_ECOLI
AccessioniPrimary (citable) accession number: P00547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.