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Protein

Modification methylase EcoRI

Gene

ecoRIM

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3395 M.EcoRI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRI (EC:2.1.1.72)
Short name:
M.EcoRI
Alternative name(s):
Adenine-specific methyltransferase EcoRI
Gene namesi
Name:ecoRIM
Encoded oniPlasmid pMB11 Publication
Plasmid pMB41 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000879592 – 326Modification methylase EcoRIAdd BLAST325

Interactioni

Subunit structurei

Monomer.

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR025247 EcoRI_methylase
PfamiView protein in Pfam
PF13651 EcoRI_methylase, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00472-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARNATNKLL HKAKKSKSDE FYTQYCDIEN ELQYYREHFS DKVVYCNCDD
60 70 80 90 100
PRVSNFFKYF AVNFDNLGLK KLIASCYVEN KEGFSSSEAA KNGFYYEYHK
110 120 130 140 150
ENGKKLVFDD ISVSSFCGDG DFRSSESIDL LKKSDIVVTN PPFSLFREYL
160 170 180 190 200
DQLIKYDKKF LIIANVNSIT YKEVFNLIKE NKIWLGVHLG RGVSGFIVPE
210 220 230 240 250
HYELYGTEAR IDSNGNRIIS PNNCLWLTNL DVFIRHKDLP LTRKYFGNES
260 270 280 290 300
SYPKYDNYDA INVNKTKDIP LDYNGVMGVP ITFLHKFNPE QFELIKFRKG
310 320
VDEKDLSING KCPYFRILIK NKRLQK
Length:326
Mass (Da):38,044
Last modified:January 23, 2007 - v2
Checksum:i5F6AFE1DD1CBB1A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01675 Genomic DNA Translation: AAA26372.1
PIRiA92308 XYECP4
RefSeqiWP_002670781.1, NZ_NWAR01000096.1

Genome annotation databases

PATRICifig|562.8010.peg.1143

Similar proteinsi

Entry informationi

Entry nameiMTE1_ECOLX
AccessioniPrimary (citable) accession number: P00472
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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