Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00424

- COX5A_YEAST

UniProt

P00424 - COX5A_YEAST

Protein

Cytochrome c oxidase polypeptide 5A, mitochondrial

Gene

COX5A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. hydrogen ion transmembrane transport Source: GOC
    2. mitochondrial electron transport, cytochrome c to oxygen Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Enzyme and pathway databases

    BioCyciMetaCyc:YNL052W-MONOMER.
    YEAST:YNL052W-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase polypeptide 5A, mitochondrial (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide Va
    Gene namesi
    Name:COX5A
    Ordered Locus Names:YNL052W
    ORF Names:N2474, YNL2474W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL052w.
    SGDiS000004997. COX5A.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrial intermembrane space Source: Reactome
    3. mitochondrial respiratory chain complex IV Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2020Mitochondrion2 PublicationsAdd
    BLAST
    Chaini21 – 153133Cytochrome c oxidase polypeptide 5A, mitochondrialPRO_0000006097Add
    BLAST

    Proteomic databases

    MaxQBiP00424.
    PaxDbiP00424.

    Expressioni

    Gene expression databases

    GenevestigatoriP00424.

    Interactioni

    Subunit structurei

    Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

    Protein-protein interaction databases

    BioGridi35772. 111 interactions.
    DIPiDIP-6820N.
    IntActiP00424. 4 interactions.
    MINTiMINT-672407.
    STRINGi4932.YNL052W.

    Structurei

    3D structure databases

    ProteinModelPortaliP00424.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome c oxidase IV family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiNOG263759.
    GeneTreeiENSGT00390000015571.
    HOGENOMiHOG000175160.
    KOiK02263.
    OMAiCKENNAK.
    OrthoDBiEOG7JHMJ1.

    Family and domain databases

    Gene3Di1.10.442.10. 1 hit.
    InterProiIPR004203. Cyt_c_oxidase_su4_fam.
    [Graphical view]
    PfamiPF02936. COX4. 1 hit.
    [Graphical view]
    SUPFAMiSSF81406. SSF81406. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00424-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRNTFTRAG GLSRITSVRF AQTHALSNAA VMDLQSRWEN MPSTEQQDIV    50
    SKLSERQKLP WAQLTEPEKQ AVWYISYGEW GPRRPVLNKG DSSFIAKGVA 100
    AGLLFSVGLF AVVRMAGGQD AKTMNKEWQL KSDEYLKSKN ANPWGGYSQV 150
    QSK 153
    Length:153
    Mass (Da):17,140
    Last modified:August 13, 1987 - v1
    Checksum:i1B1F8A3FE12CF783
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511Missing AA sequence (PubMed:6327686)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02561 Genomic DNA. Translation: CAA26403.1.
    M11770 Genomic DNA. Translation: AAA34518.1.
    M17800 Genomic DNA. Translation: AAA34520.1.
    U12141 Genomic DNA. Translation: AAA99660.1.
    Z71328 Genomic DNA. Translation: CAA95921.1.
    AY558131 Genomic DNA. Translation: AAS56457.1.
    M11141 Genomic DNA. Translation: AAA34519.1.
    BK006947 Genomic DNA. Translation: DAA10493.1.
    PIRiS05833. OTBY5A.
    RefSeqiNP_014346.1. NM_001182891.1.

    Genome annotation databases

    EnsemblFungiiYNL052W; YNL052W; YNL052W.
    GeneIDi855675.
    KEGGisce:YNL052W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02561 Genomic DNA. Translation: CAA26403.1 .
    M11770 Genomic DNA. Translation: AAA34518.1 .
    M17800 Genomic DNA. Translation: AAA34520.1 .
    U12141 Genomic DNA. Translation: AAA99660.1 .
    Z71328 Genomic DNA. Translation: CAA95921.1 .
    AY558131 Genomic DNA. Translation: AAS56457.1 .
    M11141 Genomic DNA. Translation: AAA34519.1 .
    BK006947 Genomic DNA. Translation: DAA10493.1 .
    PIRi S05833. OTBY5A.
    RefSeqi NP_014346.1. NM_001182891.1.

    3D structure databases

    ProteinModelPortali P00424.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35772. 111 interactions.
    DIPi DIP-6820N.
    IntActi P00424. 4 interactions.
    MINTi MINT-672407.
    STRINGi 4932.YNL052W.

    Protein family/group databases

    TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Proteomic databases

    MaxQBi P00424.
    PaxDbi P00424.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL052W ; YNL052W ; YNL052W .
    GeneIDi 855675.
    KEGGi sce:YNL052W.

    Organism-specific databases

    CYGDi YNL052w.
    SGDi S000004997. COX5A.

    Phylogenomic databases

    eggNOGi NOG263759.
    GeneTreei ENSGT00390000015571.
    HOGENOMi HOG000175160.
    KOi K02263.
    OMAi CKENNAK.
    OrthoDBi EOG7JHMJ1.

    Enzyme and pathway databases

    BioCyci MetaCyc:YNL052W-MONOMER.
    YEAST:YNL052W-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 979963.

    Gene expression databases

    Genevestigatori P00424.

    Family and domain databases

    Gene3Di 1.10.442.10. 1 hit.
    InterProi IPR004203. Cyt_c_oxidase_su4_fam.
    [Graphical view ]
    Pfami PF02936. COX4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81406. SSF81406. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a gene for subunit V of the cytochrome c oxidase from Saccharomyces cerevisiae."
      Seraphin B., Simon M., Faye G.
      Curr. Genet. 9:435-439(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and characterization of the yeast nuclear gene for subunit 5 of cytochrome oxidase."
      Koerner T.J., Hill J., Tzagoloff A.
      J. Biol. Chem. 260:9513-9515(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Structural analysis of two genes encoding divergent forms of yeast cytochrome c oxidase subunit V."
      Cumsky M.G., Trueblood C.E., Ko C., Poyton R.O.
      Mol. Cell. Biol. 7:3511-3519(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    8. "Two nonidentical forms of subunit V are functional in yeast cytochrome c oxidase."
      Cumsky M.G., Ko C., Trueblood C.E., Poyton R.O.
      Proc. Natl. Acad. Sci. U.S.A. 82:2235-2239(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-98.
    9. "The nuclear-coded subunits of yeast cytochrome c oxidase. III. Identification of homologous subunits in yeast, bovine heart, and Neurospora crassa cytochrome c oxidases."
      Power S.D., Lochrie M.A., Poyton R.O.
      J. Biol. Chem. 259:6575-6578(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-69.
    10. "Import of proteins into mitochondria. Isolated yeast mitochondria and a solubilized matrix protease correctly process cytochrome c oxidase subunit V precursor at the NH2 terminus."
      Cerletti N., Bohni P.C., Suda K.
      J. Biol. Chem. 258:4944-4949(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-35.
    11. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
      Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
      Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCOX5A_YEAST
    AccessioniPrimary (citable) accession number: P00424
    Secondary accession number(s): D6W1C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3670 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3