Tyrosine-protein kinase transforming protein Src - Avian sarcoma virus (strain rASV1441)

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P00525 (SRC_AVISR) Reviewed, UniProtKB/Swiss-Prot

Last modified July 27, 2011. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase transforming protein Src
EC=2.7.10.2

Alternative name(s):
pp60v-src
Short name=p60-Src
Short name=v-Src

Gene names

Name:

V-SRC

Organism

Avian sarcoma virus (strain rASV1441)

Taxonomic identifier

11894 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Alpharetrovirus › unclassified Alpharetrovirus

Virus host

Galliformes [TaxID: 8976]

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This phosphoprotein, required for both the initiation and the maintenance of neoplastic transformation, is a protein kinase that catalyzes the phosphorylation of tyrosine residues in vitro.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Post-translational modification	The phosphorylated form is termed pp60v-src. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Disease	Oncogene
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed; by host By similarity

Chain

2 – 526

525

Tyrosine-protein kinase transforming protein Src

PRO_0000088147

Regions

Domain

81 – 142

SH3

Domain

148 – 245

SH2

Domain

267 – 517

251

Protein kinase

Nucleotide binding

273 – 281

ATP By similarity

Sites

Active site

386

Proton acceptor By similarity

Binding site

295

ATP By similarity

Amino acid modifications

Modified residue

416

Phosphotyrosine; by autocatalysis Ref.2

Lipidation

N-myristoyl glycine; by host By similarity

Secondary structure

526

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P00525 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7DB3903F80233E49
Show »

FASTA

526

58,878

        10         20         30         40         50         60 
MGSSKSKPKD PSQRRCSLEP PDSTHHGGFP ASQTPNKTAA PDTHRTPSRS FGTVATEPKL 

        70         80         90        100        110        120 
FGGFNTSDTV TSPQRAGALA GGVTTFVALY DYESRTETDL SFKKGERLQI VNNTEGDWWL 

       130        140        150        160        170        180 
AHSLTTGQTG YIPSNYVAPS DSIQAEEWYF GKITRRESER LLLNPENPRG TFLVRESETT 

       190        200        210        220        230        240 
KGAYCLSVSD FDNAKGLNVK HYKIRKLDSG GFYITSRTQF SSLQQLVAYY SKHADGLCHR 

       250        260        270        280        290        300 
LTNVCPTSKP QTQGLAKDAW EIPRESLRLE VKLGQGCFGE VWMGTWNGTT RVAIKTLKPG 

       310        320        330        340        350        360 
TMSPEAFLQE AQVMKKLRHE KLVQLYAVVS EEPIYIVTEY MSKGSLLDFL KGEMGKYLRL 

       370        380        390        400        410        420 
PQLVDMAAQI ASGMAYVERM NYVHRDLRAA NILVGENLVC KVADFGLARL IEDNEYTARQ 

       430        440        450        460        470        480 
GAKFPIKWTA PEAALYGRFT IKSDVWSFGI LLTELTTKGR VPYPGMGNGE VLDRVERGYR 

       490        500        510        520 
MPCPPECPES LHDLMCQCWR RDPEERPTFE YLQAQLLPAC VLEVAE

« Hide

References

[1]	"DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src." Takeya T., Feldman R.A., Hanafusa H. J. Virol. 44:1-11(1982) [PubMed: 6292477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses." Neil J.C., Ghysdael J., Vogt P.K., Smart J.E. Nature 291:675-677(1981) [PubMed: 6264320] [Abstract] Cited for: PHOSPHORYLATION AT TYR-416.
[3]	"Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions." Feng S., Chen J.K., Yu H., Simon J.A., Schreiber S.L. Science 266:1241-1247(1994) [PubMed: 7526465] [Abstract] Cited for: STRUCTURE BY NMR OF 85-140.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K00928 Genomic RNA. Translation: AAA42565.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QWE	NMR	-	A	81-140	[»]
1QWF	NMR	-	A	81-140	[»]

ProteinModelPortal

P00525.

SMR

P00525. Positions 83-516.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

2.7.10.2. 600.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

Pfam

PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.

SMART

SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.
SSF50044. SH3. 1 hit.

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P00525.

Entry information

Entry name

SRC_AVISR

Accession

Primary (citable) accession number: P00525

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	July 27, 2011
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents