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Protein

Tyrosine-protein kinase Src64B

Gene

Src64B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the development of neural tissue and smooth muscle.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei312ATPPROSITE-ProRule annotation1
Active sitei404Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 298ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • protein tyrosine kinase activity Source: FlyBase
  • receptor binding Source: FlyBase

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • adherens junction organization Source: FlyBase
  • axon guidance Source: FlyBase
  • cellularization Source: FlyBase
  • dorsal closure Source: FlyBase
  • epithelial cell-cell adhesion Source: FlyBase
  • female germline ring canal formation Source: FlyBase
  • female germline ring canal formation, actin assembly Source: FlyBase
  • female germline ring canal stabilization Source: FlyBase
  • filtration diaphragm assembly Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • germarium-derived female germ-line cyst encapsulation Source: FlyBase
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • karyosome formation Source: FlyBase
  • learning or memory Source: FlyBase
  • long-term memory Source: FlyBase
  • mushroom body development Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • olfactory learning Source: FlyBase
  • oogenesis Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian fusome organization Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • parallel actin filament bundle assembly Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • positive regulation of protein kinase activity Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of actin polymerization or depolymerization Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of synaptic plasticity Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-1433557. Signaling by SCF-KIT.
R-DME-180292. GAB1 signalosome.
R-DME-2454202. Fc epsilon receptor (FCERI) signaling.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-372708. p130Cas linkage to MAPK signaling for integrins.
R-DME-373753. Nephrin interactions.
R-DME-375165. NCAM signaling for neurite out-growth.
R-DME-3928662. EPHB-mediated forward signaling.
R-DME-3928663. EPHA-mediated growth cone collapse.
R-DME-3928664. Ephrin signaling.
R-DME-3928665. EPH-ephrin mediated repulsion of cells.
R-DME-418592. ADP signalling through P2Y purinoceptor 1.
R-DME-418885. DCC mediated attractive signaling.
R-DME-430116. GP1b-IX-V activation signalling.
R-DME-432142. Platelet sensitization by LDL.
R-DME-437239. Recycling pathway of L1.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-5673000. RAF activation.
R-DME-5673001. RAF/MAP kinase cascade.
R-DME-6798695. Neutrophil degranulation.
R-DME-8874081. MET activates PTK2 signaling.
R-DME-912631. Regulation of signaling by CBL.
SignaLinkiP00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Src64B (EC:2.7.10.2)
Short name:
Dsrc64
Gene namesi
Name:Src64B
Synonyms:Src1
ORF Names:CG7524
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0262733. Src64B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: FlyBase
  • extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
  • germline ring canal Source: FlyBase
  • plasma membrane Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881401 – 552Tyrosine-protein kinase Src64BAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei434Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00528.
PRIDEiP00528.

PTM databases

iPTMnetiP00528.

Expressioni

Tissue specificityi

After the first 8 hours of development, accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal disks, and in differentiating smooth muscle.1 Publication

Developmental stagei

Abundant in embryos and pupae, rare in larvae and adults.1 Publication

Gene expression databases

BgeeiFBgn0262733.
ExpressionAtlasiP00528. baseline.
GenevisibleiP00528. DM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
sloQ037203EBI-87092,EBI-426805

GO - Molecular functioni

  • receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi71867. 47 interactors.
DIPiDIP-17438N.
IntActiP00528. 7 interactors.
MINTiMINT-296972.
STRINGi7227.FBpp0293527.

