Reviewed, UniProtKB/Swiss-Prot P00476 (MTBS_BPSPR)

Names and origin

Protein names	Recommended name:     Modification methylase SPRI       Short name=M.SPRI     EC=2.1.1.37 Alternative name(s):     Cytosine-specific methyltransferase SPRI
Organism	Bacillus phage SPR (Bacteriophage SPR)
Taxonomic identifier	10725 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses › unclassified Lambda-like viruses
Virus host	Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme methylates the 5'cytosine in the sequence GGCC and both cytosines in the sequence CCGG.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Modification methylase SPRI		PRO_0000087868
Sites
	Active site	78	1	By similarity
Experimental info
	Sequence conflict	413	1	T → A in AAA32604. Ref.2
	Sequence conflict	434	1	I → V in AAA32604. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00476-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 407DCF178D32D5BE	FASTA	439	49,881
	10 20 30 40 50 60 MGKLRVMSLF SGIGAFEAAL RNIGVGYELV GFSEIDKYAV KSFCAIHNVD EQLNFGDVSK 70 80 90 100 110 120 IDKKKLPEFD LLVGGSPCQS FSVAGHRKGF EDTRGTLFFQ YVETLKEKQP KFFVFENVKG 130 140 150 160 170 180 LINHDKGNTL NVMAEAFSEV GYRIDLELLN SKFFNVPQNR ERLYIIGIRE DLIKNEEWSL 190 200 210 220 230 240 DFKRKDILQK GKQRLVELDI KSFNFRWTAQ SAATKRLKDL LEEYVDEKYY LNEDKTNSLI 250 260 270 280 290 300 KELSTSRLNE NLTVEQVGNI NPSGNGMNGN VYNSSGLSPT ITTNKGEGLK IAVEYSRKSG 310 320 330 340 350 360 LGRELAVSHT LSASDWRGLN RNQKQNAVVE VRPVLTPERG EKRQNGRRFK DDGEPAFTVN 370 380 390 400 410 420 TIDRHGVAVG EYPKYRIRRL TPLECFRLQA FDDEDFEKAF AAGISNSQLY KQTGNSITVT 430 VLESIFKELI HTYINKESE

References

[1]	"Restriction and modification in Bacillus subtilis: nucleotide sequence, functional organization and product of the DNA methyltransferase gene of bacteriophage SPR." Buhk H.-J., Behrens B., Tailor R., Wilke K., Prada J.J., Gunthert U., Noyer-Weidner M., Jentsch S., Trautner T.A. Gene 29:51-61(1984) [PubMed: 6092231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR." Posfai G., Baldauf F., Erdei S., Posfai J., Venetianer P., Kiss A. Nucleic Acids Res. 12:9039-9049(1984) [PubMed: 6096817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	K02124 Genomic DNA. Translation: AAA32604.1.
PIR	CTBPSR. A91516.
3D structure databases
HSSP	HSSP built from PDB template 6MHT based on UniProtKB P05102.
ModBase	Search...
Protein family/group databases
REBASE	2834. M.SPRI.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTBS_BPSPR

Accession

Primary (citable) accession number: P00476

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	March 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)