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P00547

- KHSE_ECOLI

UniProt

P00547 - KHSE_ECOLI

Protein

Homoserine kinase

Gene

thrB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is also able to phosphorylate the hydroxy group on gamma-carbon of L-homoserine analogs when the functional group at the alpha-position is a carboxyl, an ester, or even a hydroxymethyl group. Neither L-threonine nor L-serine are substrates of the enzyme.1 Publication

    Catalytic activityi

    ATP + L-homoserine = ADP + O-phospho-L-homoserine.1 Publication

    Kineticsi

    The catalytic efficiency is 500-fold higher with L-homoserine than with D-homoserine as substrate.

    1. KM=0.14 mM for L-homoserine1 Publication
    2. KM=31.8 mM for D-homoserine1 Publication
    3. KM=0.13 mM for ATP1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi91 – 10111ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. homoserine kinase activity Source: EcoCyc

    GO - Biological processi

    1. threonine biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Threonine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:HOMOSERKIN-MONOMER.
    ECOL316407:JW0002-MONOMER.
    MetaCyc:HOMOSERKIN-MONOMER.
    UniPathwayiUPA00050; UER00064.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homoserine kinase (EC:2.7.1.39)
    Short name:
    HK
    Short name:
    HSK
    Gene namesi
    Name:thrB
    Ordered Locus Names:b0003, JW0002
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10999. thrB.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi139 – 1391H → L: 35-fold decrease in kinase activity. 1 Publication
    Mutagenesisi203 – 2031H → L: 2-fold decrease in kinase activity but nearly no change in substrates affinity. 1 Publication
    Mutagenesisi206 – 2061H → Q: 3500-fold decrease in kinase activity. 1 Publication
    Mutagenesisi235 – 2351R → H: 250-fold decrease in kinase activity but no change in substrates affinity. 1 Publication
    Mutagenesisi235 – 2351R → L: 26200-fold decrease in catalytic efficiency. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Homoserine kinasePRO_0000156567Add
    BLAST

    Proteomic databases

    PaxDbiP00547.
    PRIDEiP00547.

    2D gel databases

    SWISS-2DPAGEP00547.

    Expressioni

    Gene expression databases

    GenevestigatoriP00547.

    Interactioni

    Protein-protein interaction databases

    IntActiP00547. 2 interactions.
    STRINGi511145.b0003.

    Structurei

    3D structure databases

    ProteinModelPortaliP00547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0083.
    HOGENOMiHOG000247198.
    KOiK00872.
    OMAiHIANEGT.
    OrthoDBiEOG6DG2WK.
    PhylomeDBiP00547.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPiMF_00384. Homoser_kinase.
    InterProiIPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR000870. Homoserine_kinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PfamiPF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000676. Homoser_kin. 1 hit.
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsiTIGR00191. thrB. 1 hit.
    PROSITEiPS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00547-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG    50
    RFADKLPSEP RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA 100
    CSVVAALMAM NEHCGKPLND TRLLALMGEL EGRISGSIHY DNVAPCFLGG 150
    MQLMIEENDI ISQQVPGFDE WLWVLAYPGI KVSTAEARAI LPAQYRRQDC 200
    IAHGRHLAGF IHACYSRQPE LAAKLMKDVI AEPYRERLLP GFRQARQAVA 250
    EIGAVASGIS GSGPTLFALC DKPETAQRVA DWLGKNYLQN QEGFVHICRL 300
    DTAGARVLEN 310
    Length:310
    Mass (Da):33,624
    Last modified:April 1, 1993 - v2
    Checksum:i0F225F9F1B634BE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13601 Genomic DNA. Translation: AAA20618.1.
    U14003 Genomic DNA. Translation: AAA97302.1.
    U00096 Genomic DNA. Translation: AAC73114.1.
    AP009048 Genomic DNA. Translation: BAB96580.2.
    PIRiS56630. KIECM.
    RefSeqiNP_414544.1. NC_000913.3.
    YP_488309.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73114; AAC73114; b0003.
    BAB96580; BAB96580; BAB96580.
    GeneIDi12932940.
    947498.
    KEGGiecj:Y75_p0003.
    eco:b0003.
    PATRICi32115101. VBIEscCol129921_0002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L13601 Genomic DNA. Translation: AAA20618.1 .
    U14003 Genomic DNA. Translation: AAA97302.1 .
    U00096 Genomic DNA. Translation: AAC73114.1 .
    AP009048 Genomic DNA. Translation: BAB96580.2 .
    PIRi S56630. KIECM.
    RefSeqi NP_414544.1. NC_000913.3.
    YP_488309.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P00547.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P00547. 2 interactions.
    STRINGi 511145.b0003.

    Chemistry

    BindingDBi P00547.

    2D gel databases

    SWISS-2DPAGE P00547.

    Proteomic databases

    PaxDbi P00547.
    PRIDEi P00547.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73114 ; AAC73114 ; b0003 .
    BAB96580 ; BAB96580 ; BAB96580 .
    GeneIDi 12932940.
    947498.
    KEGGi ecj:Y75_p0003.
    eco:b0003.
    PATRICi 32115101. VBIEscCol129921_0002.

    Organism-specific databases

    EchoBASEi EB0992.
    EcoGenei EG10999. thrB.

    Phylogenomic databases

    eggNOGi COG0083.
    HOGENOMi HOG000247198.
    KOi K00872.
    OMAi HIANEGT.
    OrthoDBi EOG6DG2WK.
    PhylomeDBi P00547.

    Enzyme and pathway databases

    UniPathwayi UPA00050 ; UER00064 .
    BioCyci EcoCyc:HOMOSERKIN-MONOMER.
    ECOL316407:JW0002-MONOMER.
    MetaCyc:HOMOSERKIN-MONOMER.

    Miscellaneous databases

    PROi P00547.

    Gene expression databases

    Genevestigatori P00547.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.70.890. 1 hit.
    HAMAPi MF_00384. Homoser_kinase.
    InterProi IPR013750. GHMP_kinase_C_dom.
    IPR006204. GHMP_kinase_N_dom.
    IPR006203. GHMP_knse_ATP-bd_CS.
    IPR000870. Homoserine_kinase.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    Pfami PF08544. GHMP_kinases_C. 1 hit.
    PF00288. GHMP_kinases_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000676. Homoser_kin. 1 hit.
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55060. SSF55060. 1 hit.
    TIGRFAMsi TIGR00191. thrB. 1 hit.
    PROSITEi PS00627. GHMP_KINASES_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions."
      Cossart P., Katinka M., Yaniv M.
      Nucleic Acids Res. 9:339-347(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Update on the nucleotide sequence of the thrB gene of E. coli."
      Deborde D.C., Strange J.C., Wright B.E.
      Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION TO 166-190.
    3. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
      Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
      Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12.
    4. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Substrate specificity and identification of functional groups of homoserine kinase from Escherichia coli."
      Huo X., Viola R.E.
      Biochemistry 35:16180-16185(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A HOMOSERINE KINASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS, MUTAGENESIS OF HIS-139; HIS-203; HIS-206 AND ARG-235.

    Entry informationi

    Entry nameiKHSE_ECOLI
    AccessioniPrimary (citable) accession number: P00547
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3