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Protein

Superoxide dismutase [Mn]

Gene

sodA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Catalytic activityi

2 superoxide + 2 H+ = O2 + H2O2.

Cofactori

Mn2+Note: Binds 1 Mn2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Manganese1
Metal bindingi82Manganese1
Metal bindingi168Manganese1
Metal bindingi172Manganese1

GO - Molecular functioni

  • antioxidant activity Source: EcoCyc
  • DNA binding Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • metal ion binding Source: EcoliWiki
  • superoxide dismutase activity Source: EcoCyc

GO - Biological processi

  • cellular response to selenium ion Source: EcoCyc
  • oxidation-reduction process Source: EcoliWiki
  • removal of superoxide radicals Source: EcoliWiki
  • response to acidic pH Source: EcoCyc
  • response to heat Source: EcoCyc
  • response to oxidative stress Source: EcoCyc
  • superoxide metabolic process Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:SUPEROX-DISMUTMN-MONOMER
MetaCyc:SUPEROX-DISMUTMN-MONOMER
BRENDAi1.15.1.1 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide dismutase [Mn] (EC:1.15.1.1)
Alternative name(s):
MnSOD
Gene namesi
Name:sodA
Ordered Locus Names:b3908, JW3879
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10953 sodA

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved3 Publications
ChainiPRO_00001600302 – 206Superoxide dismutase [Mn]Add BLAST205

Proteomic databases

EPDiP00448
PaxDbiP00448
PRIDEiP00448

2D gel databases

SWISS-2DPAGEiP00448

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4262227, 66 interactors
IntActiP00448, 7 interactors
STRINGi316385.ECDH10B_4098

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 18Combined sources7
Helixi21 – 29Combined sources9
Helixi31 – 43Combined sources13
Helixi47 – 50Combined sources4
Helixi54 – 57Combined sources4
Helixi61 – 63Combined sources3
Helixi66 – 68Combined sources3
Helixi69 – 87Combined sources19
Helixi97 – 107Combined sources11
Helixi110 – 123Combined sources14
Beta strandi126 – 135Combined sources10
Beta strandi138 – 145Combined sources8
Helixi150 – 152Combined sources3
Helixi154 – 157Combined sources4
Beta strandi161 – 168Combined sources8
Helixi171 – 173Combined sources3
Helixi175 – 178Combined sources4
Helixi182 – 192Combined sources11
Helixi195 – 205Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5NX-ray1.55A/B/C/D2-206[»]
1EN4X-ray2.00A/B/C/D2-206[»]
1EN5X-ray2.30A/B/C/D2-206[»]
1EN6X-ray2.00A/B/C/D2-206[»]
1I08X-ray2.20A/B/C/D2-206[»]
1I0HX-ray1.35A/B2-206[»]
1IX9X-ray0.90A/B2-206[»]
1IXBX-ray0.90A/B2-206[»]
1MMMX-ray2.20A/B2-206[»]
1VEWX-ray2.10A/B/C/D2-206[»]
1ZLZX-ray1.55A/B2-206[»]
3K9SX-ray1.55A/B/C/D2-206[»]
3OT7X-ray1.90A/B/C/D2-206[»]
ProteinModelPortaliP00448
SMRiP00448
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00448

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CK4 Bacteria
COG0605 LUCA
HOGENOMiHOG000013583
InParanoidiP00448
KOiK04564
OMAiYEGWKGE
PhylomeDBiP00448

Family and domain databases

Gene3Di1.10.287.990, 1 hit
2.40.500.20, 1 hit
InterProiView protein in InterPro
IPR001189 Mn/Fe_SOD
IPR019833 Mn/Fe_SOD_BS
IPR019832 Mn/Fe_SOD_C
IPR019831 Mn/Fe_SOD_N
IPR036324 Mn/Fe_SOD_N_sf
IPR036314 SOD_C_sf
PfamiView protein in Pfam
PF02777 Sod_Fe_C, 1 hit
PF00081 Sod_Fe_N, 1 hit
PIRSFiPIRSF000349 SODismutase, 1 hit
PRINTSiPR01703 MNSODISMTASE
SUPFAMiSSF46609 SSF46609, 1 hit
SSF54719 SSF54719, 1 hit
PROSITEiView protein in PROSITE
PS00088 SOD_MN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00448-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA
60 70 80 90 100
NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK
110 120 130 140 150
AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP
160 170 180 190 200
LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA

RFAAKK
Length:206
Mass (Da):23,097
Last modified:January 23, 2007 - v2
Checksum:i082DAF56EA3C15CC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti81 – 82NH → HN (PubMed:1339463).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti165M → L in strain: B. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03951 Genomic DNA Translation: CAA27580.1
M20984 Genomic DNA No translation available.
L19201 Genomic DNA Translation: AAB03041.1
U00096 Genomic DNA Translation: AAC76890.1
AP009048 Genomic DNA Translation: BAE77401.1
X60699 Genomic DNA Translation: CAA43108.1
M85158 Genomic DNA Translation: AAA24528.1 Sequence problems.
PIRiA24141 DSECN
RefSeqiNP_418344.3, NC_000913.3
WP_000122641.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76890; AAC76890; b3908
BAE77401; BAE77401; BAE77401
GeneIDi948403
KEGGiecj:JW3879
eco:b3908
PATRICifig|1411691.4.peg.2797

Similar proteinsi

Entry informationi

Entry nameiSODM_ECOLI
AccessioniPrimary (citable) accession number: P00448
Secondary accession number(s): Q2M8K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health