Structurei

3D structure databases

ProteinModelPortaliP00528.
SMRiP00528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 156SH3PROSITE-ProRule annotationAdd BLAST62
Domaini162 – 259SH2PROSITE-ProRule annotationAdd BLAST98
Domaini284 – 537Protein kinasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
InParanoidiP00528.
KOiK05704.
OrthoDBiEOG091G0D46.
PhylomeDBiP00528.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNKCCSKRQ DQELALAYPT GGYKKSDYTF GQTHINSSGG GNMGGVLGQK
60 70 80 90 100
HNNGGSLDSR YTPDPNHRGP LKIGGKGGVD IIRPRTTPTG VPGVVLKRVV
110 120 130 140 150
VALYDYKSRD ESDLSFMKGD RMEVIDDTES DWWRVVNLTT RQEGLIPLNF
160 170 180 190 200
VAEERSVNSE DWFFENVLRK EADKLLLAEE NPRGTFLVRP SEHNPNGYSL
210 220 230 240 250
SVKDWEDGRG YHVKHYRIKP LDNGGYYIAT NQTFPSLQAL VMAYSKNALG
260 270 280 290 300
LCHILSRPCP KPQPQMWDLG PELRDKYEIP RSEIQLLRKL GRGNFGEVFY
310 320 330 340 350
GKWRNSIDVA VKTLREGTMS TAAFLQEAAI MKKFRHNRLV ALYAVCSQEE
360 370 380 390 400
PIYIVQEYMS KGSLLDFLRE GDGRYLHFED LIYIATQVAS GMEYLESKQL
410 420 430 440 450
IHRDLAARNV LIGENNVAKI CDFGLARVIA DDEYCPKQGS RFPVKWTAPE
460 470 480 490 500
AIIYGKFSIK SDVWSYGILL MELFTYGQVP YPGMHSREVI ENIERGFRMP
510 520 530 540 550
KPTNHYFPDN IYQLLLQCWD AVPEKRPTFE FLNHYFESFS VTSEVPYREV

QD
Length:552
Mass (Da):63,003
Last modified:June 1, 2001 - v3
Checksum:i4A63CF4F16562864
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102A → S in AAA28913 (PubMed:2996778).Curated1
Sequence conflicti261 – 272KPQPQ…DLGPE → ASLPQTAAPDVGFGPQ in AAA28489 (PubMed:6317185).CuratedAdd BLAST12
Sequence conflicti286 – 287LL → VV in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti290L → V in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti293G → R in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti316E → A in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti366D → N in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti373G → D in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti384 – 385IA → MH in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti389 – 390AS → TT in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti393E → K in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti400L → V in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti406 – 407AA → TT in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti435C → R in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti460K → E in CAA05754 (PubMed:9731193).Curated1
Sequence conflicti471M → T in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti484M → L in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti507F → L in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti536F → L in AAA28489 (PubMed:6317185).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11917 mRNA. Translation: AAA28913.1.
AE014296 Genomic DNA. Translation: AAF47922.1.
AE014296 Genomic DNA. Translation: AAX52734.1.
AE014296 Genomic DNA. Translation: AAX52735.1.
AE014296 Genomic DNA. Translation: AAX52736.1.
AE014296 Genomic DNA. Translation: AAX52737.1.
AY051781 mRNA. Translation: AAK93205.1.
K01043 Genomic DNA. Translation: AAA28489.1.
AJ002919 Genomic DNA. Translation: CAA05754.1.
RefSeqiNP_001014561.1. NM_001014561.2.
NP_001014562.1. NM_001014562.3.
NP_001014563.1. NM_001014563.3.
NP_001014564.1. NM_001014564.3.
NP_001189051.1. NM_001202122.1.
NP_001246628.1. NM_001259699.2.
NP_001246629.1. NM_001259700.2.
NP_524934.2. NM_080195.4.
UniGeneiDm.1531.

