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Protein

Modification methylase HhaII

Gene

hhaIIM

Organism
Haemophilus parahaemolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3422. M.HhaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HhaII (EC:2.1.1.72)
Short name:
M.HhaII
Alternative name(s):
Adenine-specific methyltransferase HhaII
Gene namesi
Name:hhaIIM
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Modification methylase HhaIIPRO_0000087972Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP00473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P00473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSHQDYLSF VNKKNKMNGI DLFKLIPDKA VKIAFFDPQY RGVLDKMSYG
60 70 80 90 100
NEGKGRGKER AALPQMTDEI IQQFINEFER VLLPNGYLFL WVDKFHLVEG
110 120 130 140 150
VKPWLENTPS LSVVDMLTWD KQKIGMGYRT RRRSEYLVVI QKEPKKAKIT
160 170 180 190 200
WTLHNIPDVW AEKLQSKPHT HSKPIEMQKQ LILATTQEGD LILDPASGGY
210 220
SVFECCKQTN RNFIGCDLIF GDDENEQD
Length:228
Mass (Da):26,432
Last modified:February 1, 1991 - v2
Checksum:iFABEEED916927505
GO

Sequence cautioni

The sequence K00508 differs from that shown.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24624 Genomic DNA. Translation: AAA24963.1.
K00508 Genomic DNA. No translation available.
PIRiJS0103. XYHIH2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24624 Genomic DNA. Translation: AAA24963.1.
K00508 Genomic DNA. No translation available.
PIRiJS0103. XYHIH2.

3D structure databases

ProteinModelPortaliP00473.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3422. M.HhaII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
    Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
    Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
  2. "The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
    Schoner B., Kelly S., Smith H.O.
    Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Entry informationi

Entry nameiMTH2_HAEPH
AccessioniPrimary (citable) accession number: P00473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: January 7, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain ATCC 10014 was originally thought to originate from H.haemolyticus.Curated

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.