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P00476

- MTBS_BPSPR

UniProt

P00476 - MTBS_BPSPR

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Protein

Modification methylase SPRI

Gene
N/A
Organism
Bacillus phage SPR (Bacteriophage SPR)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This enzyme methylates the 5'cytosine in the sequence GGCC and both cytosines in the sequence CCGG.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi2834. M.SPRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase SPRI (EC:2.1.1.37)
Short name:
M.SPRI
Alternative name(s):
Cytosine-specific methyltransferase SPRI
OrganismiBacillus phage SPR (Bacteriophage SPR)
Taxonomic identifieri10725 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostiBacillus subtilis [TaxID: 1423]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Modification methylase SPRIPRO_0000087868Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP00476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 436433SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00476-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKLRVMSLF SGIGAFEAAL RNIGVGYELV GFSEIDKYAV KSFCAIHNVD
60 70 80 90 100
EQLNFGDVSK IDKKKLPEFD LLVGGSPCQS FSVAGHRKGF EDTRGTLFFQ
110 120 130 140 150
YVETLKEKQP KFFVFENVKG LINHDKGNTL NVMAEAFSEV GYRIDLELLN
160 170 180 190 200
SKFFNVPQNR ERLYIIGIRE DLIKNEEWSL DFKRKDILQK GKQRLVELDI
210 220 230 240 250
KSFNFRWTAQ SAATKRLKDL LEEYVDEKYY LNEDKTNSLI KELSTSRLNE
260 270 280 290 300
NLTVEQVGNI NPSGNGMNGN VYNSSGLSPT ITTNKGEGLK IAVEYSRKSG
310 320 330 340 350
LGRELAVSHT LSASDWRGLN RNQKQNAVVE VRPVLTPERG EKRQNGRRFK
360 370 380 390 400
DDGEPAFTVN TIDRHGVAVG EYPKYRIRRL TPLECFRLQA FDDEDFEKAF
410 420 430
AAGISNSQLY KQTGNSITVT VLESIFKELI HTYINKESE
Length:439
Mass (Da):49,881
Last modified:July 21, 1986 - v1
Checksum:i407DCF178D32D5BE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti413 – 4131T → A in AAA32604. (PubMed:6096817)Curated
Sequence conflicti434 – 4341I → V in AAA32604. (PubMed:6096817)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02124 Genomic DNA. Translation: AAA32604.1.
PIRiA91516. CTBPSR.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02124 Genomic DNA. Translation: AAA32604.1 .
PIRi A91516. CTBPSR.

3D structure databases

ProteinModelPortali P00476.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 2834. M.SPRI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Restriction and modification in Bacillus subtilis: nucleotide sequence, functional organization and product of the DNA methyltransferase gene of bacteriophage SPR."
    Buhk H.-J., Behrens B., Tailor R., Wilke K., Prada J.J., Gunthert U., Noyer-Weidner M., Jentsch S., Trautner T.A.
    Gene 29:51-61(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR."
    Posfai G., Baldauf F., Erdei S., Posfai J., Venetianer P., Kiss A.
    Nucleic Acids Res. 12:9039-9049(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTBS_BPSPR
AccessioniPrimary (citable) accession number: P00476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3