P00405 (COX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 2

Alternative name(s):
Cytochrome c oxidase polypeptide II

Gene names

Name:	Mtco2
Synonyms:	COII, mt-Co2

Encoded on

Mitochondrion

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Cofactor	Copper A.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 18614015PubMed 19837698. Source: MGI respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	Cytochrome c oxidase subunit 2		PRO_0000183635
Regions
	Topological domain	1 – 26	26	Mitochondrial intermembrane Potential
	Transmembrane	27 – 48	22	Helical; Potential
	Topological domain	49 – 62	14	Mitochondrial matrix Potential
	Transmembrane	63 – 82	20	Helical; Potential
	Topological domain	83 – 227	145	Mitochondrial intermembrane Potential
Sites
	Metal binding	161	1	Copper A Probable
	Metal binding	196	1	Copper A Probable
	Metal binding	200	1	Copper A Probable
	Metal binding	204	1	Copper A Probable

Sequences

	Sequence		Length	Mass (Da)
	P00405 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 306C6EAD54802D11	FASTA	227	25,976
	10 20 30 40 50 60 MAYPFQLGLQ DATSPIMEEL MNFHDHTLMI VFLISSLVLY IISLMLTTKL THTSTMDAQE 70 80 90 100 110 120 VETIWTILPA VILIMIALPS LRILYMMDEI NNPVLTVKTM GHQWYWSYEY TDYEDLCFDS 130 140 150 160 170 180 YMIPTNDLKP GELRLLEVDN RVVLPMELPI RMLISSEDVL HSWAVPSLGL KTDAIPGRLN 190 200 210 220 QATVTSNRPG LFYGQCSEIC GSNHSFMPIV LEMVPLKYFE NWSASMI

References

[1]	"Sequence and gene organization of mouse mitochondrial DNA." Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A. Cell 26:167-180(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a deleted mitochondrial DNA molecule in heteroplasmic mice." Nelson I., Gerasimov S., Marsac C., Lestienne P., Boursot P. Mamm. Genome 4:680-683(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-63.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 83-98; 135-171 AND 218-227, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01420 Genomic DNA. Translation: AAB48647.1. V00711 Genomic DNA. Translation: CAA24083.1. S68119 Genomic DNA. Translation: AAP15814.1.
IPI	IPI00131176.
PIR	OBMS2. A00474.
RefSeq	NP_904331.1. NC_005089.1.
3D structure databases
ProteinModelPortal	P00405.
SMR	P00405. Positions 2-227.
ModBase	Search...
Protein-protein interaction databases
IntAct	P00405. 2 interactions.
MINT	MINT-1858197.
STRING	10090.ENSMUSP00000080994.
PTM databases
PhosphoSite	P00405.
2D gel databases
SWISS-2DPAGE	P00405.
Proteomic databases
PaxDb	P00405.
PRIDE	P00405.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000082405; ENSMUSP00000080994; ENSMUSG00000064354.
GeneID	17709.
KEGG	mmu:17709.
Organism-specific databases
CTD	4513.
MGI	MGI:102503. mt-Co2.
Phylogenomic databases
eggNOG	COG1622.
HOGENOM	HOG000264988.
HOVERGEN	HBG012727.
InParanoid	P00405.
KO	K02261.
OrthoDB	EOG4BZN3N.
ProtClustDB	MTH00098.
Gene expression databases
ArrayExpress	P00405.
Bgee	P00405.
Genevestigator	P00405.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR014222. Cyt_c_oxidase_su2. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Pfam	PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
TIGRFAMs	TIGR02866. CoxB. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292315.
SOURCE	Search...

Entry information

Entry name

COX2_MOUSE

Accession

Primary (citable) accession number: P00405
Secondary accession number(s): Q85K21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 29, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program