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P00393

- DHNA_ECOLI

UniProt

P00393 - DHNA_ECOLI

Protein

NADH dehydrogenase

Gene

ndh

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation.

    Catalytic activityi

    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 4236FADBy similarityAdd
    BLAST
    Nucleotide bindingi171 – 20838NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: EcoCyc
    2. NADH dehydrogenase (ubiquinone) activity Source: EcoCyc

    GO - Biological processi

    1. aerobic electron transport chain Source: EcoCyc
    2. aerobic respiration Source: EcoCyc

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciEcoCyc:NADH-DHII-MONOMER.
    ECOL316407:JW1095-MONOMER.
    MetaCyc:NADH-DHII-MONOMER.

    Protein family/group databases

    PeroxiBasei5949. EcoNadDH_K12.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase (EC:1.6.99.3)
    Gene namesi
    Name:ndh
    Ordered Locus Names:b1109, JW1095
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10649. ndh.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 434433NADH dehydrogenasePRO_0000079890Add
    BLAST

    Proteomic databases

    PaxDbiP00393.
    PRIDEiP00393.

    Expressioni

    Inductioni

    Expressed aerobically and repressed in fermentation.

    Gene expression databases

    GenevestigatoriP00393.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-10325N.
    IntActiP00393. 9 interactions.
    STRINGi511145.b1109.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OZKmodel-A2-386[»]
    ProteinModelPortaliP00393.
    SMRiP00393. Positions 2-400.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NADH dehydrogenase family.Curated

    Phylogenomic databases

    eggNOGiCOG1252.
    HOGENOMiHOG000157010.
    KOiK03885.
    OMAiALHGVFK.
    OrthoDBiEOG6QZMR6.
    PhylomeDBiP00393.

    Family and domain databases

    InterProiIPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P00393-1 [UniParc]FASTAAdd to Basket

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    MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL    50
    LHEVATGSLD EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR 100
    DEKGELLVPE RKIAYDTLVM ALGSTSNDFN TPGVKENCIF LDNPHQARRF 150
    HQEMLNLFLK YSANLGANGK VNIAIVGGGA TGVELSAELH NAVKQLHSYG 200
    YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG VRVLTQTMVT 250
    SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP 300
    TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN 350
    GKPLKNYQYK DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL 400
    YRMHQIALHG YFKTGLMMLV GSINRVIRPR LKLH 434
    Length:434
    Mass (Da):47,359
    Last modified:January 23, 2007 - v2
    Checksum:i3C3F4FA0E31E10E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00306 Genomic DNA. Translation: CAA23586.1.
    U00096 Genomic DNA. Translation: AAC74193.1.
    AP009048 Genomic DNA. Translation: BAA35924.1.
    PIRiA00461. DEECR.
    RefSeqiNP_415627.1. NC_000913.3.
    YP_489377.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74193; AAC74193; b1109.
    BAA35924; BAA35924; BAA35924.
    GeneIDi12931088.
    946792.
    KEGGiecj:Y75_p1079.
    eco:b1109.
    PATRICi32117461. VBIEscCol129921_1153.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00306 Genomic DNA. Translation: CAA23586.1 .
    U00096 Genomic DNA. Translation: AAC74193.1 .
    AP009048 Genomic DNA. Translation: BAA35924.1 .
    PIRi A00461. DEECR.
    RefSeqi NP_415627.1. NC_000913.3.
    YP_489377.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OZK model - A 2-386 [» ]
    ProteinModelPortali P00393.
    SMRi P00393. Positions 2-400.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10325N.
    IntActi P00393. 9 interactions.
    STRINGi 511145.b1109.

    Protein family/group databases

    PeroxiBasei 5949. EcoNadDH_K12.

    Proteomic databases

    PaxDbi P00393.
    PRIDEi P00393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74193 ; AAC74193 ; b1109 .
    BAA35924 ; BAA35924 ; BAA35924 .
    GeneIDi 12931088.
    946792.
    KEGGi ecj:Y75_p1079.
    eco:b1109.
    PATRICi 32117461. VBIEscCol129921_1153.

    Organism-specific databases

    EchoBASEi EB0643.
    EcoGenei EG10649. ndh.

    Phylogenomic databases

    eggNOGi COG1252.
    HOGENOMi HOG000157010.
    KOi K03885.
    OMAi ALHGVFK.
    OrthoDBi EOG6QZMR6.
    PhylomeDBi P00393.

    Enzyme and pathway databases

    BioCyci EcoCyc:NADH-DHII-MONOMER.
    ECOL316407:JW1095-MONOMER.
    MetaCyc:NADH-DHII-MONOMER.

    Miscellaneous databases

    PROi P00393.

    Gene expression databases

    Genevestigatori P00393.

    Family and domain databases

    InterProi IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon."
      Young J.G., Rogers B.L., Campbell H.D., Jaworowski A., Shaw D.C.
      Eur. J. Biochem. 116:165-170(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiDHNA_ECOLI
    AccessioniPrimary (citable) accession number: P00393
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3