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P00474 (MTPS_PROST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase PstI

Short name=M.PstI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase PstI
Gene names
Name:pstIM
OrganismProvidencia stuartii
Taxonomic identifier588 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 507507Modification methylase PstI
PRO_0000087978

Sequences

Sequence LengthMass (Da)Tools
P00474 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: AF5DA9341741823D

FASTA50756,877
        10         20         30         40         50         60 
MTEAATQLPI SLNILVDSIR EAANSTLDET LRSKLGQFMS SSAVSELMAN LFESYVGEHE 

        70         80         90        100        110        120 
ILDAGAGVGS LTAAFVQNAT LNGAKSISST CYEISEVMVY NLIQVLDLCK IRAMEFEVNW 

       130        140        150        160        170        180 
QQKIIESDFI QASVEQLLIE NYSPKYNKAI LNPPYLKIAA KGRERALLQK VGIEASNLYS 

       190        200        210        220        230        240 
AFVALAIKQL KSGGELVAIT PRSFCNGPYF NDFRKQMLDE CSLNKIHVFN SRKSAFKADN 

       250        260        270        280        290        300 
VLQENIIYHL TKGETQRKVV TVYSSTCAND INPTIFEVPF DEIVKSNNPD LFIHIVTNEQ 

       310        320        330        340        350        360 
ERELANKAGG LPCSLSDLGI QVSTGKVVDF RTRENLSMEY ISNSVPLIFP QHLQRCSIVW 

       370        380        390        400        410        420 
PITKAKKPNA LIVNEATNNL MVPNGIYVLT RRLTAKEEKR RIVASIYYPD IANVDTVGFD 

       430        440        450        460        470        480 
NKINYFHANG KPLDISLAKG LWVFLNSTLI DKYFRQMNGH TQVNATDLRA LRYPTREQLE 

       490        500 
DIANQVDFGE FEQTKIDEII NQSLQLM 

« Hide

References

[1]"The organization and complete nucleotide sequence of the PstI restriction-modification system."
Walder R.Y., Walder J.A., Donelson J.E.
J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 164.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02081 Genomic DNA. Translation: AAA25672.1.
PIRXYOFS. A00553.

3D structure databases

ProteinModelPortalP00474.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3483. M.PstI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTPS_PROST
AccessionPrimary (citable) accession number: P00474
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries