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P00471

- TYSY_BPT4

UniProt

P00471 - TYSY_BPT4

Protein

Thymidylate synthase

Gene

TD

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei156 – 15611 Publication

    GO - Molecular functioni

    1. thymidylate synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. dTTP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate synthase (EC:2.1.1.45)
    Short name:
    TS
    Short name:
    TSase
    Gene namesi
    Name:TD
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 286286Thymidylate synthasePRO_0000141061Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    286
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1210
    Beta strandi26 – 305
    Beta strandi33 – 375
    Helixi38 – 403
    Helixi53 – 6311
    Helixi69 – 779
    Beta strandi80 – 834
    Helixi88 – 914
    Turni92 – 954
    Helixi96 – 983
    Helixi109 – 1146
    Helixi121 – 13111
    Helixi145 – 1506
    Beta strandi156 – 1649
    Beta strandi166 – 17914
    Turni180 – 1834
    Helixi184 – 20219
    Beta strandi205 – 22117
    Turni224 – 2263
    Helixi227 – 2293
    Helixi247 – 2515
    Helixi254 – 2629
    Helixi266 – 2683

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1TISX-ray2.70A1-286[»]
    ProteinModelPortaliP00471.
    SMRiP00471. Positions 1-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00471.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate synthase family.Curated

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00471-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL    50
    AWKACIAELI WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY 100
    HSGELGPIYG KQWRDFGGVD QIIEVIDRIK KLPNDRRQIV SAWNPAELKY 150
    MALPPCHMFY QFNVRNGYLD LQWYQRSVDV FLGLPFNIAS YATLVHIVAK 200
    MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL VISGLPYKFR 250
    YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV 286
    Length:286
    Mass (Da):33,073
    Last modified:July 21, 1986 - v1
    Checksum:i9F60863145F5219D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02035, K01804 Genomic DNA. Translation: AAA32492.1.
    AF158101 Genomic DNA. Translation: AAD42662.1.
    M12742 Genomic DNA. Translation: AAC12816.1.
    J03968 Genomic DNA. Translation: AAA32525.1.
    PIRiA00550. SYBPT4.
    RefSeqiNP_049848.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02035 , K01804 Genomic DNA. Translation: AAA32492.1 .
    AF158101 Genomic DNA. Translation: AAD42662.1 .
    M12742 Genomic DNA. Translation: AAC12816.1 .
    J03968 Genomic DNA. Translation: AAA32525.1 .
    PIRi A00550. SYBPT4.
    RefSeqi NP_049848.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1TIS X-ray 2.70 A 1-286 [» ]
    ProteinModelPortali P00471.
    SMRi P00471. Positions 1-286.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258770.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .

    Miscellaneous databases

    EvolutionaryTracei P00471.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Intervening sequence in the thymidylate synthase gene of bacteriophage T4."
      Chu F.K., Maley G.F., Maley F., Belfort M.
      Proc. Natl. Acad. Sci. U.S.A. 81:3049-3053(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase."
      Purohit S., Mathews C.K.
      J. Biol. Chem. 259:6261-6266(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
    4. "Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript."
      Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.
      Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-188.
    5. "Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase."
      Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.
      J. Biol. Chem. 263:16242-16251(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-286.
    6. "Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex."
      Finer-Moore J.S., Maley G.F., Maley F., Montfort W.R., Stroud R.M.
      Biochemistry 33:15459-15468(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

    Entry informationi

    Entry nameiTYSY_BPT4
    AccessioniPrimary (citable) accession number: P00471
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This enzyme is also expressed by the thyA gene of E.coli; the phage and host synthases exhibit striking dissimilarities in both structure and function.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3