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P00399

- COX1_DROME

UniProt

P00399 - COX1_DROME

Protein

Cytochrome c oxidase subunit 1

Gene

mt:CoI

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (09 Jan 2013)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi59 – 591Iron (heme A axial ligand)Curated
    Metal bindingi238 – 2381Copper BCurated
    Metal bindingi242 – 2421Copper BCurated
    Metal bindingi288 – 2881Copper BCurated
    Metal bindingi289 – 2891Copper BCurated
    Metal bindingi374 – 3741Iron (heme A3 axial ligand)Curated
    Metal bindingi376 – 3761Iron (heme A axial ligand)Curated

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro
    2. oxidative phosphorylation Source: UniProtKB-UniPathway
    3. sleep Source: FlyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00705.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:mt:CoI
    Synonyms:CoI
    Encoded oniMitochondrion
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Mitochondrion

    Organism-specific databases

    FlyBaseiFBgn0013674. mt:CoI.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 511511Cytochrome c oxidase subunit 1PRO_0000183325Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki238 ↔ 2421'-histidyl-3'-tyrosine (His-Tyr)By similarity

    Expressioni

    Gene expression databases

    BgeeiP00399.

    Structurei

    3D structure databases

    ProteinModelPortaliP00399.
    SMRiP00399. Positions 3-505.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei143 – 16321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei181 – 20121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei266 – 28621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei336 – 35621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei378 – 39821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei412 – 43221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei450 – 47021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0843.
    GeneTreeiENSGT00390000001518.
    InParanoidiP00399.
    KOiK02256.
    OMAiPGIQIFS.
    OrthoDBiEOG7Q8CMW.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00399-1 [UniParc]FASTAAdd to Basket

