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Protein

Tyrosine-protein kinase transforming protein Src

Gene

V-SRC

Organism
Avian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This phosphoprotein, required for both the initiation and the maintenance of neoplastic transformation, is a protein kinase that catalyzes the phosphorylation of tyrosine residues in vitro.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295ATPPROSITE-ProRule annotation1
Active sitei386Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 281ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase transforming protein Src (EC:2.7.10.2)
Alternative name(s):
pp60v-src
Short name:
p60-Src
Short name:
v-Src
Gene namesi
Name:V-SRC
OrganismiAvian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A))
Taxonomic identifieri269446 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeAlpharetrovirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00000881532 – 526Tyrosine-protein kinase transforming protein SrcAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1
Modified residuei416Phosphotyrosine; by autocatalysis1 Publication1

Post-translational modificationi

The phosphorylated form is termed pp60v-src.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

PTM databases

iPTMnetiP00524.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP00524. 4 interactors.
MINTiMINT-215689.

Structurei

Secondary structure

1526
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi149 – 152Combined sources4
Helixi155 – 162Combined sources8
Beta strandi172 – 176Combined sources5
Beta strandi178 – 180Combined sources3
Beta strandi184 – 192Combined sources9
Turni193 – 195Combined sources3
Beta strandi196 – 206Combined sources11
Beta strandi208 – 210Combined sources3
Beta strandi212 – 215Combined sources4
Beta strandi219 – 222Combined sources4
Helixi223 – 230Combined sources8
Beta strandi237 – 239Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BKLX-ray2.10A145-249[»]
1BKMX-ray2.00A145-249[»]
1IS0X-ray1.90A/B144-249[»]
1KC2X-ray2.10A145-247[»]
1NZLX-ray1.90A/B145-247[»]
1NZVX-ray2.10A/B145-247[»]
1SHAX-ray1.50A144-247[»]
1SHBX-ray2.00A144-247[»]
1SKJX-ray2.00A145-249[»]
1SPRX-ray2.50A/B/C/D144-247[»]
1SPSX-ray2.70A/B/C144-247[»]
ProteinModelPortaliP00524.
SMRiP00524.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00524.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 142SH3PROSITE-ProRule annotationAdd BLAST62
Domaini148 – 245SH2PROSITE-ProRule annotationAdd BLAST98
Domaini267 – 517Protein kinasePROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSKSKPKD PSQRRCSLEP PDSTHHGGFP ASQTPNKTAA PDTHRTPSRS
60 70 80 90 100
FGTVATEPKL FGGFNTSDTV TSPQRAGALA GGVTTFVALY DYESRTETDL
110 120 130 140 150
SFKKGERLQI VNNTEGDWWL AHSLTTGQTG YIPSNYVAPS DSIQAEEWYF
160 170 180 190 200
GKITRRESER LLLNPENPRG TFLVRESETT KGAYCLSVSD FDNAKGLNVK
210 220 230 240 250
HYKIRKLDSG GFYITSRTQF SSLQQLVAYY SKHADGLCHR LTNVCPTSKP
260 270 280 290 300
QTQGLAKDAW EIPRESLRLE VKLGQGCFGE VWMGTWNGTT RVAIKTLKPG
310 320 330 340 350
TMSPEAFLQE AQVMKKLRHE KLVQLYAVVS EEPIYIVTEY MSKGSLLDFL
360 370 380 390 400
KGEMGKYLRL PQLVDMAAQI ASGMAYVERM NYVHRDLRAA NILVGENLVC
410 420 430 440 450
KVADFGLARL IEDNEYTARQ GAKFPIKWTA PEAALYGRFT IKSDVWSFGI
460 470 480 490 500
LLTELTTKGR VPYPGMGNGE VLDRVERGYR MPCPPECPES LHDLMCQCWR
510 520
RDPEERPTFE YLQAQLLPAC VLEVAE
Length:526
Mass (Da):58,878
Last modified:January 23, 2007 - v5
Checksum:i7DB3903F80233E49
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16C → R (PubMed:6253794).Curated1
Sequence conflicti16C → R (PubMed:6298633).Curated1
Sequence conflicti95 – 96RT → WI (PubMed:6253794).Curated2
Sequence conflicti95 – 96RT → WI (PubMed:6298633).Curated2
Sequence conflicti117D → N (PubMed:6253794).Curated1
Sequence conflicti117D → N (PubMed:6298633).Curated1
Sequence conflicti338T → I (PubMed:6253794).Curated1
Sequence conflicti338T → I (PubMed:6298633).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29199 Genomic DNA. Translation: AAA42563.1.
V01169 Genomic RNA. Translation: CAA24495.1.
K00928 Genomic RNA. Translation: AAA42565.1.
PIRiA38017. TVFV60.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29199 Genomic DNA. Translation: AAA42563.1.
V01169 Genomic RNA. Translation: CAA24495.1.
K00928 Genomic RNA. Translation: AAA42565.1.
PIRiA38017. TVFV60.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BKLX-ray2.10A145-249[»]
1BKMX-ray2.00A145-249[»]
1IS0X-ray1.90A/B144-249[»]
1KC2X-ray2.10A145-247[»]
1NZLX-ray1.90A/B145-247[»]
1NZVX-ray2.10A/B145-247[»]
1SHAX-ray1.50A144-247[»]
1SHBX-ray2.00A144-247[»]
1SKJX-ray2.00A145-249[»]
1SPRX-ray2.50A/B/C/D144-247[»]
1SPSX-ray2.70A/B/C144-247[»]
ProteinModelPortaliP00524.
SMRiP00524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00524. 4 interactors.
MINTiMINT-215689.

PTM databases

iPTMnetiP00524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP00524.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRC_RSVSA
AccessioniPrimary (citable) accession number: P00524
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 146 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.