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Protein

Aspartate aminotransferase, mitochondrial

Gene

GOT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.1 Publication

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS04858-MONOMER.
ZFISH:HS04858-MONOMER.
ReactomeiR-HSA-70263. Gluconeogenesis.
R-HSA-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:GOT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:4433. GOT2.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: Ensembl
  • extracellular exosome Source: UniProtKB
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial matrix Source: Reactome
  • mitochondrion Source: UniProtKB
  • myelin sheath Source: Ensembl
  • perikaryon Source: Ensembl
  • plasma membrane Source: UniProtKB
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi2806.
OpenTargetsiENSG00000125166.
PharmGKBiPA28818.

Chemistry databases

DrugBankiDB00128. L-Aspartic Acid.

Polymorphism and mutation databases

BioMutaiGOT2.
DMDMi308153643.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionCombined sources1 PublicationAdd BLAST29
ChainiPRO_000000121530 – 430Aspartate aminotransferase, mitochondrialAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphothreonineCombined sources1
Modified residuei59N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateCombined sources1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei90N6-acetyllysine; alternateCombined sources1
Modified residuei90N6-succinyllysine; alternateBy similarity1
Modified residuei96Nitrated tyrosine; alternateBy similarity1
Modified residuei96Phosphotyrosine; alternateCombined sources1
Modified residuei107N6-acetyllysine; alternateBy similarity1
Modified residuei107N6-succinyllysine; alternateBy similarity1
Modified residuei122N6-acetyllysine; alternateBy similarity1
Modified residuei122N6-succinyllysine; alternateBy similarity1
Modified residuei143PhosphoserineCombined sources1
Modified residuei159N6-acetyllysine; alternateCombined sources1
Modified residuei159N6-succinyllysine; alternateBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei227N6-succinyllysineBy similarity1
Modified residuei234N6-acetyllysineCombined sources1
Modified residuei279N6-(pyridoxal phosphate)lysine; alternateBy similarity1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-acetyllysine; alternateCombined sources1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei309N6-acetyllysine; alternateBy similarity1
Modified residuei309N6-succinyllysine; alternateBy similarity1
Modified residuei313Asymmetric dimethylarginineBy similarity1
Modified residuei333PhosphothreonineCombined sources1
Modified residuei338N6-acetyllysine; alternateBy similarity1
Modified residuei338N6-succinyllysine; alternateBy similarity1
Modified residuei345N6-acetyllysineBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei387N6-acetyllysineBy similarity1
Modified residuei396N6-acetyllysine; alternateCombined sources1
Modified residuei396N6-succinyllysine; alternateBy similarity1
Modified residuei404N6-acetyllysine; alternateCombined sources1
Modified residuei404N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

EPDiP00505.
MaxQBiP00505.
PaxDbiP00505.
PeptideAtlasiP00505.
PRIDEiP00505.
TopDownProteomicsiP00505-1. [P00505-1]

2D gel databases

UCD-2DPAGEP00505.

PTM databases

iPTMnetiP00505.
PhosphoSitePlusiP00505.
SwissPalmiP00505.

Expressioni

Inductioni

Up-regulated by long-time exposure to alcohol.1 Publication

Gene expression databases

BgeeiENSG00000125166.
CleanExiHS_GOT2.
ExpressionAtlasiP00505. baseline and differential.
GenevisibleiP00505. HS.

Organism-specific databases

HPAiHPA018139.

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109068. 21 interactors.
IntActiP00505. 8 interactors.
MINTiMINT-1406848.
STRINGi9606.ENSP00000245206.

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi43 – 53Combined sources11
Helixi78 – 88Combined sources11
Turni89 – 91Combined sources3
Helixi103 – 114Combined sources12
Helixi119 – 123Combined sources5
Beta strandi126 – 132Combined sources7
Helixi133 – 148Combined sources16
Beta strandi153 – 159Combined sources7
Helixi165 – 172Combined sources8
Beta strandi175 – 180Combined sources6
Turni184 – 187Combined sources4
Helixi191 – 198Combined sources8
Beta strandi206 – 213Combined sources8
Helixi223 – 236Combined sources14
Beta strandi239 – 246Combined sources8
Turni247 – 251Combined sources5
Helixi254 – 257Combined sources4
Helixi259 – 266Combined sources8
Beta strandi272 – 276Combined sources5
Turni278 – 280Combined sources3
Turni284 – 287Combined sources4
Beta strandi288 – 294Combined sources7
Helixi298 – 315Combined sources18
Beta strandi316 – 318Combined sources3
Helixi321 – 332Combined sources12
Helixi334 – 364Combined sources31
Helixi372 – 376Combined sources5
Beta strandi379 – 383Combined sources5
Helixi388 – 398Combined sources11
Beta strandi406 – 409Combined sources4
Helixi410 – 412Combined sources3
Helixi415 – 428Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AX8X-ray2.99A/B/C/D30-430[»]
ProteinModelPortaliP00505.
SMRiP00505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00505.
KOiK14455.
OMAiWANHAAV.
OrthoDBiEOG091G06G3.
PhylomeDBiP00505.
TreeFamiTF300641.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P00505-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLHSGRVL PGIAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIG
110 120 130 140 150
GLAEFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRI GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP TWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FTGAVEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTMVCKDADE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP LNGARIAAAI LNTPDLRKQW LQEVKVMADR
360 370 380 390 400
IIGMRTQLVS NLKKEGSTHN WQHITDQIGM FCFTGLKPEQ VERLIKEFSI
410 420 430
YMTKDGRISV AGVTSSNVGY LAHAIHQVTK
Length:430
Mass (Da):47,518
Last modified:October 5, 2010 - v3
Checksum:iF559567ABF2DB346
GO
Isoform 2 (identifier: P00505-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-125: Missing.

