P00545 (KFMS_FSVMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transforming protein fms
EC=2.7.10.1

Gene names

Name:

V-FMS

Organism

Feline sarcoma virus (strain McDonough)

Taxonomic identifier

11778 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication competent viruses › Feline leukemia virus › Feline sarcoma virus

Virus host

Felidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length	978 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Truncated version of the receptor for colony-stimulating factor 1 (CSF-1).
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Miscellaneous	This protein is synthesized as a Gag-Fms polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Disease	Oncogene
Domain	Immunoglobulin domain Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological_process	transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 978	978	Tyrosine-protein kinase transforming protein fms		PRO_0000155209
Regions
	Topological domain	1 – 543	543	Extracellular Potential
	Transmembrane	544 – 568	25	Helical; Potential
	Topological domain	569 – 978	410	Cytoplasmic Potential
	Domain	55 – 134	80	Ig-like C2-type 1
	Domain	141 – 231	91	Ig-like C2-type 2
	Domain	236 – 331	96	Ig-like C2-type 3
	Domain	333 – 431	99	Ig-like C2-type 4
	Domain	434 – 533	100	Ig-like C2-type 5
	Domain	613 – 942	330	Protein kinase
	Nucleotide binding	619 – 627	9	ATP By similarity
Sites
	Active site	810	1	Proton acceptor By similarity
	Binding site	647	1	ATP By similarity
Amino acid modifications
	Modified residue	841	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	973	1	Phosphothreonine Ref.2
	Glycosylation	79	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	107	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	128	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	187	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	309	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	320	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	336	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	369	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	444	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	511	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	524	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	76 ↔ 118		Potential
	Disulfide bond	161 ↔ 211		Potential
	Disulfide bond	258 ↔ 312		Potential
	Disulfide bond	451 ↔ 516		Potential
Experimental info
	Sequence conflict	714	1	L → P in AAA43045. Ref.1
	Sequence conflict	971 – 978	8	QRTPPVAR → RGPPL in AAA43045. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P00545 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 4C7CAC4835185EBF	FASTA	978	108,492
	10 20 30 40 50 60 RMPSGPGHYG ASAETPGPRP PLCPASSCCL PTEAMGPRAL LVLLMATAWH AQGVPVIQPS 70 80 90 100 110 120 GPELVVEPGT TVTLRCVGNG SVEWDGPISP HWNLDLDPPS SILTTNNATF QNTGTYHCTE 130 140 150 160 170 180 PGNPRGGNAT IHLYVKDPAR PWKVLAQEVT VLEGQDALLP CLLTDPALEA GVSLVRVRGR 190 200 210 220 230 240 PVLRQTNYSF SPWHGFTIHK AKFIENHVYQ CSARVDGRTV TSMGIWLKVQ KDISGPATLT 250 260 270 280 290 300 LEPAELVRIQ GEAAQIVCSA SNIDVNFDVS LRHGDTKLTI SQQSDFHDNR YQKVLTLNLD 310 320 330 340 350 360 HVSFQDAGNY SCTATNAWGN HSASMVFRVV ESAYSNLTSE QSLLQEVTVG EKVDLQVKVE 370 380 390 400 410 420 AYPGLESFNW TYLGPFSDYQ DKLDFVTIKD TYRYTSTLSL PRLKRSESGR YSFLARNAGG 430 440 450 460 470 480 QNALTFELTL RYPPEVRVTM TLINGSDTLL CEASGYPQPS VTWVQCRSHT DRCDESAGLV 490 500 510 520 530 540 LEDSHSEVLS QVPFYEVIVH SLLAIGTLEH NRTYECRAFN SVGNSSQTFW PISIGAHTPL 550 560 570 580 590 600 PDELLFTPVL LTCMSIMALL LLLLLLLLYK YKQKPKYQVR WKIIESYEGN SYTFIDPTQL 610 620 630 640 650 660 PYNEKWEFPR NNLQFGKTLG TGAFGKVVEA TAFGLGKEDA VLKVAVKMLK STAHADEKEA 670 680 690 700 710 720 LMSELKIMSH LGQHENIVNL LGACTHGGPV LVITEYCCYG DLLNFLRRQA EAMLGPSLSV 730 740 750 760 770 780 GQDPEAGAGY KNIHLEKKYV RRDSGFSSQG VDTYVEMRPV STSSSNDSFS EEDLGKEDGR 790 800 810 820 830 840 PLELRDLLHF SSQVAQGMAF LASKNCIHRD VAARNVLLTS GRVAKIGDFG LARDIMNDSN 850 860 870 880 890 900 YIVKGNARLP VKWMAPESIF DCVYTVQSDV WSYGILLWEI FSLGLNPYPG ILVNSKFYKL 910 920 930 940 950 960 VKDGYQMAQP AFAPKNIYSI MQACWALEPT RRPTFQQICS LLQKQAQEDR RVPNYTNLPS 970 SSSSRLLRPW QRTPPVAR

References

[1]	"Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases." Hampe A., Gobet M., Sherr C.J., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 81:85-89(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Reassessment of the v-fms sequence: threonine phosphorylation of the COOH-terminal domain." Smola U., Hennig D., Hadwiger-Fangmeier A., Schuetz B., Pfaff E., Niemann H., Tamura T. J. Virol. 65:6181-6187(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, PHOSPHORYLATION AT THR-973.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01643 Genomic RNA. Translation: AAA43045.1. S59588 Genomic DNA. Translation: AAB20028.1.
PIR	TVMVMD. A00654.
3D structure databases
ProteinModelPortal	P00545.
SMR	P00545. Positions 578-951.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 2234.
Family and domain databases
Gene3D	2.60.40.10. 5 hits.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR013151. Immunoglobulin. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. IPR016243. Tyr_kinase_CSF1/PDGF_rcpt. IPR001824. Tyr_kinase_rcpt_3_CS. [Graphical view]
Pfam	PF00047. ig. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PIRSF	PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMART	SM00409. IG. 3 hits. SM00408. IGc2. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50835. IG_LIKE. 3 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00240. RECEPTOR_TYR_KIN_III. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KFMS_FSVMD

Accession

Primary (citable) accession number: P00545
Secondary accession number(s): Q86597

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program