P00484 (CAT3_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloramphenicol acetyltransferase 3
EC=2.3.1.28

Alternative name(s):
Chloramphenicol acetyltransferase III
Short name=CAT-III

Gene names

Name:

cat3

Encoded on

Plasmid IncK R387

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme is an effector of chloramphenicol resistance in bacteria.
Catalytic activity	Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the chloramphenicol acetyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chloramphenicol O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

Chloramphenicol acetyltransferase 3

PRO_0000165877

Sites

Active site

189

1

Proton acceptor

Secondary structure

1

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213

Helix Strand Turn

Details...

Beta strand

3 – 5

3

Helix

8 – 10

3

Helix

14 – 22

9

Beta strand

27 – 35

9

Helix

37 – 44

8

Helix

50 – 62

13

Helix

66 – 68

3

Beta strand

69 – 73

5

Beta strand

76 – 81

6

Beta strand

84 – 91

8

Turn

92 – 95

4

Beta strand

96 – 101

6

Helix

108 – 122

15

Beta strand

127 – 129

3

Beta strand

136 – 144

9

Beta strand

166 – 170

5

Beta strand

173 – 175

3

Beta strand

178 – 188

11

Turn

189 – 191

3

Helix

194 – 208

15

Sequences

	Sequence		Length	Mass (Da)
	P00484 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: 4A96839AB590CB1D	FASTA	213	24,994
	10 20 30 40 50 60 MNYTKFDVKN WVRREHFEFY RHRLPCGFSL TSKIDITTLK KSLDDSAYKF YPVMIYLIAQ 70 80 90 100 110 120 AVNQFDELRM AIKDDELIVW DSVDPQFTVF HQETETFSAL SCPYSSDIDQ FMVNYLSVME 130 140 150 160 170 180 RYKSDTKLFP QGVTPENHLN ISALPWVNFD SFNLNVANFT DYFAPIITMA KYQQEGDRLL 190 200 210 LPLSVQVHHA VCDGFHVARF INRLQELCNS KLK

References

[1]	"Nucleotide sequence analysis and overexpression of the gene encoding a type III chloramphenicol acetyltransferase." Murray I.A., Hawkins A.R., Keyte J.W., Shaw W.V. Biochem. J. 252:173-179(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Structure of chloramphenicol acetyltransferase at 1.75-A resolution." Leslie A.G.W., Moody P.C.E., Shaw W.V. Proc. Natl. Acad. Sci. U.S.A. 85:4133-4137(1988) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
[3]	"Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75-A resolution." Leslie A.G.W. J. Mol. Biol. 213:167-186(1990) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X07848 Genomic DNA. Translation: CAA30695.1.

PIR

XXECC3. A00567.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CIA	X-ray	2.50	A	1-213	[»]
1CLA	X-ray	2.34	A	1-213	[»]
1QCA	X-ray	2.20	A	1-213	[»]
2CLA	X-ray	2.35	A	1-213	[»]
3CLA	X-ray	1.75	A	1-213	[»]
4CLA	X-ray	2.00	A	1-213	[»]

ProteinModelPortal

P00484.

SMR

P00484. Positions 1-213.

ModBase

Search...

Proteomic databases

PRIDE

P00484.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.559.10. 1 hit.

InterPro

IPR023213. CAT-like_dom.
IPR018372. Chloramphenicol_AcTrfase_AS.
IPR001707. Cmp_AcTrfase.
[Graphical view]

Pfam

PF00302. CAT. 1 hit.
[Graphical view]

PIRSF

PIRSF000440. CAT. 1 hit.

ProDom

PD002660. Cmp_AcTrfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM01059. CAT. 1 hit.
[Graphical view]

PROSITE

PS00100. CAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00446. Chloramphenicol.
DB02703. Fusidic Acid.

EvolutionaryTrace

P00484.

Entry information

Entry name

CAT3_ECOLX

Accession

Primary (citable) accession number: P00484

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1988
Last modified:	April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program