Reviewed, UniProtKB/Swiss-Prot P00521 (ABL_MLVAB)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase transforming protein Abl
    EC=2.7.10.2
Alternative name(s):
    V-abl

Gene names

Name:

ABL

Organism

Abelson murine leukemia virus

Taxonomic identifier

11788 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Miscellaneous	This protein is synthesized as a Gag-Abl polyprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Disease	Oncogene
Domain	SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TUSC2	O75896	1	EBI-1550592,EBI-1052725	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 746	746	Tyrosine-protein kinase transforming protein Abl		PRO_0000088057
Regions
	Domain	13 – 103	91	SH2
	Domain	128 – 379	252	Protein kinase
	Nucleotide binding	134 – 142	9	ATP By similarity
	Nucleotide binding	202 – 208	7	ATP By similarity
	Motif	267 – 291	25	Kinase activation loop By similarity
Sites
	Active site	249	1	Proton acceptor By similarity
	Binding site	157	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P00521-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: B9072FFF55FE9257	FASTA	746	81,872
	10 20 30 40 50 60 YITPVNSLEK HSWYHGPVSR NAAEYLLSSG INGSFLVRES ESSPGQRSIS LRYEGRVYHY 70 80 90 100 110 120 RINTASDGKL YVSSESRFNT LAELVHHHST VADGLITTLH YPAPKRNKPT IYGVSPNYDK 130 140 150 160 170 180 WEMERTDITM KHKLGGGQYG EVYEGVWKKY SLTVAVKTLK EDTMEVEEFL KEAAVMKEIK 190 200 210 220 230 240 HPNLVQLLGV CTREPPFYII TEFMTYGNLL DYLRECNRQE VSAVVLLYMA TQISSAMEYL 250 260 270 280 290 300 EKKNFIHRDL AARNCLVGEN HLVKVADFGL SRLMTGDTYT AHAGAKFPIK WTAPESLAYN 310 320 330 340 350 360 KFSIKSDVWA FGVLLWEIAT YGMSPYPGID LSQVYELLEK DYRMERPEGC PEKVYELMRA 370 380 390 400 410 420 CWQWNPSDRP SFAEIHQAFE TMFQESSISD EVEKELGKRG TRGGAGSMLQ APELPTKTRT 430 440 450 460 470 480 CRRAAEQKAS PPSLTPKLLR RQVTASPSSG LSHKKEATKG SASGMGTPAT AEPAPPSNKV 490 500 510 520 530 540 GLSKASSEEM RVRRHKHSSE SPGRDKGRLA KLKPAPPPPP ACTGKAGKPA QSPSQEAGEA 550 560 570 580 590 600 GGPTKTKCTS LAMDAVNTDP TKAGPPGEGL RKPVPPSVPK PQSTAKPPGT PTSPVSTPST 610 620 630 640 650 660 APAPSPLAGD QQPSSAAFIP LISTRVSLRK TRQPPERIAS GTITKGVVLD STEALCLAIS 670 680 690 700 710 720 RNSEQMASHS AVLEAGKNLY TFCVSYVDSI QQMRNKFAFR EAINKLESNL RELQICPATA 730 740 SSGPAATQDF SKLLSSVKEI SDIVRR

References

[1]	"Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity." Reddy E.P., Smith M.J., Srinivasan A. Proc. Natl. Acad. Sci. U.S.A. 80:3623-3627(1983) [PubMed: 6304726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	Erratum Reddy E.P., Smith M.J., Srinivasan A. Proc. Natl. Acad. Sci. U.S.A. 80:7372-7372(1983) Cited for: SEQUENCE REVISION TO 588-746.
[3]	"Homology between phosphotyrosine acceptor site of human c-abl and viral oncogene products." Groffen J., Heisterkamp N., Reynolds F.H. Jr., Stephenson J.R. Nature 304:167-169(1983) [PubMed: 6191223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 233-327.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01541 Genomic DNA. No translation available.
K00010 Genomic RNA. Translation: AAA46470.1.

RefSeq

NP_955595.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3KF4	X-ray	1.90	A/B	115-401	[»]
3KFA	X-ray	1.22	A/B	115-401	[»]

SMR

P00521. Positions 1-398, 640-746.

ModBase

Search...

Protein-protein interaction databases

IntAct

P00521. 1 interaction.

Genome annotation databases

GeneID

1491873.

Enzyme and pathway databases

BRENDA

2.7.10.2. 281698.

Family and domain databases

InterPro

IPR015015. F-actin_binding.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR020700. Tyr_kinase_non-rcpt_Abl.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
[Graphical view]

Gene3D

G3DSA:3.30.505.10. SH2. 1 hit.

PANTHER

PTHR23256:SF144. Tyr_kinase_non-rcpt_Abl. 1 hit.
PTHR23256. Tyr_prot_kinase. 1 hit.

Pfam

PF08919. F_actin_bind. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]

PRINTS

PR00401. SH2DOMAIN.

SMART

SM00808. FABD. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ABL_MLVAB

Accession

Primary (citable) accession number: P00521

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)