    « Hide

    SRQWLFSTNH KDIGTLYFIF GAWAGMVGTS LSILIRAELG HPGALIGDDQ    50
    IYNVIVTAHA FIMIFFMVMP IMIGGFGNWL VPLMLGAPDM AFPRMNNMSF 100
    WLLPPALSLL LVSSMVENGA GTGWTVYPPL SAGIAHGGAS VDLAIFSLHL 150
    AGISSILGAV NFITTVINMR STGISLDRMP LFVWSVVITA LLLLLSLPVL 200
    AGAITMLLTD RNLNTSFFDP AGGGDPILYQ HLFWFFGHPE VYILILPGFG 250
    MISHIISQES GKKETFGSLG MIYAMLAIGL LGFIVWAHHM FTVGMDVDTR 300
    AYFTSATMII AVPTGIKIFS WLATLHGTQL SYSPAILWAL GFVFLFTVGG 350
    LTGVVLANSS VDIILHDTYY VVAHFHYVLS MGAVFAIMAG FIHWYPLFTG 400
    LTLNNKWLKS HFIIMFIGVN LTFFPQHFLG LAGMPRRYSD YPDAYTTWNI 450
    VSTIGSTISL LGILFFFFII WESLVSQRQV IYPIQLNSSI EWYQNTPPAE 500
    HSYSELPLLT N 511
    Length:511
    Mass (Da):56,372
    Last modified:January 9, 2013 - v2
    Checksum:i332DAFF298EBBA13
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti127 – 1271Y → F in strain: Zimbabwe. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01404 Genomic DNA. Translation: AAB59239.1.
    U37541 Genomic DNA. Translation: AAC47812.2.
    AF200828 Genomic DNA. Translation: AAF77227.1.
    AF200829 Genomic DNA. Translation: AAF77245.1.
    AJ400907 Genomic DNA. Translation: CAB91052.2.
    FJ190105 Genomic DNA. Translation: ACI28543.1.
    FJ190106 Genomic DNA. Translation: ACI28556.1.
    FJ190107 Genomic DNA. Translation: ACI28569.1.
    FJ190108 Genomic DNA. Translation: ACI28582.1.
    FJ190109 Genomic DNA. Translation: ACI28595.1.
    FJ190110 Genomic DNA. Translation: ACI28608.1.
    GQ229519 mRNA. Translation: ACT21544.1.
    JX266575 Genomic DNA. Translation: AFR59645.1.
    JX266576 Genomic DNA. Translation: AFR59658.1.
    JX266577 Genomic DNA. Translation: AFR59671.1.
    JX266578 Genomic DNA. Translation: AFR59684.1.
    JX266579 Genomic DNA. Translation: AFR59697.1.
    JX266580 Genomic DNA. Translation: AFR59710.1.
    JQ686694 Genomic DNA. Translation: AFP47060.1.
    JQ686695 Genomic DNA. Translation: AFP47073.1.
    JQ686696 Genomic DNA. Translation: AFP47086.1.
    JQ686698 Genomic DNA. Translation: AFP47112.1.
    JQ686699 Genomic DNA. Translation: AFP47125.1.
    M57910 Genomic DNA. Translation: AAB02282.1.
    M18022 Genomic DNA. Translation: AAA65480.2.
    AY383542 Genomic DNA. Translation: AAQ92343.1.
    U51619 Genomic DNA. Translation: AAB68481.2.
    PIRiA93307. ODFF1.
    RefSeqiNP_008278.1. NC_001709.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0100861; FBpp0100176; FBgn0013674.
    GeneIDi192469.
    KEGGidme:COX1.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01404 Genomic DNA. Translation: AAB59239.1 .
    U37541 Genomic DNA. Translation: AAC47812.2 .
    AF200828 Genomic DNA. Translation: AAF77227.1 .
    AF200829 Genomic DNA. Translation: AAF77245.1 .
    AJ400907 Genomic DNA. Translation: CAB91052.2 .
    FJ190105 Genomic DNA. Translation: ACI28543.1 .
    FJ190106 Genomic DNA. Translation: ACI28556.1 .
    FJ190107 Genomic DNA. Translation: ACI28569.1 .
    FJ190108 Genomic DNA. Translation: ACI28582.1 .
    FJ190109 Genomic DNA. Translation: ACI28595.1 .
    FJ190110 Genomic DNA. Translation: ACI28608.1 .
    GQ229519 mRNA. Translation: ACT21544.1 .
    JX266575 Genomic DNA. Translation: AFR59645.1 .
    JX266576 Genomic DNA. Translation: AFR59658.1 .
    JX266577 Genomic DNA. Translation: AFR59671.1 .
    JX266578 Genomic DNA. Translation: AFR59684.1 .
    JX266579 Genomic DNA. Translation: AFR59697.1 .
    JX266580 Genomic DNA. Translation: AFR59710.1 .
    JQ686694 Genomic DNA. Translation: AFP47060.1 .
    JQ686695 Genomic DNA. Translation: AFP47073.1 .
    JQ686696 Genomic DNA. Translation: AFP47086.1 .
    JQ686698 Genomic DNA. Translation: AFP47112.1 .
    JQ686699 Genomic DNA. Translation: AFP47125.1 .
    M57910 Genomic DNA. Translation: AAB02282.1 .
    M18022 Genomic DNA. Translation: AAA65480.2 .
    AY383542 Genomic DNA. Translation: AAQ92343.1 .
    U51619 Genomic DNA. Translation: AAB68481.2 .
    PIRi A93307. ODFF1.
    RefSeqi NP_008278.1. NC_001709.1.

    3D structure databases

    ProteinModelPortali P00399.
    SMRi P00399. Positions 3-505.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0100861 ; FBpp0100176 ; FBgn0013674 .
    GeneIDi 192469.
    KEGGi dme:COX1.

    Organism-specific databases

    CTDi 4512.
    FlyBasei FBgn0013674. mt:CoI.

    Phylogenomic databases

    eggNOGi COG0843.
    GeneTreei ENSGT00390000001518.
    InParanoidi P00399.
    KOi K02256.
    OMAi PGIQIFS.
    OrthoDBi EOG7Q8CMW.

    Enzyme and pathway databases

    UniPathwayi UPA00705 .

