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Protein

Cyclin-dependent kinase 1

Gene

CDC28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is essential for the completion of the start, the controlling event, in the cell cycle. More than 200 substrates have been identified.

Miscellaneous

Present with 6670 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation at Thr-18 or Tyr-19 inactivates the enzyme, while phosphorylation at Thr-169 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • histone binding Source: SGD
  • protein serine/threonine kinase activity Source: SGD
  • RNA polymerase II core binding Source: SGD

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: SGD
  • cell differentiation Source: GO_Central
  • cell division Source: UniProtKB-KW
  • cellular protein catabolic process Source: SGD
  • DNA double-strand break processing Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • histone phosphorylation Source: GO_Central
  • meiotic DNA double-strand break processing Source: SGD
  • mitotic cell cycle phase transition Source: GO_Central
  • mitotic sister chromatid biorientation Source: SGD
  • mitotic spindle assembly Source: SGD
  • negative regulation of double-strand break repair via nonhomologous end joining Source: SGD
  • negative regulation of meiotic cell cycle Source: SGD
  • negative regulation of mitotic cell cycle Source: SGD
  • negative regulation of sister chromatid cohesion Source: SGD
  • negative regulation of transcription, DNA-templated Source: SGD
  • negative regulation of transcription by RNA polymerase II Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • peptidyl-threonine phosphorylation Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of glycogen catabolic process Source: SGD
  • positive regulation of meiotic cell cycle Source: SGD
  • positive regulation of mitotic cell cycle Source: SGD
  • positive regulation of mitotic spindle pole body separation Source: SGD
  • positive regulation of nuclear cell cycle DNA replication Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of trehalose catabolic process Source: SGD
  • positive regulation of triglyceride catabolic process Source: SGD
  • post-Golgi vesicle-mediated transport Source: SGD
  • protein localization to nuclear periphery Source: SGD
  • protein localization to nucleus Source: SGD
  • protein localization to spindle microtubule Source: SGD
  • regulation of budding cell apical bud growth Source: SGD
  • regulation of double-strand break repair via homologous recombination Source: SGD
  • regulation of filamentous growth Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of protein localization to chromatin Source: SGD
  • regulation of spindle assembly Source: SGD
  • regulation of telomere maintenance via telomerase Source: SGD
  • synaptonemal complex assembly Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29110-MONOMER
BRENDAi2.7.11.22 984
ReactomeiR-SCE-110056 MAPK3 (ERK1) activation
R-SCE-176408 Regulation of APC/C activators between G1/S and early anaphase
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-2299718 Condensation of Prophase Chromosomes
R-SCE-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-SCE-4419969 Depolymerisation of the Nuclear Lamina
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-SCE-68949 Orc1 removal from chromatin
R-SCE-68962 Activation of the pre-replicative complex
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69202 Cyclin E associated events during G1/S transition
R-SCE-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-SCE-69478 G2/M DNA replication checkpoint
R-SCE-69656 Cyclin A:Cdk2-associated events at S phase entry
R-SCE-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22)
Short name:
CDK1
Alternative name(s):
Cell division control protein 28
Cell division protein kinase 1
Gene namesi
Name:CDC28
Synonyms:CDK1, HSL5, SRM5
Ordered Locus Names:YBR160W
ORF Names:YBR1211
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR160W
SGDiS000000364 CDC28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5213

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000857222 – 298Cyclin-dependent kinase 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei19PhosphotyrosineCombined sources1
Modified residuei169PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00546
PaxDbiP00546
PRIDEiP00546

PTM databases

iPTMnetiP00546

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.

Binary interactionsi

Show more details

GO - Molecular functioni

  • histone binding Source: SGD
  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi32859, 1278 interactors
DIPiDIP-1039N
IntActiP00546, 87 interactors
MINTiP00546
STRINGi4932.YBR160W

Chemistry databases

BindingDBiP00546

Structurei

3D structure databases

ProteinModelPortaliP00546
SMRiP00546
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 295Protein kinasePROSITE-ProRule annotationAdd BLAST288

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00910000144030
InParanoidiP00546
KOiK04563
OMAiEMMLVYD
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG
60 70 80 90 100
VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME
110 120 130 140 150
GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL
160 170 180 190 200
KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC
210 220 230 240 250
IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP
260 270 280 290
QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES
Length:298
Mass (Da):34,061
Last modified:July 21, 1986 - v1
Checksum:i57A7A2B97A90DC6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00257 Genomic DNA Translation: CAA25065.1
Z36029 Genomic DNA Translation: CAA85119.1
X80224 Genomic DNA Translation: CAA56509.1
BK006936 Genomic DNA Translation: DAA07275.1
PIRiA00657 TVBY8
RefSeqiNP_009718.3, NM_001178508.3

Genome annotation databases

EnsemblFungiiYBR160W; YBR160W; YBR160W
GeneIDi852457
KEGGisce:YBR160W

Similar proteinsi

Entry informationi

Entry nameiCDK1_YEAST
AccessioniPrimary (citable) accession number: P00546
Secondary accession number(s): D6VQF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 202 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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