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Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene

pcaH

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase beta chain (pcaH), Protocatechuate 3,4-dioxygenase alpha chain (pcaG)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109Iron; catalytic1 Publication1
Metal bindingi148Iron; catalytic1 Publication1
Metal bindingi161Iron; catalytic1 Publication1
Metal bindingi163Iron; catalytic1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3185.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaH
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000850982 – 239Protocatechuate 3,4-dioxygenase beta chainAdd BLAST238

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Protein-protein interaction databases

IntActiP00437. 1 interactor.
STRINGi160488.PP_4656.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Turni14 – 16Combined sources3
Helixi26 – 28Combined sources3
Turni29 – 31Combined sources3
Helixi44 – 47Combined sources4
Turni59 – 62Combined sources4
Turni64 – 68Combined sources5
Beta strandi70 – 72Combined sources3
Beta strandi78 – 87Combined sources10
Beta strandi97 – 101Combined sources5
Beta strandi127 – 131Combined sources5
Beta strandi136 – 143Combined sources8
Beta strandi148 – 153Combined sources6
Beta strandi156 – 158Combined sources3
Beta strandi160 – 166Combined sources7
Turni171 – 173Combined sources3
Beta strandi175 – 181Combined sources7
Helixi187 – 189Combined sources3
Helixi193 – 195Combined sources3
Helixi199 – 203Combined sources5
Beta strandi206 – 209Combined sources4
Helixi211 – 213Combined sources3
Turni216 – 218Combined sources3
Beta strandi219 – 223Combined sources5
Beta strandi226 – 228Combined sources3
Beta strandi231 – 233Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKKX-ray2.06B/D/F/H/J/L2-239[»]
1YKLX-ray2.25B/D/F/H/J/L2-239[»]
1YKMX-ray2.22B/D/F/H/J/L2-239[»]
1YKNX-ray2.06B/D/F/H/J/L2-239[»]
1YKOX-ray2.54B/D/F/H/J/L2-239[»]
1YKPX-ray2.41B/D/F/H/J/L2-239[»]
2PCDX-ray2.15M/N/O/P/Q/R2-239[»]
3LKTX-ray1.65M/N/O/P/Q/R2-239[»]
3LMXX-ray2.20M/N/O2-239[»]
3LXVX-ray1.90M/N/O2-239[»]
3MFLX-ray1.78M/N/O2-239[»]
3MI1X-ray1.74M/N/O2-239[»]
3MI5X-ray1.78M/N/O/P/Q/R2-239[»]
3MV4X-ray1.59M/N/O2-239[»]
3MV6X-ray1.86M/N/O2-239[»]
3PCAX-ray2.20M/N/O/P/Q/R2-239[»]
3PCBX-ray2.19M/N/O/P/Q/R2-239[»]
3PCCX-ray1.98M/N/O/P/Q/R2-239[»]
3PCDX-ray2.10M/N/O/P/Q/R2-239[»]
3PCEX-ray2.06M/N/O/P/Q/R2-239[»]
3PCFX-ray2.15M/N/O/P/Q/R2-239[»]
3PCGX-ray1.96M/N/O/P/Q/R2-239[»]
3PCHX-ray2.05M/N/O/P/Q/R2-239[»]
3PCIX-ray2.21M/N/O/P/Q/R2-239[»]
3PCJX-ray2.13M/N/O/P/Q/R2-239[»]
3PCKX-ray2.13M/N/O/P/Q/R2-239[»]
3PCLX-ray2.15M/N/O/P/Q/R2-239[»]
3PCMX-ray2.25M/N/O/P/Q/R2-239[»]
3PCNX-ray2.40M/N/O/P/Q/R2-239[»]
3T63X-ray1.54M/N/O2-239[»]
3T67X-ray1.67M/N/O2-239[»]
4WHOX-ray1.83B/D/F2-239[»]
4WHPX-ray1.54B/D/F2-239[»]
4WHQX-ray1.78B/D/F2-239[»]
4WHRX-ray1.58B/D/F2-239[»]
4WHSX-ray1.35B/D/F2-239[»]
ProteinModelPortaliP00437.