    Miscellaneous databases

    GenomeRNAii 192469.
    NextBioi 842180.
    PROi P00399.

    Gene expression databases

    Bgeei P00399.

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code."
      de Bruijn M.H.L.
      Nature 304:234-241(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide sequence and evolutionary comparisons."
      Lewis D.L., Farr C.L., Kaguni L.S.
      Insect Mol. Biol. 4:263-278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Comparative genomics of mitochondrial DNA in members of the Drosophila melanogaster subgroup."
      Ballard J.W.O.
      J. Mol. Evol. 51:48-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PHE-127.
      Strain: Oregon-R and Zimbabwe.
    4. "I-R system of hybrid dysgenesis in Drosophila melanogaster: analysis of the mitochondrial DNA in reactive strains exhibiting different potentials for I factor transposition."
      Azou Y., Bregliano J.C.
      Heredity 86:110-116(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Paris.
    5. "Variation in mitochondrial genotype has substantial lifespan effects which may be modulated by nuclear background."
      Clancy D.J.
      Aging Cell 7:795-804(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Alstonville, Brownsville, Dahomey, Japan, Mysore and W1118.
    6. "Characterization of mature mitochondrial transcripts in Drosophila, and the implications for the tRNA punctuation model in arthropods."
      Stewart J.B., Beckenbach A.T.
      Gene 445:49-57(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION OF PROBABLE INITIATION SITE.
      Strain: Oregon-R.
    7. "Mitochondria, maternal inheritance, and male aging."
      Camus M.F., Clancy D.J., Dowling D.K.
      Curr. Biol. 22:1717-1721(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Barcelona, Hawaii, Israel, Madang, Puerto Montt and Sweden.
    8. "A cytoplasmic suppressor of a nuclear mutation affecting mitochondrial functions in Drosophila."
      Chen S., Oliveira M.T., Sanz A., Kemppainen E., Fukuoh A., Schlicht B., Kaguni L.S., Jacobs H.T.
      Genetics 192:483-493(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BER1, Canton-S, CO3, Oregon-RC and QI2.
    9. "Evolution of Drosophila mitochondrial DNA and the history of the melanogaster subgroup."
      Satta Y., Takahata N.
      Proc. Natl. Acad. Sci. U.S.A. 87:9558-9562(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-498.
      Strain: SP1.
    10. "Analysis of nucleotide substitutions of mitochondrial DNAs in Drosophila melanogaster and its sibling species."
      Satta Y., Ishiwa H., Chigusa S.I.
      Mol. Biol. Evol. 4:638-650(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-102.
    11. "Comparative genomics of Drosophila mtDNA: Novel features of conservation and change across functional domains and lineages."
      Montooth K.L., Abt D.N., Hofmann J.W., Rand D.M.
      J. Mol. Evol. 69:94-114(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-10, IDENTIFICATION OF PROBABLE INITIATION SITE.
    12. "Collembola as alternative prey sustaining spiders in arable ecosystems: prey detection within predators using molecular markers."
      Agusti N., Shayler S.P., Harwood J.D., Vaughan I.P., Sunderland K.D., Symondson W.O.
      Mol. Ecol. 12:3467-3475(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-248.
    13. "Mitochondrial DNA phylogenies for the Drosophila obscura group."
      Gleason J.M., Caccone A., Moriyama E.N., White K.P., Powell J.R.
      Evolution 51:433-440(1997)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 231-390.
      Strain: Australia 13.

    Entry informationi

    Entry nameiCOX1_DROME
    AccessioniPrimary (citable) accession number: P00399
    Secondary accession number(s): B6E0P3
    , O21461, Q34349, Q6TXT7, Q7GFY7, Q7GHI7, Q9MGN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 9, 2013
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Caution

    There is no mitochondrial-type translation initiation codon present in frame in the sequence. In PubMed:19533212, the authors suggest the presence of a novel start codon coding for either Pro or Ser in Drosophila CoI transcripts. In PubMed:19540318, further evidence for the presence of the same start codon coding for Ser in D.melanogaster CoI transcript is presented.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3