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Protein

Thymidylate synthase

Gene

TD

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPBy similarity1
Active sitei156NucleophileBy similarity1
Binding sitei1795,10-methylenetetrahydrofolateBy similarity1
Binding sitei187dUMPBy similarity1
Binding sitei2855,10-methylenetetrahydrofolate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 137dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi176 – 179dUMPBy similarity4
Nucleotide bindingi217 – 219dUMPBy similarity3

GO - Molecular functioni

  • thymidylate synthase activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

SABIO-RKP00471.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TD
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410611 – 286Thymidylate synthaseAdd BLAST286

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Beta strandi26 – 30Combined sources5
Beta strandi33 – 37Combined sources5
Helixi38 – 40Combined sources3
Helixi53 – 63Combined sources11
Helixi69 – 77Combined sources9
Beta strandi80 – 83Combined sources4
Helixi88 – 91Combined sources4
Turni92 – 95Combined sources4
Helixi96 – 98Combined sources3
Helixi109 – 114Combined sources6
Helixi121 – 131Combined sources11
Helixi145 – 150Combined sources6
Beta strandi156 – 164Combined sources9
Beta strandi166 – 179Combined sources14
Turni180 – 183Combined sources4
Helixi184 – 202Combined sources19
Beta strandi205 – 221Combined sources17
Turni224 – 226Combined sources3
Helixi227 – 229Combined sources3
Helixi247 – 251Combined sources5
Helixi254 – 262Combined sources9
Helixi266 – 268Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TISX-ray2.70A1-286[»]
ProteinModelPortaliP00471.
SMRiP00471.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00471.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Phylogenomic databases

KOiK00560.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL
60 70 80 90 100
AWKACIAELI WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY
110 120 130 140 150
HSGELGPIYG KQWRDFGGVD QIIEVIDRIK KLPNDRRQIV SAWNPAELKY
160 170 180 190 200
MALPPCHMFY QFNVRNGYLD LQWYQRSVDV FLGLPFNIAS YATLVHIVAK
210 220 230 240 250
MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL VISGLPYKFR
260 270 280
YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV
Length:286
Mass (Da):33,073
Last modified:July 21, 1986 - v1
Checksum:i9F60863145F5219D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.
PIRiA00550. SYBPT4.
RefSeqiNP_049848.1. NC_000866.4.

Genome annotation databases

GeneIDi1258770.
KEGGivg:1258770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.
PIRiA00550. SYBPT4.
RefSeqiNP_049848.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TISX-ray2.70A1-286[»]
ProteinModelPortaliP00471.
SMRiP00471.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258770.
KEGGivg:1258770.

Phylogenomic databases

KOiK00560.

Enzyme and pathway databases

UniPathwayiUPA00575.
SABIO-RKP00471.

Miscellaneous databases

EvolutionaryTraceiP00471.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_BPT4
AccessioniPrimary (citable) accession number: P00471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is also expressed by the thyA gene of E.coli; the phage and host synthases exhibit striking dissimilarities in both structure and function.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.