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P00484 (CAT3_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloramphenicol acetyltransferase 3

EC=2.3.1.28
Alternative name(s):
Chloramphenicol acetyltransferase III
Short name=CAT-III
Gene names
Name:cat3
Encoded onPlasmid IncK R387
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme is an effector of chloramphenicol resistance in bacteria.

Catalytic activity

Acetyl-CoA + chloramphenicol = CoA + chloramphenicol 3-acetate.

Subunit structure

Homotrimer.

Sequence similarities

Belongs to the chloramphenicol acetyltransferase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Molecular functionAcyltransferase
Transferase
   Technical term3D-structure
Direct protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processresponse to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionchloramphenicol O-acetyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Chloramphenicol acetyltransferase 3
PRO_0000165877

Sites

Active site1891Proton acceptor

Secondary structure

..................................... 213
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00484 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 4A96839AB590CB1D

FASTA21324,994
        10         20         30         40         50         60 
MNYTKFDVKN WVRREHFEFY RHRLPCGFSL TSKIDITTLK KSLDDSAYKF YPVMIYLIAQ 

        70         80         90        100        110        120 
AVNQFDELRM AIKDDELIVW DSVDPQFTVF HQETETFSAL SCPYSSDIDQ FMVNYLSVME 

       130        140        150        160        170        180 
RYKSDTKLFP QGVTPENHLN ISALPWVNFD SFNLNVANFT DYFAPIITMA KYQQEGDRLL 

       190        200        210 
LPLSVQVHHA VCDGFHVARF INRLQELCNS KLK 

« Hide

References

[1]"Nucleotide sequence analysis and overexpression of the gene encoding a type III chloramphenicol acetyltransferase."
Murray I.A., Hawkins A.R., Keyte J.W., Shaw W.V.
Biochem. J. 252:173-179(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Structure of chloramphenicol acetyltransferase at 1.75-A resolution."
Leslie A.G.W., Moody P.C.E., Shaw W.V.
Proc. Natl. Acad. Sci. U.S.A. 85:4133-4137(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
[3]"Refined crystal structure of type III chloramphenicol acetyltransferase at 1.75-A resolution."
Leslie A.G.W.
J. Mol. Biol. 213:167-186(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07848 Genomic DNA. Translation: CAA30695.1.
PIRXXECC3. A00567.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIAX-ray2.50A1-213[»]
1CLAX-ray2.34A1-213[»]
1QCAX-ray2.20A1-213[»]
2CLAX-ray2.35A1-213[»]
3CLAX-ray1.75A1-213[»]
4CLAX-ray2.00A1-213[»]
ProteinModelPortalP00484.
SMRP00484. Positions 1-213.
ModBaseSearch...
MobiDBSearch...

Chemistry

DrugBankDB00446. Chloramphenicol.
DB02703. Fusidic Acid.

Proteomic databases

PRIDEP00484.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.559.10. 1 hit.
InterProIPR023213. CAT-like_dom.
IPR018372. Chloramphenicol_AcTrfase_AS.
IPR001707. Cmp_AcTrfase.
[Graphical view]
PfamPF00302. CAT. 1 hit.
[Graphical view]
PIRSFPIRSF000440. CAT. 1 hit.
ProDomPD002660. Cmp_AcTrfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM01059. CAT. 1 hit.
[Graphical view]
PROSITEPS00100. CAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00484.

Entry information

Entry nameCAT3_ECOLX
AccessionPrimary (citable) accession number: P00484
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references