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Protein

Aspartate aminotransferase, mitochondrial

Gene

Got2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity1 Publication

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.1 Publication
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

  • amino acid binding Source: RGD
  • carboxylic acid binding Source: RGD
  • enzyme binding Source: RGD
  • kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
  • L-aspartate:2-oxoglutarate aminotransferase activity Source: RGD
  • L-cysteine:2-oxoglutarate aminotransferase activity Source: Reactome
  • phospholipid binding Source: RGD
  • protein homodimerization activity Source: RGD
  • pyridoxal phosphate binding Source: RGD

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: UniProtKB
  • aspartate biosynthetic process Source: Ensembl
  • aspartate catabolic process Source: GO_Central
  • aspartate metabolic process Source: RGD
  • cellular amino acid metabolic process Source: RGD
  • dicarboxylic acid metabolic process Source: RGD
  • fatty acid transport Source: RGD
  • female pregnancy Source: RGD
  • glutamate catabolic process to 2-oxoglutarate Source: RGD
  • glutamate catabolic process to aspartate Source: Ensembl
  • glutamate metabolic process Source: UniProtKB
  • lactation Source: RGD
  • oxaloacetate metabolic process Source: RGD
  • response to ethanol Source: Ensembl
  • response to insulin Source: RGD
  • response to morphine Source: RGD
  • response to muscle activity Source: RGD

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processLipid transport, Transport
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15069
BRENDAi2.6.1.1 5301
SABIO-RKiP00507

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:Got2
Synonyms:Maat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi2722 Got2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2351

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Mitochondrion4 PublicationsAdd BLAST29
ChainiPRO_000000121830 – 430Aspartate aminotransferase, mitochondrialAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphothreonineBy similarity1
Modified residuei59N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateBy similarity1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei90N6-acetyllysine; alternateBy similarity1
Modified residuei90N6-succinyllysine; alternateBy similarity1
Modified residuei96Nitrated tyrosine; alternateBy similarity1
Modified residuei96Phosphotyrosine; alternateBy similarity1
Modified residuei107N6-acetyllysine; alternateBy similarity1
Modified residuei107N6-succinyllysine; alternateBy similarity1
Modified residuei122N6-acetyllysine; alternateBy similarity1
Modified residuei122N6-succinyllysine; alternateBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei159N6-acetyllysine; alternateBy similarity1
Modified residuei159N6-succinyllysine; alternateBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei227N6-succinyllysineBy similarity1
Modified residuei234N6-acetyllysineBy similarity1
Modified residuei279N6-(pyridoxal phosphate)lysine; alternate1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei309N6-acetyllysine; alternateBy similarity1
Modified residuei309N6-succinyllysine; alternateBy similarity1
Modified residuei313Asymmetric dimethylarginineBy similarity1
Modified residuei338N6-acetyllysine; alternateBy similarity1
Modified residuei338N6-succinyllysine; alternateBy similarity1
Modified residuei345N6-acetyllysineBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei387N6-acetyllysineBy similarity1
Modified residuei396N6-acetyllysine; alternateBy similarity1
Modified residuei396N6-succinyllysine; alternateBy similarity1
Modified residuei404N6-acetyllysine; alternateBy similarity1
Modified residuei404N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP00507
PRIDEiP00507

PTM databases

CarbonylDBiP00507
iPTMnetiP00507
PhosphoSitePlusiP00507

Expressioni

Tissue specificityi

Expressed in all tissues tested: liver, pancreas, kidney, heart, spleen, arterioles, and lymphocytes.1 Publication

Gene expression databases

BgeeiENSRNOG00000011782
GenevisibleiP00507 RN

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP00507, 2 interactors
STRINGi10116.ENSRNOP00000015956

Structurei

3D structure databases

ProteinModelPortaliP00507
SMRiP00507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP00507
KOiK14455
OMAiRVGACTI
OrthoDBiEOG091G06G3
PhylomeDBiP00507
TreeFamiTF300641

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLHSGRVL SGMAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAGK NLDKEYLPIG
110 120 130 140 150
GLADFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRV GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FSGALEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEMA AVVKKKNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPLYSNPP LNGARIAATI LTSPDLRKQW LQEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHITDQIGM FCFTGLKPEQ VERLTKEFSV
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Length:430
Mass (Da):47,314
Last modified:July 1, 1989 - v2
Checksum:iEDC8B862A20DB736
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30S → R in AAC13868 (PubMed:7759512).Curated1
Sequence conflicti162W → G AA sequence (PubMed:7470110).Curated1
Sequence conflicti162W → G AA sequence (PubMed:7309704).Curated1
Sequence conflicti167 – 169PIF → EIA AA sequence (PubMed:7470110).Curated3
Sequence conflicti167 – 169PIF → EIA AA sequence (PubMed:7309704).Curated3
Sequence conflicti177Q → E AA sequence (PubMed:7470110).Curated1
Sequence conflicti177Q → E AA sequence (PubMed:7309704).Curated1
Sequence conflicti232V → Y AA sequence (PubMed:7470110).Curated1
Sequence conflicti232V → Y AA sequence (PubMed:7309704).Curated1
Sequence conflicti255D → N AA sequence (PubMed:7470110).Curated1
Sequence conflicti255D → N AA sequence (PubMed:7309704).Curated1
Sequence conflicti338 – 339KQ → QG AA sequence (PubMed:7470110).Curated2
Sequence conflicti338 – 339KQ → QG AA sequence (PubMed:7309704).Curated2
Sequence conflicti352I → G AA sequence (PubMed:7470110).Curated1
Sequence conflicti352I → G AA sequence (PubMed:7309704).Curated1
Sequence conflicti386L → I AA sequence (PubMed:7470110).Curated1
Sequence conflicti386L → I AA sequence (PubMed:7309704).Curated1
Sequence conflicti400V → I AA sequence (PubMed:7470110).Curated1
Sequence conflicti400V → I AA sequence (PubMed:7309704).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18467 mRNA Translation: AAB54275.1
BC061792 mRNA Translation: AAH61792.1
U21158 Genomic DNA Translation: AAC13868.1
M12709 mRNA Translation: AAA41267.1
PIRiA28005 XNRTDM
I55427
RefSeqiNP_037309.1, NM_013177.2
UniGeneiRn.98650

Genome annotation databases

EnsembliENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782
GeneIDi25721
KEGGirno:25721
UCSCiRGD:2722 rat

Similar proteinsi

Entry informationi

Entry nameiAATM_RAT
AccessioniPrimary (citable) accession number: P00507
Secondary accession number(s): Q64551, Q9QV50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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