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P00471 (TYSY_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:TD
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Subunit structure

Homodimer.

Miscellaneous

This enzyme is also expressed by the thyA gene of E.coli; the phage and host synthases exhibit striking dissimilarities in both structure and function.

Sequence similarities

Belongs to the thymidylate synthase family.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Thymidylate synthase
PRO_0000141061

Sites

Active site1561 Ref.1

Secondary structure

......................................... 286
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00471 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 9F60863145F5219D

FASTA28633,073
        10         20         30         40         50         60 
MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL AWKACIAELI 

        70         80         90        100        110        120 
WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY HSGELGPIYG KQWRDFGGVD 

       130        140        150        160        170        180 
QIIEVIDRIK KLPNDRRQIV SAWNPAELKY MALPPCHMFY QFNVRNGYLD LQWYQRSVDV 

       190        200        210        220        230        240 
FLGLPFNIAS YATLVHIVAK MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL 

       250        260        270        280 
VISGLPYKFR YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV 

« Hide

References

« Hide 'large scale' references
[1]"Intervening sequence in the thymidylate synthase gene of bacteriophage T4."
Chu F.K., Maley G.F., Maley F., Belfort M.
Proc. Natl. Acad. Sci. U.S.A. 81:3049-3053(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase."
Purohit S., Mathews C.K.
J. Biol. Chem. 259:6261-6266(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[4]"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript."
Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.
Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-188.
[5]"Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase."
Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.
J. Biol. Chem. 263:16242-16251(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-286.
[6]"Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex."
Finer-Moore J.S., Maley G.F., Maley F., Montfort W.R., Stroud R.M.
Biochemistry 33:15459-15468(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.
PIRSYBPT4. A00550.
RefSeqNP_049848.1. NC_000866.4.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TISX-ray2.70A1-286[»]
ProteinModelPortalP00471.
SMRP00471. Positions 1-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258770.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00471.

Entry information

Entry nameTYSY_BPT4
AccessionPrimary (citable) accession number: P00471
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways