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Protein

Nitrogenase iron protein 1

Gene

nifH1

Organism
Clostridium pasteurianum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

[4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster per dimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron-sulfur (4Fe-4S); shared with dimeric partner1
Metal bindingi129Iron-sulfur (4Fe-4S); shared with dimeric partner1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:NIFH1CP-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron protein 1 (EC:1.18.6.1)
Alternative name(s):
Nitrogenase Fe protein 1
Nitrogenase component II
Nitrogenase reductase
Gene namesi
Name:nifH1
OrganismiClostridium pasteurianum
Taxonomic identifieri1501 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001394981 – 273Nitrogenase iron protein 1Add BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseBy similarity1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.By similarity

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 8Combined sources7
Helixi14 – 26Combined sources13
Turni27 – 29Combined sources3
Beta strandi32 – 37Combined sources6
Helixi45 – 48Combined sources4
Helixi56 – 63Combined sources8
Helixi64 – 66Combined sources3
Helixi69 – 72Combined sources4
Helixi77 – 79Combined sources3
Beta strandi81 – 84Combined sources4
Helixi95 – 108Combined sources14
Beta strandi117 – 124Combined sources8
Turni130 – 133Combined sources4
Helixi134 – 137Combined sources4
Beta strandi143 – 148Combined sources6
Helixi152 – 168Combined sources17
Beta strandi175 – 182Combined sources8
Beta strandi185 – 187Combined sources3
Helixi190 – 200Combined sources11
Beta strandi204 – 208Combined sources5
Helixi212 – 219Combined sources8
Helixi224 – 227Combined sources4
Helixi232 – 246Combined sources15
Helixi258 – 268Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CP2X-ray1.93A/B1-269[»]
ProteinModelPortaliP00456.
SMRiP00456.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00456.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.Curated

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQVAIYGKG GIGKSTTTQN LTSGLHAMGK TIMVVGCDPK ADSTRLLLGG
60 70 80 90 100
LAQKSVLDTL REEGEDVELD SILKEGYGGI RCVESGGPEP GVGCAGRGII
110 120 130 140 150
TSINMLEQLG AYTDDLDYVF YDVLGDVVCG GFAMPIREGK AQEIYIVASG
160 170 180 190 200
EMMALYAANN ISKGIQKYAK SGGVRLGGII CNSRKVANEY ELLDAFAKEL
210 220 230 240 250
GSQLIHFVPR SPMVTKAEIN KQTVIEYDPT CEQAEEYREL ARKVDANELF
260 270
VIPKPMTQER LEEILMQYGL MDL
Length:273
Mass (Da):29,662
Last modified:July 21, 1986 - v1
Checksum:iA4EC06C5F58F08EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07472 Genomic DNA. Translation: CAA30359.1.
AY603957 Genomic DNA. Translation: AAT37644.1.
PIRiA00533. NICLFP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07472 Genomic DNA. Translation: CAA30359.1.
AY603957 Genomic DNA. Translation: AAT37644.1.
PIRiA00533. NICLFP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CP2X-ray1.93A/B1-269[»]
ProteinModelPortaliP00456.
SMRiP00456.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:NIFH1CP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP00456.

Family and domain databases

CDDicd02040. NifH. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00533. NifH. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR005977. Nitrogenase_Fe_NifH.
IPR027417. P-loop_NTPase.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01287. nifH. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFH1_CLOPA
AccessioniPrimary (citable) accession number: P00456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.