P00425 (COX5B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase polypeptide 5B, mitochondrial
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide Vb

Gene names

Name:	COX5B
Ordered Locus Names:	YIL111W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytochrome c oxidase IV family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay PubMed 1331058. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial respiratory chain complex IV Inferred from direct assay Ref.5. Source: SGD
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 17	17	Mitochondrion Potential
	Chain	18 – 151	134	Cytochrome c oxidase polypeptide 5B, mitochondrial		PRO_0000006098

Sequences

	Sequence		Length	Mass (Da)
	P00425 [UniParc]. Last modified March 1, 1992. Version 3. Checksum: 566B4E69631CBDE0	FASTA	151	17,197
	10 20 30 40 50 60 MLRTSLTKGA RLTGTRFVQT KALSKATLTD LPERWENMPN LEQKEIADNL TERQKLPWKT 70 80 90 100 110 120 LNNEEIKAAW YISYGEWGPR RPVHGKGDVA FITKGVFLGL GISFGLFGLV RLLANPETPK 130 140 150 TMNREWQLKS DEYLKSKNAN PWGGYSQVQS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V." Cumsky M.G., Trueblood C.E., Ko C., Poyton R.O. Mol. Cell. Biol. 7:3511-3519(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Splicing of a yeast intron containing an unusual 5' junction sequence." Hodge M.R., Cumsky M.G. Mol. Cell. Biol. 9:2765-2770(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-79.
[5]	"Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase." Cumsky M.G., Ko C., Trueblood C.E., Poyton R.O. Proc. Natl. Acad. Sci. U.S.A. 82:2235-2239(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 18-85.
[6]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38125 Genomic DNA. Translation: CAA86269.1. M17799 Genomic DNA. Translation: AAA96310.1. M11140 Genomic DNA. Translation: AAA34521.1. BK006942 Genomic DNA. Translation: DAA08442.1.
PIR	OTBY5B. S05755.
RefSeq	NP_012155.1. NM_001179459.1.
3D structure databases
ProteinModelPortal	P00425.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4709N.
MINT	MINT-513424.
STRING	4932.YIL111W.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
Proteomic databases
PaxDb	P00425.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL111W; YIL111W; YIL111W.
GeneID	854695.
KEGG	sce:YIL111W.
Organism-specific databases
CYGD	YIL111w.
SGD	S000001373. COX5B.
Phylogenomic databases
eggNOG	NOG263759.
GeneTree	ENSGT00390000015571.
HOGENOM	HOG000175160.
KO	K02263.
OMA	QEQAELW.
OrthoDB	EOG4T1MWV.
Gene expression databases
Genevestigator	P00425.
GermOnline	YIL111W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.442.10. 1 hit.
InterPro	IPR004203. Cyt_c_oxidase_su4_fam. [Graphical view]
Pfam	PF02936. COX4. 1 hit. [Graphical view]
SUPFAM	SSF81406. COX4. 1 hit.
ProtoNet	Search...
Other
NextBio	977331.

Entry information

Entry name

COX5B_YEAST

Accession

Primary (citable) accession number: P00425
Secondary accession number(s): D6VVH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program