P00474 (MTPS_PROST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase PstI
Short name=M.PstI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase PstI

Gene names

Name:

pstIM

Organism

Providencia stuartii

Taxonomic identifier

588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Providencia

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Modification methylase PstI		PRO_0000087978

Sequences

	Sequence		Length	Mass (Da)
	P00474 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: AF5DA9341741823D	FASTA	507	56,877
	10 20 30 40 50 60 MTEAATQLPI SLNILVDSIR EAANSTLDET LRSKLGQFMS SSAVSELMAN LFESYVGEHE 70 80 90 100 110 120 ILDAGAGVGS LTAAFVQNAT LNGAKSISST CYEISEVMVY NLIQVLDLCK IRAMEFEVNW 130 140 150 160 170 180 QQKIIESDFI QASVEQLLIE NYSPKYNKAI LNPPYLKIAA KGRERALLQK VGIEASNLYS 190 200 210 220 230 240 AFVALAIKQL KSGGELVAIT PRSFCNGPYF NDFRKQMLDE CSLNKIHVFN SRKSAFKADN 250 260 270 280 290 300 VLQENIIYHL TKGETQRKVV TVYSSTCAND INPTIFEVPF DEIVKSNNPD LFIHIVTNEQ 310 320 330 340 350 360 ERELANKAGG LPCSLSDLGI QVSTGKVVDF RTRENLSMEY ISNSVPLIFP QHLQRCSIVW 370 380 390 400 410 420 PITKAKKPNA LIVNEATNNL MVPNGIYVLT RRLTAKEEKR RIVASIYYPD IANVDTVGFD 430 440 450 460 470 480 NKINYFHANG KPLDISLAKG LWVFLNSTLI DKYFRQMNGH TQVNATDLRA LRYPTREQLE 490 500 DIANQVDFGE FEQTKIDEII NQSLQLM

References

[1]	"The organization and complete nucleotide sequence of the PstI restriction-modification system." Walder R.Y., Walder J.A., Donelson J.E. J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 164.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02081 Genomic DNA. Translation: AAA25672.1.
PIR	XYOFS. A00553.
3D structure databases
ProteinModelPortal	P00474.
ModBase	Search...
Protein family/group databases
REBASE	3483. M.PstI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002296. N12N6_MeTrfase. IPR011639. RM_methylase_Eco57I. [Graphical view]
Pfam	PF07669. Eco57I. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTPS_PROST

Accession

Primary (citable) accession number: P00474

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program