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P00473

- MTH2_HAEPH

UniProt

P00473 - MTH2_HAEPH

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Protein

Modification methylase HhaII

Gene
hhaIIM
Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3422. M.HhaII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HhaII (EC:2.1.1.72)
Short name:
M.HhaII
Alternative name(s):
Adenine-specific methyltransferase HhaII
Gene namesi
Name:hhaIIM
OrganismiHaemophilus parahaemolyticus
Taxonomic identifieri735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Modification methylase HhaIIPRO_0000087972Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP00473.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P00473-1 [UniParc]FASTAAdd to Basket

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MFSHQDYLSF VNKKNKMNGI DLFKLIPDKA VKIAFFDPQY RGVLDKMSYG    50
NEGKGRGKER AALPQMTDEI IQQFINEFER VLLPNGYLFL WVDKFHLVEG 100
VKPWLENTPS LSVVDMLTWD KQKIGMGYRT RRRSEYLVVI QKEPKKAKIT 150
WTLHNIPDVW AEKLQSKPHT HSKPIEMQKQ LILATTQEGD LILDPASGGY 200
SVFECCKQTN RNFIGCDLIF GDDENEQD 228
Length:228
Mass (Da):26,432
Last modified:February 1, 1991 - v2
Checksum:iFABEEED916927505
GO

Sequence cautioni

The sequence K00508 differs from that shown. Reason:

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24624 Genomic DNA. Translation: AAA24963.1.
K00508 Genomic DNA. No translation available.
PIRiJS0103. XYHIH2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M24624 Genomic DNA. Translation: AAA24963.1 .
K00508 Genomic DNA. No translation available.
PIRi JS0103. XYHIH2.

3D structure databases

ProteinModelPortali P00473.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3422. M.HhaII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
    Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
    Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
  2. "The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
    Schoner B., Kelly S., Smith H.O.
    Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Entry informationi

Entry nameiMTH2_HAEPH
AccessioniPrimary (citable) accession number: P00473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain ATCC 10014 was originally thought to originate from H.haemolyticus.

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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