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P00473

- MTH2_HAEPH

UniProt

P00473 - MTH2_HAEPH

Protein

Modification methylase HhaII

Gene

hhaIIM

Organism
Haemophilus parahaemolyticus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (01 Feb 1991)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3422. M.HhaII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase HhaII (EC:2.1.1.72)
    Short name:
    M.HhaII
    Alternative name(s):
    Adenine-specific methyltransferase HhaII
    Gene namesi
    Name:hhaIIM
    OrganismiHaemophilus parahaemolyticus
    Taxonomic identifieri735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228Modification methylase HhaIIPRO_0000087972Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP00473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00506. D21N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P00473-1 [UniParc]FASTAAdd to Basket

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    MFSHQDYLSF VNKKNKMNGI DLFKLIPDKA VKIAFFDPQY RGVLDKMSYG    50
    NEGKGRGKER AALPQMTDEI IQQFINEFER VLLPNGYLFL WVDKFHLVEG 100
    VKPWLENTPS LSVVDMLTWD KQKIGMGYRT RRRSEYLVVI QKEPKKAKIT 150
    WTLHNIPDVW AEKLQSKPHT HSKPIEMQKQ LILATTQEGD LILDPASGGY 200
    SVFECCKQTN RNFIGCDLIF GDDENEQD 228
    Length:228
    Mass (Da):26,432
    Last modified:February 1, 1991 - v2
    Checksum:iFABEEED916927505
    GO

    Sequence cautioni

    The sequence K00508 differs from that shown. Reason:

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24624 Genomic DNA. Translation: AAA24963.1.
    K00508 Genomic DNA. No translation available.
    PIRiJS0103. XYHIH2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24624 Genomic DNA. Translation: AAA24963.1 .
    K00508 Genomic DNA. No translation available.
    PIRi JS0103. XYHIH2.

    3D structure databases

    ProteinModelPortali P00473.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3422. M.HhaII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00506. D21N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
      Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
      Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
    2. "The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
      Schoner B., Kelly S., Smith H.O.
      Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

    Entry informationi

    Entry nameiMTH2_HAEPH
    AccessioniPrimary (citable) accession number: P00473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: February 1, 1991
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Strain ATCC 10014 was originally thought to originate from H.haemolyticus.Curated

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3