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Protein

NADPH--cytochrome P450 reductase

Gene

POR

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

Protein has several cofactor binding sites:

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi171 – 20232FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi315 – 32612FADBy similarityAdd
BLAST
Nucleotide bindingi452 – 46211FADBy similarityAdd
BLAST
Nucleotide bindingi530 – 54819NADPBy similarityAdd
BLAST
Nucleotide bindingi625 – 64117NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14302.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Cleaved into the following chain:
Gene namesi
Name:POR
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

  1. Endoplasmic reticulum membrane; Peripheral membrane protein

  2. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 679679NADPH--cytochrome P450 reductasePRO_0000167599Add
BLAST
Initiator methioninei1 – 11Removed; alternateBy similarity
Chaini2 – 679678NADPH--cytochrome P450 reductase, N-terminally processedPRO_0000421789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000020921.

Structurei

3D structure databases

ProteinModelPortaliP00389.
SMRiP00389. Positions 65-679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 225145Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini280 – 522243FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP00389.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSHGDTGA TMPEAAAQEA SVFSMTDVVL FSLIVGLITY WFLFRKKKEE
60 70 80 90 100
VPEFTKIQAP TSSSVKESSF VEKMKKTGRN IVVFYGSQTG TAEEFANRLS
110 120 130 140 150
KDAHRYGMRG MAADPEEYDL ADLSSLPEIN NALAVFCMAT YGEGDPTDNA
160 170 180 190 200
QDFYDWLQET DVDLSGVKYA VFGLGNKTYE HFNAMGKYVD QRLEQLGAQR
210 220 230 240 250
IFELGMGDDD ANLEEDFITW REQFWPAVCE HFGVEATGEE SSIRQYELVL
260 270 280 290 300
HTDIDVAKVY QGEMGRLKSY ENQKPPFDAK NPFLATVTTN RKLNQGTERH
310 320 330 340 350
LMHLELDISD SKIRYESGDH VAVYPANDSA LVNQLGEILG ADLDVVMSLN
360 370 380 390 400
NLDEESNKKH PFPCPTSYRT ALTYYLDITN PPRTNVLYEL AQYAADPAEQ
410 420 430 440 450
EQLRKMASSS GEGKELYLSW VVEARRHILA ILQDYPSLRP PIDHLCELLP
460 470 480 490 500
RLQARYYSIA SSSKVHPNSV HICAVAVEYE TKAGRLNKGV ATSWLRAKEP
510 520 530 540 550
AGENGGRALV PMFVRKSQFR LPFKATTPVI MVGPGTGVAP FIGFIQERAW
560 570 580 590 600
LRQQGKEVGE TLLYYGCRRA AEDYLYREEL AGFQKDGTLS QLNVAFSREQ
610 620 630 640 650
AQKVYVQHLL RRDKEHLWRL IHEGGAHIYV CGDARNMARD VQNTFYDIVA
660 670
ELGAMEHAQA VDYVKKLMTK GRYSLDVWS
Length:679
Mass (Da):76,588
Last modified:July 21, 1986 - v1
Checksum:iB1A163FA53A5988B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 412YW → NY AA sequence (PubMed:6802823).Curated
Sequence conflicti53 – 531E → N AA sequence (PubMed:6802823).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00101 mRNA. Translation: BAA00063.1.
X04610 mRNA. Translation: CAA28279.1.
PIRiA25505.
RefSeqiNP_001153762.1. NM_001160290.1.
UniGeneiOcu.2197.

Genome annotation databases

GeneIDi100301554.
KEGGiocu:100301554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00101 mRNA. Translation: BAA00063.1.
X04610 mRNA. Translation: CAA28279.1.
PIRiA25505.
RefSeqiNP_001153762.1. NM_001160290.1.
UniGeneiOcu.2197.

3D structure databases

ProteinModelPortaliP00389.
SMRiP00389. Positions 65-679.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000020921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100301554.
KEGGiocu:100301554.

Organism-specific databases

CTDi5447.

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP00389.
KOiK00327.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14302.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequence analysis of full-length cDNA for rabbit liver NADPH-cytochrome P-450 reductase mRNA."
    Katagiri M., Murakami H., Yabusaki Y., Sugiyama T., Okamoto M., Yamano T., Ohkawa H.
    J. Biochem. 100:945-954(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structural features of liver microsomal NADPH-cytochrome P-450 reductase. Hydrophobic domain, hydrophilic domain, and connecting region."
    Black S.D., Coon M.J.
    J. Biol. Chem. 257:5929-5938(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-81.
    Tissue: Liver.

Entry informationi

Entry nameiNCPR_RABIT
AccessioniPrimary (citable) accession number: P00389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.