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Protein

Protocatechuate 3,4-dioxygenase alpha chain

Gene

pcaG

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Caution

Strain ATCC 23975 was originally classified as being from Pseudomonas aeruginosa.Curated

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase beta chain (pcaH), Protocatechuate 3,4-dioxygenase alpha chain (pcaG)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134ProtocatechuateSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processAromatic hydrocarbons catabolism
LigandIron

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-3186
UniPathwayiUPA00157; UER00264

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase alpha chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaG
OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
Taxonomic identifieri303 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000850952 – 201Protocatechuate 3,4-dioxygenase alpha chainAdd BLAST200

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Protein-protein interaction databases

IntActiP00436, 1 interactor

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 22Combined sources5
Helixi24 – 27Combined sources4
Beta strandi45 – 47Combined sources3
Beta strandi49 – 57Combined sources9
Beta strandi68 – 72Combined sources5
Beta strandi88 – 90Combined sources3
Beta strandi92 – 97Combined sources6
Turni100 – 102Combined sources3
Beta strandi104 – 110Combined sources7
Beta strandi127 – 132Combined sources6
Beta strandi140 – 146Combined sources7
Helixi147 – 149Combined sources3
Helixi150 – 154Combined sources5
Helixi159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Helixi165 – 168Combined sources4
Helixi169 – 171Combined sources3
Beta strandi172 – 178Combined sources7
Beta strandi181 – 185Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKKX-ray2.06A/C/E/G/I/K2-201[»]
1YKLX-ray2.25A/C/E/G/I/K2-201[»]
1YKMX-ray2.22A/C/E/G/I/K2-201[»]
1YKNX-ray2.06A/C/E/G/I/K2-201[»]
1YKOX-ray2.54A/C/E/G/I/K2-201[»]
1YKPX-ray2.41A/C/E/G/I/K2-201[»]
2PCDX-ray2.15A/B/C/D/E/F2-201[»]
3LKTX-ray1.65A/B/C/D/E/F2-201[»]
3LMXX-ray2.20A/B/C2-201[»]
3LXVX-ray1.90A/B/C2-201[»]
3MFLX-ray1.78A/B/C2-201[»]
3MI1X-ray1.74A/B/C2-201[»]
3MI5X-ray1.78A/B/C/D/E/F2-201[»]
3MV4X-ray1.59A/B/C2-201[»]
3MV6X-ray1.86A/B/C2-201[»]
3PCAX-ray2.20A/B/C/D/E/F2-201[»]
3PCBX-ray2.19A/B/C/D/E/F2-201[»]
3PCCX-ray1.98A/B/C/D/E/F2-201[»]
3PCDX-ray2.10A/B/C/D/E/F2-201[»]
3PCEX-ray2.06A/B/C/D/E/F2-201[»]
3PCFX-ray2.15A/B/C/D/E/F2-201[»]
3PCGX-ray1.96A/B/C/D/E/F2-201[»]
3PCHX-ray2.05A/B/C/D/E/F2-201[»]
3PCIX-ray2.21A/B/C/D/E/F2-201[»]
3PCJX-ray2.13A/B/C/D/E/F2-201[»]
3PCKX-ray2.13A/B/C/D/E/F2-201[»]
3PCLX-ray2.15A/B/C/D/E/F2-201[»]
3PCMX-ray2.25A/B/C/D/E/F2-201[»]
3PCNX-ray2.40A/B/C/D/E/F2-201[»]
3T63X-ray1.54A/B/C2-201[»]
3T67X-ray1.67A/B/C2-201[»]
4WHOX-ray1.83A/C/E2-201[»]
4WHPX-ray1.54A/C/E2-201[»]
4WHQX-ray1.78A/C/E2-201[»]
4WHRX-ray1.58A/C/E2-201[»]
4WHSX-ray1.35A/C/E2-201[»]
ProteinModelPortaliP00436
SMRiP00436
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00436

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105RM9 Bacteria
COG3485 LUCA

Family and domain databases

CDDicd03463 3_4-PCD_alpha, 1 hit
Gene3Di2.60.130.10, 1 hit
InterProiView protein in InterPro
IPR000627 Intradiol_dOase_C
IPR015889 Intradiol_dOase_core
IPR012786 Protocat_dOase_a
PfamiView protein in Pfam
PF00775 Dioxygenase_C, 1 hit
SUPFAMiSSF49482 SSF49482, 1 hit
TIGRFAMsiTIGR02423 protocat_alph, 1 hit
PROSITEiView protein in PROSITE
PS00083 INTRADIOL_DIOXYGENAS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIELLPETP SQTAGPYVHI GLALEAAGNP TRDQEIWNRL AKPDAPGEHI
60 70 80 90 100
LLLGQVYDGN GHLVRDSFLE VWQADANGEY QDAYNLENAF NSFGRTATTF
110 120 130 140 150
DAGEWTLHTV KPGVVNNAAG VPMAPHINIS LFARGINIHL HTRLYFDDEA
160 170 180 190 200
QANAKCPVLN LIEQPQRRET LIAKRCEVDG KTAYRFDIRI QGEGETVFFD

F
Length:201
Mass (Da):22,387
Last modified:January 23, 2007 - v3
Checksum:iBF95DB892076FBAF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60N → D AA sequence (PubMed:465136).Curated1
Sequence conflicti77N → D AA sequence (PubMed:465136).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14836 Genomic DNA Translation: AAB41025.1
PIRiB36930 DAPSAA
RefSeqiWP_003251601.1, NZ_PIJT01000012.1

Similar proteinsi

Entry informationi

Entry nameiPCXA_PSEPU
AccessioniPrimary (citable) accession number: P00436
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 117 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
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Main funding by: National Institutes of Health