P00532 (RAF_MSV36) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase-transforming protein raf
EC=2.7.11.1

Gene names

Name:

V-RAF

Organism

Murine sarcoma virus 3611

Taxonomic identifier

11812 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication defective viruses › Moloney murine sarcoma virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	This protein is synthesized as a Gag-Raf polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAA46579.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Disease	Oncogene
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Serine/threonine-protein kinase-transforming protein raf		PRO_0000086601
Regions
	Domain	24 – 284	261	Protein kinase
	Nucleotide binding	30 – 38	9	ATP By similarity
Sites
	Active site	143	1	Proton acceptor By similarity
	Binding site	50	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00532 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 52A5423A66E362F3	FASTA	323	36,884
	10 20 30 40 50 60 EKNKIRPRGQ RDSSYYWKME ASEVMLSTRI GSGSFGTVYK GKWHGDVAVK ILKVVDPTPE 70 80 90 100 110 120 QLQAFRNEVA VLRKTRHVNI LLFMGYMTKD NLAIVTQWCE GSSLYKHLHV QETKFQMFQL 130 140 150 160 170 180 IDIARQTAQG MDYLHAKNII HRDMKSNNIF LHEGLTVKIG DFGLATVKSR WSGSQQVEQP 190 200 210 220 230 240 TGSVLWMAPE VIRMQDDNPF SFQSDVYSYG IVLYELMAGE LPYAHINNRD QIIFMVGRGY 250 260 270 280 290 300 ASPDLSRLYK NCPKAIKRLV ADCVKKVKEE RPLFPQILSS IELLQHSLPK INRSAPEPSL 310 320 HRAAHTEDIN ACTLTTSPRL PVF

References

[1]	"A common onc gene sequence transduced by avian carcinoma virus MH2 and by murine sarcoma virus 3611." Kan N.C., Flordellis C.S., Mark G.E., Duesberg P.H., Papas T.S. Science 223:813-816(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Primary structure of v-raf: relatedness to the src family of oncogenes." Mark G.E., Rapp U.R. Science 224:285-289(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K01691 Genomic RNA. Translation: AAA46579.1. Different initiation.
PIR	TVMVF6. A00638.
3D structure databases
ProteinModelPortal	P00532.
SMR	P00532. Positions 17-290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 3471.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAF_MSV36

Accession

Primary (citable) accession number: P00532

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program