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Protein

Aspartate aminotransferase, cytoplasmic

Gene

GOT1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity).By similarity

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.1 Publication
L-cysteine + 2-oxoglutarate = mercaptopyruvate + L-glutamate.1 Publication

Cofactori

pyridoxal 5'-phosphate2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Aspartate; via amide nitrogen1
Binding sitei141Aspartate1
Binding sitei195Aspartate1
Binding sitei387Aspartate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13031.
ReactomeiR-SSC-70263. Gluconeogenesis.
R-SSC-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1, EC:2.6.1.3)
Short name:
cAspAT
Alternative name(s):
Cysteine aminotransferase, cytoplasmic
Cysteine transaminase, cytoplasmic
Short name:
cCAT
Glutamate oxaloacetate transaminase 1
Transaminase A
Gene namesi
Name:GOT1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001238812 – 413Aspartate aminotransferase, cytoplasmicAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei259N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP00503.
PeptideAtlasiP00503.
PRIDEiP00503.

Expressioni

Gene expression databases

BgeeiENSSSCG00000010537.
GenevisibleiP00503. SS.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011226.

Structurei

Secondary structure

1413
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 9Combined sources4
Helixi17 – 26Combined sources10
Helixi52 – 62Combined sources11
Helixi78 – 89Combined sources12
Helixi94 – 97Combined sources4
Beta strandi101 – 107Combined sources7
Helixi108 – 123Combined sources16
Beta strandi124 – 128Combined sources5
Beta strandi134 – 139Combined sources6
Helixi143 – 150Combined sources8
Beta strandi156 – 160Combined sources5
Turni164 – 167Combined sources4
Helixi171 – 180Combined sources10
Beta strandi186 – 193Combined sources8
Turni195 – 197Combined sources3
Helixi203 – 216Combined sources14
Beta strandi219 – 225Combined sources7
Turni227 – 231Combined sources5
Helixi234 – 237Combined sources4
Helixi239 – 246Combined sources8
Beta strandi251 – 256Combined sources6
Turni258 – 260Combined sources3
Helixi264 – 266Combined sources3
Beta strandi268 – 274Combined sources7
Helixi278 – 293Combined sources16
Turni294 – 296Combined sources3
Helixi302 – 312Combined sources11
Helixi314 – 344Combined sources31
Helixi352 – 356Combined sources5
Beta strandi359 – 363Combined sources5
Helixi368 – 376Combined sources9
Beta strandi386 – 389Combined sources4
Helixi390 – 392Combined sources3
Turni395 – 397Combined sources3
Helixi398 – 411Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJRX-ray1.74A/B2-413[»]
1AJSX-ray1.60A/B2-413[»]
ProteinModelPortaliP00503.
SMRiP00503.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00503.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00503.
KOiK14454.
OMAiPNHKGVF.
OrthoDBiEOG091G06G3.
TreeFamiTF314089.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPSVFAEV PQAQPVLVFK LIADFREDPD PRKVNLGVGA YRTDDCQPWV
60 70 80 90 100
LPVVRKVEQR IANDSSLNHE YLPILGLAEF RTCASRLALG DDSPALQEKR
110 120 130 140 150
VGGVQSLGGT GALRIGAEFL ARWYNGTNNK DTPVYVSSPT WENHNGVFTT
160 170 180 190 200
AGFKDIRSYR YWDTEKRGLD LQGFLSDLEN APEFSIFVLH ACAHNPTGTD
210 220 230 240 250
PTPEQWKQIA SVMKRRFLFP FFDSAYQGFA SGNLEKDAWA IRYFVSEGFE
260 270 280 290 300
LFCAQSFSKN FGLYNERVGN LTVVAKEPDS ILRVLSQMEK IVRVTWSNPP
310 320 330 340 350
AQGARIVART LSDPELFHEW TGNVKTMADR ILSMRSELRA RLEALKTPGT
360 370 380 390 400
WNHITDQIGM FSFTGLNPKQ VEYLINEKHI YLLPSGRINM CGLTTKNLDY
410
VATSIHEAVT KIQ
Length:413
Mass (Da):46,475
Last modified:January 23, 2007 - v3
Checksum:iE466EDAD9446EF25
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145N → D AA sequence (PubMed:11946901).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24088 mRNA. Translation: AAA53531.1.
PIRiA30138. XNPGDC.
RefSeqiNP_999092.1. NM_213927.1.
UniGeneiSsc.3528.

Genome annotation databases

EnsembliENSSSCT00000011527; ENSSSCP00000011226; ENSSSCG00000010537.
GeneIDi396967.
KEGGissc:396967.

Cross-referencesi

Web resourcesi

Worthington enzyme manual

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24088 mRNA. Translation: AAA53531.1.
PIRiA30138. XNPGDC.
RefSeqiNP_999092.1. NM_213927.1.
UniGeneiSsc.3528.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJRX-ray1.74A/B2-413[»]
1AJSX-ray1.60A/B2-413[»]
ProteinModelPortaliP00503.
SMRiP00503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011226.

Proteomic databases

PaxDbiP00503.
PeptideAtlasiP00503.
PRIDEiP00503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000011527; ENSSSCP00000011226; ENSSSCG00000010537.
GeneIDi396967.
KEGGissc:396967.

Organism-specific databases

CTDi2805.

Phylogenomic databases

eggNOGiKOG1411. Eukaryota.
COG1448. LUCA.
GeneTreeiENSGT00390000014081.
HOGENOMiHOG000185898.
HOVERGENiHBG000951.
InParanoidiP00503.
KOiK14454.
OMAiPNHKGVF.
OrthoDBiEOG091G06G3.
TreeFamiTF314089.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13031.
ReactomeiR-SSC-70263. Gluconeogenesis.
R-SSC-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

EvolutionaryTraceiP00503.

Gene expression databases

BgeeiENSSSCG00000010537.
GenevisibleiP00503. SS.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR000796. Asp_trans.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11879. PTHR11879. 1 hit.
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
PRINTSiPR00799. TRANSAMINASE.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAATC_PIG
AccessioniPrimary (citable) accession number: P00503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.