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Protein

Tyrosine-protein kinase Abl

Gene

Abl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Arm and Abl proteins function cooperatively at adherens junctions in both the CNS and epidermis; critical for embryonic epithelial morphogenesis regulating cell shape changes and cell migration. Plays a critical role in transducing embryonic midline repulsive cues; may regulate cytoskeletal dynamics underlying a growth cone's response to midline cues. The ability of pCC/MP2 axons to correctly interpret midline repulsive cues and stay on the ipsilateral side is dependent on the strength of both Slit/robo and Abl-dependent signaling pathways.3 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei400ATPPROSITE-ProRule annotation1
Active sitei492Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi377 – 385ATPPROSITE-ProRule annotation9
Nucleotide bindingi445 – 451ATPPROSITE-ProRule annotation7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • non-membrane spanning protein tyrosine kinase activity Source: FlyBase
  • protein tyrosine kinase activity Source: FlyBase
  • signaling receptor binding Source: GO_Central

GO - Biological processi

  • axis elongation Source: FlyBase
  • axon guidance Source: FlyBase
  • axon midline choice point recognition Source: FlyBase
  • axonogenesis Source: FlyBase
  • branchiomotor neuron axon guidance Source: CACAO
  • central nervous system development Source: FlyBase
  • chemical synaptic transmission Source: CACAO
  • compound eye development Source: FlyBase
  • cytoplasmic transport, nurse cell to oocyte Source: FlyBase
  • dendrite morphogenesis Source: FlyBase
  • dorsal closure Source: FlyBase
  • epithelial cell morphogenesis Source: FlyBase
  • innate immune response Source: GO_Central
  • learning or memory Source: CACAO
  • morphogenesis of an epithelium Source: FlyBase
  • motor neuron axon guidance Source: FlyBase
  • negative regulation of neuron projection development Source: CACAO
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • neuron migration Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • photoreceptor cell axon guidance Source: FlyBase
  • positive regulation of lamellipodium assembly Source: CACAO
  • positive regulation of protein export from nucleus Source: FlyBase
  • protein autophosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of actin polymerization or depolymerization Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of cell shape Source: FlyBase
  • regulation of protein localization Source: FlyBase
  • regulation of protein stability Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
  • ventral cord development Source: FlyBase
  • ventral furrow formation Source: FlyBase

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1994
ReactomeiR-DME-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DME-375170 CDO in myogenesis
R-DME-428890 Role of ABL in ROBO-SLIT signaling
R-DME-5663213 RHO GTPases Activate WASPs and WAVEs
R-DME-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-DME-8939236 RUNX1 regulates transcription of genes involved in differentiation of HSCs
SignaLinkiP00522

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Abl (EC:2.7.10.2)
Alternative name(s):
D-ash
Protein abelson
Gene namesi
Name:Abl
Synonyms:ABL-1, Dash
ORF Names:CG4032
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000017 Abl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Both loss- and gain-of-function mutants exhibit neurons within the pCC/MP2 pathway that incorrectly project across the midline. Loss of Abl disrupts cell migration and cell shape changes during dorsal closure.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880541 – 1620Tyrosine-protein kinase AblAdd BLAST1620

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei522Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1497Phosphotyrosine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP00522
PRIDEiP00522

PTM databases

iPTMnetiP00522

Expressioni

Tissue specificityi

Arm and ena colocalize with Abl at adherens junctions throughout development.2 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0000017
ExpressionAtlasiP00522 baseline and differential
GenevisibleiP00522 DM

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi69904, 40 interactors
IntActiP00522, 8 interactors
STRINGi7227.FBpp0303166

Structurei

3D structure databases

ProteinModelPortaliP00522
SMRiP00522
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 248SH3PROSITE-ProRule annotationAdd BLAST62
Domaini254 – 346SH2PROSITE-ProRule annotationAdd BLAST93
Domaini371 – 627Protein kinasePROSITE-ProRule annotationAdd BLAST257

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi510 – 534Kinase activation loopBy similarityAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 92Gly/Ser-richAdd BLAST91
Compositional biasi1056 – 1080Pro-richAdd BLAST25

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG4278 Eukaryota
COG0515 LUCA
InParanoidiP00522
KOiK06619
OMAiNMFQESS
OrthoDBiEOG091G0D46

Family and domain databases

CDDicd09935 SH2_ABL, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR033221 ABL1
IPR035837 ABL_SH2
IPR015015 F-actin_binding
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PANTHERiPTHR24418:SF88 PTHR24418:SF88, 1 hit
PfamiView protein in Pfam
PF08919 F_actin_bind, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00808 FABD, 1 hit
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

