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P00399

- COX1_DROME

UniProt

P00399 - COX1_DROME

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Protein

Cytochrome c oxidase subunit 1

Gene

mt:CoI

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Iron (heme A axial ligand)Curated
Metal bindingi238 – 2381Copper BCurated
Metal bindingi242 – 2421Copper BCurated
Metal bindingi288 – 2881Copper BCurated
Metal bindingi289 – 2891Copper BCurated
Metal bindingi374 – 3741Iron (heme A3 axial ligand)Curated
Metal bindingi376 – 3761Iron (heme A axial ligand)Curated

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
  2. mitochondrial DNA replication Source: FlyBase
  3. oxidative phosphorylation Source: UniProtKB-UniPathway
  4. sleep Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_207898. Respiratory electron transport.
REACT_269710. Orphan transporters.
UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:mt:CoI
Synonyms:CoI
Encoded oniMitochondrion
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Mitochondrion

Organism-specific databases

FlyBaseiFBgn0013674. mt:CoI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521HelicalSequence AnalysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence AnalysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence AnalysisAdd
BLAST
Transmembranei232 – 25221HelicalSequence AnalysisAdd
BLAST
Transmembranei266 – 28621HelicalSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Transmembranei336 – 35621HelicalSequence AnalysisAdd
BLAST
Transmembranei378 – 39821HelicalSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST
Transmembranei450 – 47021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-KW
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Cytochrome c oxidase subunit 1PRO_0000183325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki238 ↔ 2421'-histidyl-3'-tyrosine (His-Tyr)By similarity

Expressioni

Gene expression databases

BgeeiP00399.

Structurei

3D structure databases

ProteinModelPortaliP00399.
SMRiP00399. Positions 3-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
GeneTreeiENSGT00390000001518.
InParanoidiP00399.
OMAiPGIQIFS.
OrthoDBiEOG7Q8CMW.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00399-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
SRQWLFSTNH KDIGTLYFIF GAWAGMVGTS LSILIRAELG HPGALIGDDQ
60 70 80 90 100
IYNVIVTAHA FIMIFFMVMP IMIGGFGNWL VPLMLGAPDM AFPRMNNMSF
110 120 130 140 150
WLLPPALSLL LVSSMVENGA GTGWTVYPPL SAGIAHGGAS VDLAIFSLHL
160 170 180 190 200
AGISSILGAV NFITTVINMR STGISLDRMP LFVWSVVITA LLLLLSLPVL
210 220 230 240 250
AGAITMLLTD RNLNTSFFDP AGGGDPILYQ HLFWFFGHPE VYILILPGFG
260 270 280 290 300
MISHIISQES GKKETFGSLG MIYAMLAIGL LGFIVWAHHM FTVGMDVDTR
310 320 330 340 350
AYFTSATMII AVPTGIKIFS WLATLHGTQL SYSPAILWAL GFVFLFTVGG
360 370 380 390 400
LTGVVLANSS VDIILHDTYY VVAHFHYVLS MGAVFAIMAG FIHWYPLFTG
410 420 430 440 450
LTLNNKWLKS HFIIMFIGVN LTFFPQHFLG LAGMPRRYSD YPDAYTTWNI
460 470 480 490 500
VSTIGSTISL LGILFFFFII WESLVSQRQV IYPIQLNSSI EWYQNTPPAE
510
HSYSELPLLT N
Length:511
Mass (Da):56,372
Last modified:January 9, 2013 - v2
Checksum:i332DAFF298EBBA13
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti127 – 1271Y → F in strain: Zimbabwe. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01404 Genomic DNA. Translation: AAB59239.1.
U37541 Genomic DNA. Translation: AAC47812.2.
AF200828 Genomic DNA. Translation: AAF77227.1.
AF200829 Genomic DNA. Translation: AAF77245.1.
AJ400907 Genomic DNA. Translation: CAB91052.2.
FJ190105 Genomic DNA. Translation: ACI28543.1.
FJ190106 Genomic DNA. Translation: ACI28556.1.
FJ190107 Genomic DNA. Translation: ACI28569.1.
FJ190108 Genomic DNA. Translation: ACI28582.1.
FJ190109 Genomic DNA. Translation: ACI28595.1.
FJ190110 Genomic DNA. Translation: ACI28608.1.
GQ229519 mRNA. Translation: ACT21544.1.
JX266575 Genomic DNA. Translation: AFR59645.1.
JX266576 Genomic DNA. Translation: AFR59658.1.
JX266577 Genomic DNA. Translation: AFR59671.1.
JX266578 Genomic DNA. Translation: AFR59684.1.
JX266579 Genomic DNA. Translation: AFR59697.1.
JX266580 Genomic DNA. Translation: AFR59710.1.
JQ686694 Genomic DNA. Translation: AFP47060.1.
JQ686695 Genomic DNA. Translation: AFP47073.1.
JQ686696 Genomic DNA. Translation: AFP47086.1.
JQ686698 Genomic DNA. Translation: AFP47112.1.
JQ686699 Genomic DNA. Translation: AFP47125.1.
M57910 Genomic DNA. Translation: AAB02282.1.
M18022 Genomic DNA. Translation: AAA65480.2.
AY383542 Genomic DNA. Translation: AAQ92343.1.
U51619 Genomic DNA. Translation: AAB68481.2.
PIRiA93307. ODFF1.
RefSeqiYP_009047267.1. NC_024511.2.

Genome annotation databases

EnsemblMetazoaiFBtr0100861; FBpp0100176; FBgn0013674.
GeneIDi19893533.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01404 Genomic DNA. Translation: AAB59239.1 .
U37541 Genomic DNA. Translation: AAC47812.2 .
AF200828 Genomic DNA. Translation: AAF77227.1 .
AF200829 Genomic DNA. Translation: AAF77245.1 .
AJ400907 Genomic DNA. Translation: CAB91052.2 .
FJ190105 Genomic DNA. Translation: ACI28543.1 .
FJ190106 Genomic DNA. Translation: ACI28556.1 .
FJ190107 Genomic DNA. Translation: ACI28569.1 .
FJ190108 Genomic DNA. Translation: ACI28582.1 .
FJ190109 Genomic DNA. Translation: ACI28595.1 .
FJ190110 Genomic DNA. Translation: ACI28608.1 .
GQ229519 mRNA. Translation: ACT21544.1 .
JX266575 Genomic DNA. Translation: AFR59645.1 .
JX266576 Genomic DNA. Translation: AFR59658.1 .
JX266577 Genomic DNA. Translation: AFR59671.1 .
JX266578 Genomic DNA. Translation: AFR59684.1 .
JX266579 Genomic DNA. Translation: AFR59697.1 .
JX266580 Genomic DNA. Translation: AFR59710.1 .
JQ686694 Genomic DNA. Translation: AFP47060.1 .
JQ686695 Genomic DNA. Translation: AFP47073.1 .
JQ686696 Genomic DNA. Translation: AFP47086.1 .
JQ686698 Genomic DNA. Translation: AFP47112.1 .
JQ686699 Genomic DNA. Translation: AFP47125.1 .
M57910 Genomic DNA. Translation: AAB02282.1 .
M18022 Genomic DNA. Translation: AAA65480.2 .
AY383542 Genomic DNA. Translation: AAQ92343.1 .
U51619 Genomic DNA. Translation: AAB68481.2 .
PIRi A93307. ODFF1.
RefSeqi YP_009047267.1. NC_024511.2.

3D structure databases

ProteinModelPortali P00399.
SMRi P00399. Positions 3-505.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0100861 ; FBpp0100176 ; FBgn0013674 .
GeneIDi 19893533.

Organism-specific databases

FlyBasei FBgn0013674. mt:CoI.

Phylogenomic databases

eggNOGi COG0843.
GeneTreei ENSGT00390000001518.
InParanoidi P00399.
OMAi PGIQIFS.
OrthoDBi EOG7Q8CMW.

Enzyme and pathway databases

UniPathwayi UPA00705 .
Reactomei REACT_207898. Respiratory electron transport.
REACT_269710. Orphan transporters.

Miscellaneous databases

GenomeRNAii 192469.
NextBioi 842180.
PROi P00399.

Gene expression databases

Bgeei P00399.

Family and domain databases

Gene3Di 1.20.210.10. 1 hit.
InterProi IPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view ]
PANTHERi PTHR10422. PTHR10422. 1 hit.
Pfami PF00115. COX1. 1 hit.
[Graphical view ]
PRINTSi PR01165. CYCOXIDASEI.
SUPFAMi SSF81442. SSF81442. 1 hit.
PROSITEi PS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code."
    de Bruijn M.H.L.
    Nature 304:234-241(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide sequence and evolutionary comparisons."
    Lewis D.L., Farr C.L., Kaguni L.S.
    Insect Mol. Biol. 4:263-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Comparative genomics of mitochondrial DNA in members of the Drosophila melanogaster subgroup."
    Ballard J.W.O.
    J. Mol. Evol. 51:48-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PHE-127.
    Strain: Oregon-R and Zimbabwe.
  4. "I-R system of hybrid dysgenesis in Drosophila melanogaster: analysis of the mitochondrial DNA in reactive strains exhibiting different potentials for I factor transposition."
    Azou Y., Bregliano J.C.
    Heredity 86:110-116(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Paris.
  5. "Variation in mitochondrial genotype has substantial lifespan effects which may be modulated by nuclear background."
    Clancy D.J.
    Aging Cell 7:795-804(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Alstonville, Brownsville, Dahomey, Japan, Mysore and W1118.
  6. "Characterization of mature mitochondrial transcripts in Drosophila, and the implications for the tRNA punctuation model in arthropods."
    Stewart J.B., Beckenbach A.T.
    Gene 445:49-57(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION OF PROBABLE INITIATION SITE.
    Strain: Oregon-R.
  7. "Mitochondria, maternal inheritance, and male aging."
    Camus M.F., Clancy D.J., Dowling D.K.
    Curr. Biol. 22:1717-1721(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Barcelona, Hawaii, Israel, Madang, Puerto Montt and Sweden.
  8. "A cytoplasmic suppressor of a nuclear mutation affecting mitochondrial functions in Drosophila."
    Chen S., Oliveira M.T., Sanz A., Kemppainen E., Fukuoh A., Schlicht B., Kaguni L.S., Jacobs H.T.
    Genetics 192:483-493(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BER1, Canton-S, CO3, Oregon-RC and QI2.
  9. "Evolution of Drosophila mitochondrial DNA and the history of the melanogaster subgroup."
    Satta Y., Takahata N.
    Proc. Natl. Acad. Sci. U.S.A. 87:9558-9562(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-498.
    Strain: SP1.
  10. "Analysis of nucleotide substitutions of mitochondrial DNAs in Drosophila melanogaster and its sibling species."
    Satta Y., Ishiwa H., Chigusa S.I.
    Mol. Biol. Evol. 4:638-650(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
  11. "Comparative genomics of Drosophila mtDNA: Novel features of conservation and change across functional domains and lineages."
    Montooth K.L., Abt D.N., Hofmann J.W., Rand D.M.
    J. Mol. Evol. 69:94-114(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-10, IDENTIFICATION OF PROBABLE INITIATION SITE.
  12. "Collembola as alternative prey sustaining spiders in arable ecosystems: prey detection within predators using molecular markers."
    Agusti N., Shayler S.P., Harwood J.D., Vaughan I.P., Sunderland K.D., Symondson W.O.
    Mol. Ecol. 12:3467-3475(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-248.
  13. "Mitochondrial DNA phylogenies for the Drosophila obscura group."
    Gleason J.M., Caccone A., Moriyama E.N., White K.P., Powell J.R.
    Evolution 51:433-440(1997)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-390.
    Strain: Australia 13.

Entry informationi

Entry nameiCOX1_DROME
AccessioniPrimary (citable) accession number: P00399
Secondary accession number(s): B6E0P3
, O21461, Q34349, Q6TXT7, Q7GFY7, Q7GHI7, Q9MGN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 9, 2013
Last modified: November 26, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

There is no mitochondrial-type translation initiation codon present in frame in the sequence. In PubMed:19533212, the authors suggest the presence of a novel start codon coding for either Pro or Ser in Drosophila CoI transcripts. In PubMed:19540318, further evidence for the presence of the same start codon coding for Ser in D.melanogaster CoI transcript is presented.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3