P00527 (YES_AVISY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transforming protein Yes
EC=2.7.10.2

Gene names

Name:

V-YES

Organism

Avian sarcoma virus (strain Y73)

Taxonomic identifier

11884 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Alpharetrovirus

Virus host

Galliformes [TaxID: 8976]

Protein attributes

Sequence length	528 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Miscellaneous	This protein is synthesized as a Gag-Yes polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
Sequence caution	The sequence CAA24496.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Disease	Oncogene
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 528	›528	Tyrosine-protein kinase transforming protein Yes		PRO_0000088179
Regions
	Domain	81 – 142	62	SH3
	Domain	148 – 245	98	SH2
	Domain	267 – 520	254	Protein kinase
	Nucleotide binding	273 – 281	9	ATP By similarity
Sites
	Active site	386	1	Proton acceptor By similarity
	Binding site	295	1	ATP By similarity
Amino acid modifications
	Modified residue	416	1	Phosphotyrosine; by autocatalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P00527 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 98F748C8879D7709	FASTA	528	59,104
	10 20 30 40 50 60 DKGPAMKYRT DNTPEPISSH VSHYGSDSSQ ATQSPAIKGS AVNFNSHSMT PFGGPSGMTP 70 80 90 100 110 120 FGGASSSFSA VPSPYPSTLT GGGTVFVALY DYEARTTDDL SFKGGERFQI INNTEGDWWE 130 140 150 160 170 180 ARSIATGKTG YIPSNYVAPA DSIEAEEWYF GKMGRKDAER LLLNPGNQRG IFLVRESETT 190 200 210 220 230 240 KGAYSLSIRD WDEVRGDNVK HYKIRKLDNG GYYITTRAQF ESLQKLVKHS REHADGLCHK 250 260 270 280 290 300 LTTVCPTVKP QTQGLAKDAW EIPRESLRLE VKLGQGCFGE VWMGTWNGTT KVAIKTLKLG 310 320 330 340 350 360 TMMPEAFLQE AQIMKKLRHD KLVPLYAVVS EEPIYIVTEF MTKGSLLDFL KEGEGKFLKL 370 380 390 400 410 420 PQLVDMAAQI ADGMAYIERM NYIHRDLRAA NILVGDNLVC KIADFGLARL IEDNEYTARQ 430 440 450 460 470 480 GAKFPIKWTA PEAALYGRFT IKSDVWSFGI LLTELVTKGR VPYPGMVNRE VLEQVERGYR 490 500 510 520 MPCPQGCPES LHELMKLCWK KDPDERPTFE YIQSFLEDYF TAAEPSGY

References

[1]	"Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus." Kitamura N., Kitamura A., Toyoshima K., Hirayama Y., Yoshida M. Nature 297:205-208(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01170 Unassigned RNA. Translation: CAA24496.1. Different initiation.
PIR	TVFVG9. A00633.
3D structure databases
ProteinModelPortal	P00527.
SMR	P00527. Positions 84-525.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 600.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YES_AVISY

Accession

Primary (citable) accession number: P00527

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program