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P00396

- COX1_BOVIN

UniProt

P00396 - COX1_BOVIN

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Protein

Cytochrome c oxidase subunit 1

Gene

MT-CO1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron (heme A axial ligand)1 Publication
Metal bindingi240 – 2401Copper B1 Publication
Binding sitei244 – 2441OxygenCurated
Metal bindingi290 – 2901Copper B1 Publication
Metal bindingi291 – 2911Copper B1 Publication
Metal bindingi368 – 3681Magnesium; shared with chain II1 Publication
Metal bindingi376 – 3761Iron (heme A3 axial ligand)1 Publication
Metal bindingi378 – 3781Iron (heme A axial ligand)1 Publication

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
  2. hydrogen ion transmembrane transport Source: GOC
  3. oxidative phosphorylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP00396.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:MT-CO1
Synonyms:COI, COXI, MTCO1
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Mitochondrial matrix1 PublicationAdd
BLAST
Transmembranei12 – 4029Helical; Name=IAdd
BLAST
Topological domaini41 – 5010Mitochondrial intermembrane1 Publication
Transmembranei51 – 8636Helical; Name=IIAdd
BLAST
Topological domaini87 – 948Mitochondrial matrix1 Publication
Transmembranei95 – 11723Helical; Name=IIIAdd
BLAST
Topological domaini118 – 14023Mitochondrial intermembrane1 PublicationAdd
BLAST
Transmembranei141 – 17030Helical; Name=IVAdd
BLAST
Topological domaini171 – 18212Mitochondrial matrix1 PublicationAdd
BLAST
Transmembranei183 – 21230Helical; Name=VAdd
BLAST
Topological domaini213 – 22715Mitochondrial intermembrane1 PublicationAdd
BLAST
Transmembranei228 – 26134Helical; Name=VIAdd
BLAST
Topological domaini262 – 2698Mitochondrial matrix1 Publication
Transmembranei270 – 28617Helical; Name=VIIAdd
BLAST
Topological domaini287 – 29812Mitochondrial intermembrane1 PublicationAdd
BLAST
Transmembranei299 – 32729Helical; Name=VIIIAdd
BLAST
Topological domaini328 – 3358Mitochondrial matrix
Transmembranei336 – 35722Helical; Name=IXAdd
BLAST
Topological domaini358 – 37013Mitochondrial intermembraneAdd
BLAST
Transmembranei371 – 40030Helical; Name=XAdd
BLAST
Topological domaini401 – 4066Mitochondrial matrix1 Publication
Transmembranei407 – 43327Helical; Name=XIAdd
BLAST
Topological domaini434 – 44613Mitochondrial intermembraneAdd
BLAST
Transmembranei447 – 47832Helical; Name=XIIAdd
BLAST
Topological domaini479 – 51436Mitochondrial matrix1 PublicationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-KW
  3. respiratory chain complex IV Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Cytochrome c oxidase subunit 1PRO_0000183294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine1 Publication
Cross-linki240 ↔ 2441'-histidyl-3'-tyrosine (His-Tyr)1 Publication

Post-translational modificationi

His-240 and Tyr-244 are involved in the formation of a copper-coordinated covalent cross-link at the active site of the catalytic subunit I.

Keywords - PTMi

Formylation

Interactioni

Protein-protein interaction databases

DIPiDIP-60937N.

