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Protein

NADH dehydrogenase

Gene

ndh

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation.

Catalytic activityi

NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 4236FADBy similarityAdd
BLAST
Nucleotide bindingi171 – 20838NADBy similarityAdd
BLAST

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: EcoCyc
  • NADH dehydrogenase (ubiquinone) activity Source: EcoCyc

GO - Biological processi

  • aerobic electron transport chain Source: EcoCyc
  • aerobic respiration Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciEcoCyc:NADH-DHII-MONOMER.
ECOL316407:JW1095-MONOMER.
MetaCyc:NADH-DHII-MONOMER.

Protein family/group databases

PeroxiBasei5949. EcoNadDH_K12.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase (EC:1.6.99.3)
Gene namesi
Name:ndh
Ordered Locus Names:b1109, JW1095
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10649. ndh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 434433NADH dehydrogenasePRO_0000079890Add
BLAST

Proteomic databases

PaxDbiP00393.
PRIDEiP00393.

Expressioni

Inductioni

Expressed aerobically and repressed in fermentation.

Interactioni

Protein-protein interaction databases

DIPiDIP-10325N.
IntActiP00393. 9 interactions.
STRINGi511145.b1109.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OZKmodel-A2-386[»]
ProteinModelPortaliP00393.
SMRiP00393. Positions 8-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NADH dehydrogenase family.Curated

Phylogenomic databases

eggNOGiCOG1252.
HOGENOMiHOG000157010.
InParanoidiP00393.
KOiK03885.
OMAiHQMASLV.
OrthoDBiEOG6QZMR6.
PhylomeDBiP00393.

Family and domain databases

InterProiIPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00393-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL
60 70 80 90 100
LHEVATGSLD EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR
110 120 130 140 150
DEKGELLVPE RKIAYDTLVM ALGSTSNDFN TPGVKENCIF LDNPHQARRF
160 170 180 190 200
HQEMLNLFLK YSANLGANGK VNIAIVGGGA TGVELSAELH NAVKQLHSYG
210 220 230 240 250
YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG VRVLTQTMVT
260 270 280 290 300
SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP
310 320 330 340 350
TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN
360 370 380 390 400
GKPLKNYQYK DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL
410 420 430
YRMHQIALHG YFKTGLMMLV GSINRVIRPR LKLH
Length:434
Mass (Da):47,359
Last modified:January 23, 2007 - v2
Checksum:i3C3F4FA0E31E10E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00306 Genomic DNA. Translation: CAA23586.1.
U00096 Genomic DNA. Translation: AAC74193.1.
AP009048 Genomic DNA. Translation: BAA35924.1.
PIRiA00461. DEECR.
RefSeqiNP_415627.1. NC_000913.3.
WP_000211045.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC74193; AAC74193; b1109.
BAA35924; BAA35924; BAA35924.
GeneIDi946792.
KEGGieco:b1109.
PATRICi32117461. VBIEscCol129921_1153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00306 Genomic DNA. Translation: CAA23586.1.
U00096 Genomic DNA. Translation: AAC74193.1.
AP009048 Genomic DNA. Translation: BAA35924.1.
PIRiA00461. DEECR.
RefSeqiNP_415627.1. NC_000913.3.
WP_000211045.1. NZ_CP010445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OZKmodel-A2-386[»]
ProteinModelPortaliP00393.
SMRiP00393. Positions 8-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10325N.
IntActiP00393. 9 interactions.
STRINGi511145.b1109.

Protein family/group databases

PeroxiBasei5949. EcoNadDH_K12.

Proteomic databases

PaxDbiP00393.
PRIDEiP00393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74193; AAC74193; b1109.
BAA35924; BAA35924; BAA35924.
GeneIDi946792.
KEGGieco:b1109.
PATRICi32117461. VBIEscCol129921_1153.

Organism-specific databases

EchoBASEiEB0643.
EcoGeneiEG10649. ndh.

Phylogenomic databases

eggNOGiCOG1252.
HOGENOMiHOG000157010.
InParanoidiP00393.
KOiK03885.
OMAiHQMASLV.
OrthoDBiEOG6QZMR6.
PhylomeDBiP00393.

Enzyme and pathway databases

BioCyciEcoCyc:NADH-DHII-MONOMER.
ECOL316407:JW1095-MONOMER.
MetaCyc:NADH-DHII-MONOMER.

Miscellaneous databases

PROiP00393.

Family and domain databases

InterProiIPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon."
    Young J.G., Rogers B.L., Campbell H.D., Jaworowski A., Shaw D.C.
    Eur. J. Biochem. 116:165-170(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDHNA_ECOLI
AccessioniPrimary (citable) accession number: P00393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.