P00530 (FPS_FUJSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transforming protein Fps
EC=2.7.10.2

Gene names

Name:

V-FPS

Organism

Fujinami sarcoma virus

Taxonomic identifier

11885 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Alpharetrovirus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Miscellaneous	This protein is synthesized as a Gag-Fps polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
Sequence caution	The sequence AAA42402.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Disease	Oncogene
Domain	SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: InterPro viral reproduction Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Tyrosine-protein kinase transforming protein Fps		PRO_0000088096
Regions
	Domain	50 – 122	73	FCH
	Domain	511 – 600	90	SH2
	Domain	612 – 865	254	Protein kinase
	Nucleotide binding	618 – 626	9	ATP By similarity
Sites
	Active site	734	1	Proton acceptor By similarity
	Binding site	641	1	ATP By similarity
Amino acid modifications
	Modified residue	764	1	Phosphotyrosine; by autocatalysis By similarity
Natural variations
	Natural variant	63	1	T → S in clone TS.
	Natural variant	251	1	H → R in clone TS.
	Natural variant	300	1	K → E in clone TS.
	Natural variant	343	1	N → S in clone TS.
	Natural variant	438	1	A → T in clone TS.
	Natural variant	447	1	E → D in clone TS.
	Natural variant	463	1	R → C in clone TS.
	Natural variant	716	1	E → D in clone TS.

Sequences

	Sequence		Length	Mass (Da)
	P00530 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 1D774D48B18466B6	FASTA	873	99,536
	10 20 30 40 50 60 ASGQLHRPQP QEHTSTSAAA GTWRLTQASE SRHRLPHCSA APSHQDHSAM GFGPELWCPK 70 80 90 100 110 120 GHTELLRLQD SELRLLELMK KWMSQRAKSD REYAGMLHHM FSQLEKQEGL GHLRATDHSS 130 140 150 160 170 180 QIGESWWVLA SQTETLSQTL RRHAEELAAG PLAKLSILIR DKQQLRKVFS EQWQQLSQEY 190 200 210 220 230 240 AWTTQQEVEK LKAQYRSLVR DSTQAKRKYQ EASKDKEREK AKEKYVRSLS KLYALHNQYV 250 260 270 280 290 300 LAVQAAALHH HHHYQRALPT LHESLYSLQQ EMVLVLKEIL GEYCSITSLV QEDVLAIHQK 310 320 330 340 350 360 VAHAVEMIDP ATEYSSFVQC HRYDSEVPPA VTFDESLLEE AENLEPGELQ LNELTIESVQ 370 380 390 400 410 420 HSLTSIEEEL LASRKAVSSK EQRVWELQVE LRGEELALSP GERVHLLGKR QGLREAQQQL 430 440 450 460 470 480 QGLVCAQAKL QAQRDMLANK LAELGSEEPP PALPLQEDRQ SARSTDQERS GVTALKTIKN 490 500 510 520 530 540 HISGIFSPRF SLPPPVPLIP EVQKPLCQQA WYHGAIPRSE VQELLKYSGD FLVRESQGKQ 550 560 570 580 590 600 EYVLSVLWDG QPRHFIIQAA DNLYRLEDDG LPTIPLLIDH LLQSQRPITR KSGIVLTRAV 610 620 630 640 650 660 LKDKWVLNHE DVLLGERIGR GNFGEVFSGR LRADNTPVAV KSCRETLPPE LKAKFLQEAR 670 680 690 700 710 720 ILKQCNHPNI VRLIGVCTQK QPIYIVMELV QGGDFLSFLR SKGPRLKMKK LIKMMENAAA 730 740 750 760 770 780 GMEYLESKHC IHRDLAARNC LVTEKNTLKI SDFGMSRQEE DGVYASTGGM KQIPVKWTAP 790 800 810 820 830 840 EALNYGWYSS ESDVWSFGIL LWEAFSLGAV PYANLSNQQT REAIEQGVRL EPPEQCPEDV 850 860 870 YRLMQRCWEY DPHRRPSFGA VHQDLIAIRK RHR

References

[1]	"Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses." Shibuya M., Hanafusa H. Cell 30:787-795(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Single amino acid substitution, from Glu1025 to Asp, of the fps oncogenic protein causes temperature sensitivity in transformation and kinase activity." Chen L.H., Hatada E., Wheatley W., Lee W.H. Virology 155:106-119(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] (CLONE TS).

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02194 Genomic RNA. Translation: AAA42402.1. Different initiation. M14930 Genomic RNA. Translation: AAA42403.1.
PIR	TVFVF. A00636. TVFVFS. A26898.
RefSeq	NP_955606.1. NC_001403.1.
3D structure databases
ProteinModelPortal	P00530.
SMR	P00530. Positions 500-872.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491921.
Enzyme and pathway databases
BRENDA	2.7.10.2. 2335.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR001060. FCH_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008919. Retrov_capsid_N. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. IPR016250. Tyr_kinase_non-rcpt_Fes_subgr. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PIRSF	PIRSF000632. TyrPK_fps. 1 hit.
PRINTS	PR00109. TYRKINASE.
SMART	SM00055. FCH. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF47943. Retrov_capsid_N. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P00530.
ChEMBL	CHEMBL5708.

Entry information

Entry name

FPS_FUJSV

Accession

Primary (citable) accession number: P00530

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program