P00522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAQQGKDRG AHSGGGGSGA PVSCIGLSSS PVASVSPHCI SSSSGVSSAP
60 70 80 90 100
LGGGSTLRGS RIKSSSSGVA SGSGSGGGGG GSGSGLSQRS GGHKDARCNP
110 120 130 140 150
TVGLNIFTEH NEALLQSRPL PHIPAGSTAA SLLADAAELQ QHQQDSGGLG
160 170 180 190 200
LQGSSLGGGH SSTTSVFESA HRWTSKENLL APGPEEDDPQ LFVALYDFQA
210 220 230 240 250
GGENQLSLKK GEQVRILSYN KSGEWCEAHS DSGNVGWVPS NYVTPLNSLE
260 270 280 290 300
KHSWYHGPIS RNAAEYLLSS GINGSFLVRE SESSPGQRSI SLRYEGRVYH
310 320 330 340 350
YRISEDPDGK VFVTQEAKFN TLAELVHHHS VPHEGHGLIT PLLYPAPKQN
360 370 380 390 400
KPTVFPLSPE PDEWEICRTD IMMKHKLGGG QYGEVYEAVW KRYGNTVAVK
410 420 430 440 450
TLKEDTMALK DFLEEAAIMK EMKHPNLVQL IGVCTREPPF YIITEFMSHG
460 470 480 490 500
NLLDFLRSAG RETLDAVALL YMATQIASGM SYLESRNYIH RDLAARNCLV
510 520 530 540 550
GDNKLVKVAD FGLARLMRDD TYTAHAGAKF PIKWTAPEGL AYNKFSTKSD
560 570 580 590 600
VWAFGVLLWE IATYGMSPYP AIDLTDVYHK LDKGYRMERP PGCPPEVYDL
610 620 630 640 650
MRQCWQWDAT DRPTFKSIHH ALEHMFQESS ITEAVEKQLN ANATSASSSA
660 670 680 690 700
PSTSGVATGG GATTTTAASG CASSSSATAS LSLTPQMVKK GLPGGQALTP
710 720 730 740 750
NAHHNDPHQQ QASTPMSETG STSTKLSTFS SQGKGNVQMR RTTNKQGKQA
760 770 780 790 800
PAPPKRTSLL SSSRDSTYRE EDPANARCNF IDDLSTNGLA RDINSLTQRY
810 820 830 840 850
DSETDPAADP DTDATGDSLE QSLSQVIAAP VTNKMQHSLH SGGGGGGIGP
860 870 880 890 900
RSSQQHSSFK RPTGTPVMGN RGLETRQSKR SQLHSQAPGP GPPSTQPHHG
910 920 930 940 950
NNGVVTSAHP ITVGALDVMN VKQVVNRYGT LPKGARIGAY LDSLEDSSEA
960 970 980 990 1000
APALPATAPS LPPANGHATP PAARLNPKAS PIPPQQMIRS NSSGGVTMQN
1010 1020 1030 1040 1050
NAAASLNKLQ RHRTTTEGTM MTFSSFRAGG SSSSPKRSAS GVASGVQPAL
1060 1070 1080 1090 1100
ANLEFPPPPL DLPPPPEEFE GGPPPPPPAP ESAVQAIQQH LHAQLPNNGN
1110 1120 1130 1140 1150
ISNGNGTNNN DSSHNDVSNI APSVEEASSR FGVSLRKREP STDSCSSLGS
1160 1170 1180 1190 1200
PPEDLKEKLI TEIKAAGKDT APASHLANGS GIAVVDPVSL LVTELAESMN
1210 1220 1230 1240 1250
LPKPPPQQQQ KLTNGNSTGS GFKAQLKKVE PKKMSAPMPK AEPANTIIDF
1260 1270 1280 1290 1300
KAHLRRVDKE KEPATPAPAP ATVAVANNAN CNTTGTLNRK EDGSKKFSQA
1310 1320 1330 1340 1350
MQKTEIKIDV TNSNVEADAG AAGEGDLGKR RSTGSINSLK KLWEQQPPAP
1360 1370 1380 1390 1400
DYATSTILQQ QPSVVNGGGT PNAQLSPKYG MKSGAINTVG TLPAKLGNKQ
1410 1420 1430 1440 1450
PPAAPPPPPP NCTTSNSSTT SISTSSRDCT SRQQASSTIK TSHSTQLFTD
1460 1470 1480 1490 1500
DEEQSHTEGL GSGGQGSADM TQSLYEQKPQ IQQKPAVPHK PTKLTIYATP
1510 1520 1530 1540 1550
IAKLTEPASS ASSTQISRES ILELVGLLEG SLKHPVNAIA GSQWLQLSDK
1560 1570 1580 1590 1600
LNILHNSCVI FAENGAMPPH SKFQFRELVT RVEAQSQHLR SAGSKNVQDN
1610 1620
ERLVAEVGQS LRQISNALNR
Length:1,620
Mass (Da):171,588
Last modified:April 12, 2005 - v3
Checksum:i14287B02CC8FE86B
GO

Sequence cautioni

The sequence AAA28934 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAL13726 differs from that shown. Reason: Frameshift at position 323.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129 – 136AASLLADA → RPLFWRI in AAA28934 (PubMed:2832740).Curated8
Sequence conflicti357 – 360LSPE → ASAQ (PubMed:6317185).Curated4
Sequence conflicti628 – 631ESSI → VGDV (PubMed:6317185).Curated4
Sequence conflicti1241 – 1243AEP → RT in AAA28934 (PubMed:2832740).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19692, M19690, M19691 Genomic DNA Translation: AAA28934.1 Sequence problems.
AE014296 Genomic DNA Translation: AAF49431.2
AY058497 mRNA Translation: AAL13726.1 Frameshift.
K01042 Genomic DNA Translation: AAA28443.1
PIRiA28128 TVFFA
RefSeqiNP_001287085.1, NM_001300156.1
NP_524843.2, NM_080104.3
UniGeneiDm.5397

Genome annotation databases

EnsemblMetazoaiFBtr0075357; FBpp0075116; FBgn0000017
FBtr0345369; FBpp0311523; FBgn0000017
GeneIDi45821
KEGGidme:Dmel_CG4032

Similar proteinsi

Entry informationi

Entry nameiABL_DROME
AccessioniPrimary (citable) accession number: P00522
Secondary accession number(s): Q95TV1, Q9VV86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 12, 2005
Last modified: May 23, 2018
This is version 195 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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