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P00410

- COX2_YEAST

UniProt

P00410 - COX2_YEAST

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Protein
Cytochrome c oxidase subunit 2
Gene
COX2, OXI1, Q0250
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

Copper A.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 162Cleavage; by IMP1
Metal bindingi186 – 1861Copper A Inferred
Metal bindingi221 – 2211Copper A Inferred
Metal bindingi225 – 2251Copper A Inferred
Metal bindingi229 – 2291Copper A Inferred

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. cytochrome-c oxidase activity Source: UniProtKB-EC
  3. protein binding Source: IntAct

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
  3. mitochondrial electron transport, cytochrome c to oxygen Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:Q0250-MONOMER.
YEAST:Q0250-MONOMER.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide II
Gene namesi
Name:COX2
Synonyms:OXI1
Ordered Locus Names:Q0250
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Mitochondrion

Organism-specific databases

CYGDiQ0250.
SGDiS000007281. COX2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 4227Mitochondrial intermembrane Reviewed prediction
Add
BLAST
Transmembranei43 – 6422Helical; Reviewed prediction
Add
BLAST
Topological domaini65 – 8218Mitochondrial matrix Reviewed prediction
Add
BLAST
Transmembranei83 – 10725Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 251144Mitochondrial intermembrane Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial respiratory chain complex IV Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1515Removed in mature form
PRO_0000419180Add
BLAST
Chaini16 – 251236Cytochrome c oxidase subunit 2
PRO_0000006048Add
BLAST

Post-translational modificationi

The N-terminal sequence of COX2 is processed by IMP1.

Proteomic databases

MaxQBiP00410.
PaxDbiP00410.
PeptideAtlasiP00410.

Expressioni

Gene expression databases

GenevestigatoriP00410.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Binary interactionsi

WithEntry#Exp.IntActNotes
COX20Q049353EBI-5024,EBI-36910

Protein-protein interaction databases

BioGridi34812. 35 interactions.
DIPiDIP-7592N.
IntActiP00410. 9 interactions.
MINTiMINT-434441.

Structurei

3D structure databases

ProteinModelPortaliP00410.
SMRiP00410. Positions 20-247.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1622.
GeneTreeiENSGT00390000017410.
HOGENOMiHOG000264988.
KOiK02261.
OMAiNHEMISS.
OrthoDBiEOG7WDNDC.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00410-1 [UniParc]FASTAAdd to Basket

« Hide

MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI    50
LGLVSWMLYT IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS 100
FILLYLCDEV ISPAMTIKAI GYQWYWKYEY SDFINDSGET VEFESYVIPD 150
ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT AADVIHDFAI PSLGIKVDAT 200
PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS LPKFLEWLNE 250
Q 251
Length:251
Mass (Da):28,567
Last modified:July 21, 1986 - v1
Checksum:iBA5717C37330DF41
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591Y → N in AAA32155. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02200 Genomic DNA. Translation: AAA32158.2.
V00706 Genomic DNA. Translation: CAA24078.1.
V00685 Genomic DNA. Translation: CAA24056.1.
AJ011856 Genomic DNA. Translation: CAA09845.1.
J05007 Genomic DNA. Translation: AAA32155.2.
L36888 Genomic DNA. Translation: AAA67528.1.
D55725 Genomic DNA. Translation: BAA09539.1.
PIRiA00478. OBBY2.
RefSeqiNP_009326.1. NM_001184373.1.

Genome annotation databases

EnsemblFungiiQ0250; Q0250; Q0250.
GeneIDi854622.
KEGGisce:Q0250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02200 Genomic DNA. Translation: AAA32158.2 .
V00706 Genomic DNA. Translation: CAA24078.1 .
V00685 Genomic DNA. Translation: CAA24056.1 .
AJ011856 Genomic DNA. Translation: CAA09845.1 .
J05007 Genomic DNA. Translation: AAA32155.2 .
L36888 Genomic DNA. Translation: AAA67528.1 .
D55725 Genomic DNA. Translation: BAA09539.1 .
PIRi A00478. OBBY2.
RefSeqi NP_009326.1. NM_001184373.1.

3D structure databases

ProteinModelPortali P00410.
SMRi P00410. Positions 20-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34812. 35 interactions.
DIPi DIP-7592N.
IntActi P00410. 9 interactions.
MINTi MINT-434441.

Protein family/group databases

TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBi P00410.
PaxDbi P00410.
PeptideAtlasi P00410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii Q0250 ; Q0250 ; Q0250 .
GeneIDi 854622.
KEGGi sce:Q0250.

Organism-specific databases

CYGDi Q0250.
SGDi S000007281. COX2.

Phylogenomic databases

eggNOGi COG1622.
GeneTreei ENSGT00390000017410.
HOGENOMi HOG000264988.
KOi K02261.
OMAi NHEMISS.
OrthoDBi EOG7WDNDC.

Enzyme and pathway databases

BioCyci MetaCyc:Q0250-MONOMER.
YEAST:Q0250-MONOMER.

Miscellaneous databases

NextBioi 977130.
PROi P00410.

Gene expression databases

Genevestigatori P00410.

Family and domain databases

Gene3Di 1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProi IPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view ]
Pfami PF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view ]
SUPFAMi SSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsi TIGR02866. CoxB. 1 hit.
PROSITEi PS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase."
    Coruzzi G., Tzagoloff A.
    J. Biol. Chem. 254:9324-9330(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS200/A1.
  2. "Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II."
    Fox T.D.
    Proc. Natl. Acad. Sci. U.S.A. 76:6534-6538(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  3. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
    Foury F., Roganti T., Lecrenier N., Purnelle B.
    FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2."
    Cameron V.L., Fox T.D., Poyton R.O.
    J. Biol. Chem. 264:13391-13394(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
  5. "Use of the UGA terminator as a tryptophan codon in yeast mitochondria."
    Macino G., Coruzzi G., Nobrega F.G., Li M., Tzagoloff A.
    Proc. Natl. Acad. Sci. U.S.A. 76:3784-3785(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 121-132.
  6. Nakagawa Y.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-200.
    Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
  7. "A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae."
    Pratje E., Mannhaupt G., Michaelis G., Beyreuther K.
    EMBO J. 2:1049-1054(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSTRANSLATIONAL CLEAVAGE.
  8. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
  9. "SOM1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae."
    Esser K., Pratje E., Michaelis G.
    Mol. Gen. Genet. 252:437-445(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSTRANSLATIONAL CLEAVAGE BY IMP1.

Entry informationi

Entry nameiCOX2_YEAST
AccessioniPrimary (citable) accession number: P00410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3

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