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Protein

Cytochrome c oxidase subunit 2

Gene

COX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

Cu cationNote: Binds a copper A center.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 162Cleavage; by IMP1
Metal bindingi186 – 1861Copper ACurated
Metal bindingi221 – 2211Copper ACurated
Metal bindingi225 – 2251Copper ACurated
Metal bindingi229 – 2291Copper ACurated

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: SGD
  • hydrogen ion transmembrane transport Source: GOC
  • mitochondrial electron transport, cytochrome c to oxygen Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:Q0250-MONOMER.
YEAST:Q0250-MONOMER.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 2 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide II
Gene namesi
Name:COX2
Synonyms:OXI1
Ordered Locus Names:Q0250
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Mitochondrion

Organism-specific databases

CYGDiQ0250.
EuPathDBiFungiDB:Q0250.
SGDiS000007281. COX2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 4227Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei43 – 6422HelicalSequence AnalysisAdd
BLAST
Topological domaini65 – 8218Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei83 – 10725HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 251144Mitochondrial intermembraneSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial respiratory chain complex IV Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1515Removed in mature formPRO_0000419180Add
BLAST
Chaini16 – 251236Cytochrome c oxidase subunit 2PRO_0000006048Add
BLAST

Post-translational modificationi

The N-terminal sequence of COX2 is processed by IMP1.

Proteomic databases

MaxQBiP00410.
PaxDbiP00410.
PeptideAtlasiP00410.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Binary interactionsi

WithEntry#Exp.IntActNotes
COX20Q049353EBI-5024,EBI-36910

Protein-protein interaction databases

BioGridi34812. 37 interactions.
DIPiDIP-7592N.
IntActiP00410. 9 interactions.
MINTiMINT-434441.
STRINGi4932.Q0250.

Structurei

3D structure databases

ProteinModelPortaliP00410.
SMRiP00410. Positions 20-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1622.
GeneTreeiENSGT00390000017410.
HOGENOMiHOG000264988.
InParanoidiP00410.
KOiK02261.
OMAiGEHIEGN.
OrthoDBiEOG7WDNDC.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00410-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDLLRLQLT TFIMNDVPTP YACYFQDSAT PNQEGILELH DNIMFYLLVI
60 70 80 90 100
LGLVSWMLYT IVMTYSKNPI AYKYIKHGQT IEVIWTIFPA VILLIIAFPS
110 120 130 140 150
FILLYLCDEV ISPAMTIKAI GYQWYWKYEY SDFINDSGET VEFESYVIPD
160 170 180 190 200
ELLEEGQLRL LDTDTSMVVP VDTHIRFVVT AADVIHDFAI PSLGIKVDAT
210 220 230 240 250
PGRLNQVSAL IQREGVFYGA CSELCGTGHA NMPIKIEAVS LPKFLEWLNE

Q
Length:251
Mass (Da):28,567
Last modified:July 21, 1986 - v1
Checksum:iBA5717C37330DF41
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591Y → N in AAA32155 (PubMed:2547760).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02200 Genomic DNA. Translation: AAA32158.2.
V00706 Genomic DNA. Translation: CAA24078.1.
V00685 Genomic DNA. Translation: CAA24056.1.
AJ011856 Genomic DNA. Translation: CAA09845.1.
J05007 Genomic DNA. Translation: AAA32155.2.
L36888 Genomic DNA. Translation: AAA67528.1.
D55725 Genomic DNA. Translation: BAA09539.1.
PIRiA00478. OBBY2.
RefSeqiNP_009326.1. NC_001224.1.

Genome annotation databases

EnsemblFungiiQ0250; Q0250; Q0250.
GeneIDi854622.
KEGGisce:Q0250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02200 Genomic DNA. Translation: AAA32158.2.
V00706 Genomic DNA. Translation: CAA24078.1.
V00685 Genomic DNA. Translation: CAA24056.1.
AJ011856 Genomic DNA. Translation: CAA09845.1.
J05007 Genomic DNA. Translation: AAA32155.2.
L36888 Genomic DNA. Translation: AAA67528.1.
D55725 Genomic DNA. Translation: BAA09539.1.
PIRiA00478. OBBY2.
RefSeqiNP_009326.1. NC_001224.1.

3D structure databases

ProteinModelPortaliP00410.
SMRiP00410. Positions 20-248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34812. 37 interactions.
DIPiDIP-7592N.
IntActiP00410. 9 interactions.
MINTiMINT-434441.
STRINGi4932.Q0250.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBiP00410.
PaxDbiP00410.
PeptideAtlasiP00410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiQ0250; Q0250; Q0250.
GeneIDi854622.
KEGGisce:Q0250.

Organism-specific databases

CYGDiQ0250.
EuPathDBiFungiDB:Q0250.
SGDiS000007281. COX2.

Phylogenomic databases

eggNOGiCOG1622.
GeneTreeiENSGT00390000017410.
HOGENOMiHOG000264988.
InParanoidiP00410.
KOiK02261.
OMAiGEHIEGN.
OrthoDBiEOG7WDNDC.

Enzyme and pathway databases

BioCyciMetaCyc:Q0250-MONOMER.
YEAST:Q0250-MONOMER.

Miscellaneous databases

NextBioi977130.
PROiP00410.

Family and domain databases

Gene3Di1.10.287.90. 1 hit.
2.60.40.420. 1 hit.
InterProiIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PfamiPF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsiTIGR02866. CoxB. 1 hit.
PROSITEiPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Assembly of the mitochondrial membrane system. DNA sequence of subunit 2 of yeast cytochrome oxidase."
    Coruzzi G., Tzagoloff A.
    J. Biol. Chem. 254:9324-9330(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DS200/A1.
  2. "Five TGA 'stop' codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II."
    Fox T.D.
    Proc. Natl. Acad. Sci. U.S.A. 76:6534-6538(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  3. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
    Foury F., Roganti T., Lecrenier N., Purnelle B.
    FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Isolation and characterization of a yeast strain carrying a mutation in the mitochondrial promoter for COX2."
    Cameron V.L., Fox T.D., Poyton R.O.
    J. Biol. Chem. 264:13391-13394(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
  5. "Use of the UGA terminator as a tryptophan codon in yeast mitochondria."
    Macino G., Coruzzi G., Nobrega F.G., Li M., Tzagoloff A.
    Proc. Natl. Acad. Sci. U.S.A. 76:3784-3785(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 121-132.
  6. Nakagawa Y.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-200.
    Strain: ATCC 18824 / CBS 1171 / DSM 70449 / IFO 10217 / NRRL Y-12632.
  7. "A nuclear mutation prevents processing of a mitochondrially encoded membrane protein in Saccharomyces cerevisiae."
    Pratje E., Mannhaupt G., Michaelis G., Beyreuther K.
    EMBO J. 2:1049-1054(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSTRANSLATIONAL CLEAVAGE.
  8. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
  9. "SOM1, a small new gene required for mitochondrial inner membrane peptidase function in Saccharomyces cerevisiae."
    Esser K., Pratje E., Michaelis G.
    Mol. Gen. Genet. 252:437-445(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSTRANSLATIONAL CLEAVAGE BY IMP1.

Entry informationi

Entry nameiCOX2_YEAST
AccessioniPrimary (citable) accession number: P00410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 24, 2015
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.