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Protein

Modification methylase SPRI

Gene
N/A
Organism
Bacillus phage SPR (Bacteriophage SPR)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme methylates the 5'cytosine in the sequence GGCC and both cytosines in the sequence CCGG.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi2834 M.SPRI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SPRI (EC:2.1.1.37)
Short name:
M.SPRI
Alternative name(s):
Cytosine-specific methyltransferase SPRI
OrganismiBacillus phage SPR (Bacteriophage SPR)
Taxonomic identifieri10725 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdavirusunclassified Lambda-like viruses
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878681 – 439Modification methylase SPRIAdd BLAST439

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP00476
SMRiP00476
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 436SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST433

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 3 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P00476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKLRVMSLF SGIGAFEAAL RNIGVGYELV GFSEIDKYAV KSFCAIHNVD
60 70 80 90 100
EQLNFGDVSK IDKKKLPEFD LLVGGSPCQS FSVAGHRKGF EDTRGTLFFQ
110 120 130 140 150
YVETLKEKQP KFFVFENVKG LINHDKGNTL NVMAEAFSEV GYRIDLELLN
160 170 180 190 200
SKFFNVPQNR ERLYIIGIRE DLIKNEEWSL DFKRKDILQK GKQRLVELDI
210 220 230 240 250
KSFNFRWTAQ SAATKRLKDL LEEYVDEKYY LNEDKTNSLI KELSTSRLNE
260 270 280 290 300
NLTVEQVGNI NPSGNGMNGN VYNSSGLSPT ITTNKGEGLK IAVEYSRKSG
310 320 330 340 350
LGRELAVSHT LSASDWRGLN RNQKQNAVVE VRPVLTPERG EKRQNGRRFK
360 370 380 390 400
DDGEPAFTVN TIDRHGVAVG EYPKYRIRRL TPLECFRLQA FDDEDFEKAF
410 420 430
AAGISNSQLY KQTGNSITVT VLESIFKELI HTYINKESE
Length:439
Mass (Da):49,881
Last modified:July 21, 1986 - v1
Checksum:i407DCF178D32D5BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti413T → A in AAA32604 (PubMed:6096817).Curated1
Sequence conflicti434I → V in AAA32604 (PubMed:6096817).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02124 Genomic DNA Translation: AAA32604.1
PIRiA91516 CTBPSR

Similar proteinsi

Entry informationi

Entry nameiMTBS_BPSPR
AccessioniPrimary (citable) accession number: P00476
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health