ID   EGFR_HUMAN     STANDARD;      PRT;  1210 AA.
AC   P00533; P06268; Q14225;
DT   21-JUL-1986 (REL. 01, CREATED)
DT   01-NOV-1997 (REL. 35, LAST SEQUENCE UPDATE)
DT   15-DEC-1998 (REL. 37, LAST ANNOTATION UPDATE)
DE   EPIDERMAL GROWTH FACTOR RECEPTOR PRECURSOR (EC 2.7.1.112).
GN   EGFR OR ERBB1.
OS   HOMO SAPIENS (HUMAN).
OC   EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; MAMMALIA; EUTHERIA;
OC   PRIMATES; CATARRHINI; HOMINIDAE; HOMO.
RN   [1]
RP   SEQUENCE FROM N.A.
RX   MEDLINE; 84219729.
RA   ULLRICH A., COUSSENS L., HAYFLICK J.S., DULL T.J., GRAY A., TAM A.W.,
RA   LEE J., YARDEN Y., LIBERMANN T.A., SCHLESSINGER J., DOWNWARD J.,
RA   MAYES E.L.V., WHITTLE N., WATERFIELD M.D., SEEBURG P.H.;
RT   "Human epidermal growth factor receptor cDNA sequence and aberrant
RT   expression of the amplified gene in A431 epidermoid carcinoma
RT   cells.";
RL   NATURE 309:418-425(1984).
RN   [2]
RP   SEQUENCE OF 713-924 FROM N.A.
RX   MEDLINE; 84196372.
RA   LIN C.R., CHEN W.S., KRUIGER W., STOLARSKY L.S., WEBER W.,
RA   EVANS R.M., VERMA I.M., GILL G.N., ROSENFELD M.G.;
RT   "Expression cloning of human EGF receptor complementary DNA: gene
RT   amplification and three related messenger RNA products in A431
RT   cells.";
RL   SCIENCE 224:843-848(1984).
RN   [3]
RP   SEQUENCE OF 150-962 FROM N.A.
RX   MEDLINE; 84245835.
RA   XU Y., ISHII S., CLARK A.J.L., SULLIVAN M., WILSON R.K., MA D.P.,
RA   ROE B.A., MERLINO G.T., PASTAN I.;
RT   "Human epidermal growth factor receptor cDNA is homologous to a
RT   variety of RNAs overproduced in A431 carcinoma cells.";
RL   NATURE 309:806-810(1984).
RN   [4]
RP   SEQUENCE OF 1028-1210 FROM N.A.
RX   MEDLINE; 85046483.
RA   SIMMEN F.A., GOPE M.L., SCHULZ T.Z., WRIGHT D.A., CARPENTER G.,
RA   O'MALLEY B.W.;
RT   "Isolation of an evolutionarily conserved epidermal growth factor
RT   receptor cDNA from human A431 carcinoma cells.";
RL   BIOCHEM. BIOPHYS. RES. COMMUN. 124:125-132(1984).
RN   [5]
RP   SEQUENCE OF 1-29 FROM N.A.
RX   MEDLINE; 88217333.
RA   HALEY J., WHITTLE N., BENNETT P., KINCHINGTON D., ULLRICH A.,
RA   WATERFIELD M.;
RT   "The human EGF receptor gene: structure of the 110 kb locus and
RT   identification of sequences regulating its transcription.";
RL   ONCOGENE RES. 1:375-396(1987).
RN   [6]
RP   SEQUENCE OF 1-29 FROM N.A.
RX   MEDLINE; 91107677.
RA   HALEY J.D., WATERFIELD M.D.;
RT   "Contributory effects of de novo transcription and premature
RT   transcript termination in the regulation of human epidermal growth
RT   factor receptor proto-oncogene RNA synthesis.";
RL   J. BIOL. CHEM. 266:1746-1753(1991).
RN   [7]
RP   SEQUENCE OF 1-29 FROM N.A.
RX   MEDLINE; 85270438.
RA   ISHII S., XU Y.H., STRATTON R.H., ROE B.A., MERLINO G.T., PASTAN I.;
RT   "Characterization and sequence of the promoter region of the human
RT   epidermal growth factor receptor gene.";
RL   PROC. NATL. ACAD. SCI. U.S.A. 82:4920-4924(1985).
RN   [8]
RP   SEQUENCE OF 540.
RA   KOHDA D.;
RL   SUBMITTED (SEP-1997) TO THE SWISS-PROT DATA BANK.
RN   [9]
RP   RECEPTOR ACTIVITY.
RX   MEDLINE; 84191554.
RA   MROCZKOWSKI B., MOSIG G., COHEN S.;
RT   "ATP-stimulated interaction between epidermal growth factor receptor
RT   and supercoiled DNA.";
RL   NATURE 309:270-273(1984).
RN   [10]
RP   PHOSPHORYLATION.
RX   MEDLINE; 89278137.
RA   MARGOLIS B.L., LAX I., KRIS R., DOMBALAGIAN M., HONEGGER A.M.,
RA   HOWK R., GIVOL D., ULLRICH A., SCHLESSINGER J.;
RT   "All autophosphorylation sites of epidermal growth factor (EGF)
RT   receptor and HER2/neu are located in their carboxyl-terminal tails.
RT   Identification of a novel site in EGF receptor.";
RL   J. BIOL. CHEM. 264:10667-10671(1989).
RN   [11]
RP   REVIEW.
RX   MEDLINE; 87297456.
RA   CARPENTER G.;
RT   "Receptors for epidermal growth factor and other polypeptide
RT   mitogens.";
RL   ANNU. REV. BIOCHEM. 56:881-914(1987).
