Reviewed, UniProtKB/Swiss-Prot P00516 (KGP1A_BOVIN)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1, alpha isozyme
      Short name=CGK 1 alpha
      Short name=cGKI-alpha
    EC=2.7.11.12

Gene names

Name:	PRKG1
Synonyms:	PRKGR1A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation.
Subunit structure	Homodimer, antiparallel and monomer.
Post-translational modification	65 kDa monomer is produced by proteolytic cleavage.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Disulfide bond Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CGK1-alpha (identifier: P00516-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CGK1-beta (identifier: P21136-1)

The sequence of this isoform can be found in the external entry P21136-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 671	670	cGMP-dependent protein kinase 1, alpha isozyme		PRO_0000086114
Regions
	Domain	360 – 619	260	Protein kinase
	Domain	620 – 671	52	AGC-kinase C-terminal
	Nucleotide binding	103 – 220	118	cGMP 1
	Nucleotide binding	221 – 341	121	cGMP 2
	Nucleotide binding	366 – 374	9	ATP By similarity
	Region	2 – 102	101	Dimerization
Sites
	Active site	484	1	Proton acceptor By similarity
	Binding site	390	1	ATP
	Site	78 – 79	2	Cleavage
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.2
	Modified residue	59	1	Phosphothreonine; by autocatalysis Ref.3
	Disulfide bond	43		Interchain

Sequences

	Sequence		Length	Mass (Da)
	Isoform CGK1-alpha [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9FE7A48422DA9CDF	FASTA	671	76,419
	10 20 30 40 50 60 MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP STHIGPRTTR 70 80 90 100 110 120 AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD NDFMKNLELS QIQEIVDCMY 130 140 150 160 170 180 PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV TKEGVKLCTM GPGKVFGELA ILYNCTRTAT 190 200 210 220 230 240 VKTLVNVKLW AIDRQCFQTI MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET 250 260 270 280 290 300 HYENGEYIIR QGARGDTFFI ISKGKVNVTR EDSPNEDPVF LRTLGKGDWF GEKALQGEDV 310 320 330 340 350 360 RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA FFANLKLSDF 370 380 390 400 410 420 NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT RQQEHIRSEK QIMQGAHSDF 430 440 450 460 470 480 IVRLYRTFKD SKYLYMLMEA CLGGELWTIL RDRGSFEDST TRFYTACVVE AFAYLHSKGI 490 500 510 520 530 540 IYRDLKPENL ILDHRGYAKL VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD 550 560 570 580 590 600 YWSLGILMYE LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS 610 620 630 640 650 660 ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD SFPEDNDEPP 670 PDDNSGWDID F
	Isoform CGK1-beta. See P21136.	FASTA	–	–

References

[1]	"The cDNA of the two isoforms of bovine cGMP-dependent protein kinase." Wernet W., Flockerzi V., Hofmann F. FEBS Lett. 251:191-196(1989) [PubMed: 2546820] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Guanosine cyclic 3',5'-phosphate dependent protein kinase, a chimeric protein homologous with two separate protein families." Takio K., Wade R.D., Smith S.B., Krebs E.G., Walsh K.A., Titani K. Biochemistry 23:4207-4218(1984) [PubMed: 6091741] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-18; 90-375 AND 408-671, ACETYLATION AT SER-2.
[3]	"Amino acid sequence around a 'hinge' region and its 'autophosphorylation' site in bovine lung cGMP-dependent protein kinase." Takio K., Smith S.B., Walsh K.A., Krebs E.G., Titani K. J. Biol. Chem. 258:5531-5536(1983) [PubMed: 6304091] [Abstract] Cited for: PROTEIN SEQUENCE OF 14-105, PHOSPHORYLATION AT THR-59, BLOCKED N-TERMINUS.
[4]	"Amino acid sequence at the ATP-binding site of cGMP-dependent protein kinase." Hashimoto E., Takio K., Krebs E.G. J. Biol. Chem. 257:727-733(1982) [PubMed: 6274862] [Abstract] Cited for: PROTEIN SEQUENCE OF 374-410, ATP BINDING AT LYS-390.
[5]	"A catalytically active fragment of cGMP-dependent protein kinase. Occupation of its cGMP-binding sites does not affect its phosphotransferase activity." Heil W.G., Landgraf W., Hofmann F. Eur. J. Biochem. 168:117-121(1987) [PubMed: 2822399] [Abstract] Cited for: PROTEIN SEQUENCE OF 79-82, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16086 mRNA. Translation: CAA34214.1.
IPI	IPI00713131.
PIR	OKBOG. A00619.
RefSeq	NP_776861.1.
UniGene	Bt.63035 Bt.9
3D structure databases
HSSP	HSSP built from PDB template 1ATP based on UniProtKB P05132.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000018404. Bos taurus. [Contig view]
GeneID	282004.
KEGG	bta:282004.
Phylogenomic databases
HOVERGEN	P00516.
Enzyme and pathway databases
BRENDA	2.7.11.12. 251.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR018488. cNMP_bd_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGP1A_BOVIN

Accession

Primary (citable) accession number: P00516

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)