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Protein

Aspartate aminotransferase, mitochondrial

Gene

Got2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity1 Publication

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.1 Publication
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.1 Publication

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
Binding sitei162 – 1621SubstrateBy similarity
Binding sitei215 – 2151SubstrateBy similarity
Binding sitei407 – 4071SubstrateBy similarity

GO - Molecular functioni

  • amino acid binding Source: RGD
  • carboxylic acid binding Source: RGD
  • enzyme binding Source: RGD
  • kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
  • L-aspartate:2-oxoglutarate aminotransferase activity Source: UniProtKB
  • L-phenylalanine:2-oxoglutarate aminotransferase activity Source: UniProtKB-EC
  • phospholipid binding Source: RGD
  • poly(A) RNA binding Source: Ensembl
  • protein homodimerization activity Source: RGD
  • pyridoxal phosphate binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15069.
BRENDAi2.6.1.1. 5301.
ReactomeiR-RNO-70263. Gluconeogenesis.
R-RNO-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKP00507.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:Got2
Synonyms:Maat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi2722. Got2.

Subcellular locationi

  • Mitochondrion matrix 1 Publication
  • Cell membrane 1 Publication

  • Note: Located in the mitochondria of liver, pancreas, spleen, heart, pituitary gland and submandibular gland cells. In kidney, located in the mitochondria, on the cell surface of regions with protrusions in distal tubules, on the apical cell surface of protrusions along the microvilli in cortical collecting ducts, in condensing vacuoles, on the cell surface at cell boundaries of adjoining kidney cells and on the cell surface of endothelial cells lining capillaries in the glomerulus. Also located at the cell surface of endothelial cells lining arterioles and on the cell surface of lymphocytes.

GO - Cellular componenti

  • cell surface Source: RGD
  • extracellular exosome Source: Ensembl
  • mitochondrial inner membrane Source: RGD
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
  • myelin sheath Source: Ensembl
  • perikaryon Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2351.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929Mitochondrion4 PublicationsAdd
BLAST
Chaini30 – 430401Aspartate aminotransferase, mitochondrialPRO_0000001218Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphothreonineBy similarity
Modified residuei59 – 591N6-acetyllysineBy similarity
Modified residuei73 – 731N6-acetyllysine; alternateBy similarity
Modified residuei73 – 731N6-succinyllysine; alternateBy similarity
Modified residuei82 – 821N6-acetyllysineBy similarity
Modified residuei90 – 901N6-acetyllysine; alternateBy similarity
Modified residuei90 – 901N6-succinyllysine; alternateBy similarity
Modified residuei96 – 961Nitrated tyrosine; alternateBy similarity
Modified residuei96 – 961Phosphotyrosine; alternateBy similarity
Modified residuei107 – 1071N6-acetyllysine; alternateBy similarity
Modified residuei107 – 1071N6-succinyllysine; alternateBy similarity
Modified residuei122 – 1221N6-acetyllysine; alternateBy similarity
Modified residuei122 – 1221N6-succinyllysine; alternateBy similarity
Modified residuei143 – 1431PhosphoserineBy similarity
Modified residuei159 – 1591N6-acetyllysine; alternateBy similarity
Modified residuei159 – 1591N6-succinyllysine; alternateBy similarity
Modified residuei185 – 1851N6-acetyllysine; alternateBy similarity
Modified residuei185 – 1851N6-succinyllysine; alternateBy similarity
Modified residuei227 – 2271N6-succinyllysineBy similarity
Modified residuei234 – 2341N6-acetyllysineBy similarity
Modified residuei279 – 2791N6-(pyridoxal phosphate)lysine; alternate
Modified residuei279 – 2791N6-acetyllysine; alternateBy similarity
Modified residuei296 – 2961N6-acetyllysine; alternateBy similarity
Modified residuei296 – 2961N6-succinyllysine; alternateBy similarity
Modified residuei302 – 3021N6-acetyllysineBy similarity
Modified residuei309 – 3091N6-acetyllysine; alternateBy similarity
Modified residuei309 – 3091N6-succinyllysine; alternateBy similarity
Modified residuei338 – 3381N6-acetyllysine; alternateBy similarity
Modified residuei338 – 3381N6-succinyllysine; alternateBy similarity
Modified residuei345 – 3451N6-acetyllysineBy similarity
Modified residuei363 – 3631N6-acetyllysine; alternateBy similarity
Modified residuei363 – 3631N6-succinyllysine; alternateBy similarity
Modified residuei364 – 3641N6-acetyllysineBy similarity
Modified residuei387 – 3871N6-acetyllysineBy similarity
Modified residuei396 – 3961N6-acetyllysine; alternateBy similarity
Modified residuei396 – 3961N6-succinyllysine; alternateBy similarity
Modified residuei404 – 4041N6-acetyllysine; alternateBy similarity
Modified residuei404 – 4041N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP00507.
PRIDEiP00507.