Show »
Length:387
Mass (Da):43,030
Checksum:iFF09030A2B5C4E62
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 111AE → EA AA sequence (PubMed:4052435).Curated2
Sequence conflicti255D → N AA sequence (PubMed:4052435).Curated1
Sequence conflicti258A → T in BAD96991 (Ref. 3) Curated1
Sequence conflicti305E → Q AA sequence (PubMed:4052435).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0554942A → S.Corresponds to variant rs11558171dbSNPEnsembl.1
Natural variantiVAR_031710188G → S.Corresponds to variant rs11076256dbSNPEnsembl.1
Natural variantiVAR_031711346V → G.3 PublicationsCorresponds to variant rs30842dbSNPEnsembl.1
Natural variantiVAR_031712428V → A.1 PublicationCorresponds to variant rs17849335dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05484883 – 125Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22632 mRNA. Translation: AAA35568.1.
AK295993 mRNA. Translation: BAG58768.1.
AK223271 mRNA. Translation: BAD96991.1.
AC012183 Genomic DNA. No translation available.
BC000525 mRNA. Translation: AAH00525.1.
CCDSiCCDS10801.1. [P00505-1]
CCDS67045.1. [P00505-2]
PIRiA31873. XNHUDM.
RefSeqiNP_001273149.1. NM_001286220.1. [P00505-2]
NP_002071.2. NM_002080.3. [P00505-1]
UniGeneiHs.599470.

Genome annotation databases

EnsembliENST00000245206; ENSP00000245206; ENSG00000125166. [P00505-1]
ENST00000434819; ENSP00000394100; ENSG00000125166. [P00505-2]
GeneIDi2806.
KEGGihsa:2806.
UCSCiuc002eof.2. human. [P00505-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22632 mRNA. Translation: AAA35568.1.
AK295993 mRNA. Translation: BAG58768.1.
AK223271 mRNA. Translation: BAD96991.1.
AC012183 Genomic DNA. No translation available.
BC000525 mRNA. Translation: AAH00525.1.
CCDSiCCDS10801.1. [P00505-1]
CCDS67045.1. [P00505-2]
PIRiA31873. XNHUDM.
RefSeqiNP_001273149.1. NM_001286220.1. [P00505-2]
NP_002071.2. NM_002080.3. [P00505-1]
UniGeneiHs.599470.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AX8X-ray2.99A/B/C/D30-430[»]
ProteinModelPortaliP00505.
SMRiP00505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109068. 21 interactors.
IntActiP00505. 8 interactors.
MINTiMINT-1406848.
STRINGi9606.ENSP00000245206.

Chemistry databases

DrugBankiDB00128. L-Aspartic Acid.

PTM databases

iPTMnetiP00505.
PhosphoSitePlusiP00505.
SwissPalmiP00505.

Polymorphism and mutation databases

BioMutaiGOT2.
DMDMi308153643.

2D gel databases

UCD-2DPAGEP00505.

Proteomic databases

EPDiP00505.
MaxQBiP00505.
PaxDbiP00505.
PeptideAtlasiP00505.
PRIDEiP00505.
TopDownProteomicsiP00505-1. [P00505-1]

Protocols and materials databases

DNASUi2806.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000245206; ENSP00000245206; ENSG00000125166. [P00505-1]
ENST00000434819; ENSP00000394100; ENSG00000125166. [P00505-2]
GeneIDi2806.
KEGGihsa:2806.
UCSCiuc002eof.2. human. [P00505-1]

Organism-specific databases

CTDi2806.
DisGeNETi2806.
GeneCardsiGOT2.
H-InvDBHIX0013095.
HGNCiHGNC:4433. GOT2.
HPAiHPA018139.
MIMi138150. gene.
neXtProtiNX_P00505.
OpenTargetsiENSG00000125166.
PharmGKBiPA28818.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00505.
KOiK14455.
OMAiWANHAAV.
OrthoDBiEOG091G06G3.
PhylomeDBiP00505.
TreeFamiTF300641.

Enzyme and pathway databases

BioCyciMetaCyc:HS04858-MONOMER.
ZFISH:HS04858-MONOMER.
ReactomeiR-HSA-70263. Gluconeogenesis.
R-HSA-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

ChiTaRSiGOT2. human.
GeneWikiiGOT2.
GenomeRNAii2806.
PROiP00505.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125166.
CleanExiHS_GOT2.
ExpressionAtlasiP00505. baseline and differential.
GenevisibleiP00505. HS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAATM_HUMAN
AccessioniPrimary (citable) accession number: P00505
Secondary accession number(s): B4DJA6
, E7ERW2, Q53FL3, Q9BWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 182 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.