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Protein

Cytochrome c oxidase subunit 1

Gene

COX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron (heme A axial ligand)Curated
Metal bindingi241 – 2411Copper BCurated
Metal bindingi245 – 2451Copper BCurated
Metal bindingi290 – 2901Copper BCurated
Metal bindingi291 – 2911Copper BCurated
Metal bindingi376 – 3761Iron (heme A3 axial ligand)Curated
Metal bindingi378 – 3781Iron (heme A axial ligand)Curated

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. hydrogen ion transmembrane transport Source: GOC
  3. mitochondrial electron transport, cytochrome c to oxygen Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:Q0045-MONOMER.
YEAST:Q0045-MONOMER.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:COX1
Synonyms:OXI3
Ordered Locus Names:Q0045
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Mitochondrion

Organism-specific databases

CYGDiQ0045.
EuPathDBiFungiDB:Q0045.
SGDiS000007260. COX1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3823HelicalSequence AnalysisAdd
BLAST
Transmembranei58 – 8023HelicalSequence AnalysisAdd
BLAST
Transmembranei101 – 12323HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 17023HelicalSequence AnalysisAdd
BLAST
Transmembranei183 – 20523HelicalSequence AnalysisAdd
BLAST
Transmembranei237 – 25923HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28823HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32523HelicalSequence AnalysisAdd
BLAST
Transmembranei337 – 35923HelicalSequence AnalysisAdd
BLAST
Transmembranei374 – 39623HelicalSequence AnalysisAdd
BLAST
Transmembranei409 – 43123HelicalSequence AnalysisAdd
BLAST
Transmembranei451 – 47323HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial respiratory chain complex IV Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534Cytochrome c oxidase subunit 1PRO_0000183432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki241 ↔ 2451'-histidyl-3'-tyrosine (His-Tyr)By similarity

Proteomic databases

MaxQBiP00401.
PeptideAtlasiP00401.

Expressioni

Gene expression databases

GenevestigatoriP00401.

Interactioni

Subunit structurei

Cytochrome c oxidase is composed of 11 subunits: one copy each of COX1, COX2, COX3, COX4, COX5A or COX5B, COX6, COX7, COX9, COX12, COX13 and two copies of COX8.

Protein-protein interaction databases

BioGridi34790. 44 interactions.
IntActiP00401. 6 interactions.
MINTiMINT-4981480.

Structurei

3D structure databases

ProteinModelPortaliP00401.
SMRiP00401. Positions 2-530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
GeneTreeiENSGT00390000001518.
HOGENOMiHOG000085274.
InParanoidiP00401.
KOiK02256.
OMAiAALCTNV.
OrthoDBiEOG72C58S.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQRWLYSTN AKDIAVLYFM LAIFSGMAGT AMSLIIRLEL AAPGSQYLHG
60 70 80 90 100
NSQLFNVLVV GHAVLMIFFL VMPALIGGFG NYLLPLMIGA TDTAFPRINN
110 120 130 140 150
IAFWVLPMGL VCLVTSTLVE SGAGTGWTVY PPLSSIQAHS GPSVDLAIFA
160 170 180 190 200
LHLTSISSLL GAINFIVTTL NMRTNGMTMH KLPLFVWSIF ITAFLLLLSL
210 220 230 240 250
PVLSAGITML LLDRNFNTSF FEVSGGGDPI LYEHLFWFFG HPEVYILIIP
260 270 280 290 300
GFGIISHVVS TYSKKPVFGE ISMVYAMASI GLLGFLVWSH HMYIVGLDAD
310 320 330 340 350
TRAYFTSATM IIAIPTGIKI FSWLATIHGG SIRLATPMLY AIAFLFLFTM
360 370 380 390 400
GGLTGVALAN ASLDVAFHDT YYVVGHFHYV LSMGAIFSLF AGYYYWSPQI
410 420 430 440 450
LGLNYNEKLA QIQFWLIFIG ANVIFFPMHF LGINGMPRRI PDYPDAFAGW
460 470 480 490 500
NYVASIGSFI ATLSLFLFIY ILYDQLVNGL NNKVNNKSVI YNKAPDFVES
510 520 530
NTIFNLNTVK SSSIEFLLTS PPAVHSFNTP AVQS
Length:534
Mass (Da):58,798
Last modified:April 23, 2003 - v2
Checksum:i5D555084C127E8B1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571Missing in CAA24070 (PubMed:6254986).Curated
Sequence conflicti69 – 691F → CT in CAA24070 (PubMed:6254986).Curated
Sequence conflicti224 – 2241S → A in CAA24070 (PubMed:6254986).Curated
Sequence conflicti328 – 3281H → Y in CAA24070 (PubMed:6254986).Curated
Sequence conflicti378 – 3781H → Y in CAA24070 (PubMed:6254986).Curated
Sequence conflicti479 – 4857Missing in CAA24070 (PubMed:6254986).Curated
Sequence conflicti492 – 4921N → A in CAA24070 (PubMed:6254986).Curated
Sequence conflicti496 – 51015Missing in CAA24070 (PubMed:6254986).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00694 Genomic DNA. Translation: CAA24070.1.
AJ011856 Genomic DNA. Translation: CAA09824.1.
X14910 Genomic DNA. Translation: CAA33036.1.
PIRiA00468. ODBY1.
S78640.
RefSeqiNP_009305.1. NC_001224.1.

Genome annotation databases

EnsemblFungiiQ0045; Q0045; Q0045.
GeneIDi854598.
KEGGisce:Q0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00694 Genomic DNA. Translation: CAA24070.1.
AJ011856 Genomic DNA. Translation: CAA09824.1.
X14910 Genomic DNA. Translation: CAA33036.1.
PIRiA00468. ODBY1.
S78640.
RefSeqiNP_009305.1. NC_001224.1.

3D structure databases

ProteinModelPortaliP00401.
SMRiP00401. Positions 2-530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34790. 44 interactions.
IntActiP00401. 6 interactions.
MINTiMINT-4981480.

Protein family/group databases

TCDBi3.D.4.8.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

MaxQBiP00401.
PeptideAtlasiP00401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiQ0045; Q0045; Q0045.
GeneIDi854598.
KEGGisce:Q0045.

Organism-specific databases

CYGDiQ0045.
EuPathDBiFungiDB:Q0045.
SGDiS000007260. COX1.

Phylogenomic databases

eggNOGiCOG0843.
GeneTreeiENSGT00390000001518.
HOGENOMiHOG000085274.
InParanoidiP00401.
KOiK02256.
OMAiAALCTNV.
OrthoDBiEOG72C58S.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciMetaCyc:Q0045-MONOMER.
YEAST:Q0045-MONOMER.

Miscellaneous databases

NextBioi977080.
PROiP00401.

Gene expression databases

GenevestigatoriP00401.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrome oxidase."
    Bonitz S.G., Coruzzi G., Thalenfeld B.E., Tzagoloff A., Macino G.
    J. Biol. Chem. 255:11927-11941(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 24657 / D273-10B.
  2. "The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae."
    Foury F., Roganti T., Lecrenier N., Purnelle B.
    FEBS Lett. 440:325-331(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "Sequence of the yeast mitochondrial OX13/OL12 promoter region."
    Seraphin B., Simon M., Jacq C., Faye G.
    Nucleic Acids Res. 17:4886-4886(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
    Strain: ATCC 24657 / D273-10B.
  4. "Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method."
    Geier B.M., Schagger H., Ortwein C., Link T.A., Hagen W.R., Brandt U., Von Jagow G.
    Eur. J. Biochem. 227:296-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE CYTOCHROME C OXIDASE COMPLEX.

Entry informationi

Entry nameiCOX1_YEAST
AccessioniPrimary (citable) accession number: P00401
Secondary accession number(s): Q9ZZX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 23, 2003
Last modified: April 29, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.