P00413 (COX2_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 2
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide II

Gene names

Name:	COX2
Synonyms:	COII, COXII

Encoded on

Mitochondrion

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Copper A.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.
RNA editing	Edited at positions 26, 56, 57, 87, 129, 130, 188, 196, 207, 213, 228 and 235. Ref.2 Ref.3

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Cytochrome c oxidase subunit 2		PRO_0000183714
Regions
	Topological domain	1 – 43	43	Mitochondrial intermembrane Potential
	Transmembrane	44 – 64	21	Helical; Potential
	Topological domain	65 – 84	20	Mitochondrial matrix Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Topological domain	106 – 260	155	Mitochondrial intermembrane Potential
Sites
	Metal binding	189	1	Copper A Probable
	Metal binding	224	1	Copper A Probable
	Metal binding	228	1	Copper A Probable
	Metal binding	232	1	Copper A Probable

Sequences

	Sequence		Length	Mass (Da)
	P00413 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: 272F3C4573DA066D	FASTA	260	29,515
	10 20 30 40 50 60 MILRSLSCRF LTIALCDAAE PWQLGFQDAA TPMMQGIIDL HHDIFFFLIL ILVFVLWMLV 70 80 90 100 110 120 RALWHFNEQT NPIPQRIVHG TTIEIIWTIF PSVILLFIAI PSFALLYSMD GVLVDPAITI 130 140 150 160 170 180 KAIGHQWYWT YEYSDYNSSD EQSLTFDSYT IPEDDPELGQ SRLLEVDNRV VVPAKTHLRM 190 200 210 220 230 240 IVTPADVLHS WAVPSLGVKC DAVPGRLNLT SILVQREGVY YGQCSEICGT NHAFMPIVVE 250 260 AVTLKDYADW VSNQLILQTN

References

[1]	"The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize." Bonen L., Boer P.H., Gray M.W. EMBO J. 3:2531-2536(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"RNA editing in wheat mitochondria results in the conservation of protein sequences." Gualberto J.M., Lamattina L., Bonnard G., Weil J.-H., Grienenberger J.-M. Nature 341:660-662(1989) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.
[3]	"RNA editing in plant mitochondria." Covello P.S., Gray M.W. Nature 341:662-666(1989) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01108 Genomic DNA. Translation: CAA25581.1. Sequence problems.
PIR	OBWT2. A00481.
RefSeq	YP_398417.1. NC_007579.1.
3D structure databases
ProteinModelPortal	P00413.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3800085.
Organism-specific databases
Gramene	P00413.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR014222. Cyt_c_oxidase_su2. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Pfam	PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
TIGRFAMs	TIGR02866. CoxB. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX2_WHEAT

Accession

Primary (citable) accession number: P00413

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program