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Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase beta chain (pcaH), Protocatechuate 3,4-dioxygenase alpha chain (pcaG)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134ProtocatechuateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3186.
UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaG
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000850952 – 201Protocatechuate 3,4-dioxygenase alpha chainAdd BLAST200

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Protein-protein interaction databases

IntActiP00436. 1 interactor.
STRINGi160488.PP_4655.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 22Combined sources5
Helixi24 – 27Combined sources4
Beta strandi45 – 47Combined sources3
Beta strandi49 – 57Combined sources9
Beta strandi68 – 72Combined sources5
Beta strandi88 – 90Combined sources3
Beta strandi92 – 97Combined sources6
Turni100 – 102Combined sources3
Beta strandi104 – 110Combined sources7
Beta strandi127 – 132Combined sources6
Beta strandi140 – 146Combined sources7
Helixi147 – 149Combined sources3
Helixi150 – 154Combined sources5
Helixi159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Helixi165 – 168Combined sources4
Helixi169 – 171Combined sources3
Beta strandi172 – 178Combined sources7
Beta strandi181 – 185Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKKX-ray2.06A/C/E/G/I/K2-201[»]
1YKLX-ray2.25A/C/E/G/I/K2-201[»]
1YKMX-ray2.22A/C/E/G/I/K2-201[»]
1YKNX-ray2.06A/C/E/G/I/K2-201[»]
1YKOX-ray2.54A/C/E/G/I/K2-201[»]
1YKPX-ray2.41A/C/E/G/I/K2-201[»]
2PCDX-ray2.15A/B/C/D/E/F2-201[»]
3LKTX-ray1.65A/B/C/D/E/F2-201[»]
3LMXX-ray2.20A/B/C2-201[»]
3LXVX-ray1.90A/B/C2-201[»]
3MFLX-ray1.78A/B/C2-201[»]
3MI1X-ray1.74A/B/C2-201[»]
3MI5X-ray1.78A/B/C/D/E/F2-201[»]
3MV4X-ray1.59A/B/C2-201[»]
3MV6X-ray1.86A/B/C2-201[»]
3PCAX-ray2.20A/B/C/D/E/F2-201[»]
3PCBX-ray2.19A/B/C/D/E/F2-201[»]
3PCCX-ray1.98A/B/C/D/E/F2-201[»]
3PCDX-ray2.10A/B/C/D/E/F2-201[»]
3PCEX-ray2.06A/B/C/D/E/F2-201[»]
3PCFX-ray2.15A/B/C/D/E/F2-201[»]
3PCGX-ray1.96A/B/C/D/E/F2-201[»]
3PCHX-ray2.05A/B/C/D/E/F2-201[»]
3PCIX-ray2.21A/B/C/D/E/F2-201[»]
3PCJX-ray2.13A/B/C/D/E/F2-201[»]
3PCKX-ray2.13A/B/C/D/E/F2-201[»]
3PCLX-ray2.15A/B/C/D/E/F2-201[»]
3PCMX-ray2.25A/B/C/D/E/F2-201[»]
3PCNX-ray2.40A/B/C/D/E/F2-201[»]
3T63X-ray1.54A/B/C2-201[»]
3T67X-ray1.67A/B/C2-201[»]
4WHOX-ray1.83A/C/E2-201[»]
4WHPX-ray1.54A/C/E2-201[»]
4WHQX-ray1.78A/C/E2-201[»]
4WHRX-ray1.58A/C/E2-201[»]
4WHSX-ray1.35A/C/E2-201[»]
ProteinModelPortaliP00436.
SMRiP00436.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00436.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105RM9. Bacteria.
COG3485. LUCA.

Family and domain databases

CDDicd03463. 3_4-PCD_alpha. 1 hit.
Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIELLPETP SQTAGPYVHI GLALEAAGNP TRDQEIWNRL AKPDAPGEHI
60 70 80 90 100
LLLGQVYDGN GHLVRDSFLE VWQADANGEY QDAYNLENAF NSFGRTATTF
110 120 130 140 150
DAGEWTLHTV KPGVVNNAAG VPMAPHINIS LFARGINIHL HTRLYFDDEA
160 170 180 190 200
QANAKCPVLN LIEQPQRRET LIAKRCEVDG KTAYRFDIRI QGEGETVFFD

F
Length:201
Mass (Da):22,387
Last modified:January 23, 2007 - v3
Checksum:iBF95DB892076FBAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60N → D AA sequence (PubMed:465136).Curated1
Sequence conflicti77N → D AA sequence (PubMed:465136).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14836 Genomic DNA. Translation: AAB41025.1.
PIRiB36930. DAPSAA.
RefSeqiWP_003251601.1. NZ_LWDW01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14836 Genomic DNA. Translation: AAB41025.1.
PIRiB36930. DAPSAA.
RefSeqiWP_003251601.1. NZ_LWDW01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKKX-ray2.06A/C/E/G/I/K2-201[»]
1YKLX-ray2.25A/C/E/G/I/K2-201[»]
1YKMX-ray2.22A/C/E/G/I/K2-201[»]
1YKNX-ray2.06A/C/E/G/I/K2-201[»]
1YKOX-ray2.54A/C/E/G/I/K2-201[»]
1YKPX-ray2.41A/C/E/G/I/K2-201[»]
2PCDX-ray2.15A/B/C/D/E/F2-201[»]
3LKTX-ray1.65A/B/C/D/E/F2-201[»]
3LMXX-ray2.20A/B/C2-201[»]
3LXVX-ray1.90A/B/C2-201[»]
3MFLX-ray1.78A/B/C2-201[»]
3MI1X-ray1.74A/B/C2-201[»]
3MI5X-ray1.78A/B/C/D/E/F2-201[»]
3MV4X-ray1.59A/B/C2-201[»]
3MV6X-ray1.86A/B/C2-201[»]
3PCAX-ray2.20A/B/C/D/E/F2-201[»]
3PCBX-ray2.19A/B/C/D/E/F2-201[»]
3PCCX-ray1.98A/B/C/D/E/F2-201[»]
3PCDX-ray2.10A/B/C/D/E/F2-201[»]
3PCEX-ray2.06A/B/C/D/E/F2-201[»]
3PCFX-ray2.15A/B/C/D/E/F2-201[»]
3PCGX-ray1.96A/B/C/D/E/F2-201[»]
3PCHX-ray2.05A/B/C/D/E/F2-201[»]
3PCIX-ray2.21A/B/C/D/E/F2-201[»]
3PCJX-ray2.13A/B/C/D/E/F2-201[»]
3PCKX-ray2.13A/B/C/D/E/F2-201[»]
3PCLX-ray2.15A/B/C/D/E/F2-201[»]
3PCMX-ray2.25A/B/C/D/E/F2-201[»]
3PCNX-ray2.40A/B/C/D/E/F2-201[»]
3T63X-ray1.54A/B/C2-201[»]
3T67X-ray1.67A/B/C2-201[»]
4WHOX-ray1.83A/C/E2-201[»]
4WHPX-ray1.54A/C/E2-201[»]
4WHQX-ray1.78A/C/E2-201[»]
4WHRX-ray1.58A/C/E2-201[»]
4WHSX-ray1.35A/C/E2-201[»]
ProteinModelPortaliP00436.
SMRiP00436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP00436. 1 interactor.
STRINGi160488.PP_4655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105RM9. Bacteria.
COG3485. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.
BioCyciMetaCyc:MONOMER-3186.

Miscellaneous databases

EvolutionaryTraceiP00436.

Family and domain databases

CDDicd03463. 3_4-PCD_alpha. 1 hit.
Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR012786. Protocat_dOase_a.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02423. protocat_alph. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCXA_PSEPU
AccessioniPrimary (citable) accession number: P00436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Strain ATCC 23975 was originally classified as being from Pseudomonas aeruginosa.Curated

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.