Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P00473 (MTH2_HAEPH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HhaII

Short name=M.HhaII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase HhaII
Gene names
Name:hhaIIM
OrganismHaemophilus parahaemolyticus
Taxonomic identifier735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HhaII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Caution

Strain ATCC 10014 was originally thought to originate from H.haemolyticus.

Sequence caution

The sequence K00508 differs from that shown. Reason:

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228Modification methylase HhaII
PRO_0000087972

Sequences

Sequence LengthMass (Da)Tools
P00473 [UniParc].

Last modified February 1, 1991. Version 2.
Checksum: FABEEED916927505

FASTA22826,432
        10         20         30         40         50         60 
MFSHQDYLSF VNKKNKMNGI DLFKLIPDKA VKIAFFDPQY RGVLDKMSYG NEGKGRGKER 

        70         80         90        100        110        120 
AALPQMTDEI IQQFINEFER VLLPNGYLFL WVDKFHLVEG VKPWLENTPS LSVVDMLTWD 

       130        140        150        160        170        180 
KQKIGMGYRT RRRSEYLVVI QKEPKKAKIT WTLHNIPDVW AEKLQSKPHT HSKPIEMQKQ 

       190        200        210        220 
LILATTQEGD LILDPASGGY SVFECCKQTN RNFIGCDLIF GDDENEQD 

« Hide

References

[1]"Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus."
Chandrasegaran S., Wu L.P., Valda E., Smith H.O.
Gene 74:15-21(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.
[2]"The nucleotide sequence of the HhaII restriction and modification genes from Haemophilus haemolyticus."
Schoner B., Kelly S., Smith H.O.
Gene 24:227-236(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10014 / CCUG 3716 / NCTC 8479.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24624 Genomic DNA. Translation: AAA24963.1.
K00508 Genomic DNA. No translation available.
PIRXYHIH2. JS0103.

3D structure databases

ProteinModelPortalP00473.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3422. M.HhaII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00506. D21N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTH2_HAEPH
AccessionPrimary (citable) accession number: P00473
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: February 1, 1991
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries