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P00424

- COX5A_YEAST

UniProt

P00424 - COX5A_YEAST

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Protein

Cytochrome c oxidase polypeptide 5A, mitochondrial

Gene

COX5A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. hydrogen ion transmembrane transport Source: GOC
  2. mitochondrial electron transport, cytochrome c to oxygen Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciMetaCyc:YNL052W-MONOMER.
YEAST:YNL052W-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase polypeptide 5A, mitochondrial (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide Va
Gene namesi
Name:COX5A
Ordered Locus Names:YNL052W
ORF Names:N2474, YNL2474W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL052w.
SGDiS000004997. COX5A.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Reactome
  2. mitochondrial intermembrane space Source: Reactome
  3. mitochondrial respiratory chain complex IV Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2020Mitochondrion2 PublicationsAdd
BLAST
Chaini21 – 153133Cytochrome c oxidase polypeptide 5A, mitochondrialPRO_0000006097Add
BLAST

Proteomic databases

MaxQBiP00424.
PaxDbiP00424.

Expressioni

Gene expression databases

GenevestigatoriP00424.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Protein-protein interaction databases

BioGridi35772. 112 interactions.
DIPiDIP-6820N.
IntActiP00424. 4 interactions.
MINTiMINT-672407.
STRINGi4932.YNL052W.

Structurei

3D structure databases

ProteinModelPortaliP00424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c oxidase IV family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiNOG263759.
GeneTreeiENSGT00390000015571.
HOGENOMiHOG000175160.
InParanoidiP00424.
KOiK02263.
OMAiCKENNAK.
OrthoDBiEOG7JHMJ1.

Family and domain databases

Gene3Di1.10.442.10. 1 hit.
InterProiIPR004203. Cyt_c_oxidase_su4_fam.
[Graphical view]
PfamiPF02936. COX4. 1 hit.
[Graphical view]
SUPFAMiSSF81406. SSF81406. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00424-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRNTFTRAG GLSRITSVRF AQTHALSNAA VMDLQSRWEN MPSTEQQDIV
60 70 80 90 100
SKLSERQKLP WAQLTEPEKQ AVWYISYGEW GPRRPVLNKG DSSFIAKGVA
110 120 130 140 150
AGLLFSVGLF AVVRMAGGQD AKTMNKEWQL KSDEYLKSKN ANPWGGYSQV

QSK
Length:153
Mass (Da):17,140
Last modified:August 13, 1987 - v1
Checksum:i1B1F8A3FE12CF783
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511Missing AA sequence (PubMed:6327686)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02561 Genomic DNA. Translation: CAA26403.1.
M11770 Genomic DNA. Translation: AAA34518.1.
M17800 Genomic DNA. Translation: AAA34520.1.
U12141 Genomic DNA. Translation: AAA99660.1.
Z71328 Genomic DNA. Translation: CAA95921.1.
AY558131 Genomic DNA. Translation: AAS56457.1.
M11141 Genomic DNA. Translation: AAA34519.1.
BK006947 Genomic DNA. Translation: DAA10493.1.
PIRiS05833. OTBY5A.
RefSeqiNP_014346.1. NM_001182891.1.

Genome annotation databases

EnsemblFungiiYNL052W; YNL052W; YNL052W.
GeneIDi855675.
KEGGisce:YNL052W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02561 Genomic DNA. Translation: CAA26403.1 .
M11770 Genomic DNA. Translation: AAA34518.1 .
M17800 Genomic DNA. Translation: AAA34520.1 .
U12141 Genomic DNA. Translation: AAA99660.1 .
Z71328 Genomic DNA. Translation: CAA95921.1 .
AY558131 Genomic DNA. Translation: AAS56457.1 .
M11141 Genomic DNA. Translation: AAA34519.1 .
BK006947 Genomic DNA. Translation: DAA10493.1 .
PIRi S05833. OTBY5A.
RefSeqi NP_014346.1. NM_001182891.1.

3D structure databases

ProteinModelPortali P00424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35772. 112 interactions.
DIPi DIP-6820N.
IntActi P00424. 4 interactions.
MINTi MINT-672407.
STRINGi 4932.YNL052W.

Protein family/group databases

TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBi P00424.
PaxDbi P00424.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL052W ; YNL052W ; YNL052W .
GeneIDi 855675.
KEGGi sce:YNL052W.

Organism-specific databases

CYGDi YNL052w.
SGDi S000004997. COX5A.

Phylogenomic databases

eggNOGi NOG263759.
GeneTreei ENSGT00390000015571.
HOGENOMi HOG000175160.
InParanoidi P00424.
KOi K02263.
OMAi CKENNAK.
OrthoDBi EOG7JHMJ1.

Enzyme and pathway databases

BioCyci MetaCyc:YNL052W-MONOMER.
YEAST:YNL052W-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 979963.

Gene expression databases

Genevestigatori P00424.

Family and domain databases

Gene3Di 1.10.442.10. 1 hit.
InterProi IPR004203. Cyt_c_oxidase_su4_fam.
[Graphical view ]
Pfami PF02936. COX4. 1 hit.
[Graphical view ]
SUPFAMi SSF81406. SSF81406. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of a gene for subunit V of the cytochrome c oxidase from Saccharomyces cerevisiae."
    Seraphin B., Simon M., Faye G.
    Curr. Genet. 9:435-439(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and characterization of the yeast nuclear gene for subunit 5 of cytochrome oxidase."
    Koerner T.J., Hill J., Tzagoloff A.
    J. Biol. Chem. 260:9513-9515(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V."
    Cumsky M.G., Trueblood C.E., Ko C., Poyton R.O.
    Mol. Cell. Biol. 7:3511-3519(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  8. "Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase."
    Cumsky M.G., Ko C., Trueblood C.E., Poyton R.O.
    Proc. Natl. Acad. Sci. U.S.A. 82:2235-2239(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-98.
  9. "The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases."
    Power S.D., Lochrie M.A., Poyton R.O.
    J. Biol. Chem. 259:6575-6578(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-69.
  10. "Import of proteins into mitochondria. Isolated yeast mitochondria and a solubilized matrix protease correctly process cytochrome c oxidase subunit V precursor at the NH2 terminus."
    Cerletti N., Bohni P.C., Suda K.
    J. Biol. Chem. 258:4944-4949(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-35.
  11. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCOX5A_YEAST
AccessioniPrimary (citable) accession number: P00424
Secondary accession number(s): D6W1C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: October 29, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3