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P00546

- CDK1_YEAST

UniProt

P00546 - CDK1_YEAST

Protein

Cyclin-dependent kinase 1

Gene

CDC28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 163 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    This protein is essential for the completion of the start, the controlling event, in the cell cycle. More than 200 substrates have been identified.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Phosphorylation at Thr-18 or Tyr-19 inactivates the enzyme, while phosphorylation at Thr-169 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401ATPPROSITE-ProRule annotation
    Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 229ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: SGD
    3. histone binding Source: SGD
    4. protein binding Source: IntAct
    5. protein serine/threonine kinase activity Source: SGD
    6. RNA polymerase II core binding Source: SGD

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: SGD
    2. chromatin remodeling Source: SGD
    3. meiotic DNA double-strand break processing Source: SGD
    4. mitotic nuclear division Source: UniProtKB-KW
    5. negative regulation of double-strand break repair via nonhomologous end joining Source: SGD
    6. negative regulation of meiotic cell cycle Source: SGD
    7. negative regulation of mitotic cell cycle Source: SGD
    8. negative regulation of sister chromatid cohesion Source: SGD
    9. negative regulation of transcription, DNA-templated Source: SGD
    10. peptidyl-serine phosphorylation Source: SGD
    11. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
    12. positive regulation of meiotic cell cycle Source: SGD
    13. positive regulation of mitotic cell cycle Source: SGD
    14. positive regulation of nuclear cell cycle DNA replication Source: SGD
    15. positive regulation of spindle pole body separation Source: SGD
    16. positive regulation of transcription, DNA-templated Source: SGD
    17. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    18. positive regulation of triglyceride catabolic process Source: SGD
    19. regulation of budding cell apical bud growth Source: SGD
    20. regulation of double-strand break repair via homologous recombination Source: SGD
    21. regulation of filamentous growth Source: SGD
    22. regulation of protein localization Source: SGD
    23. synaptonemal complex assembly Source: SGD
    24. vesicle-mediated transport Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29110-MONOMER.
    BRENDAi2.7.11.22. 984.
    ReactomeiREACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188488. G2/M DNA replication checkpoint.
    REACT_188908. Condensation of Prometaphase Chromosomes.
    REACT_188916. MASTL Facilitates Mitotic Progression.
    REACT_189247. G1/S-Specific Transcription.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224510. Depolymerisation of the Nuclear Lamina.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 28
    Cell division protein kinase 1
    Gene namesi
    Name:CDC28
    Synonyms:CDK1, HSL5, SRM5
    Ordered Locus Names:YBR160W
    ORF Names:YBR1211
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR160w.
    SGDiS000000364. CDC28.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cyclin-dependent protein kinase holoenzyme complex Source: SGD
    3. cytoplasm Source: SGD
    4. endoplasmic reticulum Source: SGD
    5. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 298297Cyclin-dependent kinase 1PRO_0000085722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei19 – 191Phosphotyrosine2 Publications
    Modified residuei169 – 1691Phosphothreonine; alternate2 Publications
    Modified residuei169 – 1691Phosphothreonine; by CAK; alternateBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP00546.
    PaxDbiP00546.
    PeptideAtlasiP00546.

    Expressioni

    Gene expression databases

    GenevestigatoriP00546.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P030703EBI-4253,EBI-617698From a different organism.
    CAK1P435683EBI-4253,EBI-3953
    CDC6P091192EBI-4253,EBI-4447
    CKS1P204867EBI-4253,EBI-4746
    CLB5P302833EBI-4253,EBI-4538
    CLN1P204376EBI-4253,EBI-4479
    CLN3P133655EBI-4253,EBI-4490

    Protein-protein interaction databases

    BioGridi32859. 567 interactions.
    DIPiDIP-1039N.
    IntActiP00546. 71 interactions.
    MINTiMINT-569037.
    STRINGi4932.YBR160W.

    Structurei

    3D structure databases

    ProteinModelPortaliP00546.
    SMRiP00546. Positions 17-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 295288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108415.
    KOiK04563.
    OMAiLVHRFFR.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00546-1 [UniParc]FASTAAdd to Basket

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    MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG    50
    VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME 100
    GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL 150
    KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC 200
    IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP 250
    QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES 298
    Length:298
    Mass (Da):34,061
    Last modified:July 21, 1986 - v1
    Checksum:i57A7A2B97A90DC6C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00257 Genomic DNA. Translation: CAA25065.1.
    Z36029 Genomic DNA. Translation: CAA85119.1.
    X80224 Genomic DNA. Translation: CAA56509.1.
    BK006936 Genomic DNA. Translation: DAA07275.1.
    PIRiA00657. TVBY8.
    RefSeqiNP_009718.3. NM_001178508.3.

    Genome annotation databases

    EnsemblFungiiYBR160W; YBR160W; YBR160W.
    GeneIDi852457.
    KEGGisce:YBR160W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00257 Genomic DNA. Translation: CAA25065.1 .
    Z36029 Genomic DNA. Translation: CAA85119.1 .
    X80224 Genomic DNA. Translation: CAA56509.1 .
    BK006936 Genomic DNA. Translation: DAA07275.1 .
    PIRi A00657. TVBY8.
    RefSeqi NP_009718.3. NM_001178508.3.

    3D structure databases

    ProteinModelPortali P00546.
    SMRi P00546. Positions 17-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32859. 567 interactions.
    DIPi DIP-1039N.
    IntActi P00546. 71 interactions.
    MINTi MINT-569037.
    STRINGi 4932.YBR160W.

    Chemistry

    BindingDBi P00546.
    ChEMBLi CHEMBL5213.

    Proteomic databases

    MaxQBi P00546.
    PaxDbi P00546.
    PeptideAtlasi P00546.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR160W ; YBR160W ; YBR160W .
    GeneIDi 852457.
    KEGGi sce:YBR160W.

    Organism-specific databases

    CYGDi YBR160w.
    SGDi S000000364. CDC28.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108415.
    KOi K04563.
    OMAi LVHRFFR.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29110-MONOMER.
    BRENDAi 2.7.11.22. 984.
    Reactomei REACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_188488. G2/M DNA replication checkpoint.
    REACT_188908. Condensation of Prometaphase Chromosomes.
    REACT_188916. MASTL Facilitates Mitotic Progression.
    REACT_189247. G1/S-Specific Transcription.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224510. Depolymerisation of the Nuclear Lamina.

    Miscellaneous databases

    NextBioi 971387.

    Gene expression databases

    Genevestigatori P00546.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes."
      Lorincz A.T., Reed S.I.
      Nature 307:183-185(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of a 5.6 kb fragment of chromosome II from Saccharomyces cerevisiae reveals two new open reading frames next to CDC28."
      Baur S., Becker J., Li Z., Niegemann E., Wehner E., Wolter R., Brendel M.
      Yeast 11:455-458(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: PHOSPHORYLATION SUBSTRATES.
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-19 AND THR-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDK1_YEAST
    AccessioniPrimary (citable) accession number: P00546
    Secondary accession number(s): D6VQF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 163 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6670 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3