Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P00393 (DHNA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase

EC=1.6.99.3
Gene names
Name:ndh
Ordered Locus Names:b1109, JW1095
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation.

Catalytic activity

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cell membrane.

Induction

Expressed aerobically and repressed in fermentation.

Sequence similarities

Belongs to the NADH dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 434433NADH dehydrogenase
PRO_0000079890

Regions

Nucleotide binding7 – 4236FAD By similarity
Nucleotide binding171 – 20838NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P00393 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3C3F4FA0E31E10E1

FASTA43447,359
        10         20         30         40         50         60 
MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL LHEVATGSLD 

        70         80         90        100        110        120 
EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR DEKGELLVPE RKIAYDTLVM 

       130        140        150        160        170        180 
ALGSTSNDFN TPGVKENCIF LDNPHQARRF HQEMLNLFLK YSANLGANGK VNIAIVGGGA 

       190        200        210        220        230        240 
TGVELSAELH NAVKQLHSYG YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG 

       250        260        270        280        290        300 
VRVLTQTMVT SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP 

       310        320        330        340        350        360 
TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN GKPLKNYQYK 

       370        380        390        400        410        420 
DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL YRMHQIALHG YFKTGLMMLV 

       430 
GSINRVIRPR LKLH 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon."
Young J.G., Rogers B.L., Campbell H.D., Jaworowski A., Shaw D.C.
Eur. J. Biochem. 116:165-170(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20.
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00306 Genomic DNA. Translation: CAA23586.1.
U00096 Genomic DNA. Translation: AAC74193.1.
AP009048 Genomic DNA. Translation: BAA35924.1.
PIRDEECR. A00461.
RefSeqNP_415627.1. NC_000913.3.
YP_489377.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OZKmodel-A2-386[»]
ProteinModelPortalP00393.
SMRP00393. Positions 2-400.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10325N.
IntActP00393. 9 interactions.
STRING511145.b1109.

Protein family/group databases

PeroxiBase5949. EcoNadDH_K12.

Proteomic databases

PaxDbP00393.
PRIDEP00393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74193; AAC74193; b1109.
BAA35924; BAA35924; BAA35924.
GeneID12931088.
946792.
KEGGecj:Y75_p1079.
eco:b1109.
PATRIC32117461. VBIEscCol129921_1153.

Organism-specific databases

EchoBASEEB0643.
EcoGeneEG10649. ndh.

Phylogenomic databases

eggNOGCOG1252.
HOGENOMHOG000157010.
KOK03885.
OMAALHGVFK.
OrthoDBEOG6QZMR6.
PhylomeDBP00393.

Enzyme and pathway databases

BioCycEcoCyc:NADH-DHII-MONOMER.
ECOL316407:JW1095-MONOMER.
MetaCyc:NADH-DHII-MONOMER.

Gene expression databases

GenevestigatorP00393.

Family and domain databases

InterProIPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
ProtoNetSearch...

Other

PROP00393.

Entry information

Entry nameDHNA_ECOLI
AccessionPrimary (citable) accession number: P00393
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene