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P00440

- TYRO_NEUCR

UniProt

P00440 - TYRO_NEUCR

Protein

Tyrosinase

Gene

T

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 5 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds.

    Catalytic activityi

    2 L-dopa + O2 = 2 dopaquinone + 2 H2O.
    L-tyrosine + O2 = dopaquinone + H2O.

    Cofactori

    Binds 2 copper ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi67 – 671Copper ABy similarity
    Metal bindingi97 – 971Copper ABy similarity
    Metal bindingi106 – 1061Copper ABy similarity
    Metal bindingi278 – 2781Copper BBy similarity
    Metal bindingi282 – 2821Copper BBy similarity
    Metal bindingi307 – 3071Copper BBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. monophenol monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosinase (EC:1.14.18.1)
    Alternative name(s):
    Monophenol monooxygenase
    Gene namesi
    Name:T
    ORF Names:90C4.150, NCU00776
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 1, Linkage Group I

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 408407TyrosinasePRO_0000035895Add
    BLAST
    Propeptidei409 – 685277Could be involved in enzyme activationPRO_0000035896Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine
    Cross-linki95 ↔ 972'-(S-cysteinyl)-histidine (Cys-His)

    Keywords - PTMi

    Acetylation, Thioether bond

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU00776.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP00440.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tyrosinase family.Curated

    Phylogenomic databases

    eggNOGiNOG68512.
    KOiK00505.
    OrthoDBiEOG79PJXV.

    Family and domain databases

    Gene3Di1.10.1280.10. 1 hit.
    InterProiIPR016216. Monophenol_mOase_fun.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view]
    PfamiPF00264. Tyrosinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000340. MPO_fungal. 1 hit.
    PRINTSiPR00092. TYROSINASE.
    SUPFAMiSSF48056. SSF48056. 1 hit.
    PROSITEiPS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00440-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTDIKFAIT GVPTPPSSNG AVPLRRELRD LQQNYPEQFN LYLLGLRDFQ    50
    GLDEAKLDSY YQVAGIHGMP FKPWAGVPSD TDWSQPGSSG FGGYCTHSSI 100
    LFITWHRPYL ALYEQALYAS VQAVAQKFPV EGGLRAKYVA AAKDFRAPYF 150
    DWASQPPKGT LAFPESLSSR TIQVVDVDGK TKSINNPLHR FTFHPVNPSP 200
    GDFSAAWSRY PSTVRYPNRL TGASRDERIA PILANELASL RNNVSLLLLS 250
    YKDFDAFSYN RWDPNTNPGD FGSLEDVHNE IHDRTGGNGH MSSLEVSAFD 300
    PLFWLHHVNV DRLWSIWQDL NPNSFMTPRP APYSTFVAQE GESQSKSTPL 350
    EPFWDKSAAN FWTSEQVKDS ITFGYAYPET QKWKYSSVKE YQAAIRKSVT 400
    ALYGSNVFAN FVENVADRTP ALKKPQATGE ESKSTVSAAA AHAVELSGAK 450
    KVAEKVHNVF QHAEEKAQKP VVPVKDTKAE SSTAAGMMIG LSIKRPSKLT 500
    ASPGPIPESL KYLAPDGKYT DWIVNVRAQK HGLGQSFRVI VFLGEFNPDP 550
    ETWDDEFNCV GRVSVLGRSA ETQCGKCRKD NANGLIVSGT VPLTSALLQD 600
    IVGGELQSLK PEDVIPHLRA NLKWKVALFN GDEYNLEEVP DLKVSVASTE 650
    VTIDEEGLPH YSRQYTVYPE ITEGKPCGHG PEDHI 685
    Length:685
    Mass (Da):75,886
    Last modified:December 4, 2007 - v5
    Checksum:iDF64B764BFF5468A
    GO

    Sequence cautioni

    The sequence AAA33618.1 differs from that shown. Reason: Frameshift at position 596.
    The sequence AAA33619.1 differs from that shown. Reason: Frameshift at position 596.
    The sequence EAA35696.3 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti235 – 2351N → D AA sequence (PubMed:6210696)Curated
    Sequence conflicti235 – 2351N → D AA sequence (PubMed:6210697)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti15 – 151P → T in strain: Sing, TL and TS.
    Natural varianti30 – 301D → E in strain: Sing.
    Natural varianti130 – 1301V → T in strain: Sing.
    Natural varianti202 – 2021D → N in strain: TL.
    Natural varianti346 – 3472KS → QN in strain: Sing.
    Natural varianti371 – 3711I → T in strain: Sing.
    Natural varianti424 – 4241K → N in strain: TS.
    Natural varianti450 – 4501K → R in strain: TS.
    Natural varianti678 – 6781G → R in strain: TS.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32843 Genomic DNA. Translation: AAA33619.1. Frameshift.
    M33271 Genomic DNA. Translation: AAA33618.1. Frameshift.
    BX842680 Genomic DNA. Translation: CAE81941.1.
    CM002236 Genomic DNA. Translation: EAA35696.3. Sequence problems.
    PIRiA34460. YRNC.
    RefSeqiXP_964932.2. XM_959839.2.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000000584; EFNCRP00000000584; EFNCRG00000000584.
    GeneIDi3881081.
    KEGGincr:NCU00776.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32843 Genomic DNA. Translation: AAA33619.1 . Frameshift.
    M33271 Genomic DNA. Translation: AAA33618.1 . Frameshift.
    BX842680 Genomic DNA. Translation: CAE81941.1 .
    CM002236 Genomic DNA. Translation: EAA35696.3 . Sequence problems.
    PIRi A34460. YRNC.
    RefSeqi XP_964932.2. XM_959839.2.

    3D structure databases

    ProteinModelPortali P00440.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU00776.1.

    Chemistry

    DrugBanki DB00548. Azelaic Acid.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000000584 ; EFNCRP00000000584 ; EFNCRG00000000584 .
    GeneIDi 3881081.
    KEGGi ncr:NCU00776.

    Phylogenomic databases

    eggNOGi NOG68512.
    KOi K00505.
    OrthoDBi EOG79PJXV.

    Family and domain databases

    Gene3Di 1.10.1280.10. 1 hit.
    InterProi IPR016216. Monophenol_mOase_fun.
    IPR002227. Tyrosinase_Cu-bd.
    IPR008922. Unchr_di-copper_centre.
    [Graphical view ]
    Pfami PF00264. Tyrosinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000340. MPO_fungal. 1 hit.
    PRINTSi PR00092. TYROSINASE.
    SUPFAMi SSF48056. SSF48056. 1 hit.
    PROSITEi PS00497. TYROSINASE_1. 1 hit.
    PS00498. TYROSINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the tyrosinase gene from Neurospora crassa."
      Kupper U., Niedermann D.M., Travaglini G., Lerch K.
      J. Biol. Chem. 264:17250-17258(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Oak Ridge and TS.
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    4. "Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether."
      Lerch K.
      J. Biol. Chem. 257:6414-6419(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-408.
      Strain: TL.
    5. "Comparison of amino acid sequence and thermostability of tyrosinase from three wild type strains of Neurospora crassa."
      Ruegg C., Ammer D., Lerch K.
      J. Biol. Chem. 257:6420-6426(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-408.
      Strain: Sing and TS.

    Entry informationi

    Entry nameiTYRO_NEUCR
    AccessioniPrimary (citable) accession number: P00440
    Secondary accession number(s): Q6MGJ7, Q7RVL7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3