P00456 (NIFH1_CLOPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogenase iron protein 1
EC=1.18.6.1

Alternative name(s):
Nitrogenase Fe protein 1
Nitrogenase component II
Nitrogenase reductase

Gene names

Name:

nifH1

Organism

Clostridium pasteurianum

Taxonomic identifier

1501 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein. HAMAP-Rule MF_00533
Catalytic activity	8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate. HAMAP-Rule MF_00533
Cofactor	Binds 1 4Fe-4S cluster per dimer.
Subunit structure	Homodimer.
Post-translational modification	The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity By similarity. HAMAP-Rule MF_00533
Sequence similarities	Belongs to the NifH/BchL/ChlL family.

Ontologies

Keywords
Biological process	Nitrogen fixation
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	ADP-ribosylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Cellular_component	molybdenum-iron nitrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: HAMAP carbonyl sulfide nitrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 273

273

Nitrogenase iron protein 1 HAMAP-Rule MF_00533

PRO_0000139498

Regions

Nucleotide binding

8 – 15

8

ATP Potential

Sites

Metal binding

94

1

Iron-sulfur (4Fe-4S); shared with dimeric partner

Metal binding

129

1

Iron-sulfur (4Fe-4S); shared with dimeric partner

Amino acid modifications

Modified residue

97

1

ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase HAMAP-Rule MF_00533

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

273

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Helix

14 – 26

13

Turn

27 – 29

3

Beta strand

32 – 37

6

Helix

45 – 48

4

Helix

56 – 63

8

Helix

64 – 66

3

Helix

69 – 72

4

Helix

77 – 79

3

Beta strand

81 – 84

4

Helix

95 – 108

14

Beta strand

117 – 124

8

Turn

130 – 133

4

Helix

134 – 137

4

Beta strand

143 – 148

6

Helix

152 – 168

17

Beta strand

175 – 182

8

Beta strand

185 – 187

3

Helix

190 – 200

11

Beta strand

204 – 208

5

Helix

212 – 219

8

Helix

224 – 227

4

Helix

232 – 246

15

Helix

258 – 268

11

Sequences

	Sequence		Length	Mass (Da)
	P00456 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A4EC06C5F58F08EC	FASTA	273	29,662
	10 20 30 40 50 60 MRQVAIYGKG GIGKSTTTQN LTSGLHAMGK TIMVVGCDPK ADSTRLLLGG LAQKSVLDTL 70 80 90 100 110 120 REEGEDVELD SILKEGYGGI RCVESGGPEP GVGCAGRGII TSINMLEQLG AYTDDLDYVF 130 140 150 160 170 180 YDVLGDVVCG GFAMPIREGK AQEIYIVASG EMMALYAANN ISKGIQKYAK SGGVRLGGII 190 200 210 220 230 240 CNSRKVANEY ELLDAFAKEL GSQLIHFVPR SPMVTKAEIN KQTVIEYDPT CEQAEEYREL 250 260 270 ARKVDANELF VIPKPMTQER LEEILMQYGL MDL

References

[1]	"The presence of five nifH-like sequences in Clostridium pasteurianum: sequence divergence and transcription properties." Wang S.-Z., Chen J.-S., Johnson J.L. Nucleic Acids Res. 16:439-454(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structural features of multiple nifH-like sequences and very biased codon usage in nitrogenase genes of Clostridium pasteurianum." Chen K.C.K., Chen J.-S., Johnson J.L. J. Bacteriol. 166:162-172(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The amino acid sequence of Clostridium pasteurianum iron protein, a component of nitrogenase. III. The NH2-terminal and COOH-terminal sequences, tryptic peptides of large cyanogen bromide peptides, and the complete sequence." Tanaka M., Haniu M., Yasunobu K.T., Mortenson L.E. J. Biol. Chem. 252:7093-7100(1977) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[4]	"Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum." Schlessman J.L., Woo D., Joshua-Tor L., Howard J.B., Rees D.C. J. Mol. Biol. 280:669-685(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.93 ANGSTROMS). Strain: ATCC 6013 / DSM 525 / NCIB 9486 / VKM B-1774 / W5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X07472 Genomic DNA. Translation: CAA30359.1.
AY603957 Genomic DNA. Translation: AAT37644.1.

PIR

NICLFP. A00533.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CP2	X-ray	1.93	A/B	1-269	[»]

ProteinModelPortal

P00456.

SMR

P00456. Positions 1-269.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:NIFH1CP-MONOMER.

Family and domain databases

HAMAP

MF_00533. NifH.

InterPro

IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]

Pfam

PF00142. Fer4_NifH. 1 hit.
[Graphical view]

PIRSF

PIRSF000363. Nitrogenase_iron. 1 hit.

PRINTS

PR00091. NITROGNASEII.

SUPFAM

SSF52540. SSF52540. 1 hit.

TIGRFAMs

TIGR01287. nifH. 1 hit.

PROSITE

PS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P00456.

Entry information

Entry name

NIFH1_CLOPA

Accession

Primary (citable) accession number: P00456

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 29, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program