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Protein

Thymidylate synthase

Gene

TD

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei156 – 15611 Publication

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

SABIO-RKP00471.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
Name:TD
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 286286Thymidylate synthasePRO_0000141061Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
286
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1210Combined sources
Beta strandi26 – 305Combined sources
Beta strandi33 – 375Combined sources
Helixi38 – 403Combined sources
Helixi53 – 6311Combined sources
Helixi69 – 779Combined sources
Beta strandi80 – 834Combined sources
Helixi88 – 914Combined sources
Turni92 – 954Combined sources
Helixi96 – 983Combined sources
Helixi109 – 1146Combined sources
Helixi121 – 13111Combined sources
Helixi145 – 1506Combined sources
Beta strandi156 – 1649Combined sources
Beta strandi166 – 17914Combined sources
Turni180 – 1834Combined sources
Helixi184 – 20219Combined sources
Beta strandi205 – 22117Combined sources
Turni224 – 2263Combined sources
Helixi227 – 2293Combined sources
Helixi247 – 2515Combined sources
Helixi254 – 2629Combined sources
Helixi266 – 2683Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TISX-ray2.70A1-286[»]
ProteinModelPortaliP00471.
SMRiP00471. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00471.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family.Curated

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00471-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL
60 70 80 90 100
AWKACIAELI WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY
110 120 130 140 150
HSGELGPIYG KQWRDFGGVD QIIEVIDRIK KLPNDRRQIV SAWNPAELKY
160 170 180 190 200
MALPPCHMFY QFNVRNGYLD LQWYQRSVDV FLGLPFNIAS YATLVHIVAK
210 220 230 240 250
MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL VISGLPYKFR
260 270 280
YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV
Length:286
Mass (Da):33,073
Last modified:July 21, 1986 - v1
Checksum:i9F60863145F5219D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.
PIRiA00550. SYBPT4.
RefSeqiNP_049848.1. NC_000866.4.

Genome annotation databases

GeneIDi1258770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.
PIRiA00550. SYBPT4.
RefSeqiNP_049848.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TISX-ray2.70A1-286[»]
ProteinModelPortaliP00471.
SMRiP00471. Positions 1-286.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258770.

Enzyme and pathway databases

UniPathwayiUPA00575.
SABIO-RKP00471.

Miscellaneous databases

EvolutionaryTraceiP00471.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Intervening sequence in the thymidylate synthase gene of bacteriophage T4."
    Chu F.K., Maley G.F., Maley F., Belfort M.
    Proc. Natl. Acad. Sci. U.S.A. 81:3049-3053(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase."
    Purohit S., Mathews C.K.
    J. Biol. Chem. 259:6261-6266(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
  4. "Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript."
    Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.
    Cell 45:157-166(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-188.
  5. "Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase."
    Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.
    J. Biol. Chem. 263:16242-16251(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-286.
  6. "Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex."
    Finer-Moore J.S., Maley G.F., Maley F., Montfort W.R., Stroud R.M.
    Biochemistry 33:15459-15468(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Entry informationi

Entry nameiTYSY_BPT4
AccessioniPrimary (citable) accession number: P00471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 7, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is also expressed by the thyA gene of E.coli; the phage and host synthases exhibit striking dissimilarities in both structure and function.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.