Reviewed, UniProtKB/Swiss-Prot P00384 (DYR22_ECOLX)

Names and origin

Protein names	Recommended name:     Dihydrofolate reductase type 2     EC=1.5.1.3 Alternative name(s):     Dihydrofolate reductase type II
Encoded on	Plasmid IncW R388
Organism	Escherichia coli
Taxonomic identifier	562 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	Type II plasmid-specified enzyme is practically insensitive to trimethoprim and methotrexate. The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to drug Inferred from electronic annotation. Source: InterPro response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plastid Inferred from electronic annotation. Source: InterPro
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 78	78	Dihydrofolate reductase type 2		PRO_0000186436

Sequences

	Sequence		Length	Mass (Da)
	P00384-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: EE9B686CBE5BCB93	FASTA	78	8,195
	10 20 30 40 50 60 MGQSSDEANA PVAGQFALPL SATFGLGDRV RKKSGAAWQG QVVGWYCTKL TPEGYAVESE 70 SHPGSVQIYP VAALERVA

References

[1]	"Characterization of a R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene." Zolg J.W., Haenggi U.J. Nucleic Acids Res. 9:697-710(1981) [PubMed: 6261228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence of a plasmid-encoded dihydrofolate reductase." Swift G., McCarthy B.J., Heffron F. Mol. Gen. Genet. 181:441-447(1981) [PubMed: 7022127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	V00252 Genomic DNA. Translation: CAA23503.1. J01773 Genomic DNA. Translation: AAA72145.1.
PIR	RDECD8. A00398.
3D structure databases
HSSP	HSSP built from PDB template 1VIE based on UniProtKB P00383.
SMR	P00384. Positions 19-77.
ModBase	Search...
Family and domain databases
InterPro	IPR009159. Dhfr_type_II. [Graphical view]
Pfam	PF06442. DHFR_2. 1 hit. [Graphical view]
PIRSF	PIRSF000199. Dhfr_type_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DYR22_ECOLX

Accession

Primary (citable) accession number: P00384

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)