Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P00410

- COX2_YEAST

UniProt

P00410 - COX2_YEAST

Protein

Cytochrome c oxidase subunit 2

Gene

COX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Cofactori

    Copper A.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei15 – 162Cleavage; by IMP1
    Metal bindingi186 – 1861Copper ACurated
    Metal bindingi221 – 2211Copper ACurated
    Metal bindingi225 – 2251Copper ACurated
    Metal bindingi229 – 2291Copper ACurated

    GO - Molecular functioni

    1. copper ion binding Source: InterPro
    2. cytochrome-c oxidase activity Source: UniProtKB-EC
    3. protein binding Source: IntAct

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. hydrogen ion transmembrane transport Source: GOC
    3. mitochondrial electron transport, cytochrome c to oxygen Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:Q0250-MONOMER.
    YEAST:Q0250-MONOMER.

    Protein family/group databases

    TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide II
    Gene namesi
    Name:COX2
    Synonyms:OXI1
    Ordered Locus Names:Q0250
    Encoded oniMitochondrion
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Mitochondrion

    Organism-specific databases

    CYGDiQ0250.
    SGDiS000007281. COX2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial respiratory chain complex IV Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1515Removed in mature formPRO_0000419180Add
    BLAST
    Chaini16 – 251236Cytochrome c oxidase subunit 2PRO_0000006048Add
    BLAST

    Post-translational modificationi

    The N-terminal sequence of COX2 is processed by IMP1.

    Proteomic databases

    MaxQBiP00410.
    PaxDbiP00410.
    PeptideAtlasiP00410.

    Expressioni

    Gene expression databases

    GenevestigatoriP00410.

    Interactioni

    Subunit structurei

    Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    COX20Q049353EBI-5024,EBI-36910

    Protein-protein interaction databases

    BioGridi34812. 35 interactions.
    DIPiDIP-7592N.
    IntActiP00410. 9 interactions.
    MINTiMINT-434441.

    Structurei

    3D structure databases

    ProteinModelPortaliP00410.
    SMRiP00410. Positions 20-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini16 – 4227Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini65 – 8218Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini108 – 251144Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei43 – 6422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10725HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1622.
    GeneTreeiENSGT00390000017410.
    HOGENOMiHOG000264988.
    KOiK02261.
    OMAiNHEMISS.
    OrthoDBiEOG7WDNDC.

    Family and domain databases

    Gene3Di1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProiIPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view]
    PfamiPF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view]
    SUPFAMiSSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsiTIGR02866. CoxB. 1 hit.
    PROSITEiPS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00410-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI    50
    LGLVSWMLYT IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS 100
    FILLYLCDEV ISPAMTIKAI GYQWYWKYEY SDFINDSGET VEFESYVIPD 150
    ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT AADVIHDFAI PSLGIKVDAT 200
    PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS LPKFLEWLNE 250
    Q 251
    Length:251
    Mass (Da):28,567
    Last modified:July 21, 1986 - v1
    Checksum:iBA5717C37330DF41
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti59 – 591Y → N in AAA32155. (PubMed:2547760)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02200 Genomic DNA. Translation: AAA32158.2.
    V00706 Genomic DNA. Translation: CAA24078.1.
    V00685 Genomic DNA. Translation: CAA24056.1.
    AJ011856 Genomic DNA. Translation: CAA09845.1.
    J05007 Genomic DNA. Translation: AAA32155.2.
    L36888 Genomic DNA. Translation: AAA67528.1.
    D55725 Genomic DNA. Translation: BAA09539.1.
    PIRiA00478. OBBY2.
    RefSeqiNP_009326.1. NM_001184373.1.

    Genome annotation databases

    EnsemblFungiiQ0250; Q0250; Q0250.
    GeneIDi854622.
    KEGGisce:Q0250.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02200 Genomic DNA. Translation: AAA32158.2 .
    V00706 Genomic DNA. Translation: CAA24078.1 .
    V00685 Genomic DNA. Translation: CAA24056.1 .
    AJ011856 Genomic DNA. Translation: CAA09845.1 .
    J05007 Genomic DNA. Translation: AAA32155.2 .
    L36888 Genomic DNA. Translation: AAA67528.1 .
    D55725 Genomic DNA. Translation: BAA09539.1 .
    PIRi A00478. OBBY2.
    RefSeqi NP_009326.1. NM_001184373.1.

    3D structure databases

    ProteinModelPortali P00410.
    SMRi P00410. Positions 20-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34812. 35 interactions.
    DIPi DIP-7592N.
    IntActi P00410. 9 interactions.
    MINTi MINT-434441.

    Protein family/group databases

    TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Proteomic databases

    MaxQBi P00410.
    PaxDbi P00410.
    PeptideAtlasi P00410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii Q0250 ; Q0250 ; Q0250 .
    GeneIDi 854622.
    KEGGi sce:Q0250.

    Organism-specific databases

    CYGDi Q0250.
    SGDi S000007281. COX2.

    Phylogenomic databases

    eggNOGi COG1622.
    GeneTreei ENSGT00390000017410.
    HOGENOMi HOG000264988.
    KOi K02261.
    OMAi NHEMISS.
    OrthoDBi EOG7WDNDC.

    Enzyme and pathway databases

    BioCyci MetaCyc:Q0250-MONOMER.
    YEAST:Q0250-MONOMER.

    Miscellaneous databases

    NextBioi 977130.
    PROi P00410.

    Gene expression databases

    Genevestigatori P00410.

    Family and domain databases

    Gene3Di 1.10.287.90. 1 hit.
    2.60.40.420. 1 hit.
    InterProi IPR001505. Copper_CuA.
    IPR008972. Cupredoxin.
    IPR014222. Cyt_c_oxidase_su2.
    IPR002429. Cyt_c_oxidase_su2_C.
    IPR011759. Cyt_c_oxidase_su2_TM_dom.
    [Graphical view ]
    Pfami PF00116. COX2. 1 hit.
    PF02790. COX2_TM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49503. SSF49503. 1 hit.
    SSF81464. SSF81464. 1 hit.
    TIGRFAMsi TIGR02866. CoxB. 1 hit.
    PROSITEi PS00078. COX2. 1 hit.
    PS50857. COX2_CUA. 1 hit.
    PS50999. COX2_TM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase."
      Coruzzi G., Tzagoloff A.
      J. Biol. Chem. 254:9324-9330(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DS200/A1.
    2. "Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II."
      Fox T.D.
      Proc. Natl. Acad. Sci. U.S.A. 76:6534-6538(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B.
    3. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
      Foury F., Roganti T., Lecrenier N., Purnelle B.
      FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2."
      Cameron V.L., Fox T.D., Poyton R.O.
      J. Biol. Chem. 264:13391-13394(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
    5. "Use of the UGA terminator as a tryptophan codon in yeast mitochondria."
      Macino G., Coruzzi G., Nobrega F.G., Li M., Tzagoloff A.
      Proc. Natl. Acad. Sci. U.S.A. 76:3784-3785(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 121-132.
    6. Nakagawa Y.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-200.
      Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
    7. "A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae."
      Pratje E., Mannhaupt G., Michaelis G., Beyreuther K.
      EMBO J. 2:1049-1054(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSTRANSLATIONAL CLEAVAGE.
    8. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
      Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
      Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
    9. "SOM1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae."
      Esser K., Pratje E., Michaelis G.
      Mol. Gen. Genet. 252:437-445(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSTRANSLATIONAL CLEAVAGE BY IMP1.

    Entry informationi

    Entry nameiCOX2_YEAST
    AccessioniPrimary (citable) accession number: P00410
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3