SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P00396

- COX1_BOVIN

UniProt

P00396 - COX1_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Cytochrome c oxidase subunit 1
Gene
MT-CO1, COI, COXI, MTCO1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron (heme A axial ligand)1 Publication
Metal bindingi240 – 2401Copper B1 Publication
Binding sitei244 – 2441Oxygen Inferred
Metal bindingi290 – 2901Copper B1 Publication
Metal bindingi291 – 2911Copper B1 Publication
Metal bindingi368 – 3681Magnesium; shared with chain II1 Publication
Metal bindingi376 – 3761Iron (heme A3 axial ligand)1 Publication
Metal bindingi378 – 3781Iron (heme A axial ligand)1 Publication

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
  2. hydrogen ion transmembrane transport Source: GOC
  3. oxidative phosphorylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP00396.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:MT-CO1
Synonyms:COI, COXI, MTCO1
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Mitochondrion

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Mitochondrial matrix1 Publication
Add
BLAST
Transmembranei12 – 4029Helical; Name=I
Add
BLAST
Topological domaini41 – 5010Mitochondrial intermembrane1 Publication
Transmembranei51 – 8636Helical; Name=II
Add
BLAST
Topological domaini87 – 948Mitochondrial matrix1 Publication
Transmembranei95 – 11723Helical; Name=III
Add
BLAST
Topological domaini118 – 14023Mitochondrial intermembrane1 Publication
Add
BLAST
Transmembranei141 – 17030Helical; Name=IV
Add
BLAST
Topological domaini171 – 18212Mitochondrial matrix1 Publication
Add
BLAST
Transmembranei183 – 21230Helical; Name=V
Add
BLAST
Topological domaini213 – 22715Mitochondrial intermembrane1 Publication
Add
BLAST
Transmembranei228 – 26134Helical; Name=VI
Add
BLAST
Topological domaini262 – 2698Mitochondrial matrix1 Publication
Transmembranei270 – 28617Helical; Name=VII
Add
BLAST
Topological domaini287 – 29812Mitochondrial intermembrane1 Publication
Add
BLAST
Transmembranei299 – 32729Helical; Name=VIII
Add
BLAST
Topological domaini328 – 3358Mitochondrial matrix
Transmembranei336 – 35722Helical; Name=IX
Add
BLAST
Topological domaini358 – 37013Mitochondrial intermembrane
Add
BLAST
Transmembranei371 – 40030Helical; Name=X
Add
BLAST
Topological domaini401 – 4066Mitochondrial matrix1 Publication
Transmembranei407 – 43327Helical; Name=XI
Add
BLAST
Topological domaini434 – 44613Mitochondrial intermembrane
Add
BLAST
Transmembranei447 – 47832Helical; Name=XII
Add
BLAST
Topological domaini479 – 51436Mitochondrial matrix1 Publication
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. respiratory chain complex IV Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Cytochrome c oxidase subunit 1
PRO_0000183294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-formylmethionine1 Publication
Cross-linki240 ↔ 2441'-histidyl-3'-tyrosine (His-Tyr)1 Publication

Post-translational modificationi

His-240 and Tyr-244 are involved in the formation of a copper-coordinated covalent cross-link at the active site of the catalytic subunit I.

Keywords - PTMi

Formylation

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 65
Helixi12 – 4130
Beta strandi42 – 454
Beta strandi47 – 493
Helixi51 – 6717
Helixi70 – 745
Helixi77 – 8610
Helixi95 – 1039
Helixi105 – 11713
Turni125 – 1284
Turni130 – 1334
Turni135 – 1384
Helixi142 – 17029
Helixi178 – 1803
Helixi183 – 21432
Helixi222 – 2243
Helixi228 – 26134
Helixi270 – 28314
Helixi288 – 2914
Helixi293 – 2953
Helixi299 – 31113
Helixi313 – 32715
Helixi336 – 35924
Helixi361 – 3677
Helixi371 – 38111
Helixi384 – 40118
Helixi407 – 42519
Helixi427 – 4337
Beta strandi437 – 4393
Helixi445 – 4473
Helixi448 – 47831
Beta strandi481 – 4844
Helixi488 – 4903
Helixi492 – 4943

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OCCX-ray2.80A/N1-514[»]
1OCOX-ray2.80A/N1-514[»]
1OCRX-ray2.35A/N1-514[»]
1OCZX-ray2.90A/N1-514[»]
1V54X-ray1.80A/N1-514[»]
1V55X-ray1.90A/N1-514[»]
2DYRX-ray1.80A/N1-514[»]
2DYSX-ray2.20A/N1-514[»]
2EIJX-ray1.90A/N1-514[»]
2EIKX-ray2.10A/N1-514[»]
2EILX-ray2.10A/N1-514[»]
2EIMX-ray2.60A/N1-514[»]
2EINX-ray2.70A/N1-514[»]
2OCCX-ray2.30A/N1-514[»]
2Y69X-ray1.95A/N1-514[»]
2YBBelectron microscopy19.00L1-514[»]
2ZXWX-ray2.50A/N1-514[»]
3ABKX-ray2.00A/N1-514[»]
3ABLX-ray2.10A/N1-514[»]
3ABMX-ray1.95A/N1-514[»]
3AG1X-ray2.20A/N1-514[»]
3AG2X-ray1.80A/N1-514[»]
3AG3X-ray1.80A/N1-514[»]
3AG4X-ray2.05A/N1-514[»]
3ASNX-ray3.00A/N1-514[»]
3ASOX-ray2.30A/N1-514[»]
3WG7X-ray1.90A/N1-514[»]
ProteinModelPortaliP00396.
SMRiP00396. Positions 1-514.

Miscellaneous databases

EvolutionaryTraceiP00396.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
HOGENOMiHOG000085274.
HOVERGENiHBG003841.
InParanoidiP00396.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P00396-1 [UniParc]FASTAAdd to Basket

« Hide

MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSLLIRAE LGQPGTLLGD    50
DQIYNVVVTA HAFVMIFFMV MPIMIGGFGN WLVPLMIGAP DMAFPRMNNM 100
SFWLLPPSFL LLLASSMVEA GAGTGWTVYP PLAGNLAHAG ASVDLTIFSL 150
HLAGVSSILG AINFITTIIN MKPPAMSQYQ TPLFVWSVMI TAVLLLLSLP 200
VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH PEVYILILPG 250
FGMISHIVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGMDVD 300
TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMMW ALGFIFLFTV 350
GGLTGIVLAN SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM GGFVHWFPLF 400
SGYTLNDTWA KIHFAIMFVG VNMTFFPQHF LGLSGMPRRY SDYPDAYTMW 450
NTISSMGSFI SLTAVMLMVF IIWEAFASKR EVLTVDLTTT NLEWLNGCPP 500
PYHTFEEPTY VNLK 514
Length:514
Mass (Da):57,032
Last modified:July 21, 1986 - v1
Checksum:i0D7C807D7FA3996C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00654 Genomic DNA. Translation: CAA23999.1.
AF490528 Genomic DNA. Translation: AAM08330.1.
AF490529 Genomic DNA. Translation: AAM08343.1.
AF493541 Genomic DNA. Translation: AAM12791.1.
AF493542 Genomic DNA. Translation: AAM12804.1.
PIRiA00464. ODBO1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00654 Genomic DNA. Translation: CAA23999.1 .
AF490528 Genomic DNA. Translation: AAM08330.1 .
AF490529 Genomic DNA. Translation: AAM08343.1 .
AF493541 Genomic DNA. Translation: AAM12791.1 .
AF493542 Genomic DNA. Translation: AAM12804.1 .
PIRi A00464. ODBO1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OCC X-ray 2.80 A/N 1-514 [» ]
1OCO X-ray 2.80 A/N 1-514 [» ]
1OCR X-ray 2.35 A/N 1-514 [» ]
1OCZ X-ray 2.90 A/N 1-514 [» ]
1V54 X-ray 1.80 A/N 1-514 [» ]
1V55 X-ray 1.90 A/N 1-514 [» ]
2DYR X-ray 1.80 A/N 1-514 [» ]
2DYS X-ray 2.20 A/N 1-514 [» ]
2EIJ X-ray 1.90 A/N 1-514 [» ]
2EIK X-ray 2.10 A/N 1-514 [» ]
2EIL X-ray 2.10 A/N 1-514 [» ]
2EIM X-ray 2.60 A/N 1-514 [» ]
2EIN X-ray 2.70 A/N 1-514 [» ]
2OCC X-ray 2.30 A/N 1-514 [» ]
2Y69 X-ray 1.95 A/N 1-514 [» ]
2YBB electron microscopy 19.00 L 1-514 [» ]
2ZXW X-ray 2.50 A/N 1-514 [» ]
3ABK X-ray 2.00 A/N 1-514 [» ]
3ABL X-ray 2.10 A/N 1-514 [» ]
3ABM X-ray 1.95 A/N 1-514 [» ]
3AG1 X-ray 2.20 A/N 1-514 [» ]
3AG2 X-ray 1.80 A/N 1-514 [» ]
3AG3 X-ray 1.80 A/N 1-514 [» ]
3AG4 X-ray 2.05 A/N 1-514 [» ]
3ASN X-ray 3.00 A/N 1-514 [» ]
3ASO X-ray 2.30 A/N 1-514 [» ]
3WG7 X-ray 1.90 A/N 1-514 [» ]
ProteinModelPortali P00396.
SMRi P00396. Positions 1-514.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

TCDBi 3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0843.
HOGENOMi HOG000085274.
HOVERGENi HBG003841.
InParanoidi P00396.

Enzyme and pathway databases

UniPathwayi UPA00705 .
SABIO-RK P00396.

Miscellaneous databases

EvolutionaryTracei P00396.

Family and domain databases

Gene3Di 1.20.210.10. 1 hit.
InterProi IPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
[Graphical view ]
PANTHERi PTHR10422. PTHR10422. 1 hit.
Pfami PF00115. COX1. 1 hit.
[Graphical view ]
PRINTSi PR01165. CYCOXIDASEI.
SUPFAMi SSF81442. SSF81442. 1 hit.
PROSITEi PS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
    Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
    J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Heart.
  2. "Studies on cytochrome-c oxidase, XIV. The amino-acid sequence of subunit I -- proteinchemical methods for the analysis of a large hydrophobic membrane protein."
    Hensel S., Buse G.
    Biol. Chem. Hoppe-Seyler 371:411-422(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FORMYLATION AT MET-1.
    Tissue: Heart.
  3. "Bos taurus mitochondrial protein coding regions."
    Wettstein P.J.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 65, 66, D and F.
  4. "Evidence for a copper-coordinated histidine-tyrosine cross-link in the active site of cytochrome oxidase."
    Buse G., Soulimane T., Dewor M., Meyer H.E., Blueggel M.
    Protein Sci. 8:985-990(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COVALENT BOND.
  5. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
    Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
    Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Tissue: Heart.
  7. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
    Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
    Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
    Tissue: Heart.

Entry informationi

Entry nameiCOX1_BOVIN
AccessioniPrimary (citable) accession number: P00396
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi