Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00474

- MTPS_PROST

UniProt

P00474 - MTPS_PROST

Protein

Modification methylase PstI

Gene

pstIM

Organism
Providencia stuartii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3483. M.PstI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase PstI (EC:2.1.1.72)
    Short name:
    M.PstI
    Alternative name(s):
    Adenine-specific methyltransferase PstI
    Gene namesi
    Name:pstIM
    OrganismiProvidencia stuartii
    Taxonomic identifieri588 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProvidencia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 507507Modification methylase PstIPRO_0000087978Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP00474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00474-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEAATQLPI SLNILVDSIR EAANSTLDET LRSKLGQFMS SSAVSELMAN    50
    LFESYVGEHE ILDAGAGVGS LTAAFVQNAT LNGAKSISST CYEISEVMVY 100
    NLIQVLDLCK IRAMEFEVNW QQKIIESDFI QASVEQLLIE NYSPKYNKAI 150
    LNPPYLKIAA KGRERALLQK VGIEASNLYS AFVALAIKQL KSGGELVAIT 200
    PRSFCNGPYF NDFRKQMLDE CSLNKIHVFN SRKSAFKADN VLQENIIYHL 250
    TKGETQRKVV TVYSSTCAND INPTIFEVPF DEIVKSNNPD LFIHIVTNEQ 300
    ERELANKAGG LPCSLSDLGI QVSTGKVVDF RTRENLSMEY ISNSVPLIFP 350
    QHLQRCSIVW PITKAKKPNA LIVNEATNNL MVPNGIYVLT RRLTAKEEKR 400
    RIVASIYYPD IANVDTVGFD NKINYFHANG KPLDISLAKG LWVFLNSTLI 450
    DKYFRQMNGH TQVNATDLRA LRYPTREQLE DIANQVDFGE FEQTKIDEII 500
    NQSLQLM 507
    Length:507
    Mass (Da):56,877
    Last modified:July 21, 1986 - v1
    Checksum:iAF5DA9341741823D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02081 Genomic DNA. Translation: AAA25672.1.
    PIRiA00553. XYOFS.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02081 Genomic DNA. Translation: AAA25672.1 .
    PIRi A00553. XYOFS.

    3D structure databases

    ProteinModelPortali P00474.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3483. M.PstI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization and complete nucleotide sequence of the PstI restriction-modification system."
      Walder R.Y., Walder J.A., Donelson J.E.
      J. Biol. Chem. 259:8015-8026(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 164.

    Entry informationi

    Entry nameiMTPS_PROST
    AccessioniPrimary (citable) accession number: P00474
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3