PTM databases

iPTMnetiP00507.
PhosphoSiteiP00507.

Expressioni

Tissue specificityi

Expressed in all tissues tested: liver, pancreas, kidney, heart, spleen, arterioles, and lymphocytes.1 Publication

Gene expression databases

BgeeiENSRNOG00000011782.
GenevisibleiP00507. RN.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP00507. 2 interactions.
STRINGi10116.ENSRNOP00000015956.

Structurei

3D structure databases

ProteinModelPortaliP00507.
SMRiP00507. Positions 30-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00507.
KOiK14455.
OMAiWANHAAV.
OrthoDBiEOG091G06G3.
PhylomeDBiP00507.
TreeFamiTF300641.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLHSGRVL SGMAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAGK NLDKEYLPIG
110 120 130 140 150
GLADFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRV GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FSGALEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEMA AVVKKKNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPLYSNPP LNGARIAATI LTSPDLRKQW LQEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHITDQIGM FCFTGLKPEQ VERLTKEFSV
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Length:430
Mass (Da):47,314
Last modified:July 1, 1989 - v2
Checksum:iEDC8B862A20DB736
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301S → R in AAC13868 (PubMed:7759512).Curated
Sequence conflicti162 – 1621W → G AA sequence (PubMed:7470110).Curated
Sequence conflicti162 – 1621W → G AA sequence (PubMed:7309704).Curated
Sequence conflicti167 – 1693PIF → EIA AA sequence (PubMed:7470110).Curated
Sequence conflicti167 – 1693PIF → EIA AA sequence (PubMed:7309704).Curated
Sequence conflicti177 – 1771Q → E AA sequence (PubMed:7470110).Curated
Sequence conflicti177 – 1771Q → E AA sequence (PubMed:7309704).Curated
Sequence conflicti232 – 2321V → Y AA sequence (PubMed:7470110).Curated
Sequence conflicti232 – 2321V → Y AA sequence (PubMed:7309704).Curated
Sequence conflicti255 – 2551D → N AA sequence (PubMed:7470110).Curated
Sequence conflicti255 – 2551D → N AA sequence (PubMed:7309704).Curated
Sequence conflicti338 – 3392KQ → QG AA sequence (PubMed:7470110).Curated
Sequence conflicti338 – 3392KQ → QG AA sequence (PubMed:7309704).Curated
Sequence conflicti352 – 3521I → G AA sequence (PubMed:7470110).Curated
Sequence conflicti352 – 3521I → G AA sequence (PubMed:7309704).Curated
Sequence conflicti386 – 3861L → I AA sequence (PubMed:7470110).Curated
Sequence conflicti386 – 3861L → I AA sequence (PubMed:7309704).Curated
Sequence conflicti400 – 4001V → I AA sequence (PubMed:7470110).Curated
Sequence conflicti400 – 4001V → I AA sequence (PubMed:7309704).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18467 mRNA. Translation: AAB54275.1.
BC061792 mRNA. Translation: AAH61792.1.
U21158 Genomic DNA. Translation: AAC13868.1.
M12709 mRNA. Translation: AAA41267.1.
PIRiA28005. XNRTDM.
I55427.
RefSeqiNP_037309.1. NM_013177.2.
UniGeneiRn.98650.

Genome annotation databases

EnsembliENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782.
GeneIDi25721.
KEGGirno:25721.
UCSCiRGD:2722. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18467 mRNA. Translation: AAB54275.1.
BC061792 mRNA. Translation: AAH61792.1.
U21158 Genomic DNA. Translation: AAC13868.1.
M12709 mRNA. Translation: AAA41267.1.
PIRiA28005. XNRTDM.
I55427.
RefSeqiNP_037309.1. NM_013177.2.
UniGeneiRn.98650.

3D structure databases

ProteinModelPortaliP00507.
SMRiP00507. Positions 30-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00507. 2 interactions.
STRINGi10116.ENSRNOP00000015956.

Chemistry

ChEMBLiCHEMBL2351.

PTM databases

iPTMnetiP00507.
PhosphoSiteiP00507.

Proteomic databases

PaxDbiP00507.
PRIDEiP00507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782.
GeneIDi25721.
KEGGirno:25721.
UCSCiRGD:2722. rat.

Organism-specific databases

CTDi2806.
RGDi2722. Got2.

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00507.
KOiK14455.
OMAiWANHAAV.
OrthoDBiEOG091G06G3.
PhylomeDBiP00507.
TreeFamiTF300641.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15069.
BRENDAi2.6.1.1. 5301.
ReactomeiR-RNO-70263. Gluconeogenesis.
R-RNO-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKP00507.

Miscellaneous databases

PROiP00507.

Gene expression databases

BgeeiENSRNOG00000011782.
GenevisibleiP00507. RN.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAATM_RAT
AccessioniPrimary (citable) accession number: P00507
Secondary accession number(s): Q64551, Q9QV50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: September 7, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.