P00386 (DHNO_AGRT7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-nopaline dehydrogenase
EC=1.5.1.19

Alternative name(s):
Nopaline synthase

Gene names

Name:

nos

Encoded on

Plasmid pTiT37

Organism

Agrobacterium tumefaciens (strain T37)

Taxonomic identifier

176300 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Ontologies

Keywords
Disease	Crown gall tumor
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	D-nopaline dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	D-nopaline dehydrogenase		PRO_0000179983
Experimental info
	Sequence conflict	222	1	C → Y Ref.2
	Sequence conflict	359	1	N → K Ref.2
	Sequence conflict	362	1	L → I Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P00386 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 9639D2D73C57CEF8	FASTA	413	45,387
	10 20 30 40 50 60 MAITLSATSL PISAADHHPL PLTVGVLGSG HAGTALAAWF ASRHVPTALW APADHPGSIS 70 80 90 100 110 120 AIKANEGVIT TEGMINGPFR VSACDDLAAV IRSSRVLIIV TRADVHDSFV NELANFNGEL 130 140 150 160 170 180 ATKDIVVVCG HGFSIKYERQ LRFKRIFETD NSPITSKLSD QKKCNVNIKE MKASFGLSCF 190 200 210 220 230 240 PIHRDDAGVI DLPEDTKNIF AQLFSARIIC IPPLQVLFFS NCITHAVPAV MNIGRLRDPA 250 260 270 280 290 300 NSLTKRAEKW LLELDERTPR AEKGFFFYGE GSNTYVCNVQ EQIDHERRKV AAACGLRLNS 310 320 330 340 350 360 LLQECNDEYD TDYETLREYC LAPSPHNVHH ACPDNMEHRY FSEELCSLED VAAIAAIANI 370 380 390 400 410 ELPLTHAFIN IIHAGKGKIN PTGKSSSVIG NFSSSDLIRF GATHVFNKDE MVE

References

[1]	"Nopaline synthase: transcript mapping and DNA sequence." Depicker A., Stachel S., Dhaese P., Zambryski P., Goodman H.M. J. Mol. Appl. Genet. 1:561-573(1982) [PubMed: 7153689] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and transcription of the nopaline synthase gene region of T-DNA." Bevan M., Barnes W.M., Chilton M.-D. Nucleic Acids Res. 11:369-385(1983) [PubMed: 6298724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00087 Genomic DNA. Translation: CAA23427.1.
PIR	DEAGNT. A92833.
3D structure databases
ProteinModelPortal	P00386.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR011128. G3P_DH_NAD-dep_N. IPR016040. NAD(P)-bd_dom. IPR003421. Opine_DH. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01210. NAD_Gly3P_dh_N. 1 hit. PF02317. Octopine_DH. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DHNO_AGRT7

Accession

Primary (citable) accession number: P00386

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 28, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program