Cytochrome c oxidase subunit 2 precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P00410 (COX2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 118. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 2
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide II

Gene names

Name:	COX2
Synonyms:	OXI1
Ordered Locus Names:	Q0250

Encoded on

Mitochondrion

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Catalytic activity	4 ferrocytochrome c + O₂ + 4 H⁺ = 4 ferricytochrome c + 2 H₂O.
Cofactor	Copper A.
Subunit structure	Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Post-translational modification	The signal sequence of COX2 is processed by IMP1.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype. Source: SGD mitochondrial electron transport, cytochrome c to oxygen Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex IV Inferred from physical interaction. Source: SGD
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COX20	Q04935	3	EBI-5024,EBI-36910

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

Chain

16 – 251

236

Cytochrome c oxidase subunit 2

PRO_0000006048

Regions

Topological domain

16 – 42

Mitochondrial intermembrane Potential

Transmembrane

43 – 64

Helical; Potential

Topological domain

65 – 82

Mitochondrial matrix Potential

Transmembrane

83 – 107

Helical; Potential

Topological domain

108 – 251

144

Mitochondrial intermembrane Potential

Sites

Metal binding

186

Copper A Probable

Metal binding

221

Copper A Probable

Metal binding

225

Copper A Probable

Metal binding

229

Copper A Probable

Experimental info

Sequence conflict

Y → N in AAA32155. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P00410 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: BA5717C37330DF41
Show »

FASTA

251

28,567

        10         20         30         40         50         60 
MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI LGLVSWMLYT 

        70         80         90        100        110        120 
IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS FILLYLCDEV ISPAMTIKAI 

       130        140        150        160        170        180 
GYQWYWKYEY SDFINDSGET VEFESYVIPD ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT 

       190        200        210        220        230        240 
AADVIHDFAI PSLGIKVDAT PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS 

       250 
LPKFLEWLNE Q

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase." Coruzzi G., Tzagoloff A. J. Biol. Chem. 254:9324-9330(1979) [PubMed: 225327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DS200/A1.
[2]	"Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II." Fox T.D. Proc. Natl. Acad. Sci. U.S.A. 76:6534-6538(1979) [PubMed: 230513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24657 / D273-10B.
[3]	"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae." Foury F., Roganti T., Lecrenier N., Purnelle B. FEBS Lett. 440:325-331(1998) [PubMed: 9872396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2." Cameron V.L., Fox T.D., Poyton R.O. J. Biol. Chem. 264:13391-13394(1989) [PubMed: 2547760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
[5]	"Use of the UGA terminator as a tryptophan codon in yeast mitochondria." Macino G., Coruzzi G., Nobrega F.G., Li M., Tzagoloff A. Proc. Natl. Acad. Sci. U.S.A. 76:3784-3785(1979) [PubMed: 226981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 121-132.
[6]	Nakagawa Y. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-200. Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
[7]	"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method." Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G. Eur. J. Biochem. 227:296-302(1995) [PubMed: 7851399] [Abstract] Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02200 Genomic DNA. Translation: AAA32158.2. V00706 Genomic DNA. Translation: CAA24078.1. V00685 Genomic DNA. Translation: CAA24056.1. AJ011856 Genomic DNA. Translation: CAA09845.1. J05007 Genomic DNA. Translation: AAA32155.2. L36888 Genomic DNA. Translation: AAA67528.1. D55725 Genomic DNA. Translation: BAA09539.1.
PIR	OBBY2. A00478.
RefSeq	NP_009326.1. NM_001184373.1.
3D structure databases
ProteinModelPortal	P00410.
SMR	P00410. Positions 17-251.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7592N.
IntAct	P00410. 3 interactions.
MINT	MINT-434441.
STRING	P00410.
Protein family/group databases
TCDB	3.D.4.8.1. proton-translocating cytochrome oxidase (COX) superfamily.
Proteomic databases
PeptideAtlas	P00410.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	Q0250; Q0250; Q0250.
GeneID	854622.
KEGG	sce:Q0250.
NMPDR	fig\|4932.3.peg.1878.
Organism-specific databases
CYGD	Q0250.
SGD	S000007281. COX2.
Phylogenomic databases
HOGENOM	HBG390534.
OrthoDB	EOG4ZW8M5.
Gene expression databases
ArrayExpress	P00410.
Genevestigator	P00410.
GermOnline	Q0250. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR014222. Cyt_c_oxidase_su2. IPR015964. Cyt_c_oxidase_su2-like_TM_dom. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.90. COX2_TM. 1 hit. G3DSA:2.60.40.420. Cupredoxin. 1 hit.
KO	K02261.
Pfam	PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
PRINTS	PR01166. CYCOXIDASEII.
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
TIGRFAMs	TIGR02866. CoxB. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977130.

Entry information

Entry name

COX2_YEAST

Accession

Primary (citable) accession number: P00410

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	December 14, 2011
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents