Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase Src64B

Gene

Src64B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in the development of neural tissue and smooth muscle.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei312ATPPROSITE-ProRule annotation1
Active sitei404Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 298ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • protein tyrosine kinase activity Source: FlyBase
  • signaling receptor binding Source: FlyBase

GO - Biological processi

  • actin cytoskeleton organization Source: FlyBase
  • adherens junction organization Source: FlyBase
  • axon guidance Source: FlyBase
  • cell migration Source: GO_Central
  • cellularization Source: FlyBase
  • dorsal closure Source: FlyBase
  • epithelial cell-cell adhesion Source: FlyBase
  • female germline ring canal formation Source: FlyBase
  • female germline ring canal formation, actin assembly Source: FlyBase
  • female germline ring canal stabilization Source: FlyBase
  • filtration diaphragm assembly Source: FlyBase
  • germarium-derived egg chamber formation Source: FlyBase
  • germarium-derived female germ-line cyst encapsulation Source: FlyBase
  • innate immune response Source: GO_Central
  • JNK cascade Source: FlyBase
  • long-term memory Source: FlyBase
  • mushroom body development Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • oocyte karyosome formation Source: FlyBase
  • oogenesis Source: FlyBase
  • open tracheal system development Source: FlyBase
  • ovarian fusome organization Source: FlyBase
  • ovarian nurse cell to oocyte transport Source: FlyBase
  • parallel actin filament bundle assembly Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • positive regulation of protein kinase activity Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of actin polymerization or depolymerization Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of synaptic plasticity Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1994
ReactomeiR-DME-1227986 Signaling by ERBB2
R-DME-1295596 Spry regulation of FGF signaling
R-DME-1433557 Signaling by SCF-KIT
R-DME-177929 Signaling by EGFR
R-DME-180292 GAB1 signalosome
R-DME-2454202 Fc epsilon receptor (FCERI) signaling
R-DME-354192 Integrin alphaIIb beta3 signaling
R-DME-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-DME-372708 p130Cas linkage to MAPK signaling for integrins
R-DME-373753 Nephrin family interactions
R-DME-375165 NCAM signaling for neurite out-growth
R-DME-3928662 EPHB-mediated forward signaling
R-DME-3928663 EPHA-mediated growth cone collapse
R-DME-3928664 Ephrin signaling
R-DME-3928665 EPH-ephrin mediated repulsion of cells
R-DME-418592 ADP signalling through P2Y purinoceptor 1
R-DME-418885 DCC mediated attractive signaling
R-DME-430116 GP1b-IX-V activation signalling
R-DME-437239 Recycling pathway of L1
R-DME-4420097 VEGFA-VEGFR2 Pathway
R-DME-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-DME-5621575 CD209 (DC-SIGN) signaling
R-DME-5663220 RHO GTPases Activate Formins
R-DME-5673000 RAF activation
R-DME-5673001 RAF/MAP kinase cascade
R-DME-5674135 MAP2K and MAPK activation
R-DME-6798695 Neutrophil degranulation
R-DME-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-DME-8866376 Reelin signalling pathway
R-DME-8934903 Receptor Mediated Mitophagy
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-912631 Regulation of signaling by CBL
SignaLinkiP00528

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Src64B (EC:2.7.10.2)
Short name:
Dsrc64
Gene namesi
Name:Src64B
Synonyms:Src1
ORF Names:CG7524
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0262733 Src64B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881401 – 552Tyrosine-protein kinase Src64BAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei434Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00528
PRIDEiP00528

PTM databases

iPTMnetiP00528

Expressioni

Tissue specificityi

After the first 8 hours of development, accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal disks, and in differentiating smooth muscle.1 Publication

Developmental stagei

Abundant in embryos and pupae, rare in larvae and adults.1 Publication

Gene expression databases

BgeeiFBgn0262733
ExpressionAtlasiP00528 baseline and differential
GenevisibleiP00528 DM

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • signaling receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi71867, 65 interactors
DIPiDIP-17438N
IntActiP00528, 9 interactors
STRINGi7227.FBpp0293527

Structurei

3D structure databases

ProteinModelPortaliP00528
SMRiP00528
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 156SH3PROSITE-ProRule annotationAdd BLAST62
Domaini162 – 259SH2PROSITE-ProRule annotationAdd BLAST98
Domaini284 – 537Protein kinasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000118938
InParanoidiP00528
KOiK05704
OMAiGKWRNNI
OrthoDBiEOG091G0D46
PhylomeDBiP00528

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

P00528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNKCCSKRQ DQELALAYPT GGYKKSDYTF GQTHINSSGG GNMGGVLGQK
60 70 80 90 100
HNNGGSLDSR YTPDPNHRGP LKIGGKGGVD IIRPRTTPTG VPGVVLKRVV
110 120 130 140 150
VALYDYKSRD ESDLSFMKGD RMEVIDDTES DWWRVVNLTT RQEGLIPLNF
160 170 180 190 200
VAEERSVNSE DWFFENVLRK EADKLLLAEE NPRGTFLVRP SEHNPNGYSL
210 220 230 240 250
SVKDWEDGRG YHVKHYRIKP LDNGGYYIAT NQTFPSLQAL VMAYSKNALG
260 270 280 290 300
LCHILSRPCP KPQPQMWDLG PELRDKYEIP RSEIQLLRKL GRGNFGEVFY
310 320 330 340 350
GKWRNSIDVA VKTLREGTMS TAAFLQEAAI MKKFRHNRLV ALYAVCSQEE
360 370 380 390 400
PIYIVQEYMS KGSLLDFLRE GDGRYLHFED LIYIATQVAS GMEYLESKQL
410 420 430 440 450
IHRDLAARNV LIGENNVAKI CDFGLARVIA DDEYCPKQGS RFPVKWTAPE
460 470 480 490 500
AIIYGKFSIK SDVWSYGILL MELFTYGQVP YPGMHSREVI ENIERGFRMP
510 520 530 540 550
KPTNHYFPDN IYQLLLQCWD AVPEKRPTFE FLNHYFESFS VTSEVPYREV

QD
Length:552
Mass (Da):63,003
Last modified:June 1, 2001 - v3
Checksum:i4A63CF4F16562864
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102A → S in AAA28913 (PubMed:2996778).Curated1
Sequence conflicti261 – 272KPQPQ…DLGPE → ASLPQTAAPDVGFGPQ in AAA28489 (PubMed:6317185).CuratedAdd BLAST12
Sequence conflicti286 – 287LL → VV in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti290L → V in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti293G → R in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti316E → A in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti366D → N in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti373G → D in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti384 – 385IA → MH in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti389 – 390AS → TT in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti393E → K in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti400L → V in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti406 – 407AA → TT in AAA28489 (PubMed:6317185).Curated2
Sequence conflicti435C → R in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti460K → E in CAA05754 (PubMed:9731193).Curated1
Sequence conflicti471M → T in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti484M → L in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti507F → L in AAA28489 (PubMed:6317185).Curated1
Sequence conflicti536F → L in AAA28489 (PubMed:6317185).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11917 mRNA Translation: AAA28913.1
AE014296 Genomic DNA Translation: AAF47922.1
AE014296 Genomic DNA Translation: AAX52734.1
AE014296 Genomic DNA Translation: AAX52735.1
AE014296 Genomic DNA Translation: AAX52736.1
AE014296 Genomic DNA Translation: AAX52737.1
AY051781 mRNA Translation: AAK93205.1
K01043 Genomic DNA Translation: AAA28489.1
AJ002919 Genomic DNA Translation: CAA05754.1
RefSeqiNP_001014561.1, NM_001014561.2
NP_001014562.1, NM_001014562.3
NP_001014563.1, NM_001014563.3
NP_001014564.1, NM_001014564.3
NP_001189051.1, NM_001202122.1
NP_001246628.1, NM_001259699.2
NP_001246629.1, NM_001259700.2
NP_524934.2, NM_080195.4
UniGeneiDm.1531

Genome annotation databases

EnsemblMetazoaiFBtr0073321; FBpp0073177; FBgn0262733
FBtr0100504; FBpp0099944; FBgn0262733
FBtr0100505; FBpp0099946; FBgn0262733
FBtr0100507; FBpp0099947; FBgn0262733
FBtr0100508; FBpp0099948; FBgn0262733
FBtr0302594; FBpp0291750; FBgn0262733
FBtr0304989; FBpp0293526; FBgn0262733
FBtr0304990; FBpp0293527; FBgn0262733
GeneIDi48973
KEGGidme:Dmel_CG7524

Similar proteinsi

Entry informationi

Entry nameiSRC64_DROME
AccessioniPrimary (citable) accession number: P00528
Secondary accession number(s): A4V1H7, O18372, Q9VZA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 203 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health