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P00440 (TYRO_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosinase

EC=1.14.18.1
Alternative name(s):
Monophenol monooxygenase
Gene names
Name:T
ORF Names:90C4.150, NCU00776
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length685 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

Catalytic activity

2 L-dopa + O2 = 2 dopaquinone + 2 H2O.

L-tyrosine + O2 = dopaquinone + H2O.

Cofactor

Binds 2 copper ions per subunit By similarity.

Sequence similarities

Belongs to the tyrosinase family.

Sequence caution

The sequence AAA33618.1 differs from that shown. Reason: Frameshift at position 596.

The sequence AAA33619.1 differs from that shown. Reason: Frameshift at position 596.

The sequence EAA35696.3 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4 Ref.5
Chain2 – 408407Tyrosinase
PRO_0000035895
Propeptide409 – 685277Could be involved in enzyme activation
PRO_0000035896

Sites

Metal binding671Copper A By similarity
Metal binding971Copper A By similarity
Metal binding1061Copper A By similarity
Metal binding2781Copper B By similarity
Metal binding2821Copper B By similarity
Metal binding3071Copper B By similarity

Amino acid modifications

Modified residue21N-acetylserine
Cross-link95 ↔ 972'-(S-cysteinyl)-histidine (Cys-His)

Natural variations

Natural variant151P → T in strain: Sing, TL and TS.
Natural variant301D → E in strain: Sing.
Natural variant1301V → T in strain: Sing.
Natural variant2021D → N in strain: TL.
Natural variant346 – 3472KS → QN in strain: Sing.
Natural variant3711I → T in strain: Sing.
Natural variant4241K → N in strain: TS.
Natural variant4501K → R in strain: TS.
Natural variant6781G → R in strain: TS.

Experimental info

Sequence conflict2351N → D AA sequence Ref.4
Sequence conflict2351N → D AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P00440 [UniParc].

Last modified December 4, 2007. Version 5.
Checksum: DF64B764BFF5468A

FASTA68575,886
        10         20         30         40         50         60 
MSTDIKFAIT GVPTPPSSNG AVPLRRELRD LQQNYPEQFN LYLLGLRDFQ GLDEAKLDSY 

        70         80         90        100        110        120 
YQVAGIHGMP FKPWAGVPSD TDWSQPGSSG FGGYCTHSSI LFITWHRPYL ALYEQALYAS 

       130        140        150        160        170        180 
VQAVAQKFPV EGGLRAKYVA AAKDFRAPYF DWASQPPKGT LAFPESLSSR TIQVVDVDGK 

       190        200        210        220        230        240 
TKSINNPLHR FTFHPVNPSP GDFSAAWSRY PSTVRYPNRL TGASRDERIA PILANELASL 

       250        260        270        280        290        300 
RNNVSLLLLS YKDFDAFSYN RWDPNTNPGD FGSLEDVHNE IHDRTGGNGH MSSLEVSAFD 

       310        320        330        340        350        360 
PLFWLHHVNV DRLWSIWQDL NPNSFMTPRP APYSTFVAQE GESQSKSTPL EPFWDKSAAN 

       370        380        390        400        410        420 
FWTSEQVKDS ITFGYAYPET QKWKYSSVKE YQAAIRKSVT ALYGSNVFAN FVENVADRTP 

       430        440        450        460        470        480 
ALKKPQATGE ESKSTVSAAA AHAVELSGAK KVAEKVHNVF QHAEEKAQKP VVPVKDTKAE 

       490        500        510        520        530        540 
SSTAAGMMIG LSIKRPSKLT ASPGPIPESL KYLAPDGKYT DWIVNVRAQK HGLGQSFRVI 

       550        560        570        580        590        600 
VFLGEFNPDP ETWDDEFNCV GRVSVLGRSA ETQCGKCRKD NANGLIVSGT VPLTSALLQD 

       610        620        630        640        650        660 
IVGGELQSLK PEDVIPHLRA NLKWKVALFN GDEYNLEEVP DLKVSVASTE VTIDEEGLPH 

       670        680 
YSRQYTVYPE ITEGKPCGHG PEDHI 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the tyrosinase gene from Neurospora crassa."
Kupper U., Niedermann D.M., Travaglini G., Lerch K.
J. Biol. Chem. 264:17250-17258(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Oak Ridge and TS.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether."
Lerch K.
J. Biol. Chem. 257:6414-6419(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-408.
Strain: TL.
[5]"Comparison of amino acid sequence and thermostability of tyrosinase from three wild type strains of Neurospora crassa."
Ruegg C., Ammer D., Lerch K.
J. Biol. Chem. 257:6420-6426(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-408.
Strain: Sing and TS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32843 Genomic DNA. Translation: AAA33619.1. Frameshift.
M33271 Genomic DNA. Translation: AAA33618.1. Frameshift.
BX842680 Genomic DNA. Translation: CAE81941.1.
CM002236 Genomic DNA. Translation: EAA35696.3. Sequence problems.
PIRYRNC. A34460.
RefSeqXP_964932.2. XM_959839.2.

3D structure databases

ProteinModelPortalP00440.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU00776.1.

Chemistry

DrugBankDB00548. Azelaic Acid.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000000584; EFNCRP00000000584; EFNCRG00000000584.
GeneID3881081.
KEGGncr:NCU00776.

Phylogenomic databases

eggNOGNOG68512.
KOK00505.
OrthoDBEOG79PJXV.

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
InterProIPR016216. Monophenol_mOase_fun.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamPF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFPIRSF000340. MPO_fungal. 1 hit.
PRINTSPR00092. TYROSINASE.
SUPFAMSSF48056. SSF48056. 1 hit.
PROSITEPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYRO_NEUCR
AccessionPrimary (citable) accession number: P00440
Secondary accession number(s): Q6MGJ7, Q7RVL7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 100 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families