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Protein

Aspartate aminotransferase, mitochondrial

Gene

GOT2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processLipid transport, Transport
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:GOT2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Mitochondrion2 PublicationsAdd BLAST29
ChainiPRO_000000121730 – 430Aspartate aminotransferase, mitochondrialAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphothreonineBy similarity1
Modified residuei59N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateBy similarity1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei90N6-acetyllysine; alternateBy similarity1
Modified residuei90N6-succinyllysine; alternateBy similarity1
Modified residuei96Nitrated tyrosine; alternateBy similarity1
Modified residuei96Phosphotyrosine; alternateBy similarity1
Modified residuei107N6-acetyllysine; alternateBy similarity1
Modified residuei107N6-succinyllysine; alternateBy similarity1
Modified residuei122N6-acetyllysine; alternateBy similarity1
Modified residuei122N6-succinyllysine; alternateBy similarity1
Modified residuei159N6-acetyllysine; alternateBy similarity1
Modified residuei159N6-succinyllysine; alternateBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei227N6-succinyllysineBy similarity1
Modified residuei234N6-acetyllysineBy similarity1
Modified residuei279N6-(pyridoxal phosphate)lysine; alternate1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei309N6-acetyllysine; alternateBy similarity1
Modified residuei309N6-succinyllysine; alternateBy similarity1
Modified residuei313Asymmetric dimethylarginineBy similarity1
Modified residuei345N6-acetyllysineBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei387N6-acetyllysineBy similarity1
Modified residuei396N6-acetyllysine; alternateBy similarity1
Modified residuei396N6-succinyllysine; alternateBy similarity1
Modified residuei404N6-acetyllysine; alternateBy similarity1
Modified residuei404N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP00506
PeptideAtlasiP00506
PRIDEiP00506

PTM databases

iPTMnetiP00506

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000003017

Structurei

3D structure databases

ProteinModelPortaliP00506
SMRiP00506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP00506
KOiK14455

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLHSGRVL SGVASAFHPG LAAAASARAS SWWAHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIG
110 120 130 140 150
GLAEFCKASA ELALGENNEV LKSGRYVTVQ TISGTGALRI GANFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLHSYR YYDPKTCGFD FTGALEDISK
210 220 230 240 250
IPAQSVILLH ACAHNPTGVD PRPEQWKEMA TLVKKNNLFA FFDMAYQGFA
260 270 280 290 300
SGDGNKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP VNGARIASTI LTSPDLRQQW LQEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHIVDQIGM FCFTGIKPEQ VERLTKEFSI
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Length:430
Mass (Da):47,436
Last modified:October 1, 1989 - v2
Checksum:i23280D11821A8BC4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71N → D AA sequence (PubMed:7391012).Curated1
Sequence conflicti256K → R in CAA23279 (PubMed:8672129).Curated1
Sequence conflicti273L → F in CAA23279 (PubMed:8672129).Curated1
Sequence conflicti305E → Q AA sequence (PubMed:7408859).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11732 mRNA Translation: AAA30999.1
F14822 mRNA Translation: CAA23279.1
PIRiA25165 XNPGDM
RefSeqiNP_999093.1, NM_213928.1
UniGeneiSsc.3588

Genome annotation databases

EnsembliENSSSCT00000041306; ENSSSCP00000037719; ENSSSCG00000032985
GeneIDi396968
KEGGissc:396968

Similar proteinsi

Entry informationi

Entry nameiAATM_PIG
AccessioniPrimary (citable) accession number: P00506
Secondary accession number(s): Q29230
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 1, 1989
Last modified: April 25, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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