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P00474

- MTPS_PROST

UniProt

P00474 - MTPS_PROST

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Protein

Modification methylase PstI

Gene
pstIM
Organism
Providencia stuartii
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3483. M.PstI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PstI (EC:2.1.1.72)
Short name:
M.PstI
Alternative name(s):
Adenine-specific methyltransferase PstI
Gene namesi
Name:pstIM
OrganismiProvidencia stuartii
Taxonomic identifieri588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Modification methylase PstIPRO_0000087978Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP00474.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00474-1 [UniParc]FASTAAdd to Basket

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MTEAATQLPI SLNILVDSIR EAANSTLDET LRSKLGQFMS SSAVSELMAN    50
LFESYVGEHE ILDAGAGVGS LTAAFVQNAT LNGAKSISST CYEISEVMVY 100
NLIQVLDLCK IRAMEFEVNW QQKIIESDFI QASVEQLLIE NYSPKYNKAI 150
LNPPYLKIAA KGRERALLQK VGIEASNLYS AFVALAIKQL KSGGELVAIT 200
PRSFCNGPYF NDFRKQMLDE CSLNKIHVFN SRKSAFKADN VLQENIIYHL 250
TKGETQRKVV TVYSSTCAND INPTIFEVPF DEIVKSNNPD LFIHIVTNEQ 300
ERELANKAGG LPCSLSDLGI QVSTGKVVDF RTRENLSMEY ISNSVPLIFP 350
QHLQRCSIVW PITKAKKPNA LIVNEATNNL MVPNGIYVLT RRLTAKEEKR 400
RIVASIYYPD IANVDTVGFD NKINYFHANG KPLDISLAKG LWVFLNSTLI 450
DKYFRQMNGH TQVNATDLRA LRYPTREQLE DIANQVDFGE FEQTKIDEII 500
NQSLQLM 507
Length:507
Mass (Da):56,877
Last modified:July 21, 1986 - v1
Checksum:iAF5DA9341741823D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02081 Genomic DNA. Translation: AAA25672.1.
PIRiA00553. XYOFS.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02081 Genomic DNA. Translation: AAA25672.1 .
PIRi A00553. XYOFS.

3D structure databases

ProteinModelPortali P00474.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3483. M.PstI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The organization and complete nucleotide sequence of the PstI restriction-modification system."
    Walder R.Y., Walder J.A., Donelson J.E.
    J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 164.

Entry informationi

Entry nameiMTPS_PROST
AccessioniPrimary (citable) accession number: P00474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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