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P00448 (SODM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Superoxide dismutase [Mn]

EC=1.15.1.1
Alternative name(s):
MnSOD
Gene names
Name:sodA
Ordered Locus Names:b3908, JW3879
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length206 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Catalytic activity

2 superoxide + 2 H+ = O2 + H2O2.

Cofactor

Binds 1 manganese ion per subunit.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the iron/manganese superoxide dismutase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5 Ref.9 Ref.10
Chain2 – 206205Superoxide dismutase [Mn]
PRO_0000160030

Sites

Metal binding271Manganese
Metal binding821Manganese
Metal binding1681Manganese
Metal binding1721Manganese

Natural variations

Natural variant1651M → L in strain: B.

Experimental info

Sequence conflict81 – 822NH → HN Ref.6

Secondary structure

...................................... 206
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P00448 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 082DAF56EA3C15CC

FASTA20623,097
        10         20         30         40         50         60 
MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK 

        70         80         90        100        110        120 
LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA 

       130        140        150        160        170        180 
AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN 

       190        200 
RRPDYIKEFW NVVNWDEAAA RFAAKK 

« Hide

References

« Hide 'large scale' references
[1]"Structure and gene expression of the E. coli Mn-superoxide dismutase gene."
Takeda Y., Avila H.
Nucleic Acids Res. 14:4577-4589(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The amino acid sequence of mangano superoxide dismutase from Escherichia coli B."
Steinman H.M.
J. Biol. Chem. 253:8708-8720(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-206.
Strain: B.
[6]"Nucleotide sequence of the rhaR-sodA interval specifying rhaT in Escherichia coli."
Garcia C., Baldoma L., Badia J., Aguilar J.
J. Gen. Microbiol. 138:1109-1116(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
Strain: K12.
[7]"Mapping, cloning, expression, and sequencing of the rhaT gene, which encodes a novel L-rhamnose-H+ transport protein in Salmonella typhimurium and Escherichia coli."
Tate C.G., Muiry J.A.R., Henderson P.J.F.
J. Biol. Chem. 267:6923-6932(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
Strain: K12.
[8]"Transcriptional and posttranscriptional regulation of manganese superoxide dismutase biosynthesis in Escherichia coli, studied with operon and protein fusions."
Touati D.
J. Bacteriol. 170:2511-2520(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2.
[9]Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F.
Submitted (SEP-1994) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-11.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[10]"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
Link A.J., Robison K., Church G.M.
Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13.
Strain: K12 / EMG2.
[11]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[12]"Outer sphere mutations perturb metal reactivity in manganese superoxide dismutase."
Edwards R.A., Whittaker M.M., Whittaker J.W., Baker E.N., Jameson G.B.
Biochemistry 40:15-27(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF MUTANTS PHE-35; LEU-147 AND HIS-147.
[13]"Removing a hydrogen bond in the dimer interface of Escherichia coli manganese superoxide dismutase alters structure and reactivity."
Edwards R.A., Whittaker M.M., Whittaker J.W., Baker E.N., Jameson G.B.
Biochemistry 40:4622-4632(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF MUTANTS ALA-31 AND PHE-175.
[14]"Paramagnetic NMR spectroscopy of native and cobalt substituted manganese superoxide dismutase from Escherichia coli."
Renault J.P., Verchere-Beau R., Morgenstern-Badarau I., Piccioli M.
FEBS Lett. 401:15-19(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
[15]"Crystal structure of Escherichia coli manganese superoxide dismutase at 2.1-A resolution."
Edwards R.A., Baker H.M., Whittaker M.M., Whittaker J.W., Jameson G.B., Baker E.N.
J. Biol. Inorg. Chem. 3:161-171(1998)
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[16]"Cryo-trapping the six-coordinate, distorted-octahedral active site of manganese superoxide dismutase."
Borgstahl G.E., Pokross M., Chehab R., Sekher A., Snell E.H.
J. Mol. Biol. 296:951-959(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03951 Genomic DNA. Translation: CAA27580.1.
M20984 Genomic DNA. No translation available.
L19201 Genomic DNA. Translation: AAB03041.1.
U00096 Genomic DNA. Translation: AAC76890.1.
AP009048 Genomic DNA. Translation: BAE77401.1.
X60699 Genomic DNA. Translation: CAA43108.1.
M85158 Genomic DNA. Translation: AAA24528.1. Sequence problems.
PIRDSECN. A24141.
RefSeqNP_418344.3. NC_000913.3.
YP_491542.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D5NX-ray1.55A/B/C/D2-206[»]
1EN4X-ray2.00A/B/C/D2-206[»]
1EN5X-ray2.30A/B/C/D2-206[»]
1EN6X-ray2.00A/B/C/D2-206[»]
1I08X-ray2.20A/B/C/D2-206[»]
1I0HX-ray1.35A/B2-206[»]
1IX9X-ray0.90A/B2-206[»]
1IXBX-ray0.90A/B2-206[»]
1MMMX-ray2.20A/B2-206[»]
1VEWX-ray2.10A/B/C/D2-206[»]
1ZLZX-ray1.55A/B2-206[»]
3K9SX-ray1.55A/B/C/D2-206[»]
3OT7X-ray1.90A/B/C/D2-206[»]
ProteinModelPortalP00448.
SMRP00448. Positions 2-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP00448. 7 interactions.
STRING511145.b3908.

2D gel databases

SWISS-2DPAGEP00448.

Proteomic databases

PaxDbP00448.
PRIDEP00448.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76890; AAC76890; b3908.
BAE77401; BAE77401; BAE77401.
GeneID12933233.
948403.
KEGGecj:Y75_p3278.
eco:b3908.
PATRIC32123327. VBIEscCol129921_4024.

Organism-specific databases

EchoBASEEB0946.
EcoGeneEG10953. sodA.

Phylogenomic databases

eggNOGCOG0605.
HOGENOMHOG000013583.
KOK04564.
OMADYISAFW.
OrthoDBEOG63NMNT.
PhylomeDBP00448.
ProtClustDBPRK10925.

Enzyme and pathway databases

BioCycEcoCyc:SUPEROX-DISMUTMN-MONOMER.
ECOL316407:JW3879-MONOMER.
MetaCyc:SUPEROX-DISMUTMN-MONOMER.

Gene expression databases

GenevestigatorP00448.

Family and domain databases

InterProIPR001189. Mn/Fe_SOD.
IPR019833. Mn/Fe_SOD_BS.
IPR019832. Mn/Fe_SOD_C.
IPR019831. Mn/Fe_SOD_N.
[Graphical view]
PANTHERPTHR11404. PTHR11404. 1 hit.
PfamPF02777. Sod_Fe_C. 1 hit.
PF00081. Sod_Fe_N. 1 hit.
[Graphical view]
PIRSFPIRSF000349. SODismutase. 1 hit.
PRINTSPR01703. MNSODISMTASE.
SUPFAMSSF46609. SSF46609. 1 hit.
SSF54719. SSF54719. 1 hit.
PROSITEPS00088. SOD_MN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP00448.
PROP00448.

Entry information

Entry nameSODM_ECOLI
AccessionPrimary (citable) accession number: P00448
Secondary accession number(s): Q2M8K5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene