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P00401 (COX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase subunit 1

EC=1.9.3.1
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene names
Name:COX1
Synonyms:OXI3
Ordered Locus Names:Q0045
Encoded onMitochondrion
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathway

Energy metabolism; oxidative phosphorylation.

Subunit structure

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the heme-copper respiratory oxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Cytochrome c oxidase subunit 1
PRO_0000183432

Regions

Transmembrane16 – 3823Helical; Potential
Transmembrane58 – 8023Helical; Potential
Transmembrane101 – 12323Helical; Potential
Transmembrane148 – 17023Helical; Potential
Transmembrane183 – 20523Helical; Potential
Transmembrane237 – 25923Helical; Potential
Transmembrane266 – 28823Helical; Potential
Transmembrane303 – 32523Helical; Potential
Transmembrane337 – 35923Helical; Potential
Transmembrane374 – 39623Helical; Potential
Transmembrane409 – 43123Helical; Potential
Transmembrane451 – 47323Helical; Potential

Sites

Metal binding621Iron (heme A axial ligand) Probable
Metal binding2411Copper B Probable
Metal binding2451Copper B Probable
Metal binding2901Copper B Probable
Metal binding2911Copper B Probable
Metal binding3761Iron (heme A3 axial ligand) Probable
Metal binding3781Iron (heme A axial ligand) Probable

Amino acid modifications

Cross-link241 ↔ 2451'-histidyl-3'-tyrosine (His-Tyr) By similarity

Experimental info

Sequence conflict571Missing in CAA24070. Ref.1
Sequence conflict691F → CT in CAA24070. Ref.1
Sequence conflict2241S → A in CAA24070. Ref.1
Sequence conflict3281H → Y in CAA24070. Ref.1
Sequence conflict3781H → Y in CAA24070. Ref.1
Sequence conflict479 – 4857Missing in CAA24070. Ref.1
Sequence conflict4921N → A in CAA24070. Ref.1
Sequence conflict496 – 51015Missing in CAA24070. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P00401 [UniParc].

Last modified April 23, 2003. Version 2.
Checksum: 5D555084C127E8B1

FASTA53458,798
        10         20         30         40         50         60 
MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG NSQLFNVLVV 

        70         80         90        100        110        120 
GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN IAFWVLPMGL VCLVTSTLVE 

       130        140        150        160        170        180 
SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA LHLTSISSLL GAINFIVTTL NMRTNGMTMH 

       190        200        210        220        230        240 
KLPLFVWSIF ITAFLLLLSL PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG 

       250        260        270        280        290        300 
HPEVYILIIP GFGIISHVVS TYSKKPVFGE ISMVYAMASI GLLGFLVWSH HMYIVGLDAD 

       310        320        330        340        350        360 
TRAYFTSATM IIAIPTGIKI FSWLATIHGG SIRLATPMLY AIAFLFLFTM GGLTGVALAN 

       370        380        390        400        410        420 
ASLDVAFHDT YYVVGHFHYV LSMGAIFSLF AGYYYWSPQI LGLNYNEKLA QIQFWLIFIG 

       430        440        450        460        470        480 
ANVIFFPMHF LGINGMPRRI PDYPDAFAGW NYVASIGSFI ATLSLFLFIY ILYDQLVNGL 

       490        500        510        520        530 
NNKVNNKSVI YNKAPDFVES NTIFNLNTVK SSSIEFLLTS PPAVHSFNTP AVQS 

« Hide

References

« Hide 'large scale' references
[1]"Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrome oxidase."
Bonitz S.G., Coruzzi G., Thalenfeld B.E., Tzagoloff A., Macino G.
J. Biol. Chem. 255:11927-11941(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24657 / D273-10B.
[2]"The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
Foury F., Roganti T., Lecrenier N., Purnelle B.
FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Sequence of the yeast mitochondrial OX13/OL12 promoter region."
Seraphin B., Simon M., Jacq C., Faye G.
Nucleic Acids Res. 17:4886-4886(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
Strain: ATCC 24657 / D273-10B.
[4]"Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00694 Genomic DNA. Translation: CAA24070.1.
AJ011856 Genomic DNA. Translation: CAA09824.1.
X14910 Genomic DNA. Translation: CAA33036.1.
PIRODBY1. A00468.
S78640.
RefSeqNP_009305.1. NM_001184352.1.

3D structure databases

ProteinModelPortalP00401.
SMRP00401. Positions 2-530.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34790. 44 interactions.
IntActP00401. 6 interactions.
MINTMINT-4981480.

Protein family/group databases

TCDB3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

PeptideAtlasP00401.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiQ0045; Q0045; Q0045.
GeneID854598.
KEGGsce:Q0045.

Organism-specific databases

CYGDQ0045.
SGDS000007260. COX1.

Phylogenomic databases

eggNOGCOG0843.
GeneTreeENSGT00390000001518.
HOGENOMHOG000085274.
KOK02256.
OMAVNGAFGN.
OrthoDBEOG72C58S.

Enzyme and pathway databases

BioCycMetaCyc:Q0045-MONOMER.
YEAST:Q0045-MONOMER.
UniPathwayUPA00705.

Gene expression databases

GenevestigatorP00401.

Family and domain databases

Gene3D1.20.210.10. 1 hit.
InterProIPR000883. Cyt_c_Oxase_su1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERPTHR10422. PTHR10422. 1 hit.
PfamPF00115. COX1. 1 hit.
[Graphical view]
PRINTSPR01165. CYCOXIDASEI.
SUPFAMSSF81442. SSF81442. 1 hit.
PROSITEPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977080.
PROP00401.

Entry information

Entry nameCOX1_YEAST
AccessionPrimary (citable) accession number: P00401
Secondary accession number(s): Q9ZZX6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 23, 2003
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways