NADH dehydrogenase - Escherichia coli (strain K12)

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P00393 (DHNA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase
EC=1.6.99.3

Gene names

Name:	ndh
Ordered Locus Names:	b1109, JW1095

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation.
Catalytic activity	NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Cell membrane.
Induction	Expressed aerobically and repressed in fermentation.
Sequence similarities	Belongs to the NADH dehydrogenase family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Ligand	FAD Flavoprotein NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aerobic electron transport chain Inferred from direct assay PubMed 6784762. Source: EcoCyc
Cellular_component	plasma membrane part Inferred from physical interaction PubMed 7020757. Source: EcoCyc
Molecular_function	NADH dehydrogenase (ubiquinone) activity Inferred from direct assay PubMed 7020757. Source: EcoCyc flavin adenine dinucleotide binding Inferred from direct assay PubMed 7020757. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1

Chain

2 – 434

433

NADH dehydrogenase

PRO_0000079890

Regions

Nucleotide binding

7 – 42

FAD By similarity

Nucleotide binding

171 – 208

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P00393 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 3C3F4FA0E31E10E1
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FASTA

434

47,359

        10         20         30         40         50         60 
MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL LHEVATGSLD 

        70         80         90        100        110        120 
EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR DEKGELLVPE RKIAYDTLVM 

       130        140        150        160        170        180 
ALGSTSNDFN TPGVKENCIF LDNPHQARRF HQEMLNLFLK YSANLGANGK VNIAIVGGGA 

       190        200        210        220        230        240 
TGVELSAELH NAVKQLHSYG YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG 

       250        260        270        280        290        300 
VRVLTQTMVT SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP 

       310        320        330        340        350        360 
TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN GKPLKNYQYK 

       370        380        390        400        410        420 
DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL YRMHQIALHG YFKTGLMMLV 

       430 
GSINRVIRPR LKLH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence coding for the respiratory NADH dehydrogenase of Escherichia coli. UUG initiation codon." Young J.G., Rogers B.L., Campbell H.D., Jaworowski A., Shaw D.C. Eur. J. Biochem. 116:165-170(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00306 Genomic DNA. Translation: CAA23586.1.
U00096 Genomic DNA. Translation: AAC74193.1.
AP009048 Genomic DNA. Translation: BAA35924.1.

PIR

DEECR. A00461.

RefSeq

NP_415627.1. NC_000913.2.
YP_489377.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OZK	model	-	A	2-386	[»]

ProteinModelPortal

P00393.

SMR

P00393. Positions 2-426.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10325N.

IntAct

P00393. 9 interactions.

STRING

511145.b1109.

Protein family/group databases

PeroxiBase

5949. EcoNadDH_K12.

Proteomic databases

PaxDb

P00393.

PRIDE

P00393.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74193; AAC74193; b1109.
BAA35924; BAA35924; BAA35924.

GeneID

12931088.
946792.

KEGG

ecj:Y75_p1079.
eco:b1109.

PATRIC

32117461. VBIEscCol129921_1153.

Organism-specific databases

EchoBASE

EB0643.

EcoGene

EG10649. ndh.

Phylogenomic databases

eggNOG

COG1252.

HOGENOM

HOG000157010.

K03885.

OMA

LGSASND.

ProtClustDB

CLSK874586.

Enzyme and pathway databases

BioCyc

EcoCyc:NADH-DHII-MONOMER.
ECOL316407:JW1095-MONOMER.
MetaCyc:NADH-DHII-MONOMER.

Gene expression databases

Genevestigator

P00393.

Family and domain databases

InterPro

IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]

Pfam

PF00070. Pyr_redox. 2 hits.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]

PRINTS

PR00368. FADPNR.

ProtoNet

Search...

Entry information

Entry name

DHNA_ECOLI

Accession

Primary (citable) accession number: P00393

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents