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P00546

- CDK1_YEAST

UniProt

P00546 - CDK1_YEAST

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Protein

Cyclin-dependent kinase 1

Gene
CDC28, CDK1, HSL5, SRM5, YBR160W, YBR1211
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is essential for the completion of the start, the controlling event, in the cell cycle. More than 200 substrates have been identified.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation at Thr-18 or Tyr-19 inactivates the enzyme, while phosphorylation at Thr-169 activates it By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATP By similarity
Active sitei136 – 1361Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 229ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: SGD
  3. histone binding Source: SGD
  4. protein binding Source: IntAct
  5. protein serine/threonine kinase activity Source: SGD
  6. RNA polymerase II core binding Source: SGD

GO - Biological processi

  1. 7-methylguanosine mRNA capping Source: SGD
  2. chromatin remodeling Source: SGD
  3. meiotic DNA double-strand break processing Source: SGD
  4. mitotic nuclear division Source: UniProtKB-KW
  5. negative regulation of double-strand break repair via nonhomologous end joining Source: SGD
  6. negative regulation of meiotic cell cycle Source: SGD
  7. negative regulation of mitotic cell cycle Source: SGD
  8. negative regulation of sister chromatid cohesion Source: SGD
  9. negative regulation of transcription, DNA-templated Source: SGD
  10. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  11. positive regulation of meiotic cell cycle Source: SGD
  12. positive regulation of mitotic cell cycle Source: SGD
  13. positive regulation of nuclear cell cycle DNA replication Source: SGD
  14. positive regulation of spindle pole body separation Source: SGD
  15. positive regulation of transcription, DNA-templated Source: SGD
  16. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  17. positive regulation of triglyceride catabolic process Source: SGD
  18. regulation of budding cell apical bud growth Source: SGD
  19. regulation of double-strand break repair via homologous recombination Source: SGD
  20. regulation of filamentous growth Source: SGD
  21. regulation of protein localization Source: SGD
  22. synaptonemal complex assembly Source: SGD
  23. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29110-MONOMER.
BRENDAi2.7.11.22. 984.
ReactomeiREACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188488. G2/M DNA replication checkpoint.
REACT_188908. Condensation of Prometaphase Chromosomes.
REACT_188916. MASTL Facilitates Mitotic Progression.
REACT_189247. G1/S-Specific Transcription.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_224510. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22)
Short name:
CDK1
Alternative name(s):
Cell division control protein 28
Cell division protein kinase 1
Gene namesi
Name:CDC28
Synonyms:CDK1, HSL5, SRM5
Ordered Locus Names:YBR160W
ORF Names:YBR1211
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR160w.
SGDiS000000364. CDC28.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cyclin-dependent protein kinase holoenzyme complex Source: SGD
  3. cytoplasm Source: SGD
  4. endoplasmic reticulum Source: SGD
  5. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 298297Cyclin-dependent kinase 1PRO_0000085722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei19 – 191Phosphotyrosine1 Publication
Modified residuei169 – 1691Phosphothreonine; alternate1 Publication
Modified residuei169 – 1691Phosphothreonine; by CAK; alternate By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00546.
PaxDbiP00546.
PeptideAtlasiP00546.

Expressioni

Gene expression databases

GenevestigatoriP00546.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.

Binary interactionsi

WithEntry#Exp.IntActNotes
P030703EBI-4253,EBI-617698From a different organism.
CAK1P435683EBI-4253,EBI-3953
CDC6P091192EBI-4253,EBI-4447
CKS1P204867EBI-4253,EBI-4746
CLB5P302833EBI-4253,EBI-4538
CLN1P204376EBI-4253,EBI-4479
CLN3P133655EBI-4253,EBI-4490

Protein-protein interaction databases

BioGridi32859. 567 interactions.
DIPiDIP-1039N.
IntActiP00546. 71 interactions.
MINTiMINT-569037.
STRINGi4932.YBR160W.

Structurei

3D structure databases

ProteinModelPortaliP00546.
SMRiP00546. Positions 17-297.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 295288Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00720000108415.
KOiK04563.
OMAiLVHRFFR.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00546-1 [UniParc]FASTAAdd to Basket

« Hide

MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG    50
VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME 100
GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL 150
KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC 200
IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP 250
QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES 298
Length:298
Mass (Da):34,061
Last modified:July 21, 1986 - v1
Checksum:i57A7A2B97A90DC6C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00257 Genomic DNA. Translation: CAA25065.1.
Z36029 Genomic DNA. Translation: CAA85119.1.
X80224 Genomic DNA. Translation: CAA56509.1.
BK006936 Genomic DNA. Translation: DAA07275.1.
PIRiA00657. TVBY8.
RefSeqiNP_009718.3. NM_001178508.3.

Genome annotation databases

EnsemblFungiiYBR160W; YBR160W; YBR160W.
GeneIDi852457.
KEGGisce:YBR160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00257 Genomic DNA. Translation: CAA25065.1 .
Z36029 Genomic DNA. Translation: CAA85119.1 .
X80224 Genomic DNA. Translation: CAA56509.1 .
BK006936 Genomic DNA. Translation: DAA07275.1 .
PIRi A00657. TVBY8.
RefSeqi NP_009718.3. NM_001178508.3.

3D structure databases

ProteinModelPortali P00546.
SMRi P00546. Positions 17-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32859. 567 interactions.
DIPi DIP-1039N.
IntActi P00546. 71 interactions.
MINTi MINT-569037.
STRINGi 4932.YBR160W.

Chemistry

BindingDBi P00546.
ChEMBLi CHEMBL5213.

Proteomic databases

MaxQBi P00546.
PaxDbi P00546.
PeptideAtlasi P00546.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR160W ; YBR160W ; YBR160W .
GeneIDi 852457.
KEGGi sce:YBR160W.

Organism-specific databases

CYGDi YBR160w.
SGDi S000000364. CDC28.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00720000108415.
KOi K04563.
OMAi LVHRFFR.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BioCyci YEAST:G3O-29110-MONOMER.
BRENDAi 2.7.11.22. 984.
Reactomei REACT_188466. Regulation of APC/C activators between G1/S and early anaphase.
REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_188488. G2/M DNA replication checkpoint.
REACT_188908. Condensation of Prometaphase Chromosomes.
REACT_188916. MASTL Facilitates Mitotic Progression.
REACT_189247. G1/S-Specific Transcription.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_224510. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

NextBioi 971387.

Gene expression databases

Genevestigatori P00546.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes."
    Lorincz A.T., Reed S.I.
    Nature 307:183-185(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 5.6 kb fragment of chromosome II from Saccharomyces cerevisiae reveals two new open reading frames next to CDC28."
    Baur S., Becker J., Li Z., Niegemann E., Wehner E., Wolter R., Brendel M.
    Yeast 11:455-458(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION SUBSTRATES.
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-19 AND THR-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDK1_YEAST
AccessioniPrimary (citable) accession number: P00546
Secondary accession number(s): D6VQF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6670 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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