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P00493

- HPRT_MOUSE

UniProt

P00493 - HPRT_MOUSE

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691GMPBy similarity
    Active sitei138 – 1381Proton acceptorBy similarity
    Binding sitei166 – 1661GMPBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 1429GMPBy similarity
    Nucleotide bindingi186 – 1883GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB
    2. hypoxanthine phosphoribosyltransferase activity Source: MGI
    3. identical protein binding Source: MGI
    4. magnesium ion binding Source: RefGenome
    5. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. adenine metabolic process Source: MGI
    2. adenine salvage Source: MGI
    3. central nervous system neuron development Source: MGI
    4. cerebral cortex neuron differentiation Source: MGI
    5. cytolysis Source: MGI
    6. dendrite morphogenesis Source: MGI
    7. dopamine metabolic process Source: MGI
    8. GMP catabolic process Source: UniProtKB
    9. GMP salvage Source: RefGenome
    10. grooming behavior Source: MGI
    11. guanine salvage Source: MGI
    12. hypoxanthine metabolic process Source: MGI
    13. hypoxanthine salvage Source: UniProtKB
    14. IMP metabolic process Source: UniProtKB
    15. IMP salvage Source: RefGenome
    16. locomotory behavior Source: MGI
    17. lymphocyte proliferation Source: MGI
    18. positive regulation of dopamine metabolic process Source: MGI
    19. protein homotetramerization Source: Ensembl
    20. purine ribonucleoside salvage Source: MGI
    21. response to amphetamine Source: MGI
    22. striatum development Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Alternative name(s):
    HPRT B
    Gene namesi
    Name:Hprt1
    Synonyms:Hprt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:96217. Hprt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytosol Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139588Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei103 – 1031N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP00493.
    PaxDbiP00493.
    PRIDEiP00493.

    PTM databases

    PhosphoSiteiP00493.

    Expressioni

    Gene expression databases

    BgeeiP00493.
    CleanExiMM_HPRT1.
    GenevestigatoriP00493.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    DIPiDIP-6034N.
    MINTiMINT-217744.
    STRINGi10090.ENSMUSP00000026723.

    Structurei

    3D structure databases

    ProteinModelPortaliP00493.
    SMRiP00493. Positions 5-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    GeneTreeiENSGT00390000017323.
    HOGENOMiHOG000236521.
    HOVERGENiHBG000242.
    InParanoidiP00493.
    KOiK00760.
    OMAiAREIMKG.
    OrthoDBiEOG7673CK.
    PhylomeDBiP00493.
    TreeFamiTF313367.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00493-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPTRSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA    50
    RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI 100
    RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV 150
    KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEYFR 200
    DLNHVCVISE TGKAKYKA 218
    Length:218
    Mass (Da):24,570
    Last modified:January 23, 2007 - v3
    Checksum:i925CC0D4A6626E05
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti201 – 2011D → N in AAA96232. (PubMed:6294614)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00423 mRNA. Translation: AAA96232.1.
    K01515
    , K01507, K01508, K01509, K01510, K01511, K01512, K01513, K01514 Genomic DNA. Translation: AAA96271.1.
    AK002286 mRNA. Translation: BAB21989.1.
    AK088114 mRNA. Translation: BAC40153.1.
    AK146626 mRNA. Translation: BAE27315.1.
    BC083145 mRNA. Translation: AAH83145.1.
    CCDSiCCDS40972.1.
    PIRiI49756. RTMSG.
    RefSeqiNP_038584.2. NM_013556.2.
    UniGeneiMm.299381.

    Genome annotation databases

    EnsembliENSMUST00000026723; ENSMUSP00000026723; ENSMUSG00000025630.
    GeneIDi15452.
    KEGGimmu:15452.
    UCSCiuc009ter.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J00423 mRNA. Translation: AAA96232.1 .
    K01515
    , K01507 , K01508 , K01509 , K01510 , K01511 , K01512 , K01513 , K01514 Genomic DNA. Translation: AAA96271.1 .
    AK002286 mRNA. Translation: BAB21989.1 .
    AK088114 mRNA. Translation: BAC40153.1 .
    AK146626 mRNA. Translation: BAE27315.1 .
    BC083145 mRNA. Translation: AAH83145.1 .
    CCDSi CCDS40972.1.
    PIRi I49756. RTMSG.
    RefSeqi NP_038584.2. NM_013556.2.
    UniGenei Mm.299381.

    3D structure databases

    ProteinModelPortali P00493.
    SMRi P00493. Positions 5-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6034N.
    MINTi MINT-217744.
    STRINGi 10090.ENSMUSP00000026723.

    PTM databases

    PhosphoSitei P00493.

    Proteomic databases

    MaxQBi P00493.
    PaxDbi P00493.
    PRIDEi P00493.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026723 ; ENSMUSP00000026723 ; ENSMUSG00000025630 .
    GeneIDi 15452.
    KEGGi mmu:15452.
    UCSCi uc009ter.1. mouse.

    Organism-specific databases

    CTDi 15452.
    MGIi MGI:96217. Hprt.

    Phylogenomic databases

    eggNOGi COG0634.
    GeneTreei ENSGT00390000017323.
    HOGENOMi HOG000236521.
    HOVERGENi HBG000242.
    InParanoidi P00493.
    KOi K00760.
    OMAi AREIMKG.
    OrthoDBi EOG7673CK.
    PhylomeDBi P00493.
    TreeFami TF313367.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .

    Miscellaneous databases

    ChiTaRSi HPRT1. mouse.
    NextBioi 288260.
    PROi P00493.
    SOURCEi Search...

    Gene expression databases

    Bgeei P00493.
    CleanExi MM_HPRT1.
    Genevestigatori P00493.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants."
      Konecki D.S., Brennand J., Fuscoe J.C., Caskey C.T., Chinault A.C.
      Nucleic Acids Res. 10:6763-6775(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene."
      Melton D.W., Konecki D.S., Brennand J., Caskey C.T.
      Proc. Natl. Acad. Sci. U.S.A. 81:2147-2151(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Myeloma.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Heart and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    5. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 74-83 AND 171-200, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-103, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiHPRT_MOUSE
    AccessioniPrimary (citable) accession number: P00493
    Secondary accession number(s): Q545Y2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3