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Protein

Aspartate aminotransferase, cytoplasmic

Gene

GOT1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Aspartate; via amide nitrogen1
Binding sitei140Aspartate1
Binding sitei194Aspartate1
Binding sitei386Aspartate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-GGA-352875. Gluconeogenesis.
R-GGA-372568. Amino acid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1, EC:2.6.1.3)
Short name:
cAspAT
Alternative name(s):
Cysteine aminotransferase, cytoplasmic
Cysteine transaminase, cytoplasmic
Short name:
cCAT
Glutamate oxaloacetate transaminase 1
Transaminase A
Gene namesi
Name:GOT1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001238842 – 412Aspartate aminotransferase, cytoplasmicAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei258N6-(pyridoxal phosphate)lysine1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00504.
PRIDEiP00504.

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012086.

Structurei

Secondary structure

1412
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 8Combined sources4
Helixi16 – 26Combined sources11
Helixi51 – 62Combined sources12
Helixi77 – 88Combined sources12
Helixi93 – 96Combined sources4
Beta strandi100 – 106Combined sources7
Helixi107 – 122Combined sources16
Beta strandi123 – 127Combined sources5
Beta strandi133 – 138Combined sources6
Helixi142 – 149Combined sources8
Beta strandi155 – 158Combined sources4
Turni163 – 166Combined sources4
Helixi170 – 178Combined sources9
Beta strandi185 – 192Combined sources8
Turni194 – 196Combined sources3
Helixi202 – 215Combined sources14
Beta strandi218 – 224Combined sources7
Turni226 – 230Combined sources5
Helixi233 – 236Combined sources4
Helixi238 – 245Combined sources8
Beta strandi250 – 255Combined sources6
Turni257 – 259Combined sources3
Helixi263 – 265Combined sources3
Beta strandi267 – 273Combined sources7
Helixi277 – 292Combined sources16
Turni293 – 295Combined sources3
Helixi301 – 311Combined sources11
Helixi313 – 344Combined sources32
Helixi352 – 355Combined sources4
Beta strandi358 – 362Combined sources5
Helixi367 – 375Combined sources9
Beta strandi386 – 388Combined sources3
Helixi389 – 391Combined sources3
Turni394 – 396Combined sources3
Helixi397 – 410Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AATX-ray2.80A/B2-412[»]
2CSTX-ray1.90A/B2-412[»]
ProteinModelPortaliP00504.
SMRiP00504.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00504.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00504.
KOiK14454.
PhylomeDBiP00504.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASIFAAVP RAPPVAVFKL TADFREDGDS RKVNLGVGAY RTDEGQPWVL
60 70 80 90 100
PVVRKVEQLI AGDGSLNHEY LPILGLPEFR ANASRIALGD DSPAIAQKRV
110 120 130 140 150
GSVQGLGGTG ALRIGAEFLR RWYNGNNNTA TPVYVSSPTW ENHNSVFMDA
160 170 180 190 200
GFKDIRTYRY WDAAKRGLDL QGLLDDMEKA PEFSIFILHA CAHNPTGTDP
210 220 230 240 250
TPDEWKQIAA VMKRRCLFPF FDSAYQGFAS GSLDKDAWAV RYFVSEGFEL
260 270 280 290 300
FCAQSFSKNF GLYNERVGNL SVVGKDEDNV QRVLSQMEKI VRTTWSNPPS
310 320 330 340 350
QGARIVATTL TSPQLFAEWK DNVKTMADRV LLMRSELRSR LESLGTPGTW
360 370 380 390 400
NHITDQIGMF SFTGLNPKQV EYMIKEKHIY LMASGRINMC GLTTKNLDYV
410
AKSIHEAVTK IQ
Length:412
Mass (Da):45,935
Last modified:January 23, 2007 - v3
Checksum:iC55BEE72669078E1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63D → N AA sequence (PubMed:499525).Curated1
Sequence conflicti63D → N AA sequence (Ref. 3) Curated1
Sequence conflicti121Missing AA sequence (PubMed:499525).Curated1
Sequence conflicti121Missing AA sequence (Ref. 3) Curated1
Sequence conflicti140W → S AA sequence (Ref. 3) Curated1
Sequence conflicti175D → S AA sequence (PubMed:499525).Curated1
Sequence conflicti175D → S AA sequence (Ref. 3) Curated1
Sequence conflicti232 – 234SLD → NLE AA sequence (PubMed:499525).Curated3
Sequence conflicti232 – 234SLD → NLE AA sequence (Ref. 3) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15636 mRNA. Translation: CAA33646.1.
PIRiS05583. XNCHDC.
RefSeqiNP_990652.1. NM_205321.1.
UniGeneiGga.730.

Genome annotation databases

GeneIDi396261.
KEGGigga:396261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15636 mRNA. Translation: CAA33646.1.
PIRiS05583. XNCHDC.
RefSeqiNP_990652.1. NM_205321.1.
UniGeneiGga.730.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AATX-ray2.80A/B2-412[»]
2CSTX-ray1.90A/B2-412[»]
ProteinModelPortaliP00504.
SMRiP00504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012086.

Proteomic databases

PaxDbiP00504.
PRIDEiP00504.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396261.
KEGGigga:396261.

Organism-specific databases

CTDi2805.

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00504.
KOiK14454.
PhylomeDBiP00504.

Enzyme and pathway databases

ReactomeiR-GGA-352875. Gluconeogenesis.
R-GGA-372568. Amino acid metabolism.

Miscellaneous databases

EvolutionaryTraceiP00504.
PROiP00504.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAATC_CHICK
AccessioniPrimary (citable) accession number: P00504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.