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P00472

- MTE1_ECOLX

UniProt

P00472 - MTE1_ECOLX

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Protein

Modification methylase EcoRI

Gene
ecoRIM
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3395. M.EcoRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRI (EC:2.1.1.72)
Short name:
M.EcoRI
Alternative name(s):
Adenine-specific methyltransferase EcoRI
Gene namesi
Name:ecoRIM
Encoded oniPlasmid pMB11 Publication
Plasmid pMB41 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 326325Modification methylase EcoRIPRO_0000087959Add
BLAST

Proteomic databases

PRIDEiP00472.

Interactioni

Subunit structurei

Monomer.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR025247. EcoRI_methylase.
[Graphical view]
PfamiPF13651. EcoRI_methylase. 1 hit.
[Graphical view]
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00472-1 [UniParc]FASTAAdd to Basket

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MARNATNKLL HKAKKSKSDE FYTQYCDIEN ELQYYREHFS DKVVYCNCDD    50
PRVSNFFKYF AVNFDNLGLK KLIASCYVEN KEGFSSSEAA KNGFYYEYHK 100
ENGKKLVFDD ISVSSFCGDG DFRSSESIDL LKKSDIVVTN PPFSLFREYL 150
DQLIKYDKKF LIIANVNSIT YKEVFNLIKE NKIWLGVHLG RGVSGFIVPE 200
HYELYGTEAR IDSNGNRIIS PNNCLWLTNL DVFIRHKDLP LTRKYFGNES 250
SYPKYDNYDA INVNKTKDIP LDYNGVMGVP ITFLHKFNPE QFELIKFRKG 300
VDEKDLSING KCPYFRILIK NKRLQK 326
Length:326
Mass (Da):38,044
Last modified:January 23, 2007 - v2
Checksum:i5F6AFE1DD1CBB1A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01675 Genomic DNA. Translation: AAA26372.1.
PIRiA92308. XYECP4.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01675 Genomic DNA. Translation: AAA26372.1 .
PIRi A92308. XYECP4.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3395. M.EcoRI.

Proteomic databases

PRIDEi P00472.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR025247. EcoRI_methylase.
[Graphical view ]
Pfami PF13651. EcoRI_methylase. 1 hit.
[Graphical view ]
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase."
    Greene P.J., Gupta M., Boyer H.W., Brown W.E., Rosenberg J.M.
    J. Biol. Chem. 256:2143-2153(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pMB1
  2. "DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes."
    Newman A.K., Rubin R.A., Kim S.-H., Modrich P.
    J. Biol. Chem. 256:2131-2139(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C.
    Plasmid: pMB4
  3. "Partial NH2- and COOH-terminal sequence analyses of Eco RI DNA restriction and modification enzymes."
    Rubin R.A., Modrich P., Vanaman T.C.
    J. Biol. Chem. 256:2140-2142(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: CONFIRMATION OF AMINO AND CARBOXYL ENDS OF SEQUENCE BY AMINO ACID ANALYSIS.

Entry informationi

Entry nameiMTE1_ECOLX
AccessioniPrimary (citable) accession number: P00472
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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