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P00396

- COX1_BOVIN

UniProt

P00396 - COX1_BOVIN

Protein

Cytochrome c oxidase subunit 1

Gene

MT-CO1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

    Catalytic activityi

    4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611Iron (heme A axial ligand)1 Publication
    Metal bindingi240 – 2401Copper B1 Publication
    Binding sitei244 – 2441OxygenCurated
    Metal bindingi290 – 2901Copper B1 Publication
    Metal bindingi291 – 2911Copper B1 Publication
    Metal bindingi368 – 3681Magnesium; shared with chain II1 Publication
    Metal bindingi376 – 3761Iron (heme A3 axial ligand)1 Publication
    Metal bindingi378 – 3781Iron (heme A axial ligand)1 Publication

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro
    2. hydrogen ion transmembrane transport Source: GOC
    3. oxidative phosphorylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP00396.
    UniPathwayiUPA00705.

    Protein family/group databases

    TCDBi3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
    Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Gene namesi
    Name:MT-CO1
    Synonyms:COI, COXI, MTCO1
    Encoded oniMitochondrion
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Mitochondrion

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. respiratory chain complex IV Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 514514Cytochrome c oxidase subunit 1PRO_0000183294Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-formylmethionine1 Publication
    Cross-linki240 ↔ 2441'-histidyl-3'-tyrosine (His-Tyr)1 Publication

    Post-translational modificationi

    His-240 and Tyr-244 are involved in the formation of a copper-coordinated covalent cross-link at the active site of the catalytic subunit I.

    Keywords - PTMi

    Formylation

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60937N.

