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Protein

Tyrosinase

Gene

T

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activityi

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
L-tyrosine + O2 = dopaquinone + H2O.

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Copper ABy similarity
Metal bindingi97 – 971Copper ABy similarity
Metal bindingi106 – 1061Copper ABy similarity
Metal bindingi278 – 2781Copper BBy similarity
Metal bindingi282 – 2821Copper BBy similarity
Metal bindingi307 – 3071Copper BBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosinase (EC:1.14.18.1)
Alternative name(s):
Monophenol monooxygenase
Gene namesi
Name:T
ORF Names:90C4.150, NCU00776
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 1, Linkage Group I

Organism-specific databases

EuPathDBiFungiDB:NCU00776.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 408407TyrosinasePRO_0000035895Add
BLAST
Propeptidei409 – 685277Could be involved in enzyme activationPRO_0000035896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine
Cross-linki95 ↔ 972'-(S-cysteinyl)-histidine (Cys-His)

Keywords - PTMi

Acetylation, Thioether bond

Structurei

3D structure databases

ProteinModelPortaliP00440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Phylogenomic databases

eggNOGiNOG68512.
InParanoidiP00440.
OrthoDBiEOG79PJXV.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016216. Monophenol_mOase_fun.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000340. MPO_fungal. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDIKFAIT GVPTPPSSNG AVPLRRELRD LQQNYPEQFN LYLLGLRDFQ
60 70 80 90 100
GLDEAKLDSY YQVAGIHGMP FKPWAGVPSD TDWSQPGSSG FGGYCTHSSI
110 120 130 140 150
LFITWHRPYL ALYEQALYAS VQAVAQKFPV EGGLRAKYVA AAKDFRAPYF
160 170 180 190 200
DWASQPPKGT LAFPESLSSR TIQVVDVDGK TKSINNPLHR FTFHPVNPSP
210 220 230 240 250
GDFSAAWSRY PSTVRYPNRL TGASRDERIA PILANELASL RNNVSLLLLS
260 270 280 290 300
YKDFDAFSYN RWDPNTNPGD FGSLEDVHNE IHDRTGGNGH MSSLEVSAFD
310 320 330 340 350
PLFWLHHVNV DRLWSIWQDL NPNSFMTPRP APYSTFVAQE GESQSKSTPL
360 370 380 390 400
EPFWDKSAAN FWTSEQVKDS ITFGYAYPET QKWKYSSVKE YQAAIRKSVT
410 420 430 440 450
ALYGSNVFAN FVENVADRTP ALKKPQATGE ESKSTVSAAA AHAVELSGAK
460 470 480 490 500
KVAEKVHNVF QHAEEKAQKP VVPVKDTKAE SSTAAGMMIG LSIKRPSKLT
510 520 530 540 550
ASPGPIPESL KYLAPDGKYT DWIVNVRAQK HGLGQSFRVI VFLGEFNPDP
560 570 580 590 600
ETWDDEFNCV GRVSVLGRSA ETQCGKCRKD NANGLIVSGT VPLTSALLQD
610 620 630 640 650
IVGGELQSLK PEDVIPHLRA NLKWKVALFN GDEYNLEEVP DLKVSVASTE
660 670 680
VTIDEEGLPH YSRQYTVYPE ITEGKPCGHG PEDHI
Length:685
Mass (Da):75,886
Last modified:December 4, 2007 - v5
Checksum:iDF64B764BFF5468A
GO

Sequence cautioni

The sequence AAA33618.1 differs from that shown. Reason: Frameshift at position 596. Curated
The sequence AAA33619.1 differs from that shown. Reason: Frameshift at position 596. Curated
The sequence EAA35696.3 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti235 – 2351N → D AA sequence (PubMed:6210696).Curated
Sequence conflicti235 – 2351N → D AA sequence (PubMed:6210697).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti15 – 151P → T in strain: Sing, TL and TS.
Natural varianti30 – 301D → E in strain: Sing.
Natural varianti130 – 1301V → T in strain: Sing.
Natural varianti202 – 2021D → N in strain: TL.
Natural varianti346 – 3472KS → QN in strain: Sing.
Natural varianti371 – 3711I → T in strain: Sing.
Natural varianti424 – 4241K → N in strain: TS.
Natural varianti450 – 4501K → R in strain: TS.
Natural varianti678 – 6781G → R in strain: TS.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32843 Genomic DNA. Translation: AAA33619.1. Frameshift.
M33271 Genomic DNA. Translation: AAA33618.1. Frameshift.
BX842680 Genomic DNA. Translation: CAE81941.1.
CM002236 Genomic DNA. Translation: EAA35696.3. Sequence problems.
PIRiA34460. YRNC.
RefSeqiXP_964932.3. XM_959839.3.

Genome annotation databases

EnsemblFungiiEFNCRT00000000584; EFNCRP00000000584; EFNCRG00000000584.
GeneIDi3881081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32843 Genomic DNA. Translation: AAA33619.1. Frameshift.
M33271 Genomic DNA. Translation: AAA33618.1. Frameshift.
BX842680 Genomic DNA. Translation: CAE81941.1.
CM002236 Genomic DNA. Translation: EAA35696.3. Sequence problems.
PIRiA34460. YRNC.
RefSeqiXP_964932.3. XM_959839.3.

3D structure databases

ProteinModelPortaliP00440.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000000584; EFNCRP00000000584; EFNCRG00000000584.
GeneIDi3881081.

Organism-specific databases

EuPathDBiFungiDB:NCU00776.

Phylogenomic databases

eggNOGiNOG68512.
InParanoidiP00440.
OrthoDBiEOG79PJXV.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016216. Monophenol_mOase_fun.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000340. MPO_fungal. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the tyrosinase gene from Neurospora crassa."
    Kupper U., Niedermann D.M., Travaglini G., Lerch K.
    J. Biol. Chem. 264:17250-17258(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oak Ridge and TS.
  2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  4. "Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether."
    Lerch K.
    J. Biol. Chem. 257:6414-6419(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-408.
    Strain: TL.
  5. "Comparison of amino acid sequence and thermostability of tyrosinase from three wild type strains of Neurospora crassa."
    Ruegg C., Ammer D., Lerch K.
    J. Biol. Chem. 257:6420-6426(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-408.
    Strain: Sing and TS.

Entry informationi

Entry nameiTYRO_NEUCR
AccessioniPrimary (citable) accession number: P00440
Secondary accession number(s): Q6MGJ7, Q7RVL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 4, 2007
Last modified: July 22, 2015
This is version 107 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.