NADH dehydrogenase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P00393 (DHNA_ECOLI)

Last modified June 16, 2009. Version 91. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
NADH dehydrogenase
EC=1.6.99.3

Gene names

Name:	ndh
Ordered Locus Names:	b1109, JW1095

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation.
Catalytic activity	NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cell membrane.
Induction	Expressed aerobically and repressed in fermentation.
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed Ref.1

Chain

433

NADH dehydrogenase

PRO_0000079890

Regions

Nucleotide binding

36

FAD By similarity

Nucleotide binding

38

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P00393-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3C3F4FA0E31E10E1
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434

47,359

        10         20         30         40         50         60 
MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL LHEVATGSLD 

        70         80         90        100        110        120 
EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR DEKGELLVPE RKIAYDTLVM 

       130        140        150        160        170        180 
ALGSTSNDFN TPGVKENCIF LDNPHQARRF HQEMLNLFLK YSANLGANGK VNIAIVGGGA 

       190        200        210        220        230        240 
TGVELSAELH NAVKQLHSYG YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG 

       250        260        270        280        290        300 
VRVLTQTMVT SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP 

       310        320        330        340        350        360 
TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN GKPLKNYQYK 

       370        380        390        400        410        420 
DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL YRMHQIALHG YFKTGLMMLV 

       430 
GSINRVIRPR LKLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon." Young J.G., Rogers B.L., Campbell H.D., Jaworowski A., Shaw D.C. Eur. J. Biochem. 116:165-170(1981) [PubMed: 6265208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

V00306 Genomic DNA. Translation: CAA23586.1.
U00096 Genomic DNA. Translation: AAC74193.1.
AP009048 Genomic DNA. Translation: BAA35924.1.

PIR

DEECR. A00461.

RefSeq

AP_001735.1.
NP_415627.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OZK	model	-	A	2-386	[»]

ModBase

Protein family/group databases

PeroxiBase

5949. EcoNadDH_K12.

Genome annotation databases

GeneID

GenomeReviews

Gene locus JW1095 in contig AP009048_GR.
Gene locus b1109 in contig U00096_GR.

KEGG

ecj:JW1095.
eco:b1109.

Organism-specific databases

EchoBASE

EcoGene

EG10649. ndh.

CMR

Phylogenomic databases

HOGENOM

OMA

P00393. MVTSADA.

Enzyme and pathway databases

BioCyc

EcoCyc:NADH-DHII-MON.
MetaCyc:NADH-DHII-MON.

Family and domain databases

InterPro

IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR001327. Pyr_OxRdtase_NAD_bd.
[Graphical view]

Pfam

PF00070. Pyr_redox. 2 hits.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]

PRINTS

PR00368. FADPNR.

ProtoNet

Entry information

Entry name

DHNA_ECOLI

Accession

Primary (citable) accession number: P00393

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents