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P00401

- COX1_YEAST

UniProt

P00401 - COX1_YEAST

Protein

Cytochrome c oxidase subunit 1

Gene

COX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (23 Apr 2003)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Iron (heme A axial ligand)Curated
    Metal bindingi241 – 2411Copper BCurated
    Metal bindingi245 – 2451Copper BCurated
    Metal bindingi290 – 2901Copper BCurated
    Metal bindingi291 – 2911Copper BCurated
    Metal bindingi376 – 3761Iron (heme A3 axial ligand)Curated
    Metal bindingi378 – 3781Iron (heme A axial ligand)Curated

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: SGD
    2. hydrogen ion transmembrane transport Source: GOC
    3. mitochondrial electron transport, cytochrome c to oxygen Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:Q0045-MONOMER.
    YEAST:Q0045-MONOMER.
    UniPathwayiUPA00705.

    Protein family/group databases

    TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:COX1
    Synonyms:OXI3
    Ordered Locus Names:Q0045
    Encoded oniMitochondrion
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Mitochondrion

    Organism-specific databases

    CYGDiQ0045.
    SGDiS000007260. COX1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial respiratory chain complex IV Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534Cytochrome c oxidase subunit 1PRO_0000183432Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki241 ↔ 2451'-histidyl-3'-tyrosine (His-Tyr)By similarity

    Proteomic databases

    MaxQBiP00401.
    PeptideAtlasiP00401.

    Expressioni

    Gene expression databases

    GenevestigatoriP00401.

    Interactioni

    Subunit structurei

    Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

    Protein-protein interaction databases

    BioGridi34790. 44 interactions.
    IntActiP00401. 6 interactions.
    MINTiMINT-4981480.

    Structurei

    3D structure databases

    ProteinModelPortaliP00401.
    SMRiP00401. Positions 2-530.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei58 – 8023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei101 – 12323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 17023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei183 – 20523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei337 – 35923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei374 – 39623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei409 – 43123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47323HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0843.
    GeneTreeiENSGT00390000001518.
    HOGENOMiHOG000085274.
    KOiK02256.
    OMAiHFGVMFT.
    OrthoDBiEOG72C58S.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG    50
    NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN 100
    IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA 150
    LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL 200
    PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG HPEVYILIIP 250
    GFGIISHVVS TYSKKPVFGE ISMVYAMASI GLLGFLVWSH HMYIVGLDAD 300
    TRAYFTSATM IIAIPTGIKI FSWLATIHGG SIRLATPMLY AIAFLFLFTM 350
    GGLTGVALAN ASLDVAFHDT YYVVGHFHYV LSMGAIFSLF AGYYYWSPQI 400
    LGLNYNEKLA QIQFWLIFIG ANVIFFPMHF LGINGMPRRI PDYPDAFAGW 450
    NYVASIGSFI ATLSLFLFIY ILYDQLVNGL NNKVNNKSVI YNKAPDFVES 500
    NTIFNLNTVK SSSIEFLLTS PPAVHSFNTP AVQS 534
    Length:534
    Mass (Da):58,798
    Last modified:April 23, 2003 - v2
    Checksum:i5D555084C127E8B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571Missing in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti69 – 691F → CT in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti224 – 2241S → A in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti328 – 3281H → Y in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti378 – 3781H → Y in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti479 – 4857Missing in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti492 – 4921N → A in CAA24070. (PubMed:6254986)Curated
    Sequence conflicti496 – 51015Missing in CAA24070. (PubMed:6254986)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00694 Genomic DNA. Translation: CAA24070.1.
    AJ011856 Genomic DNA. Translation: CAA09824.1.
    X14910 Genomic DNA. Translation: CAA33036.1.
    PIRiA00468. ODBY1.
    S78640.
    RefSeqiNP_009305.1. NM_001184352.1.

    Genome annotation databases

    EnsemblFungiiQ0045; Q0045; Q0045.
    GeneIDi854598.
    KEGGisce:Q0045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00694 Genomic DNA. Translation: CAA24070.1 .
    AJ011856 Genomic DNA. Translation: CAA09824.1 .
    X14910 Genomic DNA. Translation: CAA33036.1 .
    PIRi A00468. ODBY1.
    S78640.
    RefSeqi NP_009305.1. NM_001184352.1.

    3D structure databases

    ProteinModelPortali P00401.
    SMRi P00401. Positions 2-530.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34790. 44 interactions.
    IntActi P00401. 6 interactions.
    MINTi MINT-4981480.

    Protein family/group databases

    TCDBi 3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Proteomic databases

    MaxQBi P00401.
    PeptideAtlasi P00401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii Q0045 ; Q0045 ; Q0045 .
    GeneIDi 854598.
    KEGGi sce:Q0045.

    Organism-specific databases

    CYGDi Q0045.
    SGDi S000007260. COX1.

    Phylogenomic databases

    eggNOGi COG0843.
    GeneTreei ENSGT00390000001518.
    HOGENOMi HOG000085274.
    KOi K02256.
    OMAi HFGVMFT.
    OrthoDBi EOG72C58S.

    Enzyme and pathway databases

    UniPathwayi UPA00705 .
    BioCyci MetaCyc:Q0045-MONOMER.
    YEAST:Q0045-MONOMER.

    Miscellaneous databases

    NextBioi 977080.
    PROi P00401.

    Gene expression databases

    Genevestigatori P00401.

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrome oxidase."
      Bonitz S.G., Coruzzi G., Thalenfeld B.E., Tzagoloff A., Macino G.
      J. Biol. Chem. 255:11927-11941(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 24657 / D273-10B.
    2. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
      Foury F., Roganti T., Lecrenier N., Purnelle B.
      FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "Sequence of the yeast mitochondrial OX13/OL12 promoter region."
      Seraphin B., Simon M., Jacq C., Faye G.
      Nucleic Acids Res. 17:4886-4886(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
      Strain: ATCC 24657 / D273-10B.
    4. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
      Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
      Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.

    Entry informationi

    Entry nameiCOX1_YEAST
    AccessioniPrimary (citable) accession number: P00401
    Secondary accession number(s): Q9ZZX6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 23, 2003
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

    External Data

    Dasty 3