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P00532 (RAF_MSV36) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase-transforming protein raf

EC=2.7.11.1
Gene names
Name:V-RAF
OrganismMurine sarcoma virus 3611
Taxonomic identifier11812 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Miscellaneous

This protein is synthesized as a Gag-Raf polyprotein.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAA46579.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Serine/threonine-protein kinase-transforming protein raf
PRO_0000086601

Regions

Domain24 – 284261Protein kinase
Nucleotide binding30 – 389ATP By similarity

Sites

Active site1431Proton acceptor By similarity
Binding site501ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P00532 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 52A5423A66E362F3

FASTA32336,884
        10         20         30         40         50         60 
EKNKIRPRGQ RDSSYYWKME ASEVMLSTRI GSGSFGTVYK GKWHGDVAVK ILKVVDPTPE 

        70         80         90        100        110        120 
QLQAFRNEVA VLRKTRHVNI LLFMGYMTKD NLAIVTQWCE GSSLYKHLHV QETKFQMFQL 

       130        140        150        160        170        180 
IDIARQTAQG MDYLHAKNII HRDMKSNNIF LHEGLTVKIG DFGLATVKSR WSGSQQVEQP 

       190        200        210        220        230        240 
TGSVLWMAPE VIRMQDDNPF SFQSDVYSYG IVLYELMAGE LPYAHINNRD QIIFMVGRGY 

       250        260        270        280        290        300 
ASPDLSRLYK NCPKAIKRLV ADCVKKVKEE RPLFPQILSS IELLQHSLPK INRSAPEPSL 

       310        320 
HRAAHTEDIN ACTLTTSPRL PVF 

« Hide

References

[1]"A common onc gene sequence transduced by avian carcinoma virus MH2 and by murine sarcoma virus 3611."
Kan N.C., Flordellis C.S., Mark G.E., Duesberg P.H., Papas T.S.
Science 223:813-816(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Primary structure of v-raf: relatedness to the src family of oncogenes."
Mark G.E., Rapp U.R.
Science 224:285-289(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K01691 Genomic RNA. Translation: AAA46579.1. Different initiation.
PIRTVMVF6. A00638.

3D structure databases

ProteinModelPortalP00532.
SMRP00532. Positions 17-290.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.10.2. 3471.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRAF_MSV36
AccessionPrimary (citable) accession number: P00532
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 16, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families