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P00484

- CAT3_ECOLX

UniProt

P00484 - CAT3_ECOLX

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Protein
Chloramphenicol acetyltransferase 3
Gene
cat3
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This enzyme is an effector of chloramphenicol resistance in bacteria.

Catalytic activityi

Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Proton acceptor

GO - Molecular functioni

  1. chloramphenicol O-acetyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Chloramphenicol acetyltransferase 3 (EC:2.3.1.28)
Alternative name(s):
Chloramphenicol acetyltransferase III
Short name:
CAT-III
Gene namesi
Name:cat3
Encoded oniPlasmid IncK R3870 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Chloramphenicol acetyltransferase 3
PRO_0000165877Add
BLAST

Proteomic databases

PRIDEiP00484.

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Helixi8 – 103
Helixi14 – 229
Beta strandi27 – 359
Helixi37 – 448
Helixi50 – 6213
Helixi66 – 683
Beta strandi69 – 735
Beta strandi76 – 816
Beta strandi84 – 918
Turni92 – 954
Beta strandi96 – 1016
Helixi108 – 12215
Beta strandi127 – 1293
Beta strandi136 – 1449
Beta strandi166 – 1705
Beta strandi173 – 1753
Beta strandi178 – 18811
Turni189 – 1913
Helixi194 – 20815

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIAX-ray2.50A1-213[»]
1CLAX-ray2.34A1-213[»]
1QCAX-ray2.20A1-213[»]
2CLAX-ray2.35A1-213[»]
3CLAX-ray1.75A1-213[»]
4CLAX-ray2.00A1-213[»]
ProteinModelPortaliP00484.
SMRiP00484. Positions 1-213.

Miscellaneous databases

EvolutionaryTraceiP00484.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
InterProiIPR023213. CAT-like_dom.
IPR018372. Chloramphenicol_AcTrfase_AS.
IPR001707. Cmp_AcTrfase.
[Graphical view]
PfamiPF00302. CAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000440. CAT. 1 hit.
ProDomiPD002660. Cmp_AcTrfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01059. CAT. 1 hit.
[Graphical view]
PROSITEiPS00100. CAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00484-1 [UniParc]FASTAAdd to Basket

« Hide

MNYTKFDVKN WVRREHFEFY RHRLPCGFSL TSKIDITTLK KSLDDSAYKF    50
YPVMIYLIAQ AVNQFDELRM AIKDDELIVW DSVDPQFTVF HQETETFSAL 100
SCPYSSDIDQ FMVNYLSVME RYKSDTKLFP QGVTPENHLN ISALPWVNFD 150
SFNLNVANFT DYFAPIITMA KYQQEGDRLL LPLSVQVHHA VCDGFHVARF 200
INRLQELCNS KLK 213
Length:213
Mass (Da):24,994
Last modified:August 1, 1988 - v1
Checksum:i4A96839AB590CB1D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07848 Genomic DNA. Translation: CAA30695.1.
PIRiA00567. XXECC3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07848 Genomic DNA. Translation: CAA30695.1 .
PIRi A00567. XXECC3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CIA X-ray 2.50 A 1-213 [» ]
1CLA X-ray 2.34 A 1-213 [» ]
1QCA X-ray 2.20 A 1-213 [» ]
2CLA X-ray 2.35 A 1-213 [» ]
3CLA X-ray 1.75 A 1-213 [» ]
4CLA X-ray 2.00 A 1-213 [» ]
ProteinModelPortali P00484.
SMRi P00484. Positions 1-213.
ModBasei Search...

Chemistry

DrugBanki DB00446. Chloramphenicol.
DB02703. Fusidic Acid.

Proteomic databases

PRIDEi P00484.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P00484.

Family and domain databases

Gene3Di 3.30.559.10. 1 hit.
InterProi IPR023213. CAT-like_dom.
IPR018372. Chloramphenicol_AcTrfase_AS.
IPR001707. Cmp_AcTrfase.
[Graphical view ]
Pfami PF00302. CAT. 1 hit.
[Graphical view ]
PIRSFi PIRSF000440. CAT. 1 hit.
ProDomi PD002660. Cmp_AcTrfase. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM01059. CAT. 1 hit.
[Graphical view ]
PROSITEi PS00100. CAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence analysis and overexpression of the gene encoding a type III chloramphenicol acetyltransferase."
    Murray I.A., Hawkins A.R., Keyte J.W., Shaw W.V.
    Biochem. J. 252:173-179(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Structure of chloramphenicol acetyltransferase at 1.75-A resolution."
    Leslie A.G.W., Moody P.C.E., Shaw W.V.
    Proc. Natl. Acad. Sci. U.S.A. 85:4133-4137(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
  3. "Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75-A resolution."
    Leslie A.G.W.
    J. Mol. Biol. 213:167-186(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).

Entry informationi

Entry nameiCAT3_ECOLX
AccessioniPrimary (citable) accession number: P00484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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