Reviewed, UniProtKB/Swiss-Prot P00473 (MTH2_HAEPH)

Names and origin

Protein names

Recommended name:
    Modification methylase HhaII
      Short name=M.HhaII
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase HhaII

Gene names

Name:

hhaIIM

Organism

Haemophilus parahaemolyticus

Taxonomic identifier

735 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.
Caution	Strain ATCC 10014 was originally thought to originate from H.haemolyticus.
Sequence caution	The sequence K00508 differs from that shown. Reason: Miscellaneous discrepancy.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Modification methylase HhaII		PRO_0000087972

Sequences

	Sequence		Length	Mass (Da)
	P00473-1 [UniParc]. Last modified February 1, 1991. Version 2. Checksum: FABEEED916927505	FASTA	228	26,432
	10 20 30 40 50 60 MFSHQDYLSF VNKKNKMNGI DLFKLIPDKA VKIAFFDPQY RGVLDKMSYG NEGKGRGKER 70 80 90 100 110 120 AALPQMTDEI IQQFINEFER VLLPNGYLFL WVDKFHLVEG VKPWLENTPS LSVVDMLTWD 130 140 150 160 170 180 KQKIGMGYRT RRRSEYLVVI QKEPKKAKIT WTLHNIPDVW AEKLQSKPHT HSKPIEMQKQ 190 200 210 220 LILATTQEGD LILDPASGGY SVFECCKQTN RNFIGCDLIF GDDENEQD

References

[1]	"Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus." Chandrasegaran S., Wu L.P., Valda E., Smith H.O. Gene 74:15-21(1988) [PubMed: 3248721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
[2]	"The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus." Schoner B., Kelly S., Smith H.O. Gene 24:227-236(1983) [PubMed: 6315538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M24624 Genomic DNA. Translation: AAA24963.1. K00508 Genomic DNA. No translation available.
PIR	XYHIH2. JS0103.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.72. 39513.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH2_HAEPH

Accession

Primary (citable) accession number: P00473

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 1, 1991
Last modified:	September 22, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)