P00424 (COX5A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase polypeptide 5A, mitochondrial
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide Va

Gene names

Name:	COX5A
Ordered Locus Names:	YNL052W
ORF Names:	N2474, YNL2474W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	Present with 3670 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the cytochrome c oxidase IV family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay PubMed 1331058. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial intermembrane space Traceable author statement. Source: Reactome mitochondrial respiratory chain complex IV Inferred from physical interaction PubMed 1331058. Source: SGD
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PHO88	P38264	1	EBI-5345,EBI-13350

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 20	20	Mitochondrion Ref.10 Ref.11
	Chain	21 – 153	133	Cytochrome c oxidase polypeptide 5A, mitochondrial		PRO_0000006097
Experimental info
	Sequence conflict	51	1	Missing AA sequence Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P00424 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 1B1F8A3FE12CF783	FASTA	153	17,140
	10 20 30 40 50 60 MLRNTFTRAG GLSRITSVRF AQTHALSNAA VMDLQSRWEN MPSTEQQDIV SKLSERQKLP 70 80 90 100 110 120 WAQLTEPEKQ AVWYISYGEW GPRRPVLNKG DSSFIAKGVA AGLLFSVGLF AVVRMAGGQD 130 140 150 AKTMNKEWQL KSDEYLKSKN ANPWGGYSQV QSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of a gene for subunit V of the cytochrome c oxidase from Saccharomyces cerevisiae." Seraphin B., Simon M., Faye G. Curr. Genet. 9:435-439(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and characterization of the yeast nuclear gene for subunit 5 of cytochrome oxidase." Koerner T.J., Hill J., Tzagoloff A. J. Biol. Chem. 260:9513-9515(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V." Cumsky M.G., Trueblood C.E., Ko C., Poyton R.O. Mol. Cell. Biol. 7:3511-3519(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae." Bergez P., Doignon F., Crouzet M. Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[5]	Erratum Bergez P., Doignon F., Crouzet M. Yeast 12:297-297(1996) [PubMed] [Europe PMC] [Abstract]
[6]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S288c / FY1676.
[7]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[8]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[9]	"Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase." Cumsky M.G., Ko C., Trueblood C.E., Poyton R.O. Proc. Natl. Acad. Sci. U.S.A. 82:2235-2239(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-98.
[10]	"The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases." Power S.D., Lochrie M.A., Poyton R.O. J. Biol. Chem. 259:6575-6578(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-69.
[11]	"Import of proteins into mitochondria. Isolated yeast mitochondria and a solubilized matrix protease correctly process cytochrome c oxidase subunit V precursor at the NH2 terminus." Cerletti N., Bohni P.C., Suda K. J. Biol. Chem. 258:4944-4949(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-35.
[12]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02561 Genomic DNA. Translation: CAA26403.1. M11770 Genomic DNA. Translation: AAA34518.1. M17800 Genomic DNA. Translation: AAA34520.1. U12141 Genomic DNA. Translation: AAA99660.1. Z71328 Genomic DNA. Translation: CAA95921.1. AY558131 Genomic DNA. Translation: AAS56457.1. M11141 Genomic DNA. Translation: AAA34519.1. BK006947 Genomic DNA. Translation: DAA10493.1.
PIR	OTBY5A. S05833.
RefSeq	NP_014346.1. NM_001182891.1.
3D structure databases
ProteinModelPortal	P00424.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6820N.
IntAct	P00424. 3 interactions.
MINT	MINT-672407.
STRING	4932.YNL052W.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
Proteomic databases
PaxDb	P00424.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL052W; YNL052W; YNL052W.
GeneID	855675.
KEGG	sce:YNL052W.
Organism-specific databases
CYGD	YNL052w.
SGD	S000004997. COX5A.
Phylogenomic databases
eggNOG	NOG263759.
GeneTree	ENSGT00390000015571.
HOGENOM	HOG000175160.
KO	K02263.
OMA	QSSHAIS.
OrthoDB	EOG4T1MWV.
Enzyme and pathway databases
Reactome	REACT_118590. Mitochondrial Protein Import (yeast). REACT_85873. Metabolism of proteins.
Gene expression databases
Genevestigator	P00424.
GermOnline	YNL052W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.442.10. 1 hit.
InterPro	IPR004203. Cyt_c_oxidase_su4_fam. [Graphical view]
Pfam	PF02936. COX4. 1 hit. [Graphical view]
SUPFAM	SSF81406. COX4. 1 hit.
ProtoNet	Search...
Other
NextBio	979963.

Entry information

Entry name

COX5A_YEAST

Accession

Primary (citable) accession number: P00424
Secondary accession number(s): D6W1C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 126 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program