Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADPH--cytochrome P450 reductase

Gene

POR

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091FMNUniRule annotation
Binding sitei299 – 2991NADPUniRule annotation
Binding sitei425 – 4251FADUniRule annotation
Binding sitei479 – 4791FADUniRule annotation
Binding sitei536 – 5361NADPUniRule annotation
Binding sitei640 – 6401NADPUniRule annotation
Binding sitei678 – 6781FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 926FMNUniRule annotation
Nucleotide bindingi139 – 1424FMNUniRule annotation
Nucleotide bindingi174 – 18310FMNUniRule annotation
Nucleotide bindingi455 – 4584FADUniRule annotation
Nucleotide bindingi473 – 4753FADUniRule annotation
Nucleotide bindingi489 – 4924FADUniRule annotation
Nucleotide bindingi597 – 5982NADPUniRule annotation
Nucleotide bindingi603 – 6075NADPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14302.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PORUniRule annotation
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2120LumenalUniRule annotationAdd
BLAST
Transmembranei22 – 4221HelicalUniRule annotationAdd
BLAST
Topological domaini43 – 679637CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 679678NADPH--cytochrome P450 reductasePRO_0000167599Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP00389.
SMRiP00389. Positions 65-679.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 225145Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini280 – 522243FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP00389.
KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSHGDTGA TMPEAAAQEA SVFSMTDVVL FSLIVGLITY WFLFRKKKEE
60 70 80 90 100
VPEFTKIQAP TSSSVKESSF VEKMKKTGRN IVVFYGSQTG TAEEFANRLS
110 120 130 140 150
KDAHRYGMRG MAADPEEYDL ADLSSLPEIN NALAVFCMAT YGEGDPTDNA
160 170 180 190 200
QDFYDWLQET DVDLSGVKYA VFGLGNKTYE HFNAMGKYVD QRLEQLGAQR
210 220 230 240 250
IFELGMGDDD ANLEEDFITW REQFWPAVCE HFGVEATGEE SSIRQYELVL
260 270 280 290 300
HTDIDVAKVY QGEMGRLKSY ENQKPPFDAK NPFLATVTTN RKLNQGTERH
310 320 330 340 350
LMHLELDISD SKIRYESGDH VAVYPANDSA LVNQLGEILG ADLDVVMSLN
360 370 380 390 400
NLDEESNKKH PFPCPTSYRT ALTYYLDITN PPRTNVLYEL AQYAADPAEQ
410 420 430 440 450
EQLRKMASSS GEGKELYLSW VVEARRHILA ILQDYPSLRP PIDHLCELLP
460 470 480 490 500
RLQARYYSIA SSSKVHPNSV HICAVAVEYE TKAGRLNKGV ATSWLRAKEP
510 520 530 540 550
AGENGGRALV PMFVRKSQFR LPFKATTPVI MVGPGTGVAP FIGFIQERAW
560 570 580 590 600
LRQQGKEVGE TLLYYGCRRA AEDYLYREEL AGFQKDGTLS QLNVAFSREQ
610 620 630 640 650
AQKVYVQHLL RRDKEHLWRL IHEGGAHIYV CGDARNMARD VQNTFYDIVA
660 670
ELGAMEHAQA VDYVKKLMTK GRYSLDVWS
Length:679
Mass (Da):76,588
Last modified:July 21, 1986 - v1
Checksum:iB1A163FA53A5988B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 412YW → NY AA sequence (PubMed:6802823).Curated
Sequence conflicti53 – 531E → N AA sequence (PubMed:6802823).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00101 mRNA. Translation: BAA00063.1.
X04610 mRNA. Translation: CAA28279.1.
PIRiA25505.
RefSeqiNP_001153762.1. NM_001160290.1.
UniGeneiOcu.2197.

Genome annotation databases

GeneIDi100301554.
KEGGiocu:100301554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00101 mRNA. Translation: BAA00063.1.
X04610 mRNA. Translation: CAA28279.1.
PIRiA25505.
RefSeqiNP_001153762.1. NM_001160290.1.
UniGeneiOcu.2197.

3D structure databases

ProteinModelPortaliP00389.
SMRiP00389. Positions 65-679.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100301554.
KEGGiocu:100301554.

Organism-specific databases

CTDi5447.

Phylogenomic databases

HOGENOMiHOG000282027.
HOVERGENiHBG000432.
InParanoidiP00389.
KOiK00327.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14302.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequence analysis of full-length cDNA for rabbit liver NADPH-cytochrome P-450 reductase mRNA."
    Katagiri M., Murakami H., Yabusaki Y., Sugiyama T., Okamoto M., Yamano T., Ohkawa H.
    J. Biochem. 100:945-954(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structural features of liver microsomal NADPH-cytochrome P-450 reductase. Hydrophobic domain, hydrophilic domain, and connecting region."
    Black S.D., Coon M.J.
    J. Biol. Chem. 257:5929-5938(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 12-81.
    Tissue: Liver.

Entry informationi

Entry nameiNCPR_RABIT
AccessioniPrimary (citable) accession number: P00389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 6, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.