CC   -!- FUNCTION: THE EGF RECEPTOR MEDIATES THE BIOLOGICAL SIGNAL OF EGF,
CC       AND ALSO OF TGF-ALPHA, AMPHIREGULIN, HEPARIN-BINDING EGF, GP30 AND
CC       VACCINIA VIRUS GROWTH FACTOR.
CC   -!- CATALYTIC ACTIVITY: ATP + A PROTEIN TYROSINE = ADP +
CC       PROTEIN TYROSINE PHOSPHATE.
CC   -!- SUBCELLULAR LOCATION: TYPE I MEMBRANE PROTEIN.
CC   -!- BINDING OF EGF TO THE RECEPTOR LEADS TO INTERNALIZATION OF THE
CC       EGF-RECEPTOR COMPLEX, INDUCTION OF THE TYROSINE KINASE ACTIVITY,
CC       STIMULATION OF CELL DNA SYNTHESIS, AND CELL PROLIFERATION.
CC   -!- SIMILARITY: BELONGS TO THE EGF RECEPTOR FAMILY.
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DR   EMBL; X00588; G757924; -.
DR   EMBL; X06370; G31119; -.
DR   EMBL; X00663; E4014; -.
DR   EMBL; M38425; G553271; -.
DR   EMBL; M11234; G553272; -.
DR   PIR; A00641; GQHUE.
DR   PIR; A00642; GQHUE2.
DR   PIR; A23062; A23062.
DR   SWISS-2DPAGE; P00533; HUMAN.
DR   MIM; 131550; -.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PFAM; PF00037; fer4; 1.
DR   PFAM; PF00069; pkinase; 1.
DR   PFAM; PF00757; Furin-like; 1.
DR   PFAM; PF01030; Recep_L_domain; 2.
DR   HSSP; P11362; 1FGI.
KW   TRANSMEMBRANE; GLYCOPROTEIN; DUPLICATION; RECEPTOR; SIGNAL;
KW   TRANSFERASE; TYROSINE-PROTEIN KINASE; ATP-BINDING; PHOSPHORYLATION.
FT   SIGNAL        1     24
FT   CHAIN        25   1210       EGF RECEPTOR.
FT   DOMAIN       25    645       EXTRACELLULAR (POTENTIAL).
FT   TRANSMEM    646    668       POTENTIAL.
FT   DOMAIN      669   1210       CYTOPLASMIC (POTENTIAL).
FT   REPEAT       75    300       APPROXIMATE.
FT   REPEAT      390    600       APPROXIMATE.
FT   DOMAIN     1025   1071       SER-RICH.
FT   DOMAIN      712    979       PROTEIN KINASE.
FT   NP_BIND     718    726       ATP (BY SIMILARITY).
FT   BINDING     745    745       ATP (BY SIMILARITY).
FT   ACT_SITE    837    837       BY SIMILARITY.
FT   MOD_RES     678    678       PHOSPHORYLATION (BY PKC).
FT   MOD_RES    1092   1092       PHOSPHORYLATION (AUTO-).
FT   MOD_RES    1110   1110       PHOSPHORYLATION (AUTO-).
FT   MOD_RES    1172   1172       PHOSPHORYLATION (AUTO-).
FT   MOD_RES    1197   1197       PHOSPHORYLATION (AUTO-, MAJOR SITE).
FT   CARBOHYD    128    128       POTENTIAL.
FT   CARBOHYD    175    175       POTENTIAL.
FT   CARBOHYD    196    196       POTENTIAL.
FT   CARBOHYD    352    352       POTENTIAL.
FT   CARBOHYD    361    361       POTENTIAL.
FT   CARBOHYD    413    413       POTENTIAL.
FT   CARBOHYD    444    444       POTENTIAL.
FT   CARBOHYD    528    528       POTENTIAL.
FT   CARBOHYD    568    568       POTENTIAL.
FT   CARBOHYD    603    603       POTENTIAL.
FT   CARBOHYD    623    623       POTENTIAL.
FT   CONFLICT    540    540       N -> K (IN REF. 1).
SQ   SEQUENCE   1210 AA;  134277 MW;  19DDD2E4 CRC32;
     MRPSGTAGAA LLALLAALCP ASRALEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV
     VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA
     VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF
     QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC
     TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV
     VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK
     NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF
     ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL
     FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCN
     LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM
     GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIATGM VGALLLLLVV
     ALGIGLFMRR RHIVRKRTLR RLLQERELVE PLTPSGEAPN QALLRILKET EFKKIKVLGS
     GAFGTVYKGL WIPEGEKVKI PVAIKELREA TSPKANKEIL DEAYVMASVD NPHVCRLLGI
     CLTSTVQLIT QLMPFGCLLD YVREHKDNIG SQYLLNWCVQ IAKGMNYLED RRLVHRDLAA
     RNVLVKTPQH VKITDFGLAK LLGAEEKEYH AEGGKVPIKW MALESILHRI YTHQSDVWSY
     GVTVWELMTF GSKPYDGIPA SEISSILEKG ERLPQPPICT IDVYMIMVKC WMIDADSRPK
     FRELIIEFSK MARDPQRYLV IQGDERMHLP SPTDSNFYRA LMDEEDMDDV VDADEYLIPQ
     QGFFSSPSTS RTPLLSSLSA TSNNSTVACI DRNGLQSCPI KEDSFLQRYS SDPTGALTED
     SIDDTFLPVP EYINQSVPKR PAGSVQNPVY HNQPLNPAPS RDPHYQDPHS TAVGNPEYLN
     TVQPTCVNST FDSPAHWAQK GSHQISLDNP DYQQDFFPKE AKPNGIFKGS TAENAEYLRV
     APQSSEFIGA
//