Genome annotation databases

EnsemblMetazoaiFBtr0073321; FBpp0073177; FBgn0262733.
FBtr0100504; FBpp0099944; FBgn0262733.
FBtr0100505; FBpp0099946; FBgn0262733.
FBtr0100507; FBpp0099947; FBgn0262733.
FBtr0100508; FBpp0099948; FBgn0262733.
FBtr0302594; FBpp0291750; FBgn0262733.
FBtr0304989; FBpp0293526; FBgn0262733.
FBtr0304990; FBpp0293527; FBgn0262733.
GeneIDi48973.
KEGGidme:Dmel_CG7524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11917 mRNA. Translation: AAA28913.1.
AE014296 Genomic DNA. Translation: AAF47922.1.
AE014296 Genomic DNA. Translation: AAX52734.1.
AE014296 Genomic DNA. Translation: AAX52735.1.
AE014296 Genomic DNA. Translation: AAX52736.1.
AE014296 Genomic DNA. Translation: AAX52737.1.
AY051781 mRNA. Translation: AAK93205.1.
K01043 Genomic DNA. Translation: AAA28489.1.
AJ002919 Genomic DNA. Translation: CAA05754.1.
RefSeqiNP_001014561.1. NM_001014561.2.
NP_001014562.1. NM_001014562.3.
NP_001014563.1. NM_001014563.3.
NP_001014564.1. NM_001014564.3.
NP_001189051.1. NM_001202122.1.
NP_001246628.1. NM_001259699.2.
NP_001246629.1. NM_001259700.2.
NP_524934.2. NM_080195.4.
UniGeneiDm.1531.

3D structure databases

ProteinModelPortaliP00528.
SMRiP00528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71867. 47 interactors.
DIPiDIP-17438N.
IntActiP00528. 7 interactors.
MINTiMINT-296972.
STRINGi7227.FBpp0293527.

PTM databases

iPTMnetiP00528.

Proteomic databases

PaxDbiP00528.
PRIDEiP00528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0073321; FBpp0073177; FBgn0262733.
FBtr0100504; FBpp0099944; FBgn0262733.
FBtr0100505; FBpp0099946; FBgn0262733.
FBtr0100507; FBpp0099947; FBgn0262733.
FBtr0100508; FBpp0099948; FBgn0262733.
FBtr0302594; FBpp0291750; FBgn0262733.
FBtr0304989; FBpp0293526; FBgn0262733.
FBtr0304990; FBpp0293527; FBgn0262733.
GeneIDi48973.
KEGGidme:Dmel_CG7524.

Organism-specific databases

CTDi48973.
FlyBaseiFBgn0262733. Src64B.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
InParanoidiP00528.
KOiK05704.
OrthoDBiEOG091G0D46.
PhylomeDBiP00528.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
ReactomeiR-DME-1433557. Signaling by SCF-KIT.
R-DME-180292. GAB1 signalosome.
R-DME-2454202. Fc epsilon receptor (FCERI) signaling.
R-DME-354192. Integrin alphaIIb beta3 signaling.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-372708. p130Cas linkage to MAPK signaling for integrins.
R-DME-373753. Nephrin interactions.
R-DME-375165. NCAM signaling for neurite out-growth.
R-DME-3928662. EPHB-mediated forward signaling.
R-DME-3928663. EPHA-mediated growth cone collapse.
R-DME-3928664. Ephrin signaling.
R-DME-3928665. EPH-ephrin mediated repulsion of cells.
R-DME-418592. ADP signalling through P2Y purinoceptor 1.
R-DME-418885. DCC mediated attractive signaling.
R-DME-430116. GP1b-IX-V activation signalling.
R-DME-432142. Platelet sensitization by LDL.
R-DME-437239. Recycling pathway of L1.
R-DME-4420097. VEGFA-VEGFR2 Pathway.
R-DME-456926. Thrombin signalling through proteinase activated receptors (PARs).
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-5673000. RAF activation.
R-DME-5673001. RAF/MAP kinase cascade.
R-DME-6798695. Neutrophil degranulation.
R-DME-8874081. MET activates PTK2 signaling.
R-DME-912631. Regulation of signaling by CBL.
SignaLinkiP00528.

Miscellaneous databases

ChiTaRSiSrc64B. fly.
GenomeRNAii48973.
PROiP00528.

Gene expression databases

BgeeiFBgn0262733.
ExpressionAtlasiP00528. baseline.
GenevisibleiP00528. DM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRC64_DROME
AccessioniPrimary (citable) accession number: P00528
Secondary accession number(s): A4V1H7, O18372, Q9VZA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 188 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.