P00541 (FPS_AVISP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transforming protein Fps
EC=2.7.10.2

Gene names

Name:

V-FPS

Organism

Avian sarcoma virus (strain PRCII)

Taxonomic identifier

11880 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Alpharetrovirus › Fujinami sarcoma virus

Virus host

Galliformes [TaxID: 8976]

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Miscellaneous	This protein is synthesized as a Gag-Fps polyprotein. Present in the avian viruses PRCII and Fujinami sarcoma virus (FSV). PRCII is less oncogenic than FSV and has a slightly different v-Fps protein.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Disease	Oncogene
Domain	SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	viral reproduction Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Tyrosine-protein kinase transforming protein Fps		PRO_0000088094
Regions
	Domain	50 – 127	78	FCH
	Domain	171 – 260	90	SH2
	Domain	272 – 525	254	Protein kinase
	Nucleotide binding	278 – 286	9	ATP By similarity
Sites
	Active site	394	1	Proton acceptor By similarity
	Binding site	301	1	ATP By similarity
Amino acid modifications
	Modified residue	424	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00541 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 2F765877C2522D8A	FASTA	533	60,506
	10 20 30 40 50 60 ASGQLHRPQP QEHTSTSAAA GTWRHTQASE SRHRLPHCSA APSHQDHSAM GFGPELWCPK 70 80 90 100 110 120 GHSELLRLQD SELRLLELMK KWMSERAKSD REYAGMLHHM FSQLGSEEPP PALPLQEDRQ 130 140 150 160 170 180 SVCSTDQERS GVTALETIKN HISGIFSPRF SLPPPVPLIP EVQKPLCQQA WYHGAIPRSE 190 200 210 220 230 240 VQELLKCSGD FLVRESQGKQ EYVLSVLWDG QPRHFIIQAA DNLYRLEGDG FPTIPLLIDH 250 260 270 280 290 300 LLQSQQPITR KSGIVLTRAV LKDKWVLNHE DVLLGERIGR GNFGEVFSGR LRADNTPVAV 310 320 330 340 350 360 KSCRETLPPE LKAKFLQEAR ILKQYNHPNI VRLIGVCTQK QPIYIVMELV QGGDFLSFLR 370 380 390 400 410 420 SKGPHLKMKE LIKMMENAAA GMEYLESKHC IHRDLAARNC LVTEKNTLKI SDFGMSRQEE 430 440 450 460 470 480 DGVYASTGGM KQIPVKWTAP EALNYGRYSS ESDVWSFGIL LWEAFSLGAV PYANLSNQQT 490 500 510 520 530 REAIEQGVRL EPPEQCPEDV YRLMQRCWEY DPRRRPSFGA VHQDLIAIRK RHR

References

[1]	"Nucleotide sequence of v-fps in the PRCII strain of avian sarcoma virus." Huang C.-C., Hammond C., Bishop J.M. J. Virol. 50:125-131(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01690 Genomic RNA. Translation: AAA42415.1.
PIR	TVFVFP. A00650.
3D structure databases
ProteinModelPortal	P00541.
SMR	P00541. Positions 160-532.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 600.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR001060. FCH_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008919. Retrov_capsid_N. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
SMART	SM00055. FCH. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF47943. Retrov_capsid_N. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FPS_AVISP

Accession

Primary (citable) accession number: P00541

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program