SMRiP00437.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00437.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EU7. Bacteria.
COG3485. LUCA.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAQDNSRFV IRDRNWHPKA LTPDYKTSIA RSPRQALVSI PQSISETTGP
60 70 80 90 100
NFSHLGFGAH DHDLLLNFNN GGLPIGERII VAGRVVDQYG KPVPNTLVEM
110 120 130 140 150
WQANAGGRYR HKNDRYLAPL DPNFGGVGRC LTDSDGYYSF RTIKPGPYPW
160 170 180 190 200
RNGPNDWRPA HIHFGISGPS IATKLITQLY FEGDPLIPMC PIVKSIANPE
210 220 230
AVQQLIAKLD MNNANPMDCL AYRFDIVLRG QRKTHFENC
Length:239
Mass (Da):26,793
Last modified:January 23, 2007 - v3
Checksum:i8F8CC293B6E434CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62H → D AA sequence (PubMed:115853).Curated1
Sequence conflicti70N → D AA sequence (PubMed:115853).Curated1
Sequence conflicti72Missing AA sequence (PubMed:115853).Curated1
Sequence conflicti218D → N AA sequence (PubMed:115853).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14836 Genomic DNA. Translation: AAB41024.1.
PIRiA36930. DAPSBA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14836 Genomic DNA. Translation: AAB41024.1.
PIRiA36930. DAPSBA.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKKX-ray2.06B/D/F/H/J/L2-239[»]
1YKLX-ray2.25B/D/F/H/J/L2-239[»]
1YKMX-ray2.22B/D/F/H/J/L2-239[»]
1YKNX-ray2.06B/D/F/H/J/L2-239[»]
1YKOX-ray2.54B/D/F/H/J/L2-239[»]
1YKPX-ray2.41B/D/F/H/J/L2-239[»]
2PCDX-ray2.15M/N/O/P/Q/R2-239[»]
3LKTX-ray1.65M/N/O/P/Q/R2-239[»]
3LMXX-ray2.20M/N/O2-239[»]
3LXVX-ray1.90M/N/O2-239[»]
3MFLX-ray1.78M/N/O2-239[»]
3MI1X-ray1.74M/N/O2-239[»]
3MI5X-ray1.78M/N/O/P/Q/R2-239[»]
3MV4X-ray1.59M/N/O2-239[»]
3MV6X-ray1.86M/N/O2-239[»]
3PCAX-ray2.20M/N/O/P/Q/R2-239[»]
3PCBX-ray2.19M/N/O/P/Q/R2-239[»]
3PCCX-ray1.98M/N/O/P/Q/R2-239[»]
3PCDX-ray2.10M/N/O/P/Q/R2-239[»]
3PCEX-ray2.06M/N/O/P/Q/R2-239[»]
3PCFX-ray2.15M/N/O/P/Q/R2-239[»]
3PCGX-ray1.96M/N/O/P/Q/R2-239[»]
3PCHX-ray2.05M/N/O/P/Q/R2-239[»]
3PCIX-ray2.21M/N/O/P/Q/R2-239[»]
3PCJX-ray2.13M/N/O/P/Q/R2-239[»]
3PCKX-ray2.13M/N/O/P/Q/R2-239[»]
3PCLX-ray2.15M/N/O/P/Q/R2-239[»]
3PCMX-ray2.25M/N/O/P/Q/R2-239[»]
3PCNX-ray2.40M/N/O/P/Q/R2-239[»]
3T63X-ray1.54M/N/O2-239[»]
3T67X-ray1.67M/N/O2-239[»]
4WHOX-ray1.83B/D/F2-239[»]
4WHPX-ray1.54B/D/F2-239[»]
4WHQX-ray1.78B/D/F2-239[»]
4WHRX-ray1.58B/D/F2-239[»]
4WHSX-ray1.35B/D/F2-239[»]
ProteinModelPortaliP00437.
SMRiP00437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00437. 1 interactor.
STRINGi160488.PP_4656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105EU7. Bacteria.
COG3485. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.
BioCyciMetaCyc:MONOMER-3185.

Miscellaneous databases

EvolutionaryTraceiP00437.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCXB_PSEPU
AccessioniPrimary (citable) accession number: P00437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain ATCC 23975 was originally classified as being from Pseudomonas aeruginosa.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.