P00420 (COX3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 3
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide III

Gene names

Name:	COX3
Synonyms:	OXI2
Ordered Locus Names:	Q0275

Encoded on

Mitochondrion

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Subunits I, II and III form the functional core of the enzyme complex.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 3 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype. Source: SGD mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex IV Inferred from physical interaction. Source: SGD
Molecular function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Cytochrome c oxidase subunit 3		PRO_0000183874
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	45 – 65	21	Helical; Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Transmembrane	204 – 224	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
Experimental info
	Sequence conflict	263	1	V → T in AAA32153. Ref.1
	Sequence conflict	263	1	V → T in AAA67530. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00420 [UniParc]. Last modified April 23, 2003. Version 3. Checksum: 81B24A54F63BD8B1	FASTA	269	30,360
	10 20 30 40 50 60 MTHLERSRHQ QHPFHMVMPS PWPIVVSFAL LSLALSTALT MHGYIGNMNM VYLALFVLLT 70 80 90 100 110 120 SSILWFRDIV AEATYLGDHT MAVRKGINLG FLMFVLSEVL IFAGLFWAYF HSAMSPDVTL 130 140 150 160 170 180 GACWPPVGIE AVQPTELPLL NTIILLSSGA TVTYSHHALI AGNRNKALSG LLITFWLIVI 190 200 210 220 230 240 FVTCQYIEYT NAAFTISDGV YGSVFYAGTG LHFLHMVMLA AMLGVNYWRM RNYHLTAGHH 250 260 VGYETTIIYT HVLDVIWLFL YVVFYWWGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assembly of the mitochondrial membrane system. Sequence of the oxi 2 gene of yeast mitochondrial DNA." Thalenfeld B.E., Tzagoloff A. J. Biol. Chem. 255:6173-6180(1980) [PubMed: 6248512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D273-10B/A48.
[2]	"The primary structure of the mitochondrial genome of Saccharomyces cerevisiae -- a review." de Zamaroczy M., Bernardi G. Gene 47:155-177(1986) [PubMed: 3549452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae." Foury F., Roganti T., Lecrenier N., Purnelle B. FEBS Lett. 440:325-331(1998) [PubMed: 9872396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed: 7851399] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01478 Genomic DNA. Translation: AAA32153.2. L36890 Genomic DNA. Translation: AAA67530.1. AJ011856 Genomic DNA. Translation: CAA09846.1.
PIR	OTBY3. A00487. S78686.
RefSeq	NP_009328.1. NM_001184375.1.
3D structure databases
ProteinModelPortal	P00420.
SMR	P00420. Positions 8-268.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8133N.
IntAct	P00420. 1 interaction.
STRING	P00420.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	Q0275; Q0275; Q0275.
GeneID	854627.
KEGG	sce:Q0275.
NMPDR	fig|4932.3.peg.1880.
Organism-specific databases
CYGD	Q0275.
SGD	S000007283. COX3.
Phylogenomic databases
eggNOG	fuNOG07984.
HOGENOM	HBG565518.
OrthoDB	EOG4578GM.
Gene expression databases
ArrayExpress	P00420.
Genevestigator	P00420.
GermOnline	Q0275. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024791. Cyt_c/ubiquinol_Oxase_su3. IPR000298. Cyt_c_oxidase_su3. IPR013833. Cyt_c_oxidase_su3_a-hlx. [Graphical view]
Gene3D	G3DSA:1.20.120.80. CytC_oxdse_III_a-bundle. 1 hit.
KO	K02262.
PANTHER	PTHR11403. CytC_oxdse_III. 1 hit.
Pfam	PF00510. COX3. 1 hit. [Graphical view]
SUPFAM	SSF81452. CytC_oxdse_III. 1 hit.
PROSITE	PS50253. COX3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977142.

Entry information

Entry name

COX3_YEAST

Accession

Primary (citable) accession number: P00420
Secondary accession number(s): Q9ZZV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	April 23, 2003
Last modified:	January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program