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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691GMPBy similarity
Active sitei138 – 1381Proton acceptorBy similarity
Binding sitei166 – 1661GMPBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429GMPBy similarity
Nucleotide bindingi186 – 1883GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB
  2. hypoxanthine phosphoribosyltransferase activity Source: MGI
  3. identical protein binding Source: MGI
  4. magnesium ion binding Source: MGI
  5. nucleotide binding Source: UniProtKB-KW
  6. protein homodimerization activity Source: MGI

GO - Biological processi

  1. adenine metabolic process Source: MGI
  2. adenine salvage Source: MGI
  3. central nervous system neuron development Source: MGI
  4. cerebral cortex neuron differentiation Source: MGI
  5. cytolysis Source: MGI
  6. dendrite morphogenesis Source: MGI
  7. dopamine metabolic process Source: MGI
  8. GMP catabolic process Source: UniProtKB
  9. GMP salvage Source: GO_Central
  10. grooming behavior Source: MGI
  11. guanine salvage Source: MGI
  12. hypoxanthine metabolic process Source: MGI
  13. hypoxanthine salvage Source: UniProtKB
  14. IMP metabolic process Source: UniProtKB
  15. IMP salvage Source: GO_Central
  16. locomotory behavior Source: MGI
  17. lymphocyte proliferation Source: MGI
  18. positive regulation of dopamine metabolic process Source: MGI
  19. protein homotetramerization Source: MGI
  20. purine nucleotide biosynthetic process Source: MGI
  21. purine ribonucleoside salvage Source: MGI
  22. response to amphetamine Source: MGI
  23. striatum development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_345398. Purine salvage.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Alternative name(s):
HPRT B
Gene namesi
Name:Hprt1
Synonyms:Hprt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96217. Hprt.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: GO_Central
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP00493.
PaxDbiP00493.
PRIDEiP00493.

PTM databases

PhosphoSiteiP00493.

Expressioni

Gene expression databases

BgeeiP00493.
CleanExiMM_HPRT1.
GenevestigatoriP00493.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

DIPiDIP-6034N.
MINTiMINT-217744.
STRINGi10090.ENSMUSP00000026723.

Structurei

3D structure databases

ProteinModelPortaliP00493.
SMRiP00493. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
GeneTreeiENSGT00390000017323.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiP00493.
KOiK00760.
OMAiEMQWRVA.
OrthoDBiEOG7673CK.
PhylomeDBiP00493.
TreeFamiTF313367.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTRSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEYFR
210
DLNHVCVISE TGKAKYKA
Length:218
Mass (Da):24,570
Last modified:January 22, 2007 - v3
Checksum:i925CC0D4A6626E05
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011D → N in AAA96232 (PubMed:6294614).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00423 mRNA. Translation: AAA96232.1.
K01515
, K01507, K01508, K01509, K01510, K01511, K01512, K01513, K01514 Genomic DNA. Translation: AAA96271.1.
AK002286 mRNA. Translation: BAB21989.1.
AK088114 mRNA. Translation: BAC40153.1.
AK146626 mRNA. Translation: BAE27315.1.
BC083145 mRNA. Translation: AAH83145.1.
CCDSiCCDS40972.1.
PIRiI49756. RTMSG.
RefSeqiNP_038584.2. NM_013556.2.
UniGeneiMm.299381.

Genome annotation databases

EnsembliENSMUST00000026723; ENSMUSP00000026723; ENSMUSG00000025630.
GeneIDi15452.
KEGGimmu:15452.
UCSCiuc009ter.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00423 mRNA. Translation: AAA96232.1.
K01515
, K01507, K01508, K01509, K01510, K01511, K01512, K01513, K01514 Genomic DNA. Translation: AAA96271.1.
AK002286 mRNA. Translation: BAB21989.1.
AK088114 mRNA. Translation: BAC40153.1.
AK146626 mRNA. Translation: BAE27315.1.
BC083145 mRNA. Translation: AAH83145.1.
CCDSiCCDS40972.1.
PIRiI49756. RTMSG.
RefSeqiNP_038584.2. NM_013556.2.
UniGeneiMm.299381.

3D structure databases

ProteinModelPortaliP00493.
SMRiP00493. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6034N.
MINTiMINT-217744.
STRINGi10090.ENSMUSP00000026723.

Chemistry

ChEMBLiCHEMBL3243916.

PTM databases

PhosphoSiteiP00493.

Proteomic databases

MaxQBiP00493.
PaxDbiP00493.
PRIDEiP00493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026723; ENSMUSP00000026723; ENSMUSG00000025630.
GeneIDi15452.
KEGGimmu:15452.
UCSCiuc009ter.1. mouse.

Organism-specific databases

CTDi15452.
MGIiMGI:96217. Hprt.

Phylogenomic databases

eggNOGiCOG0634.
GeneTreeiENSGT00390000017323.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiP00493.
KOiK00760.
OMAiEMQWRVA.
OrthoDBiEOG7673CK.
PhylomeDBiP00493.
TreeFamiTF313367.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
ReactomeiREACT_345398. Purine salvage.

Miscellaneous databases

NextBioi288260.
PROiP00493.
SOURCEiSearch...

Gene expression databases

BgeeiP00493.
CleanExiMM_HPRT1.
GenevestigatoriP00493.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants."
    Konecki D.S., Brennand J., Fuscoe J.C., Caskey C.T., Chinault A.C.
    Nucleic Acids Res. 10:6763-6775(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene."
    Melton D.W., Konecki D.S., Brennand J., Caskey C.T.
    Proc. Natl. Acad. Sci. U.S.A. 81:2147-2151(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Myeloma.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Heart and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. Lubec G., Klug S.
    Submitted (FEB-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 74-83 AND 171-200, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-103, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHPRT_MOUSE
AccessioniPrimary (citable) accession number: P00493
Secondary accession number(s): Q545Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 20, 1986
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.