Reviewed, UniProtKB/Swiss-Prot P00471 (TYSY_BPT4)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TSase
      Short name=TS
    EC=2.1.1.45

Gene names

Name:

TD

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Subunit structure	Homodimer.
Miscellaneous	This enzyme is also expressed by the thyA gene of E.coli; the phage and host synthases exhibit striking dissimilarities in both structure and function.
Sequence similarities	Belongs to the thymidylate synthase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 286

286

Thymidylate synthase

PRO_0000141061

Sites

Active site

156

1

Ref.1

Secondary structure

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286

Helix Strand Turn

Details...

Helix

3 – 12

10

Beta strand

26 – 30

5

Beta strand

33 – 37

5

Helix

38 – 40

3

Helix

53 – 63

11

Helix

69 – 77

9

Beta strand

80 – 83

4

Helix

88 – 91

4

Turn

92 – 95

4

Helix

96 – 98

3

Helix

109 – 114

6

Helix

121 – 131

11

Helix

145 – 150

6

Beta strand

156 – 164

9

Beta strand

166 – 179

14

Turn

180 – 183

4

Helix

184 – 202

19

Beta strand

205 – 221

17

Turn

224 – 226

3

Helix

227 – 229

3

Helix

247 – 251

5

Helix

254 – 262

9

Helix

266 – 268

3

Sequences

	Sequence		Length	Mass (Da)
	P00471-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9F60863145F5219D	FASTA	286	33,073
	10 20 30 40 50 60 MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL AWKACIAELI 70 80 90 100 110 120 WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY HSGELGPIYG KQWRDFGGVD 130 140 150 160 170 180 QIIEVIDRIK KLPNDRRQIV SAWNPAELKY MALPPCHMFY QFNVRNGYLD LQWYQRSVDV 190 200 210 220 230 240 FLGLPFNIAS YATLVHIVAK MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL 250 260 270 280 VISGLPYKFR YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Intervening sequence in the thymidylate synthase gene of bacteriophage T4." Chu F.K., Maley G.F., Maley F., Belfort M. Proc. Natl. Acad. Sci. U.S.A. 81:3049-3053(1984) [PubMed: 6328492] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase." Purohit S., Mathews C.K. J. Biol. Chem. 259:6261-6266(1984) [PubMed: 6327673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
[4]	"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript." Chu F.K., Maley G.F., West D.K., Belfort M., Maley F. Cell 45:157-166(1986) [PubMed: 3698096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-188.
[5]	"Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase." Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R. J. Biol. Chem. 263:16242-16251(1988) [PubMed: 2846540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-286.
[6]	"Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex." Finer-Moore J.S., Maley G.F., Maley F., Montfort W.R., Stroud R.M. Biochemistry 33:15459-15468(1994) [PubMed: 7803410] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02035, K01804 Genomic DNA. Translation: AAA32492.1.
AF158101 Genomic DNA. Translation: AAD42662.1.
M12742 Genomic DNA. Translation: AAC12816.1.
J03968 Genomic DNA. Translation: AAA32525.1.

PIR

SYBPT4. A00550.

RefSeq

NP_049848.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TIS	X-ray	2.70	A	1-286	[»]

ModBase

Search...

Genome annotation databases

GeneID

1258770.

GenomeReviews

Gene locus T4p238 in contig AF158101_GR.

Enzyme and pathway databases

BRENDA

2.1.1.45. 1108.

Family and domain databases

InterPro

IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]

Gene3D

G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.

PANTHER

PTHR11549:SF2. Thymidylat_synth_C. 1 hit.

Pfam

PF00303. Thymidylat_synt. 1 hit.
[Graphical view]

PRINTS

PR00108. THYMDSNTHASE.

TIGRFAMs

TIGR03284. thym_sym. 1 hit.

PROSITE

PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TYSY_BPT4

Accession

Primary (citable) accession number: P00471

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)