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Protein

Protein kinase 0.7

Gene

0.7

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase that modulates host metabolism to favor virus replication cycle. Participates together with gene 2 protein (gp2) in the host transcription shutoff. Phosphorylates host RNA polymerase subunit RpoC and to a lesser extent subunit RpoB. This modification seems to increase rho-dependent transcription termination, which may help to switch from host to viral RNA polymerase transcription during phage development. Phosphorylates host components of the RNA degradosome rne and RhlB, leading to stabilization of mRNAs synthesized by T7 RNA polymerase. Phosphorylates host dsRNA specific processing enzyme RNase III/rnc, leading to enhance activity of the ribonuclease that may increase maturation of T7 late transcripts possessing multiple Rnase III processing sites. Phosphorylates several components of the host translational machinery including initiation factors InfA, InfB, and InfC, ribosomal subunit RpsA and RpsF, as well as elongation factor G/FusA. These phosphorylations may enhance T7 late protein production.6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processBacterial host gene expression shutoff by virus, Bacterial host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase 0.7 (EC:2.7.11.1)
Alternative name(s):
Gene product 0.7
Short name:
Gp0.7
Protein kinase gp0.7
Gene namesi
Ordered Locus Names:0.7
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeAutographivirinaeT7virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001064651 – 359Protein kinase 0.7Add BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216Phosphoserine; by autocatalysis1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP00513.

Interactioni

Protein-protein interaction databases

IntActiP00513. 1 interactor.
MINTiMINT-1513843.

Structurei

3D structure databases

SMRiP00513.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili231 – 283Sequence analysisAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi248 – 296Arg-richAdd BLAST49

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG090002I2.

Family and domain databases

InterProiView protein in InterPro
IPR014374. Protein_kinase_T7-type.
PIRSFiPIRSF000547. Prot_kinase_T7. 1 hit.

Sequencei

Sequence statusi: Complete.

P00513-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNITDIMNAI DAIKALPICE LDKRQGMLID LLVEMVNSET CDGELTELNQ
60 70 80 90 100
ALEHQDWWTT LKCLTADAGF KMLGNGHFSA AYSHPLLPNR VIKVGFKKED
110 120 130 140 150
SGAAYTAFCR MYQGRPGIPN VYDVQRHAGC YTVVLDALKD CERFNNDAHY
160 170 180 190 200
KYAEIASDII DCNSDEHDEL TGWDGEFVET CKLIRKFFEG IASFDMHSGN
210 220 230 240 250
IMFSNGDVPY ITDPVSFSQK KDGGAFSIDP EELIKEVEEV ARQKEIDRAK
260 270 280 290 300
ARKERHEGRL EARRFKRRNR KARKAHKAKR ERMLAAWRWA ERQERRNHEV
310 320 330 340 350
AVDVLGRTNN AMLWVNMFSG DFKALEERIA LHWRNADRMA IANGLTLNID

KQLDAMLMG
Length:359
Mass (Da):41,124
Last modified:July 21, 1986 - v1
Checksum:i029457CA245B4308
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24389.1.
V01127 Genomic DNA. Translation: CAA24332.1.
PIRiD94615. KIBPO7.
RefSeqiNP_041959.1. NC_001604.1.

Genome annotation databases

GeneIDi1261070.
KEGGivg:1261070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA. Translation: CAA24389.1.
V01127 Genomic DNA. Translation: CAA24332.1.
PIRiD94615. KIBPO7.
RefSeqiNP_041959.1. NC_001604.1.

3D structure databases

SMRiP00513.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00513. 1 interactor.
MINTiMINT-1513843.

PTM databases

iPTMnetiP00513.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261070.
KEGGivg:1261070.

Phylogenomic databases

OrthoDBiVOG090002I2.

Family and domain databases

InterProiView protein in InterPro
IPR014374. Protein_kinase_T7-type.
PIRSFiPIRSF000547. Prot_kinase_T7. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPK_BPT7
AccessioniPrimary (citable) accession number: P00513
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 15, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.