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P00476 (MTBS_BPSPR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase SPRI

Short name=M.SPRI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase SPRI
OrganismBacillus phage SPR (Bacteriophage SPR)
Taxonomic identifier10725 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirusunclassified Lambda-like viruses
Virus hostBacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme methylates the 5'cytosine in the sequence GGCC and both cytosines in the sequence CCGG.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439Modification methylase SPRI
PRO_0000087868

Regions

Domain4 – 436433SAM-dependent MTase C5-type

Sites

Active site781 By similarity

Experimental info

Sequence conflict4131T → A in AAA32604. Ref.2
Sequence conflict4341I → V in AAA32604. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P00476 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 407DCF178D32D5BE

FASTA43949,881
        10         20         30         40         50         60 
MGKLRVMSLF SGIGAFEAAL RNIGVGYELV GFSEIDKYAV KSFCAIHNVD EQLNFGDVSK 

        70         80         90        100        110        120 
IDKKKLPEFD LLVGGSPCQS FSVAGHRKGF EDTRGTLFFQ YVETLKEKQP KFFVFENVKG 

       130        140        150        160        170        180 
LINHDKGNTL NVMAEAFSEV GYRIDLELLN SKFFNVPQNR ERLYIIGIRE DLIKNEEWSL 

       190        200        210        220        230        240 
DFKRKDILQK GKQRLVELDI KSFNFRWTAQ SAATKRLKDL LEEYVDEKYY LNEDKTNSLI 

       250        260        270        280        290        300 
KELSTSRLNE NLTVEQVGNI NPSGNGMNGN VYNSSGLSPT ITTNKGEGLK IAVEYSRKSG 

       310        320        330        340        350        360 
LGRELAVSHT LSASDWRGLN RNQKQNAVVE VRPVLTPERG EKRQNGRRFK DDGEPAFTVN 

       370        380        390        400        410        420 
TIDRHGVAVG EYPKYRIRRL TPLECFRLQA FDDEDFEKAF AAGISNSQLY KQTGNSITVT 

       430 
VLESIFKELI HTYINKESE 

« Hide

References

[1]"Restriction and modification in Bacillus subtilis: nucleotide sequence, functional organization and product of the DNA methyltransferase gene of bacteriophage SPR."
Buhk H.-J., Behrens B., Tailor R., Wilke K., Prada J.J., Gunthert U., Noyer-Weidner M., Jentsch S., Trautner T.A.
Gene 29:51-61(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR."
Posfai G., Baldauf F., Erdei S., Posfai J., Venetianer P., Kiss A.
Nucleic Acids Res. 12:9039-9049(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02124 Genomic DNA. Translation: AAA32604.1.
PIRCTBPSR. A91516.

3D structure databases

ProteinModelPortalP00476.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2834. M.SPRI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBS_BPSPR
AccessionPrimary (citable) accession number: P00476
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries