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Protein

Cyclin-dependent kinase 1

Gene

CDC28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is essential for the completion of the start, the controlling event, in the cell cycle. More than 200 substrates have been identified.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation at Thr-18 or Tyr-19 inactivates the enzyme, while phosphorylation at Thr-169 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 22ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • histone binding Source: SGD
  • protein serine/threonine kinase activity Source: SGD
  • RNA polymerase II core binding Source: SGD

GO - Biological processi

  • 7-methylguanosine mRNA capping Source: SGD
  • cell division Source: UniProtKB-KW
  • meiotic DNA double-strand break processing Source: SGD
  • mitotic sister chromatid biorientation Source: SGD
  • negative regulation of double-strand break repair via nonhomologous end joining Source: SGD
  • negative regulation of meiotic cell cycle Source: SGD
  • negative regulation of mitotic cell cycle Source: SGD
  • negative regulation of sister chromatid cohesion Source: SGD
  • negative regulation of transcription, DNA-templated Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • peptidyl-threonine phosphorylation Source: SGD
  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of glycogen catabolic process Source: SGD
  • positive regulation of meiotic cell cycle Source: SGD
  • positive regulation of mitotic cell cycle Source: SGD
  • positive regulation of nuclear cell cycle DNA replication Source: SGD
  • positive regulation of spindle pole body separation Source: SGD
  • positive regulation of transcription, DNA-templated Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of trehalose catabolic process Source: SGD
  • positive regulation of triglyceride catabolic process Source: SGD
  • protein localization to nuclear periphery Source: SGD
  • protein localization to nucleus Source: SGD
  • protein phosphorylation involved in cellular protein catabolic process Source: SGD
  • protein phosphorylation involved in DNA double-strand break processing Source: SGD
  • protein phosphorylation involved in double-strand break repair via nonhomologous end joining Source: SGD
  • protein phosphorylation involved in mitotic spindle assembly Source: SGD
  • protein phosphorylation involved in protein localization to spindle microtubule Source: SGD
  • regulation of budding cell apical bud growth Source: SGD
  • regulation of double-strand break repair via homologous recombination Source: SGD
  • regulation of filamentous growth Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of spindle assembly Source: SGD
  • regulation of telomere maintenance via telomerase Source: SGD
  • synaptonemal complex assembly Source: SGD
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29110-MONOMER.
BRENDAi2.7.11.22. 984.
ReactomeiR-SCE-110056. MAPK3 (ERK1) activation.
R-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22)
Short name:
CDK1
Alternative name(s):
Cell division control protein 28
Cell division protein kinase 1
Gene namesi
Name:CDC28
Synonyms:CDK1, HSL5, SRM5
Ordered Locus Names:YBR160W
ORF Names:YBR1211
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR160W.
SGDiS000000364. CDC28.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • endoplasmic reticulum Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000857222 – 298Cyclin-dependent kinase 1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei19PhosphotyrosineCombined sources1
Modified residuei169PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP00546.
PRIDEiP00546.

PTM databases

iPTMnetiP00546.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.

Binary interactionsi

WithEntry#Exp.IntActNotes
P030703EBI-4253,EBI-617698From a different organism.
CAK1P435683EBI-4253,EBI-3953
CDC6P091192EBI-4253,EBI-4447
CKS1P204868EBI-4253,EBI-4746
CLB5P302833EBI-4253,EBI-4538
CLN1P204376EBI-4253,EBI-4479
CLN2P204389EBI-4253,EBI-4483
CLN3P133656EBI-4253,EBI-4490

GO - Molecular functioni

  • histone binding Source: SGD
  • RNA polymerase II core binding Source: SGD

Protein-protein interaction databases

BioGridi32859. 782 interactors.
DIPiDIP-1039N.
IntActiP00546. 71 interactors.
MINTiMINT-569037.

Chemistry databases

BindingDBiP00546.

Structurei

3D structure databases

ProteinModelPortaliP00546.
SMRiP00546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 295Protein kinasePROSITE-ProRule annotationAdd BLAST288

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00830000128256.
InParanoidiP00546.
KOiK04563.
OMAiPKWQRDY.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG
60 70 80 90 100
VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME
110 120 130 140 150
GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL
160 170 180 190 200
KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC
210 220 230 240 250
IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP
260 270 280 290
QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES
Length:298
Mass (Da):34,061
Last modified:July 21, 1986 - v1
Checksum:i57A7A2B97A90DC6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00257 Genomic DNA. Translation: CAA25065.1.
Z36029 Genomic DNA. Translation: CAA85119.1.
X80224 Genomic DNA. Translation: CAA56509.1.
BK006936 Genomic DNA. Translation: DAA07275.1.
PIRiA00657. TVBY8.
RefSeqiNP_009718.3. NM_001178508.3.

Genome annotation databases

EnsemblFungiiYBR160W; YBR160W; YBR160W.
GeneIDi852457.
KEGGisce:YBR160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00257 Genomic DNA. Translation: CAA25065.1.
Z36029 Genomic DNA. Translation: CAA85119.1.
X80224 Genomic DNA. Translation: CAA56509.1.
BK006936 Genomic DNA. Translation: DAA07275.1.
PIRiA00657. TVBY8.
RefSeqiNP_009718.3. NM_001178508.3.

3D structure databases

ProteinModelPortaliP00546.
SMRiP00546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32859. 782 interactors.
DIPiDIP-1039N.
IntActiP00546. 71 interactors.
MINTiMINT-569037.

Chemistry databases

BindingDBiP00546.
ChEMBLiCHEMBL5213.

PTM databases

iPTMnetiP00546.

Proteomic databases

MaxQBiP00546.
PRIDEiP00546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR160W; YBR160W; YBR160W.
GeneIDi852457.
KEGGisce:YBR160W.

Organism-specific databases

EuPathDBiFungiDB:YBR160W.
SGDiS000000364. CDC28.

Phylogenomic databases

GeneTreeiENSGT00830000128256.
InParanoidiP00546.
KOiK04563.
OMAiPKWQRDY.
OrthoDBiEOG092C2FL8.

Enzyme and pathway databases

BioCyciYEAST:G3O-29110-MONOMER.
BRENDAi2.7.11.22. 984.
ReactomeiR-SCE-110056. MAPK3 (ERK1) activation.
R-SCE-113507. E2F-enabled inhibition of pre-replication complex formation.
R-SCE-113510. E2F mediated regulation of DNA replication.
R-SCE-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-SCE-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-SCE-3214858. RMTs methylate histone arginines.
R-SCE-4419969. Depolymerisation of the Nuclear Lamina.
R-SCE-5687128. MAPK6/MAPK4 signaling.
R-SCE-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-SCE-69202. Cyclin E associated events during G1/S transition.
R-SCE-69205. G1/S-Specific Transcription.
R-SCE-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.
R-SCE-69478. G2/M DNA replication checkpoint.
R-SCE-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-SCE-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP00546.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK1_YEAST
AccessioniPrimary (citable) accession number: P00546
Secondary accession number(s): D6VQF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 187 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.