Structurei

Secondary structure

1
514
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 65Combined sources
Helixi12 – 4130Combined sources
Beta strandi42 – 454Combined sources
Beta strandi47 – 493Combined sources
Helixi51 – 6717Combined sources
Helixi70 – 745Combined sources
Helixi77 – 8610Combined sources
Helixi95 – 1039Combined sources
Helixi105 – 11713Combined sources
Turni125 – 1284Combined sources
Turni130 – 1334Combined sources
Turni135 – 1384Combined sources
Helixi142 – 17029Combined sources
Helixi178 – 1803Combined sources
Helixi183 – 21432Combined sources
Helixi222 – 2243Combined sources
Helixi228 – 26134Combined sources
Helixi270 – 28314Combined sources
Helixi288 – 2914Combined sources
Helixi293 – 2953Combined sources
Helixi299 – 31113Combined sources
Helixi313 – 32715Combined sources
Helixi336 – 35924Combined sources
Helixi361 – 3677Combined sources
Helixi371 – 38111Combined sources
Helixi384 – 40118Combined sources
Helixi407 – 42519Combined sources
Helixi427 – 4337Combined sources
Beta strandi437 – 4393Combined sources
Helixi445 – 4473Combined sources
Helixi448 – 47831Combined sources
Beta strandi481 – 4844Combined sources
Helixi488 – 4903Combined sources
Helixi492 – 4943Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OCCX-ray2.80A/N1-514[»]
1OCOX-ray2.80A/N1-514[»]
1OCRX-ray2.35A/N1-514[»]
1OCZX-ray2.90A/N1-514[»]
1V54X-ray1.80A/N1-514[»]
1V55X-ray1.90A/N1-514[»]
2DYRX-ray1.80A/N1-514[»]
2DYSX-ray2.20A/N1-514[»]
2EIJX-ray1.90A/N1-514[»]
2EIKX-ray2.10A/N1-514[»]
2EILX-ray2.10A/N1-514[»]
2EIMX-ray2.60A/N1-514[»]
2EINX-ray2.70A/N1-514[»]
2OCCX-ray2.30A/N1-514[»]
2Y69X-ray1.95A/N1-514[»]
2YBBelectron microscopy19.00L1-514[»]
2ZXWX-ray2.50A/N1-514[»]
3ABKX-ray2.00A/N1-514[»]
3ABLX-ray2.10A/N1-514[»]
3ABMX-ray1.95A/N1-514[»]
3AG1X-ray2.20A/N1-514[»]
3AG2X-ray1.80A/N1-514[»]
3AG3X-ray1.80A/N1-514[»]
3AG4X-ray2.05A/N1-514[»]
3ASNX-ray3.00A/N1-514[»]
3ASOX-ray2.30A/N1-514[»]
3WG7X-ray1.90A/N1-514[»]
ProteinModelPortaliP00396.
SMRiP00396. Positions 1-514.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00396.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
HOGENOMiHOG000085274.
HOVERGENiHBG003841.
InParanoidiP00396.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00396-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSLLIRAE LGQPGTLLGD
60 70 80 90 100
DQIYNVVVTA HAFVMIFFMV MPIMIGGFGN WLVPLMIGAP DMAFPRMNNM
110 120 130 140 150
SFWLLPPSFL LLLASSMVEA GAGTGWTVYP PLAGNLAHAG ASVDLTIFSL
160 170 180 190 200
HLAGVSSILG AINFITTIIN MKPPAMSQYQ TPLFVWSVMI TAVLLLLSLP
210 220 230 240 250
VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH PEVYILILPG
260 270 280 290 300
FGMISHIVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGMDVD
310 320 330 340 350
TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMMW ALGFIFLFTV
360 370 380 390 400
GGLTGIVLAN SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM GGFVHWFPLF
410 420 430 440 450
SGYTLNDTWA KIHFAIMFVG VNMTFFPQHF LGLSGMPRRY SDYPDAYTMW
460 470 480 490 500
NTISSMGSFI SLTAVMLMVF IIWEAFASKR EVLTVDLTTT NLEWLNGCPP
510
PYHTFEEPTY VNLK
Length:514
Mass (Da):57,032
Last modified:July 21, 1986 - v1
Checksum:i0D7C807D7FA3996C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA23999.1.
AF490528 Genomic DNA. Translation: AAM08330.1.
AF490529 Genomic DNA. Translation: AAM08343.1.
AF493541 Genomic DNA. Translation: AAM12791.1.
AF493542 Genomic DNA. Translation: AAM12804.1.
PIRiA00464. ODBO1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA. Translation: CAA23999.1 .
AF490528 Genomic DNA. Translation: AAM08330.1 .
AF490529 Genomic DNA. Translation: AAM08343.1 .
AF493541 Genomic DNA. Translation: AAM12791.1 .
AF493542 Genomic DNA. Translation: AAM12804.1 .
PIRi A00464. ODBO1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OCC X-ray 2.80 A/N 1-514 [» ]
1OCO X-ray 2.80 A/N 1-514 [» ]
1OCR X-ray 2.35 A/N 1-514 [» ]
1OCZ X-ray 2.90 A/N 1-514 [» ]
1V54 X-ray 1.80 A/N 1-514 [» ]
1V55 X-ray 1.90 A/N 1-514 [» ]
2DYR X-ray 1.80 A/N 1-514 [» ]
2DYS X-ray 2.20 A/N 1-514 [» ]
2EIJ X-ray 1.90 A/N 1-514 [» ]
2EIK X-ray 2.10 A/N 1-514 [» ]
2EIL X-ray 2.10 A/N 1-514 [» ]
2EIM X-ray 2.60 A/N 1-514 [» ]
2EIN X-ray 2.70 A/N 1-514 [» ]
2OCC X-ray 2.30 A/N 1-514 [» ]
2Y69 X-ray 1.95 A/N 1-514 [» ]
2YBB electron microscopy 19.00 L 1-514 [» ]
2ZXW X-ray 2.50 A/N 1-514 [» ]
3ABK X-ray 2.00 A/N 1-514 [» ]
3ABL X-ray 2.10 A/N 1-514 [» ]
3ABM X-ray 1.95 A/N 1-514 [» ]
3AG1 X-ray 2.20 A/N 1-514 [» ]
3AG2 X-ray 1.80 A/N 1-514 [» ]
3AG3 X-ray 1.80 A/N 1-514 [» ]
3AG4 X-ray 2.05 A/N 1-514 [» ]
3ASN X-ray 3.00 A/N 1-514 [» ]
3ASO X-ray 2.30 A/N 1-514 [» ]
3WG7 X-ray 1.90 A/N 1-514 [» ]
ProteinModelPortali P00396.
SMRi P00396. Positions 1-514.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60937N.

