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Protein

NADPH--cytochrome P450 reductase

Gene

POR

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209FMNUniRule annotation1
Binding sitei299NADPUniRule annotation1
Binding sitei425FADUniRule annotation1
Binding sitei479FADUniRule annotation1
Binding sitei536NADPUniRule annotation1
Binding sitei640NADPUniRule annotation1
Binding sitei678FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi87 – 92FMNUniRule annotation6
Nucleotide bindingi139 – 142FMNUniRule annotation4
Nucleotide bindingi174 – 183FMNUniRule annotation10
Nucleotide bindingi455 – 458FADUniRule annotation4
Nucleotide bindingi473 – 475FADUniRule annotation3
Nucleotide bindingi489 – 492FADUniRule annotation4
Nucleotide bindingi597 – 598NADPUniRule annotation2
Nucleotide bindingi603 – 607NADPUniRule annotation5

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14302

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PORUniRule annotation
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21LumenalUniRule annotationAdd BLAST21
Transmembranei22 – 42HelicalUniRule annotationAdd BLAST21
Topological domaini43 – 679CytoplasmicUniRule annotationAdd BLAST637

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001675991 – 679NADPH--cytochrome P450 reductaseAdd BLAST679

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP00389
SMRiP00389
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 225Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini280 – 522FAD-binding FR-typeUniRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000282027
HOVERGENiHBG000432
InParanoidiP00389
KOiK00327

Family and domain databases

Gene3Di1.20.990.10, 4 hits
3.40.50.360, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

P00389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSHGDTGA TMPEAAAQEA SVFSMTDVVL FSLIVGLITY WFLFRKKKEE
60 70 80 90 100
VPEFTKIQAP TSSSVKESSF VEKMKKTGRN IVVFYGSQTG TAEEFANRLS
110 120 130 140 150
KDAHRYGMRG MAADPEEYDL ADLSSLPEIN NALAVFCMAT YGEGDPTDNA
160 170 180 190 200
QDFYDWLQET DVDLSGVKYA VFGLGNKTYE HFNAMGKYVD QRLEQLGAQR
210 220 230 240 250
IFELGMGDDD ANLEEDFITW REQFWPAVCE HFGVEATGEE SSIRQYELVL
260 270 280 290 300
HTDIDVAKVY QGEMGRLKSY ENQKPPFDAK NPFLATVTTN RKLNQGTERH
310 320 330 340 350
LMHLELDISD SKIRYESGDH VAVYPANDSA LVNQLGEILG ADLDVVMSLN
360 370 380 390 400
NLDEESNKKH PFPCPTSYRT ALTYYLDITN PPRTNVLYEL AQYAADPAEQ
410 420 430 440 450
EQLRKMASSS GEGKELYLSW VVEARRHILA ILQDYPSLRP PIDHLCELLP
460 470 480 490 500
RLQARYYSIA SSSKVHPNSV HICAVAVEYE TKAGRLNKGV ATSWLRAKEP
510 520 530 540 550
AGENGGRALV PMFVRKSQFR LPFKATTPVI MVGPGTGVAP FIGFIQERAW
560 570 580 590 600
LRQQGKEVGE TLLYYGCRRA AEDYLYREEL AGFQKDGTLS QLNVAFSREQ
610 620 630 640 650
AQKVYVQHLL RRDKEHLWRL IHEGGAHIYV CGDARNMARD VQNTFYDIVA
660 670
ELGAMEHAQA VDYVKKLMTK GRYSLDVWS
Length:679
Mass (Da):76,588
Last modified:July 21, 1986 - v1
Checksum:iB1A163FA53A5988B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40 – 41YW → NY AA sequence (PubMed:6802823).Curated2
Sequence conflicti53E → N AA sequence (PubMed:6802823).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00101 mRNA Translation: BAA00063.1
X04610 mRNA Translation: CAA28279.1
PIRiA25505
RefSeqiNP_001153762.1, NM_001160290.1
UniGeneiOcu.2197

Genome annotation databases

GeneIDi100301554
KEGGiocu:100301554

Similar proteinsi

Entry informationi

Entry nameiNCPR_RABIT
AccessioniPrimary (citable) accession number: P00389
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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