P00422 (COX3_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 3
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide III

Gene names

Name:	cox-3
Synonyms:	cox3, oxi2
ORF Names:	NCM012, NCU16003

Encoded on

Mitochondrion

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunits I, II and III form the functional core of the enzyme complex.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 3 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Cytochrome c oxidase subunit 3		PRO_0000183813
Regions
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	138 – 160	23	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P00422 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: DADCF118933781BC	FASTA	269	30,089
	10 20 30 40 50 60 MTNLIRSNFQ DHPFHLVSPS PWPLNTSVCL LNLTTTGALS MHNFNNIHYL YYIALIGLVS 70 80 90 100 110 120 AMFLWFRDII SEGTFLGDHT LAVQRGLNLG IILFIVSEAL FFLAIFWAFF HSALTPTVEL 130 140 150 160 170 180 GAQWPPIGIE PVNPFELPLL NTVILLSSGA TITYAHHALI KGEREGALYG SIATILLAII 190 200 210 220 230 240 FTGFQGVEYS VSSFTISDGA FGTCFFFSTG FHGIHVIIGT IFLAVALWRI FAYHLTDNHH 250 260 VGFEGGILYW HFVDVVWLFL YISVYYWGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytochrome oxidase subunit III gene in Neurospora crassa mitochondria. Location and sequence." Browning K.S., RajBhandary U.L. J. Biol. Chem. 257:5253-5256(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"Mitochondrial genetics of Neurospora." Kennell J.C., Collins R.A., Griffiths A.J.F., Nargang F.E. (In) Kueck U. (eds.); The Mycota II, Genetics and Biotechnology (2nd edition), pp.95-112, Springer-Verlag, Berlin-Heidelberg (2004) Cited for: GENOME REANNOTATION. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]	"Products of mitochondrial protein synthesis in Neurospora crassa. Determination of equimolar amounts of three products in cytochrome oxidase on the basis of amino-acid analysis." Sebald W., Machleidt W., Otto J. Eur. J. Biochem. 38:311-324(1973) [PubMed] [Europe PMC] [Abstract] Cited for: AMINO-ACID COMPOSITION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00668 Genomic DNA. Translation: CAA24041.1.
PIR	OTNC3. A00489.
3D structure databases
ProteinModelPortal	P00422.
SMR	P00422. Positions 10-269.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
OrthoDB	EOG4578GM.
Family and domain databases
Gene3D	1.20.120.80. 1 hit.
InterPro	IPR024791. Cyt_c/ubiquinol_Oxase_su3. IPR000298. Cyt_c_oxidase_su3. IPR013833. Cyt_c_oxidase_su3_a-hlx. [Graphical view]
PANTHER	PTHR11403. PTHR11403. 1 hit.
Pfam	PF00510. COX3. 1 hit. [Graphical view]
SUPFAM	SSF81452. CytC_oxdse_III. 1 hit.
PROSITE	PS50253. COX3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX3_NEUCR

Accession

Primary (citable) accession number: P00422

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program