Protein family/group databases

TCDBi 3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0843.
HOGENOMi HOG000085274.
HOVERGENi HBG003841.
InParanoidi P00396.

Enzyme and pathway databases

UniPathwayi UPA00705 .
SABIO-RK P00396.

Miscellaneous databases

EvolutionaryTracei P00396.

Family and domain databases

Gene3Di 1.20.210.10. 1 hit.
InterProi IPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view ]
PANTHERi PTHR10422. PTHR10422. 1 hit.
Pfami PF00115. COX1. 1 hit.
[Graphical view ]
PRINTSi PR01165. CYCOXIDASEI.
SUPFAMi SSF81442. SSF81442. 1 hit.
PROSITEi PS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
    Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
    J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Heart.
  2. "Studies on cytochrome-c oxidase, XIV. The amino-acid sequence of subunit I -- proteinchemical methods for the analysis of a large hydrophobic membrane protein."
    Hensel S., Buse G.
    Biol. Chem. Hoppe-Seyler 371:411-422(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FORMYLATION AT MET-1.
    Tissue: Heart.
  3. "Bos taurus mitochondrial protein coding regions."
    Wettstein P.J.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 65, 66, D and F.
  4. "Evidence for a copper-coordinated histidine-tyrosine cross-link in the active site of cytochrome oxidase."
    Buse G., Soulimane T., Dewor M., Meyer H.E., Blueggel M.
    Protein Sci. 8:985-990(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT BOND.
  5. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
    Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
    Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Tissue: Heart.
  7. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
    Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
    Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
    Tissue: Heart.

Entry informationi

Entry nameiCOX1_BOVIN
AccessioniPrimary (citable) accession number: P00396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3