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P00410 (COX2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase subunit 2

EC=1.9.3.1
Alternative name(s):
Cytochrome c oxidase polypeptide II
Gene names
Name:COX2
Synonyms:OXI1
Ordered Locus Names:Q0250
Encoded onMitochondrion
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactor

Copper A.

Subunit structure

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Post-translational modification

The N-terminal sequence of COX2 is processed by IMP1.

Sequence similarities

Belongs to the cytochrome c oxidase subunit 2 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

COX20Q049353EBI-5024,EBI-36910

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1515Removed in mature form
PRO_0000419180
Chain16 – 251236Cytochrome c oxidase subunit 2
PRO_0000006048

Regions

Topological domain16 – 4227Mitochondrial intermembrane Potential
Transmembrane43 – 6422Helical; Potential
Topological domain65 – 8218Mitochondrial matrix Potential
Transmembrane83 – 10725Helical; Potential
Topological domain108 – 251144Mitochondrial intermembrane Potential

Sites

Metal binding1861Copper A Probable
Metal binding2211Copper A Probable
Metal binding2251Copper A Probable
Metal binding2291Copper A Probable
Site15 – 162Cleavage; by IMP1

Experimental info

Sequence conflict591Y → N in AAA32155. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P00410 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: BA5717C37330DF41

FASTA25128,567
        10         20         30         40         50         60 
MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI LGLVSWMLYT 

        70         80         90        100        110        120 
IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS FILLYLCDEV ISPAMTIKAI 

       130        140        150        160        170        180 
GYQWYWKYEY SDFINDSGET VEFESYVIPD ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT 

       190        200        210        220        230        240 
AADVIHDFAI PSLGIKVDAT PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS 

       250 
LPKFLEWLNE Q 

« Hide

References

« Hide 'large scale' references
[1]"Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase."
Coruzzi G., Tzagoloff A.
J. Biol. Chem. 254:9324-9330(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DS200/A1.
[2]"Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II."
Fox T.D.
Proc. Natl. Acad. Sci. U.S.A. 76:6534-6538(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24657 / D273-10B.
[3]"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
Foury F., Roganti T., Lecrenier N., Purnelle B.
FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2."
Cameron V.L., Fox T.D., Poyton R.O.
J. Biol. Chem. 264:13391-13394(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
[5]"Use of the UGA terminator as a tryptophan codon in yeast mitochondria."
Macino G., Coruzzi G., Nobrega F.G., Li M., Tzagoloff A.
Proc. Natl. Acad. Sci. U.S.A. 76:3784-3785(1979) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 121-132.
[6]Nakagawa Y.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-200.
Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
[7]"A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae."
Pratje E., Mannhaupt G., Michaelis G., Beyreuther K.
EMBO J. 2:1049-1054(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: POSTRANSLATIONAL CLEAVAGE.
[8]"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
[9]"SOM1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae."
Esser K., Pratje E., Michaelis G.
Mol. Gen. Genet. 252:437-445(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: POSTRANSLATIONAL CLEAVAGE BY IMP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02200 Genomic DNA. Translation: AAA32158.2.
V00706 Genomic DNA. Translation: CAA24078.1.
V00685 Genomic DNA. Translation: CAA24056.1.
AJ011856 Genomic DNA. Translation: CAA09845.1.
J05007 Genomic DNA. Translation: AAA32155.2.
L36888 Genomic DNA. Translation: AAA67528.1.
D55725 Genomic DNA. Translation: BAA09539.1.
PIROBBY2. A00478.
RefSeqNP_009326.1. NM_001184373.1.

3D structure databases

ProteinModelPortalP00410.
SMRP00410. Positions 20-247.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34812. 35 interactions.
DIPDIP-7592N.
IntActP00410. 9 interactions.
MINTMINT-434441.

Protein family/group databases

TCDB3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBP00410.
PaxDbP00410.
PeptideAtlasP00410.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiQ0250; Q0250; Q0250.
GeneID854622.
KEGGsce:Q0250.

Organism-specific databases

CYGDQ0250.
SGDS000007281. COX2.

Phylogenomic databases

eggNOGCOG1622.
GeneTreeENSGT00390000017410.
HOGENOMHOG000264988.
KOK02261.
OMANHEMISS.
OrthoDBEOG7WDNDC.

Enzyme and pathway databases

BioCycMetaCyc:Q0250-MONOMER.
YEAST:Q0250-MONOMER.

Gene expression databases

GenevestigatorP00410.

Family and domain databases

Gene3D1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsTIGR02866. CoxB. 1 hit.
PROSITEPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977130.
PROP00410.

Entry information

Entry nameCOX2_YEAST
AccessionPrimary (citable) accession number: P00410
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families