    Structurei

    Secondary structure

    1
    514
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 65
    Helixi12 – 4130
    Beta strandi42 – 454
    Beta strandi47 – 493
    Helixi51 – 6717
    Helixi70 – 745
    Helixi77 – 8610
    Helixi95 – 1039
    Helixi105 – 11713
    Turni125 – 1284
    Turni130 – 1334
    Turni135 – 1384
    Helixi142 – 17029
    Helixi178 – 1803
    Helixi183 – 21432
    Helixi222 – 2243
    Helixi228 – 26134
    Helixi270 – 28314
    Helixi288 – 2914
    Helixi293 – 2953
    Helixi299 – 31113
    Helixi313 – 32715
    Helixi336 – 35924
    Helixi361 – 3677
    Helixi371 – 38111
    Helixi384 – 40118
    Helixi407 – 42519
    Helixi427 – 4337
    Beta strandi437 – 4393
    Helixi445 – 4473
    Helixi448 – 47831
    Beta strandi481 – 4844
    Helixi488 – 4903
    Helixi492 – 4943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OCCX-ray2.80A/N1-514[»]
    1OCOX-ray2.80A/N1-514[»]
    1OCRX-ray2.35A/N1-514[»]
    1OCZX-ray2.90A/N1-514[»]
    1V54X-ray1.80A/N1-514[»]
    1V55X-ray1.90A/N1-514[»]
    2DYRX-ray1.80A/N1-514[»]
    2DYSX-ray2.20A/N1-514[»]
    2EIJX-ray1.90A/N1-514[»]
    2EIKX-ray2.10A/N1-514[»]
    2EILX-ray2.10A/N1-514[»]
    2EIMX-ray2.60A/N1-514[»]
    2EINX-ray2.70A/N1-514[»]
    2OCCX-ray2.30A/N1-514[»]
    2Y69X-ray1.95A/N1-514[»]
    2YBBelectron microscopy19.00L1-514[»]
    2ZXWX-ray2.50A/N1-514[»]
    3ABKX-ray2.00A/N1-514[»]
    3ABLX-ray2.10A/N1-514[»]
    3ABMX-ray1.95A/N1-514[»]
    3AG1X-ray2.20A/N1-514[»]
    3AG2X-ray1.80A/N1-514[»]
    3AG3X-ray1.80A/N1-514[»]
    3AG4X-ray2.05A/N1-514[»]
    3ASNX-ray3.00A/N1-514[»]
    3ASOX-ray2.30A/N1-514[»]
    3WG7X-ray1.90A/N1-514[»]
    ProteinModelPortaliP00396.
    SMRiP00396. Positions 1-514.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP00396.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111Mitochondrial matrix1 PublicationAdd
    BLAST
    Topological domaini41 – 5010Mitochondrial intermembrane1 Publication
    Topological domaini87 – 948Mitochondrial matrix1 Publication
    Topological domaini118 – 14023Mitochondrial intermembrane1 PublicationAdd
    BLAST
    Topological domaini171 – 18212Mitochondrial matrix1 PublicationAdd
    BLAST
    Topological domaini213 – 22715Mitochondrial intermembrane1 PublicationAdd
    BLAST
    Topological domaini262 – 2698Mitochondrial matrix1 Publication
    Topological domaini287 – 29812Mitochondrial intermembrane1 PublicationAdd
    BLAST
    Topological domaini328 – 3358Mitochondrial matrix
    Topological domaini358 – 37013Mitochondrial intermembraneAdd
    BLAST
    Topological domaini401 – 4066Mitochondrial matrix1 Publication
    Topological domaini434 – 44613Mitochondrial intermembraneAdd
    BLAST
    Topological domaini479 – 51436Mitochondrial matrix1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 4029Helical; Name=IAdd
    BLAST
    Transmembranei51 – 8636Helical; Name=IIAdd
    BLAST
    Transmembranei95 – 11723Helical; Name=IIIAdd
    BLAST
    Transmembranei141 – 17030Helical; Name=IVAdd
    BLAST
    Transmembranei183 – 21230Helical; Name=VAdd
    BLAST
    Transmembranei228 – 26134Helical; Name=VIAdd
    BLAST
    Transmembranei270 – 28617Helical; Name=VIIAdd
    BLAST
    Transmembranei299 – 32729Helical; Name=VIIIAdd
    BLAST
    Transmembranei336 – 35722Helical; Name=IXAdd
    BLAST
    Transmembranei371 – 40030Helical; Name=XAdd
    BLAST
    Transmembranei407 – 43327Helical; Name=XIAdd
    BLAST
    Transmembranei447 – 47832Helical; Name=XIIAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0843.
    HOGENOMiHOG000085274.
    HOVERGENiHBG003841.
    InParanoidiP00396.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P00396-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSLLIRAE LGQPGTLLGD    50
    DQIYNVVVTA HAFVMIFFMV MPIMIGGFGN WLVPLMIGAP DMAFPRMNNM 100
    SFWLLPPSFL LLLASSMVEA GAGTGWTVYP PLAGNLAHAG ASVDLTIFSL 150
    HLAGVSSILG AINFITTIIN MKPPAMSQYQ TPLFVWSVMI TAVLLLLSLP 200
    VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH PEVYILILPG 250
    FGMISHIVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGMDVD 300
    TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMMW ALGFIFLFTV 350
    GGLTGIVLAN SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM GGFVHWFPLF 400
    SGYTLNDTWA KIHFAIMFVG VNMTFFPQHF LGLSGMPRRY SDYPDAYTMW 450
    NTISSMGSFI SLTAVMLMVF IIWEAFASKR EVLTVDLTTT NLEWLNGCPP 500
    PYHTFEEPTY VNLK 514
    Length:514
    Mass (Da):57,032
    Last modified:July 21, 1986 - v1
    Checksum:i0D7C807D7FA3996C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA23999.1.
    AF490528 Genomic DNA. Translation: AAM08330.1.
    AF490529 Genomic DNA. Translation: AAM08343.1.
    AF493541 Genomic DNA. Translation: AAM12791.1.
    AF493542 Genomic DNA. Translation: AAM12804.1.
    PIRiA00464. ODBO1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00654 Genomic DNA. Translation: CAA23999.1 .
    AF490528 Genomic DNA. Translation: AAM08330.1 .
    AF490529 Genomic DNA. Translation: AAM08343.1 .
    AF493541 Genomic DNA. Translation: AAM12791.1 .
    AF493542 Genomic DNA. Translation: AAM12804.1 .
    PIRi A00464. ODBO1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OCC X-ray 2.80 A/N 1-514 [» ]
    1OCO X-ray 2.80 A/N 1-514 [» ]
    1OCR X-ray 2.35 A/N 1-514 [» ]
    1OCZ X-ray 2.90 A/N 1-514 [» ]
    1V54 X-ray 1.80 A/N 1-514 [» ]
    1V55 X-ray 1.90 A/N 1-514 [» ]
    2DYR X-ray 1.80 A/N 1-514 [» ]
    2DYS X-ray 2.20 A/N 1-514 [» ]
    2EIJ X-ray 1.90 A/N 1-514 [» ]
    2EIK X-ray 2.10 A/N 1-514 [» ]
    2EIL X-ray 2.10 A/N 1-514 [» ]
    2EIM X-ray 2.60 A/N 1-514 [» ]
    2EIN X-ray 2.70 A/N 1-514 [» ]
    2OCC X-ray 2.30 A/N 1-514 [» ]
    2Y69 X-ray 1.95 A/N 1-514 [» ]
    2YBB electron microscopy 19.00 L 1-514 [» ]
    2ZXW X-ray 2.50 A/N 1-514 [» ]
    3ABK X-ray 2.00 A/N 1-514 [» ]
    3ABL X-ray 2.10 A/N 1-514 [» ]
    3ABM X-ray 1.95 A/N 1-514 [» ]
    3AG1 X-ray 2.20 A/N 1-514 [» ]
    3AG2 X-ray 1.80 A/N 1-514 [» ]
    3AG3 X-ray 1.80 A/N 1-514 [» ]
    3AG4 X-ray 2.05 A/N 1-514 [» ]
    3ASN X-ray 3.00 A/N 1-514 [» ]
    3ASO X-ray 2.30 A/N 1-514 [» ]
    3WG7 X-ray 1.90 A/N 1-514 [» ]
    ProteinModelPortali P00396.
    SMRi P00396. Positions 1-514.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60937N.

    Protein family/group databases

    TCDBi 3.D.4.7.1. the proton-translocating cytochrome oxidase (cox) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0843.
    HOGENOMi HOG000085274.
    HOVERGENi HBG003841.
    InParanoidi P00396.

    Enzyme and pathway databases

    UniPathwayi UPA00705 .
    SABIO-RK P00396.

    Miscellaneous databases

    EvolutionaryTracei P00396.

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome."
      Anderson S., de Bruijn M.H.L., Coulson A.R., Eperon I.C., Sanger F., Young I.G.
      J. Mol. Biol. 156:683-717(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Heart.
    2. "Studies on cytochrome-c oxidase, XIV. The amino-acid sequence of subunit I -- proteinchemical methods for the analysis of a large hydrophobic membrane protein."
      Hensel S., Buse G.
      Biol. Chem. Hoppe-Seyler 371:411-422(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FORMYLATION AT MET-1.
      Tissue: Heart.
    3. "Bos taurus mitochondrial protein coding regions."
      Wettstein P.J.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 65, 66, D and F.
    4. "Evidence for a copper-coordinated histidine-tyrosine cross-link in the active site of cytochrome oxidase."
      Buse G., Soulimane T., Dewor M., Meyer H.E., Blueggel M.
      Protein Sci. 8:985-990(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: COVALENT BOND.
    5. "The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A."
      Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaono R., Yoshikawa S.
      Science 272:1136-1144(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    6. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Tissue: Heart.
    7. "X-ray structure of azide-bound fully oxidized cytochrome c oxidase from bovine heart at 2.9 A resolution."
      Fei M.J., Yamashita E., Inoue N., Yao M., Yamaguchi H., Tsukihara T., Shinzawa-Itoh K., Nakashima R., Yoshikawa S.
      Acta Crystallogr. D 56:529-535(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
      Tissue: Heart.

    Entry informationi

    Entry nameiCOX1_BOVIN
    AccessioniPrimary (citable) accession number: P00396
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3